Wagner Luiz Tafuri

Universidade Federal de Minas Gerais

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Maria Norma Melo

Universidade Federal de Minas Gerais

Maria Marta Figueiredo

Universidade do Estado de Minas Gerais

M.S.M. Michalick

Universidade Federal de Minas Gerais

Washington Luíz Tafuri

Universidade Federal de Minas Gerais

David M. Mosser

University of Maryland, College Park

Izabela Ferreira Gontijo de Amorim

Universidade do Porto

Pedro Raso

Universidade Federal de Minas Gerais

Carolina C. Souza

Universidade Federal do Oeste da Bahia

Ricardo Gonçalves

Universidade Federal de Minas Gerais

Marta de Lana

Universidade Federal de Ouro Preto

Cooperative Institutions

Universidade Federal de Minas Gerais

289

Society for Leukocyte Biology

Institute of Immunology

Fundação Oswaldo Cruz

University of Baltimore

Federation of American Societies for Experimental Biology

Universidade Federal de Ouro Preto

Universidade de São Paulo

National Institutes of Health

Harvard University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Ultrastructural alterations of the liver in visceral leishmaniasis (Kala-azar). A study of three cases.

Revista Goiana de Medicina (1980)

Eduardo Alves Bambirra Wagner Luiz Tafuri Pedro Raso

Source

Cite

Citations (0)

American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin

PLoS ONE (2013)

Cibele Fontes Alves Cíntia F. Alves Maria Marta Figueiredo Carolina C. Souza George Luiz Lins Machado-Coelho

Background American tegumentary leishmaniasis (ATL) is endemic in Latin America, where Brazil has over 27 thousand cases per year. The aim of the present study was to develop an immunohistochemical method (IHC) for ATL diagnosis. For this purpose, we used serum from a dog naturally infected with Leishmania (L) infantum (canine hyperimmune serum) as the primary antibody, followed by a detection system with a secondary biotinylated antibody. Methodology Skin samples were obtained from 73 patients in an endemic area of Caratinga, Minas Gerais (MG) State, Brazil all testing positive for ATL with the Montenegro skin test, microscopy, and PCR. Canine hyperimmune serum of a dog naturally infected with Leishmania (L.) infantum was employed as a primary antibody in an immunohistochemical diagnostic method using streptavidin-biotin peroxidase. To assess the specificity of this reaction, IHC assays employing two monoclonal antibodies were carried out. As the polymer-based technology is less time-consuming and labor intensive than the IHC labeled streptavidin-biotin peroxidase method, we compared the two methods for all samples. Results The IHC method detected ATL in 67 of the 73 cases (91.8%). Immunolabeled parasites were primarily detected inside macrophages either in the superficial or the deep dermis. Detection was facilitated by the high contrast staining of amastigotes (dark brown) against the light blue background. A lower detection rate (71.2%) was observed with the both of the monoclonal Leishmania antibodies compared to the canine hyperimmune serum. This may have been due to a non-specific background staining observed in all histological samples rendering positive detection more difficult. The higher efficacy of the canine hyperimmune serum in the IHC method was confirmed by the method using streptavidin-biotin peroxidase as well as that with the polymer-based technology (biotin-avidin-free system). Conclusions The data are encouraging with regard to validating IHC as a standard alternative method for ATL diagnosis.

Fixative

10.1371/journal.pone.0063343

Cite

Citations (37)

Ultrastructure of the Vesicular Component in the Intramural Nervous System of the Guiena-Pig’s intestines

Zeitschrift für Naturforschung B (1964)

Wagner Luiz Tafuri

In the present paper, a systematic study of the vesicular component found in the unmyelinated nerve fibers of the intestinal nervous plexus from the serosa to the mucosa of the guinea pig was carried out. This vesicular component containing dense granules with diameters from 300 to 1100 Å was distinguished from the synaptic vesicles of 100 to 500 A. The distribution of granules and vesicles of the myenteric plexus of the duodenum and colon and extraganglion nervous fibers of the mucosa, submocusa and muscularis was studied. These results suggest that the vesicles can be the site of synthesis and storage of the substance P and 5-hydroxytryptamine.

Enteric Nervous System

Plexus

10.1515/znb-1964-0712

Cite

Citations (16)

Avaliação de diferentes fixadores na preservação das características histológicas de pele de orelha de cães

Arquivo Brasileiro de Medicina Veterinária e Zootecnia (2016)

