Abstract Cytophotometric analyses of RNA and acetylcholinesterase responses of caudate and cerebrocortical neurons of soman toxicated rats were conducted to characterize impairments in regulatory aspects of neuronal metabolism occurring in the acute phase of cholinesterase impairment. There was a severe and dose‐dependent suppression (20–60%) in neuronal acetylcholinesterase activity in both a.m. and p.m.‐treated rats; no diurnal differences were apparent in control acetylcholinesterase levels or neuronal acetylcholinesterase responsiveness to soman toxication. RNA levels, however, were markedly higher in p.m. than in a.m. saline‐treated controls. Soman depressed caudate neuron RNA contents in the afternoon, but not in the morning. Cerebrocortical neuron RNA levels were suppressed in both a.m. and p.m.‐toxicated rats, although this RNA depletion was more severe in the afternoon. These results indicate that soman can elicit marked alterations in neuronal transcriptional‐translational capabilities and that there are diurnal variations in cellular metabolic responsiveness to soman toxication. Although functional relationships between soman‐induced cholinesterase inhibition and RNA depletion remain to be elucidated, depressed RNA metabolism appears to be a maladaptive response preventing rapid regeneration of cholinesterase following poisoning.

Cholinesterase

Acetylcholinesterase inhibitor

10.1002/cbf.290010106

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Citations (19)

Soman intoxication in hypothyroid rats: alterations in brain neuronal RNA, acetylcholinesterase and survival

Neurochemistry International (1986)

J. Swisher Jeffrey A. Doebler Adam Anthony

Cholinesterase

10.1016/0197-0186(86)90096-3

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Citations (4)

Effects of chlorpromazine on soman induced brain neuronal rna depletion and lethality in rats

Federation Proceedings (1985)

Thomas J. Wall J. Swisher Jeffrey A. Doebler Adam Anthony

Lethality

Source

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Citations (0)

Effects of neutral ionophores on membrane electrical characteristics of NG108-15 cells

Toxicology Letters (2000)

Jeffrey A. Doebler

Valinomycin

Hyperpolarization

Protonophore

Nigericin

10.1016/s0378-4274(99)00193-9

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Citations (32)

Quantitative cytochemical analyses of ovarian luteal and interstitial cell RNA in relation to plasma progestins during pseudopregnancy in the rat

Cell Biochemistry and Function (1983)

Jeffrey A. Doebler Edward W. Wickersham Adam Anthony

Abstract Luteal and interstial cell RNA contents and circulating progesterone (P) and 20α‐hydroxypregn‐4‐ene‐3‐one (20α‐OH P) levels were measured during pseudopregnancy in order to characterize relationships between ovarian 20α‐OH P secretion and luteal regression. Functional luteolysis, as manifested in depressed P levels, was not associated with concurrent elevations in 20α‐OH P. Rather, augmented 20α‐OH P levels were evidenced in periovulatory periods at the onset and termination of pseudopregnancy, subsequent to RNA accumulation in both luteal and interstitial compartments. It is postulated that 20α‐OH P, the putative inactive metabolite of P, is produced by both ovarian compartments in a cyclic manner and in response to gonadotrophin released in the preovulatory period.

Luteolysis

Interstitial cell

10.1002/cbf.290010310

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Citations (2)

Adrenocortical metabolism and plasma corticosterone in soman intoxicated rabbits

Experientia (1985)

Jeffrey A. Doebler Edward W. Wickersham L. V. Polakovic Tsung‐Ming Shih Adam Anthony

Corticosterone

Dose

Cholinesterase

Neurotoxin

10.1007/bf01951700

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Citations (2)