Blessing Ogechukwu Umeokoli

Nnamdi Azikiwe University

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Festus Basden Chiedu Okoye

Nnamdi Azikiwe University

Peter M. Eze

Nnamdi Azikiwe University

Wernér E.G. Müller

Johannes Gutenberg University Mainz

Peter Proksch

Heinrich Heine University Düsseldorf

Zhen Liu

Xuzhou Medical College

Cletus Anes Ukwubile

University of Maiduguri

Chika Christiana Abba

Nnamdi Azikiwe University

Felix A. Onyegbule

Nnamdi Azikiwe University

Chukwubuikem C. Okolo

Nnamdi Azikiwe University

Christoph Janiak

Heinrich Heine University Düsseldorf

Cooperative Institutions

Nnamdi Azikiwe University

Heinrich Heine University Düsseldorf

University of Nigeria

Pharmaceutical Biotechnology (Czechia)

University of Maiduguri

Ebonyi State University

Ahmadu Bello University

Michael Okpara University of Agriculture

China Three Gorges University

Chinese Academy of Sciences

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International Journal of Pharmaceutical Sciences and Research (2012)

Chinedu Fredrick Anowi Blessing Ogechukwu Umeokoli AF Onyegbule Chibuzor Onyinye Okonkwo I Chibeze

Pterocarpus santalinoides, family-Fabaceae was claimed to have analgesic properties.The people of Ogidi in Idemili North Local Government Area of Anambra State, Nigeria used it in the management of aches and pains.This study is therefore aimed at determining this claim of the activities of Pterocarpus santalinoides using the leaves which will serve as a criterion to recommend the ethno pharmacological use of the plant.The leaves of Pterocarpus santalinoides family Fabaceae were dried, powdered and extracted by cold maceration with methanol for 48hrs, it was concentrated using rotary evaporator.The analgesic activity was investigated in rats using hot plate method at a temperature of 40 o C. Phytochemical evaluation revealed the presence of tannins, flavonoids, terpenoids, steroids, alkaloids, glycosides, saponins and resins.Pterocarpus santalinoides extract (300 mg/kg) induced analgesia in rats (p< 0.05) and this effect was comparable to that of Aspirin (100 mg/kg).Acute toxicity test also revealed that the drug is safe.The claimed benefits of Pterocarpus santalinoides in traditional medical management of aches and pains could be supported by the results of this investigation.

10.13040/ijpsr.0975-8232.3(7).2018-23

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Citations (1)

Antioxidant and cytotoxicity evaluation of the Endophytic Fungus, Fusarium equiseti, isolated from the leaves of Ocimum gratissimum

Magna Scientia Advanced Research and Reviews (2024)

Onyeka Chinwuba Obidiegwu Nnamdi Markben Adione Ifeanyi Peter Onyeka Peter M. Eze Chioma Vivian Anidi

Antioxidants serve as defense mechanisms which protects against oxidative damage through preventing or retarding oxidation caused by free radicals. Free radicals are highly reactive molecules with an odd number of electrons generated from oxygen capable of damaging various cellular structures. Endophytes have shown capacity to create beneficial biologically active molecules with a wide range of pharmacological properties usually identical to those of the host plant. Their feature has continued to gain fame due to their ability to produce metabolites within the host system without causing harm to the host while sequestering reactive oxygen species within the host systems. Endophytic fungal isolation, fungal fermentation, extraction of secondary metabolites, antioxidant and cytotoxicity studies were carried out using standard laboratory methods. Therefore, this study evaluated the antioxidant and cytotoxicity activity of fusarium equiseti previously isolated from Ocimum gratissimum. The findings revealed that the crude extract of an endophyte associated with O. gratissimum called F. equiseti produced substances that have strong antioxidant and cytotoxic properties. The antioxidant and cytotoxic potential were due to presence of compounds previously reported for cytotoxic activity, such as Enniatins and Sarasinoside H1 and while the antioxidant activity was due to presence of Orientin a compound known to have shown antioxidant property. Therefore, this endophyte may serve as a source of new substances with potential for use in pharmaceutical applications for diseases associated with reactive oxygen species.