L.S. Campos Wagner Luiz Tafuri A.J.W. Pinto

RESUMO Os fixadores biológicos desempenham um papel importante na qualidade final da histologia. Na rotina veterinária, a biópsia de pele é um procedimento comum e a escolha do fixador é primordial para resultado final adequado. Os fixadores mais usados são à base de formalina, ainda que sejam tóxicos, cancerígenos, de baixa penetração e de fixação lenta. Mesmo assim, não existe um fixador ideal que substitua as suas qualidades. O objetivo deste trabalho foi avaliar qualitativamente a preservação das características histológicas de pele de cão utilizando diferentes fixadores de tecidos incluídos em parafina, cortados e corados pela hematoxilina-eosina. Utilizou-se uma caneta Punch de 4 milímetros para coletar amostras de pele de orelha em seis cadáveres de cães. Após coleta, os tecidos foram fixados em: (1) Bouin, durante seis horas; (2) Carnoy, durante quatro horas; (3) formaldeído tamponado 10% durante 24 horas, todos sob refrigeração (4ºC). Posteriormente, os tecidos foram processados, cortados e corados em hematoxilina e eosina. As lâminas foram avaliadas, às cegas, por quatro patologistas diferentes, que consideraram aspectos qualitativos a seguir: (1) qualidade da coloração; (2) preservação das características histológicas; e (3) preservação dos limites citoplasmáticos utilizando a escala de LIKERT de pontuação para cada lâmina. O fixador com a maior média de pontuação em todos os itens foi o formol tamponado com 3,76 pontos, seguido pelo Bouin (3,39) e pelo Carnoy (2,52). O formol pode trazer riscos à saúde do profissional que rotineiramente o manuseia, portanto se faz necessária a busca por fixadores com as mesmas qualidades, mas menos nocivos à saúde.

10.1590/1678-4162-8595

Cite

Citations (3)

Expression of IFN-γ, TNF-α, IL-10 and TGF-β in lymph nodes associates with parasite load and clinical form of disease in dogs naturally infected with Leishmania (Leishmania) chagasi

Veterinary Immunology and Immunopathology (2008)

Cíntia F. Alves Izabela Ferreira Gontijo de Amorim Eliane Perlatto Moura Raul Rio Ribeiro Cibele Fontes Alves

Leishmania chagasi

Parasite load

10.1016/j.vetimm.2008.11.020

Cite

Citations (117)

Immune Complex–Driven Generation of Human Macrophages with Anti-Inflammatory and Growth-Promoting Activity

The Journal of Immunology (2020)

Elizabeth Dalby Stephen M. Christensen Jingya Wang Kajal Hamidzadeh P. Chandrasekaran

To maintain homeostasis, macrophages must be capable of assuming either an inflammatory or an anti-inflammatory phenotype. To better understand the latter, we stimulated human macrophages in vitro with TLR ligands in the presence of high-density immune complexes (IC). This combination of stimuli resulted in a broad suppression of inflammatory mediators and an upregulation of molecules involved in tissue remodeling and angiogenesis. Transcriptomic analysis of TLR stimulation in the presence of IC predicted the downstream activation of AKT and the inhibition of GSK3. Consequently, we pretreated LPS-stimulated human macrophages with small molecule inhibitors of GSK3 to partially phenocopy the regulatory effects of stimulation in the presence of IC. The upregulation of DC-STAMP and matrix metalloproteases was observed on these cells and may represent potential biomarkers for this regulatory activation state. To demonstrate the presence of these anti-inflammatory, growth-promoting macrophages in a human infectious disease, biopsies from patients with leprosy (Hanseniasis) were analyzed. The lepromatous form of this disease is characterized by hypergammaglobulinemia and defective cell-mediated immunity. Lesions in lepromatous leprosy contained macrophages with a regulatory phenotype expressing higher levels of DC-STAMP and lower levels of IL-12, relative to macrophages in tuberculoid leprosy lesions. Therefore, we propose that increased signaling by FcγR cross-linking on TLR-stimulated macrophages can paradoxically promote the resolution of inflammation and initiate processes critical to tissue growth and repair. It can also contribute to infectious disease progression.

Proinflammatory cytokine

10.4049/jimmunol.1901382

Cite

Citations (13)

Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus

Memórias do Instituto Oswaldo Cruz (2022)

Ednéia Venâncio Alves-Sobrinho Lucélia de Jesus Pinheiro Larissa Ferreira Paranaíba Igor Campos Fontes Patrícia Martins Parreiras

Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle.Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host.Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene.Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations.Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus.

Cavia

Phascolarctos cinereus

10.1590/0074-02760220065

Cite

Citations (1)

Parasitismo incomum do miocárdio e do esôfago em chagásico crônico portador de doença de Hodgkin e em uso de imunodepressores

Revista da Sociedade Brasileira de Medicina Tropical (1974)

H. O. Almeida Wagner Luiz Tafuri L Bogliolo J C Cunha

É apresentado um caso de chagásico crônico, portador de doença de Hodgkin, tratado com imunossupressores, no qual foi observado parasitismo incomum do esôfago e do miocárdio. É dada ênfase à raridade da localização esofágica de ninhos de leishmânias na doença de Chagas crônica do adulto, bem como às dimensões do parasito e às características das formas amastigotas.

10.1590/s0037-86821974000200009

Cite

Citations (12)

[On the behavior of the neurosecretory vesicular component of the mega-esophagus in human trypanosomiasis cruzi].

PubMed (1970)

Wagner Luiz Tafuri T. A. Maria

Chagas Disease

Mega-

Component (thermodynamics)

Megaesophagus

Source

Cite