Sabrina Margarucci

National Research Council

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Co-Authors

Gianfranco Peluso

Saint Camillus International University of Health and Medical Sciences

Orsolina Petillo

National Research Council

Anna Calarco

University of Palermo

Menotti Calvani

Sigma Tau (Italy)

Umberto Galderisi

University of Campania "Luigi Vanvitelli"

Mariarosa Anna Beatrice Melone

University of Campania "Luigi Vanvitelli"

Nunzio Antonio Cacciola

University of Naples Federico II

Alfonso Barbarisi

Pegaso University

V. Cosenza

Federico II University Hospital

G. Mazzacca

University of Naples Federico II

Cooperative Institutions

Trelleborg (Sweden)

University of Naples Federico II

National Research Council

Institute of Protein Biochemistry

Federico II University Hospital

University of Campania "Luigi Vanvitelli"

Institute of Biosciences and Bioresources

National Academies of Sciences, Engineering, and Medicine

University of Salerno

University of Bologna

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The genotoxicity of PEI-based nanoparticles is reduced by acetylation of polyethylenimine amines in human primary cells

Toxicology Letters (2013)

Anna Calarco Michela Bosetti Sabrina Margarucci Luca Fusaro Elena‐Raluca Nicoli

Polyethylenimine

Viability assay

Biocompatibility

PLGA

10.1016/j.toxlet.2012.12.019

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Citations (58)

Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells

Antioxidants (2020)

Francesco Di Meo Rossana Cuciniello Sabrina Margarucci Paolo Bergamo Orsolina Petillo

Oxidative stress has been associated to neuronal cell loss in neurodegenerative diseases. Neurons are post-mitotic cells that are very sensitive to oxidative stress—especially considering their limited capacity to be replaced. Therefore, reduction of oxidative stress, and inhibiting apoptosis, will potentially prevent neurodegeneration. In this study, we investigated the neuroprotective effect of Ginkgo biloba extract (EGb 761) against H2O2 induced apoptosis in SK-N-BE neuroblastoma cells. We analysed the molecular signalling pathway involved in the apoptotic cell death. H2O2 induced an increased acetylation of p53 lysine 382, a reduction in mitochondrial membrane potential, an increased BAX/Bcl-2 ratio and consequently increased Poly (ADP-ribose) polymerase (PARP) cleavage. All these effects were blocked by EGb 761 treatment. Thus, EGb 761, acting as intracellular antioxidant, protects neuroblastoma cells against activation of p53 mediated pathway and intrinsic mitochondrial apoptosis. Our results suggest that EGb 761, protecting against oxidative-stress induced apoptotic cell death, could potentially be used as nutraceutical for the prevention and treatment of neurodegenerative diseases.

10.3390/antiox9040279

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Citations (34)

Experimental colitis: decreased Octn2 and Atb0+ expression in rat colonocytes induces carnitine depletion that is reversible by carnitine‐loaded liposomes

The FASEB Journal (2006)

Giuseppe D’Argenio Menotti Calvani Amelia Casamassimi Orsolina Petillo Sabrina Margarucci

Carnitine transporters have recently been implicated in susceptibility to inflammatory bowel disease (IBD). Because carnitine is required for beta-oxidation, it was suggested that decreased carnitine transporters, and hence reduced carnitine uptake, could lead to impaired fatty acid oxidation in intestinal epithelial cells, and to cell injury. We investigated this issue by examining the expression of the carnitine transporters OCTN2 and ATB0+, and butyrate metabolism in colonocytes in a rat model of IBD induced by trinitrobenzene sulfonic acid (TNBS). We found that Octn2 and Atb0+ expression was decreased in inflammatory samples at translational and functional level. Butyrate oxidation, evaluated based on CO2 production and acetyl-coenzyme A synthesis, was deranged in colonocytes from TNBS-treated rats. Treatment with carnitine-loaded liposomes corrected the butyrate metabolic alterations in vitro and reduced the severity of colitis in vivo. These results suggest that carnitine depletion in colonocytes is associated with the inability of mitochondria to maintain normal butyrate beta-oxidation. Our data indicate that carnitine is a rate-limiting factor for the maintenance of physiological butyrate oxidation in colonic cells. This hypothesis could also explain the contradictory therapeutic efficacy of butyrate supplementation observed in clinical trials of IBD.

Enterocyte

Coenzyme A

10.1096/fj.06-5950fje

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Citations (78)

Carnitine levels in colon, muscle and serum of rats with experimental colitis

Gastroenterology (2000)

Giuseppe D’Argenio V. Cosenza Nicola Delia Valle Orsolina Petillo Sabrina Margarucci

10.1016/s0016-5085(00)81215-9

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Citations (0)

Deregulation of Mitochondrial Apoptosis in Cancer

Gianfranco Peluso Orsolina Petillo Sabrina Margarucci Anna Calarco Maura Calvani

Pathogenesis

10.1007/978-2-8178-0929-8_7

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Modulation of in vitro myogenesis induced by different polymer substrates.

