Roberto Elias Villela Miguel

Hospital Sírio-Libanês

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Luiz Paulo Kowalski

AC Camargo Hospital

Helena Brentani

Universidade de São Paulo

Orlando Parise

Maastricht University

ÂF

Ângela Flávia Logullo

Universidade Federal de São Paulo

Maria Mitzi Brentani

Instituto do Câncer do Estado de São Paulo

Leda Viegas de Carvalho

Hospital Garcia de Orta

Inês Nobuko Nishimoto

AC Camargo Hospital

Suely Nonogaki

Instituto Adolfo Lutz

Fátima Solange Pasini

Instituto do Câncer do Estado de São Paulo

Miriam Hatsue Honda Federico

Fundação Faculdade de Medicina

Cooperative Institutions

Universidade de São Paulo

Hospital São Paulo

Universidade Federal de São Paulo

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Instituto do Câncer do Estado de São Paulo

AC Camargo Hospital

Hospital Sírio-Libanês

Universidade Brasil

Hospital Heliópolis

Instituto Adolfo Lutz

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Transforming growth factor β1 (TGFβ1) expression in head and neck squamous cell carcinoma patients as related to prognosis

Journal of Oral Pathology and Medicine (2003)

Ângela Flávia Logullo Suely Nonogaki Roberto Elias Villela Miguel Luiz Paulo Kowalski Inês Nobuko Nishimoto

Abstract Background: Transforming growth factor β1 (TGFβ1) is a negative growth regulator in keratinocytes, and in vitro studies lead to the concept that loss of TGFβ1 responsiveness is a critical step in epithelial carcinogenesis. Objective: To investigate the prognostic relevance of TGFβ1 expression in head and neck squamous cell carcinoma (HNSCC). Materials and methods: TGFβ1 distribution was determined by immunohistochemistry in oral cavity/oropharynx ( n = 79), larynx ( n = 36) and hypopharynx ( n = 25) tumors and in matched normal adjacent mucosa. TGFβ‐type I and II receptors were determined in 20 cases of differentiated oral cavity/hypopharynx tumors. Cases were considered positive if displaying reactivity in >10% of the cells. Results: TGFβ1‐positive expression was found in 47.2% of larynx, 36.7% of oral cavity/oropharynx and in 24% of the hypopharynx tumors. Reactivity in >60% of the cells was displayed only by 11.4% of HNSCC. All normal controls were positive. TGFβ1‐positive expression did not correlate with clinico pathological parameters. An association with differentiation was verified only in oral cavity/oropharynx tumors ( P ≤ 0.001). TGFβ1 was also not related to 5 years survival (Kaplan–Meier). Strong and diffuse expression of TGFβ‐RII was identified in 19/20 cases regardless of TGFβ1 immunoreactivity. Out of 17 TGFβ1‐positive oral cavity/oropharynx tumors, only nine expressed TGFβ‐RI suggesting a disruption of the TGFβ1 pathway. We conclude that TGFβ1 protein immunostaining is not a useful biomarker in assessment of prognosis in HNSCC.

Immunostaining

10.1034/j.1600-0714.2003.00012.x

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Low prevalence of human papillomavirus in a geographic region with a high incidence of head and neck cancer

The American Journal of Surgery (1998)

Roberto Elias Villela Miguel Luisa L. Villa Anói Castro Cordeiro Jose Carlos Mann Prado João Simão Sobrinho

Tonsil

Pyriform Sinus

10.1016/s0002-9610(98)00246-3

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Carcinoma Basocelular Metastático: Relato de Caso

Anais Brasileiros de Dermatologia (2001)

José R. L. Lara Roberto Elias Villela Miguel Luiz Paulo Kowalski Luciano Bottura

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P53 overexpression in epidermoid carcinoma of the head and neck

Sao Paulo Medical Journal (1997)

Ângela Flávia Logullo Luiz Paulo Kowalski Sueli Nonogaki Roberto Elias Villela Miguel Humberto Torloni

The theory of field cancerization in tumors of the head and neck reflects the complex oncogenesis that occurs in this region. The mechanisms that control cell proliferation at the molecular level in epidermoid carcinomas (ECs) of the upper aerodigestive tract are still unclear. Mutations in p53 are the genetic alterations most often detected in ECs of the head and neck and seem to contribute actively to the carcinogenic process triggered by p53 as a tumor-suppressor gene and to its association with tobacco. The objective of the present study was to investigate the expression of p53 protein in epidermoid head and neck carcinomas by immunohistochemistry and its immunohistochemical correlation with other prognostic factors. The study was conducted on 63 consecutive ECs cases not submitted to previous treatment. Specimens of the tumor and of the normal adjacent mucosa were collected during surgery and submitted to immunohistochemical reaction for the determination of the expression of anti-protein p53 antibody (M7001 DAKO A/S, Denmark). Anatomo-clinical and demographic data were not significantly correlated with the presence of lymph node metastases or p53 expression in the tumor or in the adjacent normal mucosa. Tumor localization in the larynx was significantly correlated with p53 expression. Histological grading as grades I, II, Ill and IV was correlated with significant p53 expression (p = 0.025). Conclusions: 1) in the studied material obtained from 63 cases of head and neck ECs, we detected a 48 percent rate of immunohistochemically detectable p53 overexpression; 2) we did not detect a relationship between demographic patient data and p53 expression in the tumor or in the normal adjacent mucosa; 3) p53 overexpression was significantly more frequent in ECs material from the larynx; and 4) The presence of 12 cases with p53 overexpression in the normal adjacent mucosa and with a p53-negative tumor is in agreement with the theory of field cancerization. Follow-up of this patient series for a longer period of time will permit a better analysis of these values.

