Kaiyu Wang

Hiroshima University

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Yi Geng

Sichuan Agricultural University

Yan Gao

Wuhan Polytechnic University

Yang He

China Agricultural University

D F Chen

Sichuan Agricultural University

Xingmei Duan

Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

Ke Men

Sichuan University

Junge Wang

Sichuan Agricultural University

Masaru Ueno

Hiroshima University

Xizi Fu

Sichuan University

Lishi Huang

Zhejiang Sci-Tech University

Cooperative Institutions

Sichuan University

Sichuan Agricultural University

State Key Laboratory of Biotherapy

University of Electronic Science and Technology of China

Pratt Institute

Johns Hopkins University

Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

Sun Yat-sen University

West China Hospital of Sichuan University

Kumamoto University

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Safety and immunogenicity of an oral DNA vaccine encoding Sip of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus delivered by live attenuated Salmonella typhimurium

Fish & Shellfish Immunology (2014)

Lishi Huang Kaiyu Wang Dan Xiao D F Chen Yi Geng

Aroa

10.1016/j.fsi.2014.02.017

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Citations (77)

Dual-mRNA Delivery Using Tumor Cell Lysate-Based Multifunctional Nanoparticles as an Efficient Colon Cancer Immunogene Therapy

International Journal of Nanomedicine (2024)

Kaiyu Wang Yan Gao Shan Wu Jin Zhang Manfang Zhu

Background: Messenger RNA (mRNA)-based immunogene therapy holds significant promise as an emerging tumor therapy approach. However, the delivery efficiency of existing mRNA methods and their effectiveness in stimulating anti-tumor immune responses require further enhancement. Tumor cell lysates containing tumor-specific antigens and biomarkers can trigger a stronger immune response to tumors. In addition, strategies involving multiple gene therapies offer potential optimization paths for tumor gene treatments. Methods: Based on the previously developed ideal mRNA delivery system called DOTAP-mPEG-PCL (DMP), which was formed through the self-assembly of 1.2-dioleoyl-3-trimethylammonium-propane (DOTAP) and methoxypoly (ethylene glycol)-b-poly (ϵ-caprolactone) (mPEG-PCL), we introduced a fused cell-penetrating peptide (fCPP) into the framework and encapsulated tumor cell lysates to form a novel nanovector, termed CLSV system (CLS: CT26 tumor cell lysate, V: nanovector). This system served a dual purpose of facilitating the delivery of two mRNAs and enhancing tumor immunogene therapy through tumor cell lysates. Results: The synthesized CLSV system had an average size of 241.17 nm and a potential of 39.53 mV. The CLSV system could not only encapsulate tumor cell lysates, but also deliver two mRNAs to tumor cells simultaneously, with a transfection efficiency of up to 60%. The CLSV system effectively activated the immune system such as dendritic cells to mature and activate, leading to an anti-tumor immune response. By loading Bim-encoded mRNA and IL-23A-encoded mRNA, CLSV/Bim and CLSV/IL-23A complexes were formed, respectively, to further induce apoptosis and anti-tumor immunity. The prepared CLSV/dual-mRNA complex showed significant anti-cancer effects in multiple CT26 mouse models. Conclusion: Our results suggest that the prepared CLSV system is an ideal delivery system for dual-mRNA immunogene therapy. Keywords: mRNA gene therapy, dual-mRNA delivery, tumor cell lysates, Bim and IL-23A mRNAs, immune response

Cancer Therapy

10.2147/ijn.s452548

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Efficient Colon Cancer Immunogene Therapy Through Co-Delivery of IL-22BP mRNA and Tumor Cell Lysate by CLSV Nanoparticles

International Journal of Nanomedicine (2023)

Jing Huang Kaiyu Wang Xizi Fu Manfang Zhu Xiaohua Chen

Background: Messenger ribonucleic acid (mRNA)-based gene therapy has great potential in cancer treatment. However, the application of mRNA-based cancer treatment could be further developed. Elevated delivery ability and enhanced immune response are advantages for expanding the application of mRNA-based cancer therapy. It is crucial that the prepared carrier can cause an immune reaction based on the efficient delivery of mRNA. Methods: We reported DMP nanoparticle previously, which was obtained by the self-assembly of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and (ethylene glycol)- b -poly (ϵ-caprolactone) (mPEG-PCL). Research demonstrated that DMP can deliver mRNA, siRNA, and plasmid. And it is applied to various tumor types. In our work, the tumor cell lysate was introduced to the internal DMP chain, fusing cell-penetrating peptides (CPPs) modification on the surface forming the CLSV system. And then mixed encoded IL-22BP (interleukin-22 binding protein) mRNA and CLSV to form CLSV/IL-22BP complex. Results: The size of the CLSV system was 213.2 nm, and the potential was 45.7 mV. The transfection efficiency of the CLSV system is up to 76.45% in C26 cells via the micropinocytosis pathway. The CLSV system also could induce an immune response and significantly elevate the expression of CD80, CD86, and MHC-II in vivo. Then, by binding with IL-22BP (Interleukin-22 binding protein) mRNA, the CLSV/IL-22BP complex inhibited tumor cell growth, with an inhibition rate of up to 82.3% in vitro. The CLSV/IL-22BP complex also inhibited tumor growth in vivo, the tumor cell growth inhibition up to 75.0% in the subcutaneous tumor model, and 84.9% in the abdominal cavity metastasis tumor model. Conclusion: Our work demonstrates that the CLSV system represents a potent potential for mRNA delivery. Keywords: mRNA, cell-penetrating peptide, gene therapy, tumor cell lysate, immune response

