Rafid M. Karim

University of Basrah

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ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISM RS9939609 IN FTO GENE OF OBESE MALES IN IRAQI POPULATION

Iraqi journal of Medical Sciences (2016)

Mustafa Jumaa Nahi Y. Yaseen Adil Shehab Rafid M. Karim Likaa Sagban

Background Obesity is a serious on public health.It contributes in many serious health conditions including high cholesterol, type 2 diabetes, osteoarthritis, high blood pressure, gallbladder disease, coronary heart disease, stroke, respiratory problems … etc. Polymorphism of TA (rs9939609) in fat mass and obesity associated (FTO) gene was found to be associated with obesity in children and adults according to many studies conducted on populations from Europe, America and Asia.Whether Single nucleotide polymorphism (SNP) rs9939609 is an associated with obesity in Iraqi population remains of concern. ObjectiveTo investigate the polymorphism of rs9939609 SNP in FTO gene and its relationship to the obese males in Iraqi population. MethodsOne hundred twenty of males were classified as obese based on body mass index (BMI) with mean age 20-50 year and fifty aged-matched healthy males as a control were included in this study.Lipid profile was estimated by using Spinreact-Сє, and an ELISA kit was used to assess the FTO level. ResultsThe results showed that there are significant differences P ≤ 0.05 for AA genotype with all parameters whereas TA genotype showed significant differences with most of parameters in revers to TT genotype, which has showed no significant differences with most of parameters.The percentage of TT, TA, AA, alleles were 27.72%, 49.86%, 22.42% respectively, also an elevated of TT genotype frequency was observed in healthy compared to obese.On the other hand, the percentage of T and A allele frequency were 52.65% and 47.35% respectively.Also, an elevated in serum FTO enzyme level was observed in obese. ConclusionThe presence of A risk allele in the Iraqi population is the cause in the incidence of obesity, which reflected its impact on the BMI and central obesity through the disturbances in lipid profile and FTO enzymes value.

FTO gene

10.22578/ijms.14.3.9

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Citations (2)

The density and reproductive biology of the Fiddler Crab Uca sindensis (Alcock, 1900) (Crustacea: Ocypodidae) in intertidal zone of Shatt Al-Basrah river –Khor Al-Zubair, Iraq.

Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection (2017)

Khalid Kh. S. Alkhafaji Rafid M. Karim Rajaa Abdul – Kadem haneff Mustafa Jumaa Hind Hussein Obaid

The density and reproductive biology of Uca sindensis were studied inintertidal zone of Khor Al-Zubair, south of Shatt Al-Basrah river, Basrah, Iraq.The crabs were collected randomly three 0.25m2 quadrates during low tideperiods from November 2014 to October 2015. Density of crabs, sex ratio,distribution, population structure, diameter burrow, breeding season andfecundity were investigated. The results showed that crabs population fluctuatedand depended on the months of the year. Crabs density in study area was(181ind/ m2 in April 2015) and (19 ind./m2 in December 2015). The CW offemales ranged from 4 to 13.5 mm and of males from 5 to 16 mm indicatingsexual dimorphism. The relationship between Carapace width (CW) × Carapace height (CH) was positive in males and females. The equation (Log CH=Log-4.644+4.362LogCW) for males (P<0.05) and Log CH=Log-2.152+3.299LogCW for females (P<0.05). Sex ratio showed lower number of females than males forstudy area, The overall sex ratio was 1:1.26 and did not differ significantlyfrom the expected 1:1 (χ test, P>0.05), however the monthly sex ratio wasdifferent from expected 1:1(χ test, P<0.05). Breeding took place during warmseason of March to October 2015. The regression analysis showed that thenumber of eggs increased linearly with the increase of carapace width (r2=0.775, n=38). It was concluded that some factors of temperature, Salinity andTOM in sediment affected at density growth and reproduction pattern in U.sindensis.

Ocypodidae

Fiddler crab

Population density

Chela

10.24271/garmian.146

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Citations (3)

Lipid peroxidation and enzymatic activity levels in Corbicula fluminalis from two sites of Shatt Al-Arab

F1000Research (2014)

Rafid M. Karim Mustafa Nuhad Al-Darraji

A suite of biomarkers including Lipid peroxidation (LP), activities of Superoxide dismutase (SOD) and Catalase (CAT) enzymes were studied in gills and digestive glands of Corbicula fluminalis from two sites of Shatt Al-Arab (Qurna & Al-Maaqil regions). The values of LP, SOD and CAT were higher in gills as well as in digestive glands in Corbicula fluminalis from Al-Maaqil as compared with that animal from Qurna region, these indicators gave the impression that AL-Maaqil region was exposed to pollutants which lead to rising antioxidant levels in clam Corbicula fluminalis. Keywords—Lipid peroxidation; Superoxide dismutase; Corbicula fluminalis

10.7490/f1000research.1096537.1

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Evaluation of DNA Damage of Cadmium in Gastropod Lymnaea radix cor (Annandale and Prashad, 1919) From Euphrates River

Research Journal of Pharmacy and Technology (2022)

Rafid M. Karim

Cadmium toxicity to a snail Lymnaea radix cor collected from Shatt Almadina stream that belongs to Euphrates River, Basra province, Iraq was investigated. DNA damage was used as an indicator for cadmium toxicity in the current study. The adult snails were exposed to several concentrations of cadmium for different periods. DNA fragmentation occurring in the snail exposed to cadmium was substantiated by comet assay and expressed in term Tail DNA percentage. Results revealed a significant increase in DNA damage at cadmium concentrations 60, 80, and 100µg/l for an exposure time of 3 days, and the highest percentage of tail DNA was at the cadmium concentration of 100µg/l. On the other hand, the DNA damage significantly elevated when the exposure period increased to 5 days and the highest tail DNA percentage (18%) was recorded at cadmium concentration 100 µg/l. the assessment of DNA damage in the freshwater snail Lymnaea radix cor gives an early caution signal for aquatic environmental contamination by cadmium.