Field cancerization

Epidermoid carcinoma

Grading (engineering)

10.1590/s1516-31801997000100006

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Laryngeal sensitivity evaluation and dysphagia: Hospital Sírio-Libanês experience

Sao Paulo Medical Journal (2004)

Orlando Parise Roberto Elias Villela Miguel Daniela Luci D'Aquino Gomes Antônio Douglas Menon Kiyoshi Hashiba

Laryngeal sensitivity is important in the coordination of swallowing coordination and avoidance of aspiration.

Epiglottis

10.1590/s1516-31802004000500004

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Oral Squamous Cell Carcinoma in an HPV Positive Patient

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology (2018)

Gilberto Marcucci Roberto Elias Villela Miguel Fabiola Settani Yara Menezes VINICIUS PIOLI ZANETIM

Floor of mouth

Incisional biopsy

10.1016/j.oooo.2018.02.417

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C8orf4/TC-1(Thyroid Cancer-1) Gene Expression in Thyroid Cancer and Goiter

ORL (2006)

Poliana Cristina de Melo Martins Orlando Parise Cora Pereira Hors Roberto Elias Villela Miguel Valéria Cristina da Costa Andrade

Background: The expression of the thyroid cancer-1(TC-1) gene seems to be related with malignant transformation in the thyroid tissue. Objective: We evaluated the potential use of TC-1 gene expression as a marker of malignancy in thyroid nodules. Methods: A total of 92 frozen thyroid samples were studied, including 46 samples from thyroid nodules (19 papillary carcinomas, 1 follicular carcinoma, 24 adenomatous goiters, and 2 follicular adenomas) and 46 samples from normal surrounding thyroid tissue. Total RNA was extracted and TC-1 expression was assessed by semiquantitative Multiplex PCR. Results were verified using real-time RT-PCR in some of the samples. Results: Overall mean TC-1 gene expression (normalized by the ABL gene) was 1.73 ± 1.67 (0.33–9.33). There was a significant difference (p < 0.001) between TC-1 gene expression in benign thyroid lesions (1.07 ± 0.10) and carcinomas (2.73 ± 0.51). Conclusion: Our results suggest that TC-1 gene expression may be useful in the differential diagnosis of goiters and thyroid papillary carcinomas.

Thyroid Nodules

10.1159/000097980

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Clinical significance of c-myc and p53 expression in head and neck squamous cell carcinomas

Cancer Detection and Prevention (2004)

Ângela Flávia Logullo Waitzberg Suely Nonogaki Inês Nobuko Nishimoto Luiz Paulo Kowalski Roberto Elias Villela Miguel

Concomitant

P53 expression

10.1016/j.cdp.2004.02.003

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Prognostic impact of p53, c-erbB-2 and epidermal growth factor receptor on head and neck carcinoma

Sao Paulo Medical Journal (2004)

Orlando Parise Leda Viegas de Carvalho Roberto Elias Villela Miguel Luiz Paulo Kowalski

CONTEXT: p53, c-erbB-2 and epidermal growth factor receptor (EGFR) are cancer-related proteins that are usually expressed in head and neck squamous cell carcinoma (SCC). Their prognostic value remains controversial. OBJECTIVE: To evaluate the prognostic impact of p53, c-erbB-2 and EGFR expression in head and neck SCC. TYPE OF STUDY: Prospective. SETTING: Head and Neck Surgery Department, Hospital AC Camargo, São Paulo. METHODS: Fifty-four patients were studied for p53, c-erbB-2 and EGFR expression in head and neck SCC and adjacent mucosa, via immunohistochemistry. These data were correlated with histoclinical data and survival. RESULTS: There was a direct association of p53 expression in SCC and mucosa (p = 0.001); loss of c-erbB-2 expression (-) from normal mucosa to SCC (p = 0.04); lower frequency of association of c-erbB-2 (+) with EGFR (-) in SCC (p = 0.02); and a direct association of EGFR (+) expression in SCC and mitotic index (p = 0.03). The 60-month actuarial survival rates for patients presenting lymph node metastasis were higher when there was no capsule rupture by SCC (48.3%; p = 0.02), no more than one positive lymph node (52.3%; p = 0.004) or clear surgical margins (47.0%; p = 0.01), in comparison with patients presenting capsule rupture (20.2%), two or more positive lymph nodes (18.7%) or compromised surgical margins (0.0%), respectively. Patients presenting SCC p53 (+) and EGFR (-) demonstrated greater survival (75.0%; p = 0.03) than for the remaining group (33.1%). Multivariate analysis confirmed the positive impact of p53 (+) and EGFR (-) on survival (p = 0.02). DISCUSSION: Associations were found for p53, c-erbB-2 and EGFR expression with histoclinical data and prognosis. Interestingly, these results suggest that loss of mucosal c-erbB-2 expression could be involved in SCC carcinogenesis; EGFR expression in SCC is related to tumor mitotic index; and presence of p53 with absence of EGFR expression in head and neck SCC may be a prognostic factor for survival. CONCLUSIONS: Further prospective studies should be conducted to confirm the influence of p53, c-erbB-2 and EGFR on histoclinical data and prognosis.

Epidermoid carcinoma

10.1590/s1516-31802004000600007

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Sarcomas de partes moles de cabeça e pescoço

Roberto Elias Villela Miguel Leda Viegas de Carvalho Luiz Paulo Kowalski

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