Journal of Materials Science Materials in Medicine (1999)

Orsolina Petillo Sabrina Margarucci Gianfranco Peluso Alfonso Barbarisi Mariarosa Anna Beatrice Melone

C2C12

Creatine kinase

10.1023/a:1008998604276

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Citations (6)

Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects

Molecules (2019)

Nunzio Antonio Cacciola Giuseppe Squillaci Mariella D’Apolito Orsolina Petillo Francesco Veraldi

In this study, chestnut shells (CS) were used in order to obtain bioactive compounds through different extraction procedures. The aqueous extracts were chemically characterized. The highest extraction yield and total phenolic content was obtained by conventional liquid extraction (CLE). Gallic and protocatechuic acids were the main simple phenols in the extract, with 86.97 and 11.20 mg/g chestnut shells dry extract (CSDE), respectively. Six tumor cell lines (DU 145, PC-3, LNCaP, MDA-MB-231, MCF-7, and HepG2) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of CSDE (1-100 µg/mL) for 24 h, and cell viability was evaluated using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. A reduced rate in cell viability was observed in DU 145, PC-3, LNCaP, and MCF-7 cells, while viability of the other assessed cells was not affected, except for PNT2 cells at a concentration of 100 μg/mL. Furthermore, CSDE-at concentrations of 55.5 and 100 µg/mL-lead to a significant increase of apoptotic cells in DU 145 cells of 28.2% and 61%, respectively. In conclusion, these outcomes suggested that CS might be used for the extraction of several polyphenols that may represent good candidates for alternative therapies or in combination with current chemotherapeutics.

Viability assay

10.3390/molecules24183401

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Citations (38)

Mutant huntingtin regulates EGF receptor fate in non-neuronal cells lacking wild-type protein

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (2012)

Mariarosa Anna Beatrice Melone Anna Calarco Orsolina Petillo Sabrina Margarucci Luca Colucci-D’Amato

10.1016/j.bbadis.2012.09.001

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Citations (17)

Marine-Derived Polysaccharide Hydrogels as Delivery Platforms for Natural Bioactive Compounds

International Journal of Molecular Sciences (2025)

Fabrizia Sepe Anna Valentino Loredana Marcolongo Orsolina Petillo Raffaele Conte

Marine polysaccharide hydrogels have emerged as an innovative platform for regulating the in vivo release of natural bioactive compounds for medical purposes. These hydrogels, which have exceptional biocompatibility, biodegradability, and high water absorption capacity, create effective matrices for encapsulating different bioactive molecules. In addition, by modifying the physical and chemical properties of marine hydrogels, including cross-linking density, swelling behavior, and response to external stimuli like pH, temperature, or ionic strength, the release profile of encapsulated bioactive compounds is strictly regulated, thus maximizing therapeutic efficacy and minimizing side effects. Finally, by using naturally sourced polysaccharides in hydrogel formulations, sustainability is promoted by reducing dependence on synthetic polymers, meeting the growing demand for eco-friendly materials. This review analyzes the interaction between marine polysaccharide hydrogels and encapsulating compounds and offers examples of how bioactive molecules can be encapsulated, released, and stabilized.

Biocompatibility

Natural polymers

10.3390/ijms26020764

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Functional Plant-Based Beverage Fortified with Hazelnut Cuticle Polyphenols: Antioxidant and Phenolic Content Characterization

Molecules (2025)

Raffaele Conte Fabrizia Sepe Sabrina Margarucci Eleonora Costanzo Orsolina Petillo

In recent decades, there has been growing interest in the fortification of food products with antioxidants and phenolics derived from plant by-products. The present study focused on the production of a plant-based beverage enriched with hazelnut cuticle extract to characterize its antioxidant content, phenolic profile, and organoleptic characteristics. Liquid chromatography-mass spectrometry (LC-MS) enabled the identification of key polyphenols in hazelnut cuticles, including catechin, epicatechin, and quercetin derivatives, guiding the selection of a biocompatible Natural Deep Eutectic Solvent (NADES) composed of choline chloride and lactic acid for efficient extraction. The obtained phytochemical profile of the extract revealed a high concentration of bioactive compounds, with a Total Phenolic Content of 160.88 ± 14.27 mg GAE/g and Antioxidant Power measured by DPPH of 5848.2 ± 11.3 μmol TE/g. The bioaccessibility of phenolics in the fortified hazelnut-based beverage was determined after in vitro digestion, reaching a value of 89.7%, indicating excellent release and stability during digestion. Organoleptic evaluation revealed high sensory acceptability, with aftertaste scoring 3.61 ± 0.4 respect the 3.94 ± 1.3 result of reference milk, on a 5-point scale. In conclusion, this study demonstrates the potential for sustainable valorization of hazelnut cuticles, through their incorporation as NADES extracts in plant-based milk, providing an innovative solution to reduce food waste while catering to consumer demand for nutritionally enriched and eco-friendly products.

Organoleptic

Phytochemical

10.3390/molecules30030433

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