Lymnaea stagnalis

Comet Assay

Freshwater snail

10.52711/0974-360x.2022.00462

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Cytotoxic Effect of Vincarosea Aqueous Crude Extraction Human Brain Carcinoma Cell Line (AMGM) In Vitro

Engineering and Technology Journal (2016)

Liqaa H. Saqban Hind Hussein Obaid Dalia Azhar Ahmed Dimah N. Passat Mustafa Nuhad Al-Darraji

The present study investigated the cytotoxic effects of aqueous crude extracts of Vincarosea leaves, flowers and seeds on Human brain carcinoma cell line (AMGA, Ahmed Majeed Glioblastoma Multiform ) in vitro, by using serial double dilution (concentration between 1.95-1000 µg/ml). The results showed that the cytotoxic effect of extracts was depended on type of parts of plant extracted, concentration and exposure time. The concentration 1000 µg/ml gave inhibition rate (IR), were (34, 49 and 64) % of leaves, flowers and seeds extracts respectively compared with control 100% after 24 hours from exposure time. However, low concentrations of aqueous extracts were found to induce the AMGA cells growth and proliferation (PR), it was 115% by treatment with aqueous extract offlowers extract in 1.95 µg/ml after 24 hours of exposed.

Aqueous extract

10.30684/etj.34.3b.21

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Citations (1)

Spectral Properties, Reconstitution and Kinetics of Paracoccous denitrificans Isovaleryl-CoA Dehydrogenase

Iraqi Journal of Science (2016)

Rafid M. Karim Abdulkareem Jasim Hashim

Paracoccus denitrificans

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SURVEY OF AEROMONAS HYDROPHILA IN THREE MARINE FISH SPECIES FROM NORTH WEST ARABIAN GULF, IRAQ

Basrah Journal of veterinary Research (2011)

Ghazi M. Al-Maleky Rafid M. Karim Amjed N. Al-Abresm

Marine fish

West coast

10.33762/bvetr.2011.55028

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Citations (6)

Characterization of B-lactamase from local isolate of Staphylococcus aureus

Journal of Techniques (2010)

Awatif H. Issa Eiman A. Saeed Rafid M. Karim

Source

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Lipid peroxidation and alkaline phosphotase levels in gastropod Lymnaea Radix cor (Annandale and Prashad, 1919 ) exposed to sublethal concentrations of endosulfan

مجلة علوم الحياة | Al-Kufa Journal for ِBiology (2011)

Rafid M. Karim Murtada D. Naser Adnan J.M. Al Fartosi

The gastropod Lymnaea Radix cor was exposed to sub lethal concentrations ( 22.75 and 45.5 µg/L ) of endosulfan for 8 days. Important biomarkers were assayed in digestive glands during exposure intervals ( 2, 4 and 8 days ). Increasing in lipid peroxidation, markers including Malondialdehyde (MDA), was observed after 2 days of exposure with 22.75 µg/L endosulfan then the increasing became lower in 4 th day and nonsignificant differences between 4 th and 8 th days of exposure were noticed. while the increase in lipid peroxidation was greater after 2 days of exposure with 45.5 µg/L endosulfan than that at 22.75 µg/L. The results showed decrease in Alkaline phosphatase enzyme in second day of exposure with 22.75 µg/L endosulfan whereas more decreasing in ALP were observed after 2 day of exposure with 45.5 µg/L endosulfan.

Endosulfan

Malondialdehyde

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Identification of Active Site Catalytic Residue in Isovaleryl-CoA Dehydrogenase from Paracoccus denitrificans Pd1222

Rafid M. Karim Abdulkareem Jasim Hashim

The gene of Pden_3633 in Paracoccus denitrificans Pd1222, Isovaleryl-CoA dehydrogenase gene (IVDH), was synthesized, cloned, expressed into E. coli BL21 (DE3) using pET24d vector, and purified as N-terminal Strep-Tagged enzyme (Karim and Hashim, 2016a; Karim and Hashim; 2016b). In current study, a Site-directed mutagenesis was used to identify the active site catalytic residue of this synthetic Sterp-Tag IVDH enzyme. Amino acid alignment showed that the E246 is the predicted active site catalytic residue. To substantiate the role of E246 as a catalytic residue, a mutant E246Q IVDH was constructed. Spectral properties of the mutant IVDH indicated that it was obtained as an apoprotein. Therefore, the protein was full reconstituted by incubation with flavin adenine dinucleotide (FAD) at a ratio 1: 20% (IVDH: FAD) molar excess. The results revealed that the reconstituted E246Q IVDH had no activity for isovaleryl-CoA. Furthermore, its UV/visible spectrum resulted from titration with isovaleryl-CoA did not induce quenching of the absorption at 364 and 440 nm regions or arise a new absorption at 598 nm as wild type did. Confirming that the mutant IVDH was unable to form charge transfer complex as a result of altering E246 and the later is the active site catalytic residue of P. denitrificans IVDH

Paracoccus denitrificans

Residue (chemistry)

Site-directed mutagenesis

Flavoprotein

10.24271/garmian.scpas27

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