Adelbert Adrian A. Sendon

University of the Philippines Los Baños

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Freddiewebb B. Signabon

University of the Philippines Los Baños

Johnny F. Balidion

University of the Philippines Los Baños

Jaymee R. Encabo

University of the Philippines Los Baños

Benji Brayan Ilagan Silva

National Pingtung University of Science and Technology

Rae Mark L. Mercado

University of the Philippines Los Baños

Aeron Jade S. Parena

University of the Philippines Los Baños

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University of the Philippines Los Baños

International Rice Research Institute

Hokkaido University

University of the Philippines System

National Pingtung University of Science and Technology

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Lytic phages display protective effects against soft rot-causing Pectobacterium sp.

Biocontrol Science and Technology (2022)

Aeron Jade S. Parena Benji Brayan Ilagan Silva Rae Mark L. Mercado Adelbert Adrian A. Sendon Freddiewebb B. Signabon

Soft rot caused by Pectobacterium sp. is responsible for significant losses in vegetable production worldwide. Methods for the effective control of soft rot are limited and primarily based on good agricultural practices. The use of bacteriophages as biocontrol agents appears to be a promising alternative to combat phytopathogens. In this study, we investigated the efficacy of lytic phages recovered from symptomatic cabbage tissues and environmental samples against soft rot caused by Pectobacterium carotovorum subsp. carotovorum. Three bacteriophage isolates, designated as vB_PcaP-A3, vB_PcaM-D1, and vB_PcaM-J3, were observed to effectively lyse P. carotovorum subsp. carotovorum. Phage vB_PcaP-A3 exhibited virion morphology similar to the members of the podovirus group, while phages vB_PcaM-D1 and vB_PcaM-J3 showed myovirus morphology based on transmission electron microscopy. The optimal multiplicity of infection (MOI) differed greatly among the three phages. All three phages survived incubations at 30, 40 and 50 °C and pH conditions ranging from 3.0 to 9.0, but were all inactivated at 60°C and at pH 12. Both monophage and cocktail preparations were effective in inhibiting the growth of P. carotovorum subsp. carotovorum in the in vitro challenge tests. In the potato slice assays, while treatment with cocktail preparations completely inhibited the development of soft rot in tissue slices, monophage treatments not only resulted in a significant reduction of tissue maceration in slices, but also showed protective effect against soft rot in tubers. Overall, these results demonstrate the efficacy of phages vB_PcaP-A3, vB_PcaM-D1, and vB_PcaM-J3 for the biocontrol of soft rot caused by P. carotovorum subsp. carotovorum.

Pectobacterium carotovorum

Lytic cycle

Pectobacterium

10.1080/09583157.2022.2122403

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Lytic phages displayed protective effects against soft rot-causing Pectobacterium sp.

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Aeron Jade S. Parena Benji Brayan Ilagan Silva Rae Mark L. Mercado Adelbert Adrian A. Sendon Freddiewebb B. Signabon

Abstract Soft rot caused by Pectobacterium sp. is responsible for significant losses in vegetable production worldwide. Methods for the effective control of soft rot are limited and are primarily based on good agricultural practices. The use of bacteriophages as biocontrol agents appears to be a promising alternative to combat phytopathogens. In this study, we investigated the efficacy of lytic phages recovered from symptomatic tissues and environmental samples against soft rot caused by Pectobacterium carotovorum subsp. carotovorum . Three bacteriophage isolates, designated as vB_PcaP-A3, vB_PcaM-D1, and vB_PcaM-J3, were observed to effectively lyse P. carotovorum subsp. carotovorum . Phage vB_PcaP-A3 exhibited virion morphology similar to the members of the podovirus group, while phages vB_PcaM-D1 and vB_PcaM-J3 showed myovirus morphology based on transmission electron microscopy. The optimal multiplicity of infection (MOI) differed greatly among the three phages. All three phages survived incubations at 30°C, 40°C and 50°C and pH conditions ranging from 3.0 to 9.0, but were all inactivated at 60°C and at pH 12. Both monophage and cocktail preparations were effective in inhibiting the growth of P. carotovorum subsp. carotovorum in the in vitro challenge tests. In the semi- in planta assays, while treatment with cocktail preparations completely inhibited the development of soft rot in tissue slices, monophage treatments not only resulted in significant reduction of tissue maceration in slices, but also showed protective effect against soft rot in tubers. Overall, these results demonstrate the efficacy of phages vB_PcaP-A3, vB_PcaM-D1, and vB_PcaM-J3 for the biocontrol of soft rot caused by P. carotovorum subsp. carotovorum .

Pectobacterium carotovorum

Lytic cycle

Pectobacterium

10.1101/2021.11.15.468558

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