Bushuai Zhong

Nanjing Agricultural University

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Feng Wang

Nanjing Agricultural University

Yanli Zhang

Nanjing Agricultural University

Yongjie Wan

Nanjing Agricultural University

Ruoxin Jia

East China Normal University

Yixuan Fan

Nanjing Agricultural University

Jihao You

University of Guelph

Ziyu Wang

Nanjing Agricultural University

Zhengrong Zhou

Shantou University

S.J. Ying

Nanjing Agricultural University

Yibo Yan

Sichuan Research Center of New Materials

Cooperative Institutions

Nanjing Agricultural University

Central South University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Nanjing Medical University

Ministry of Agriculture and Rural Affairs

Zhejiang University

Jilin University

Fudan University

Air Force Medical University

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Production of myostatin-targeted goat by nuclear transfer from cultured adult somatic cells

Theriogenology (2012)

Zhengrong Zhou Bushuai Zhong Ruoxin Jia Yongjie Wan Yanli Zhang

Myostatin

10.1016/j.theriogenology.2012.08.006

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Citations (21)

Growth regulation, imprinting, and epigenetic transcription-related gene expression differs in lung of deceased transgenic cloned and normal goats

Theriogenology (2013)

Meng Li Ruoxin Jia Yanyan Sun Zi-Yu Wang Yongjie Wan

Genomic Imprinting

10.1016/j.theriogenology.2013.10.023

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Citations (9)

[Repairing peripheral nerve defects by tissue engineering techniques:an experimental study].

PubMed (2000)

Cui Dai Qingqing Shang W Wang Yilin Cao Jianghui Dong

To develop a novel effective substitute material or technique to repair peripheral nerve gap.We inoculated expanded Schwann cells (SCs) at re-arranged bio-absorbable polymer polyglycolic acid (PGA) fiber and incubated for two weeks, then we developed a novel tissue-engineered scaffolds. The scaffolds were used as cellular isografts to bridge 15 mm long gap of sciatic nerve in inbred strains of Wistar rats. In an autologous and pure PGA fiber control group, the same surgical procedure was used. Evaluation included general observation, electromyographic examination, muscle measurement, and histological observation of serial sections at 12 weeks after surgery. The total number and density of reinnervation and thickness of myelin sheath was measured by computerized image analysis.SCs put out a long and thin prominence and migrated along the PGA fibers in spirality or parallel when they divided and finally rank into a cell-chain formation similar to Büngner's band. TEM/SEM and immunohistochemical survey demonstrated that the SCs at PGA fiber also secreted a great deal of ECMs included laminin, which play a very important role in peripheral nerve regeneration. Non-tubular scaffold comprised SCs and laminin (LN) channel in three-dimensional longitudinal rank. Animal transplantation study indicated the sensory and motor functional results of hindlimbs of experimental group rats reached to similar level of those found in nerve autograft control group. The number of reinnervation in the experimental group rats was slightly fewer than that in nerve autograft control group; but the axonal density was just reversal between the two groups because of a large area ischemic necrosis in the center of nerve autografts.This new paradigm offers a potential solution to repairing a long gap of peripheral nerve.

Reinnervation

Nerve fiber

Schwann cell

Bridge (graph theory)

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Citations (1)

Hepatoprotective Effect of an Immortal Human Fetal Hepatic Cell Transplantation on CCL4-Induced Acute Liver Injury in Mice

Transplantation Proceedings (2010)

Yaping Yan Hu Song Bushuai Zhong Z.Y. Wang S.J. Ying

CCL4

10.1016/j.transproceed.2010.04.066

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Citations (4)

Isolation, characterization, and gene modification of dairy goat mesenchymal stem cells from bone marrow

In Vitro Cellular & Developmental Biology - Animal (2012)

Yanli Zhang Yixuan Fan Ziyu Wang Yongjie Wan Zhengrong Zhou

Homeobox protein NANOG

CD90

10.1007/s11626-012-9530-z

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Citations (9)

Production of dairy goat embryos, by nuclear transfer, transgenic for human acid β-glucosidase

Theriogenology (2010)

Y.L. Zhang Yongjie Wan Z.Y. Wang Dachao Xu Xinzhi Pang

Transgenesis

10.1016/j.theriogenology.2009.11.008

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Citations (31)

MicroRNA-Mediated Myostatin Silencing in Caprine Fetal Fibroblasts

PLoS ONE (2014)

Bushuai Zhong Yanli Zhang Yibo Yan Ziyu Wang Shijia Ying

Myostatin functions as a negative regulator of skeletal muscle growth by suppressing proliferation and differentiation of myoblasts. Dysfunction of the myostatin gene, either due to natural mutation or genetic manipulations such as knockout or knockdown, has been reported to increase muscle mass in mammalian species. RNA interference (RNAi) mediated by microRNAs (miRNAs) is a promising method for gene knockdown studies. In the present study, transient and stable silencing of the myostatin gene in caprine fetal fibroblasts (CFF) was evaluated using the two most effective constructs selected from four different miRNA expression constructs screened in 293FT cells. Using these two miRNA constructs, we achieved up to 84% silencing of myostatin mRNA in transiently transfected CFF cells and up to 31% silencing in stably transfected CFF cells. Moreover, off-target effects due to induction of interferon (IFN) response genes, such as interferon beta (IFN-β) and 2′-5′-oligoadenylate synthetase 2 (OAS2), were markedly fewer in stably transfected CFF cells than in transiently transfected cells. Stable expression of anti-myostatin miRNA with minimal induction of interferon shows great promise for increasing muscle mass in transgenic goats.

Myostatin

10.1371/journal.pone.0107071

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Citations (5)

Scd1 mammary-specific vector constructed and overexpressed in goat fibroblast cells resulting in an increase of palmitoleic acid and oleic acid

Biochemical and Biophysical Research Communications (2013)

Lizhong Wang Jihao You Bushuai Zhong Caifang Ren Yanli Zhang

Palmitoleic acid

10.1016/j.bbrc.2013.11.091

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Citations (12)

Effects of different dietary intake on mRNA levels of MSTN, IGF-I, and IGF-II in the keletal muscle of Dorper and Hu sheep hybrid F1 rams

Genetics and Molecular Research (2014)

Hui Xing Z.Y. Wang Bushuai Zhong S.J. Ying Haitao Nie

MSTN, IGF-І (insulin-like growth factor-І) and IGF-II (insulin-like growth factor-II) regulate skeletal muscle growth.This study investigated the effects of different dietary intake levels on skeletal muscles.Sheep was randomly assigned to 3 feeding groups: 1) the maintenance diet (M), 2) 1.4 x the maintenance diet (1.4M), and 3) 2.15 x the maintenance diet (2.15M).Before slaughtering the animals, blood samples were collected to measure plasma urea, growth hormone, and insulin concentrations.After slaughtering, the longissimus dorsi, semitendinosus, semimembranosus, gastrocnemius, soleus, and chest muscle were removed to record various parameters, including the mRNA expression levels of MSTN and IGFs, in addition to skeletal muscle fiber diameter and cross-sectional area.The result showed that as dietary intake improved, the mRNA expression levels ©FUNPEC-RP www.funpecrp.com.br

10.4238/2014.july.24.4

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Citations (8)

Overexpression of STRA8, BOULE, and DAZL Genes Promotes Goat Bone Marrow-Derived Mesenchymal Stem Cells In Vitro Transdifferentiation Toward Putative Male Germ Cells

Reproductive Sciences (2016)

Peizhen Li Guangyao Yan Le Han Jing Pang Bushuai Zhong

Transdifferentiation

10.1177/1933719116654990

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Citations (29)