CD80

10.2147/ijn.s439381

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The pathology of selenium deficiency in Cyprinus carpio L

Journal of Fish Diseases (2013)

Kaiyu Wang Chunlin Peng J L Huang Yong Huang Minxia Jin

Abstract Selenium is essential for the normal life processes, and all animals, including fish, need this inorganic element. In order to research the pathology of selenium deficiency in common carp, C yprinus carpio L ., 360 juvenile carp were allocated to three treatments. Each treatment containing 120 fish was randomly divided into four groups, fed with purified diets containing selenium at 0, 0.15, 0.30 and 0.45 mg kg −1 . The results indicated that the morbidity and mortality rates of the test groups were negatively correlated with the level of selenium in the diets. The morbidity and mortality rates were, respectively, 46.7%, 33.3%, 13.3%, 0 and 26.7% 16.7%, 6.7%, 0. The affected fish exhibited pathological changes, such as ‘thin back disease’ and lordosis. Histologically, there was no abnormality observed in muscles, liver and pancreas. Ultrastructurally, there was swelling of the mitochondria of the cardiac muscle cells, the liver cells and renal tubular epithelial cells, with disintegration and lysis of the cristae of the mitochondria which vesiculated. Serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels were increased and the activity of the serum glutathione peroxidase and the serum superoxide dismutase was decreased; in contrast, the serum malonaldehyde concentration was increased.

Selenium deficiency

Transaminase

10.1111/jfd.12030

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Decreased mitochondrial translation confers 3,3'-Diindolylmethane resistance to Schizosaccharomyces pombe

Biochemical and Biophysical Research Communications (2024)

Kaiyu Wang H. Nagai Samiul Alam Rajib Yorifumi Satou Masaru Ueno

Schizosaccharomyces

10.1016/j.bbrc.2024.150864

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The losses of Lem2 and Bqt4 exhibit similar impacts on intracellular movement dynamics in fission yeast.

Biochemical and Biophysical Research Communications (2025)

Kaiyu Wang Masaru Ueno

Dynamics

10.1016/j.bbrc.2025.151326

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Identification of the major mannose-binding proteins from chicken egg yolk and chicken serum as immunoglobulins.

Proceedings of the National Academy of Sciences (1986)

Kaiyu Wang Craig A. Hoppe Pradip K. Datta A Fogelstrom Y C Lee

Chicken egg yolk contains a mannose-binding protein that could be purified with a modification of the procedure of affinity chromatography and gel filtration used for chicken serum mannose-binding protein. The yolk protein was indistinguishable from the serum protein with respect to apparent molecular masses (169 +/- 7 kDa), subunits (74 kDa and 27 kDa, in approximately 1:1 ratio) produced after denaturation in the presence of mercaptoethanol, immunoreactivity with antibody against the chicken serum mannose-binding protein, amino acid composition, pH optimum for binding, calcium independence of binding, sugar-binding specificity, and specific-binding activity. Moreover, the chicken mannose-binding proteins cross-reacted with gamma-chain-specific antibody against chicken IgG. The binding proteins were identified as IgGs by several other criteria: identical electrophoresis pattern when subjected to reducing and nonreducing NaDodSO4/polyacrylamide gel electrophoresis, cochromatography on a Fractogel TSK HW-55(F) column, similar amino acid composition, and isolation of mannose-binding protein from purified serum IgG at a yield comparable to whole serum. These results support the notion that the mannose-binding proteins from the chicken serum and egg yolk are similar, if not identical, and are a subset of chicken IgG.

Yolk

10.1073/pnas.83.24.9670

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Molecular cloning and bioinformatic analysis of the Streptococcus agalactiae neuA gene isolated from tilapia

Genetics and Molecular Research (2015)

E L Wang Kaiyu Wang D F Chen Yi Geng Lishi Huang

Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. To study the structure and function of the S. agalactiae neuA gene, we isolated it from diseased tilapia, amplified it using polymerase chain reaction (PCR) with specific primers, and cloned it into a pMD19-T vector. The recombinant plasmid was confirmed by PCR and restriction enzyme digestion, and identified by sequencing. Molecular characterization analyses of the neuA nucleotide amino acid sequence were performed using bioinformatic tools and an online server. The results showed that the neuA nucleotide sequence contained a complete coding region, which comprised 1242 bp, encoding 413 amino acids (aa). The aa sequence was highly conserved and contained a Glyco_tranf_GTA_type superfamily and an SGNH_hydrolase superfamily conserved domain, which are related to sialic acid activation catalysis. The NeuA protein possessed many important sites related to post-translational modification, including 28 potential phosphorylation sites and 2 potential N-glycosylation sites, had no signal peptides or transmembrane regions, and was predicted to reside in the cytoplasm. Moreover, the protein had some B-cell epitopes, which suggests its potential in development of a vaccine against S. agalactiae infection. The codon usage frequency of neuA differed greatly in Escherichia coli and Homo sapiens genes, and neuA may be more efficiently expressed in eukaryotes (yeast). S. agalactiae neuA from tilapia maintains high structural homology and sequence identity with CMP-NeuNAc synthetases from other bacteria.

10.4238/2015.june.1.18

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Citations (5)