Ocimum gratissimum

10.30574/msarr.2024.10.2.0009

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Detection, isolation and identification of more bioactive compounds from Fusarium equiseti, an endophytic fungus isolated from Ocimum gratissimum

Zenodo (CERN European Organization for Nuclear Research) (2022)

Nnamdi Markben Adione Ifeanyi Peter Onyeka Chika Christiana Abba Nkeoma Nkasi Okoye Chukwubuikem C. Okolo

Ocimum gratissimum

Isolation

10.5281/zenodo.7142302

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Citations (1)

and

DOAJ (DOAJ: Directory of Open Access Journals) (2017)

Daniel Lotanna Ajaghaku O.D. Obasi Blessing Ogechukwu Umeokoli Peter Ogbuatu Chukwuemeka S. Nworu

leaves are widely used in ethnomedicine for the management of immuno-inflammatory disorders. We investigated the

EC50

Flavanone

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Citations (5)

CCDC 1946592: Experimental Crystal Structure Determination

The Cambridge Structural Database (2020)

Haiqian Yu Simon‐Patrick Höfert Mariam Moussa Christoph Janiak Wernér E.G. Müller

10.5517/ccdc.csd.cc23bl9c

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Phytochemical Screening and Atomic Absorption Spectroscopic Analysis of the Leaf and Stem Extracts of Amaranthus Spinosus

Asian Journal of Chemical Sciences (2023)

Sylvalyn Ijeoma Anyanele Vincent Ishmael Egbulefu Ajiwe Wisdom Chibuzo Anyanele Blessing Ogechukwu Umeokoli Benjamin Uchenna Modozie

This research work was carried out to determine the phytochemicals and elemental constituents present in the leaf and stem of Amaranthus spinosus. The atomic absorption spectroscopic analysis was carried out using the crushed dry plant materials while the extraction of the phytochemicals was carried out using methanol in a Soxhlet extractor. From the analysis done, the following elements were detected in the plant parts: cobalt, lead, manganese, nickel, copper, cadmium, magnesium, iron, chromium and calcium but nickel was found absent in the leaf. Some of these elements are beneficial to health. The phytochemical screening done indicated the presence of the following: alkaloids, flavonoids, saponins, tannins, steroids, volatile oils, proteins, and carbohydrates.

Phytochemical

Stem-and-leaf display

Soxhlet extractor

10.9734/ajocs/2023/v13i4244

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Screening of secondary metabolites produced by a mangroove-derived Nigrospora species an endophytic fungus isolated from Rhizophora racemosa for antioxidant and antimicrobial properties

Zenodo (CERN European Organization for Nuclear Research) (2023)

Ebube Samuel Izuogu Blessing Ogechukwu Umeokoli Onyeka Chinwuba Obidiegwu Ugochukwu Moses Okezie Chukwubuikem C. Okolo

Rhizophora mucronata

Rhizophoraceae

Medicinal fungi

Rhizophora

Marine fungi

10.5281/zenodo.8046367

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Pharmacognostic study and physiochemical assessment on the leaves of Melastomastrum capitatum Fern. anticancer plant in Nigeria

American Journal of Physiology Biochemistry and Pharmacology (2020)

Cletus Anes Ukwubile Emmanuel Ikpefan Troy Salvia Malgwi Blessing Ogechukwu Umeokoli

Background: This paper provides vital information about the pharmacognostic and physiochemical parameters of Melastomastrum capitatum leaf. The leaf has been used to treat ovarian cancer by the Fulani people in Nguroje Taraba State Nigeria. Objective: To evaluate the pharmacognostic and physiochemical parameters of M. capitatum leaf. Methods: Pharmacognostic parameters such as leaf surface data, cell inclusions and macroscopic data as well phytochemical screening of leaf extract were determined using standard procedures. Results: Phytochemical screening of n-hexane, ethyl acetate, n-butanol and aqueous fractions of leaf methanol extract of leaf showed the presence of alkaloids, flavonoids, gums and mucilage, phytosterols, carbohydrates, proteins, phytosterols and saponins with no anthracene detected. The n-hexane fraction showed only tannins and fixed oils while n-butanol and aqueous also showed the presence of most metabolites indicated in ethyl acetate fraction. Paracytic type of stomata was observed on the upper surface of leaf with a covering trichome. Prismatic calcium oxalate crystals were observed as well on the leaves. Moisture contents and extractive values ranged from 0.41 to 11.04. Conclusion: Information derived from this study will help in formulating pharmacopeia standards of drug and in taxonomic identification of the plant.

