Journal Article Tuber-forming Substances in Jerusalem Artichoke (Helianthus tuberosus L.) Get access Hideyuki Matsuura, Hideyuki Matsuura Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Teruhiko Yoshihara, Teruhiko Yoshihara Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Author to whom correspondence should be addressed. Search for other works by this author on: Oxford Academic Google Scholar Akitami Ichihara, Akitami Ichihara Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Yoshio Kikuta, Yoshio Kikuta Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, JapanDepartment of Botany, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Yasunori Koda Yasunori Koda Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, JapanDepartment of Botany, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 57, Issue 8, 1 January 1993, Pages 1253–1256, https://doi.org/10.1271/bbb.57.1253 Published: 01 January 1993 Article history Received: 25 December 1992 Published: 01 January 1993
Jasmonic acid (JA) is a plant hormone that plays important roles in a large number of processes in stress adaptation and development in flowering plants. A search of genome database indicated the existence of allene oxide synthase (AOS), an enzyme of JA biosynthesis, in Physcomitrella patens, a model plant among mosses. In this study, the presence of JA was detected in P. patens. The recombinant AOS of P. patens, which was overexpressed in Escherichia coli, showed AOS activity. These data suggest that the octadecanoid pathway also exists in P. patens.
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12-Oxo-phytodienoic acid (OPDA) is an important metabolite on the octadecanoid pathway of plants. This study shows an efficient in vitro synthesis of (+)-cis-OPDA by using a flaxseed extract and an allene oxide cyclase. The OPDA yield of the reaction in this study was almost 7-fold higher than that in the conventional reaction with the flaxseed extract.
A novel 5-hydroxymethyl-2-furfural (HMF; 1) derivative, which is named asfural (compound 2), was isolated from enzyme-treated asparagus extract (ETAS) along with HMF (1) as a heat shock protein 70 (HSP70) inducible compound. The structure of compound 2 was elucidated on the basis of its spectroscopic data from HREIMS and NMR, whereas the absolute configuration was determined using chiral HPLC analysis, compared to two synthesized compounds, (S)- and (R)-asfural. As a result, compound 2 derived from ETAS was assigned as (S)-(2-formylfuran-5-yl)methyl 5-oxopyrrolidine-2-carboxylate. When compound 2, synthesized (S)- and (R)-asfural, and HMF (1) were evaluated in terms of HSP70 mRNA expression-enhancing activity in HL-60 cells, compound 2 and (S)-asfural significantly increased the expression level in a concentration-dependent manner. HMF (1) also showed significant activity at 0.25 mg/mL.
α-1,4-Glucan lyases [glycoside hydrolase family (GH) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (AF), while GH31 α-glucosidases normally catalyze a hydrolytic reaction. We determined that a small amount of AF was produced by GH31 Aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate.