10.5455/ajpbp.20190822065441

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Survey of Plants Used in Folk Medicine in Bali, Gashaka and Sarduana Local Government Areas Taraba State Nigeria for the Treatment of Cancers

International Journal of Advanced Biological and Biomedical Research (2020)

Cletus Anes Ukwubile Jude A. Odugu Salihu Njidda Blessing Ogechukwu Umeokoli Abdulqadir Bukar Bababe

Cancer disease has been on the increase recently in both developed and developing countries of the world resulting in 40 million deaths annually. Ethno-medicinal survey of indigenous herbal plants used in traditional medicine in the treatment of cancers was conducted in major communities in Bali, Gashaka and Sarduana Local Government Areas Taraba State Nigeria. Traditional medicine practitioners as well native herbalists, herbal plants and product marketers were orally interviewed by structured questionnaires. One hundred and six (106) species of plants from forty-nine (49) families were surveyed and documented in this study. Out of these, 70 % of the plants were neither screened for their anticancer activity nor documented in literatures while no any members of the Families Salvadoraceae, Simaroubaceae, and Elatinaceae have ever been reported for their anticancer activity. Members of the Family Fabaceae have the highest number of species distribution with 15%, followed by Family Asteraceae with 9% while Family Rubiaceae and Annonaceae have 6% and 5% distribution respectively. The leaves of the plants were the most used part in the treatment of cancers in all the communities, and preparation is usually by decoction. Sides effects of these plants were generally low on observation.The need for conservation of these plants is of utmost important because most these plants might go into extinct as a result of urbanization pressure and timber activities in most of these areas surveyed.

Simaroubaceae

Local government area

10.33945/sami/ijabbr.2020.4.1

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Evaluation of Antiplasmodial and Immunomodulatory Activities of Extracts of Endophytic Fungi Isolated from four Nigerian Medicinal Plants

Planta Medica International Open (2017)

Nonye Treasure Ujam Peter M. Eze Blessing Ogechukwu Umeokoli C Abbah Festus Basden Chiedu Okoye

Endophytic fungi are the most promising source for new drugs to combat the problem of antimicrobial resistance. Twelve fungal endophytes were isolated from the leaves and stems of Azadirachta indica, Newbouldia laevis, Psidium guajava and Agerantum conyzoides using standard methods. Solid state fermentation of the isolated fungi was carried out on rice media for 21 days at 22oC and the fungal secondary metabolites were extracted using ethyl acetate. The extracts were tested for antiplasmodial activity in vivo against Plasmodium berghei (Anka strain) using Peter and Reyley's curative test methods while in vitro activity against Plasmosium falciparum was determined using standardized WHO microtest system. Immunomodulatory activity of the extract was evaluated by cyclophosphamide unduce myelosuppresion. Active extracts were further subjected to Delayed Type Hypersensitivity Response (DTHR) and Heamaglutination Inhibition Assay using SRBCs and antigens. The extracts showed varying degree of antiplasmodial activities. Growth of P.berghei was significanlty (P < 0.001) inhibited, curative effect range from 59.09 – 100%. Schizont maturation of P.falciparum isolates was inhibited and the highest level of inhibition was observed at 1 mg/ml (P < 0.05). Seven (58.33%) of the fungal extracts showed significant counteracting effect to the cyclophosphamide-induced reduction in total WBC, % neutrophil, lymphocyte and monocyte (p < 0.05). There was a significant inhibition of the DTHR and heamagglutination induced by SRBC in rats up to 78.49 and 69.23% respectively. Correlation was positive and statistically significant between malaria and Immunomodulatory activities.observed (P = 0.036, r = 0.786). HPLC-DAD analysis revealed the presence of ferulic acid, luteolin, peperine, rocaglamid, epicatechin and phenylacetic acid which have been reported to have antiplasmodial and immunomodulatory activities. Extracts of endophytic fungi isolated from these Nigerian Medicinal plants possess antiplasmodial and immunomodulatory activities which portends a novel approach for malaria therapy.

Plasmodium berghei

10.1055/s-0037-1608414

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