V.V. Kulguskin

Russian Academy of Sciences

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Yu. V. Ilyin

Engelhardt Institute of Molecular Biology

E. M. Lukanidin

Danish Cancer Society

G. P. Georgiev

Institute of Gene Biology

N. V. Lyubomirskaya

Russian Academy of Sciences

G P Georgiev

Engelhardt Institute of Molecular Biology

V.G. Chmeliauskate

Russian Academy of Sciences

E. V. Ananiev

Pioneer Hi-Bred

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N. А. Tchurikov

Engelhardt Institute of Molecular Biology

A.A. Bayev

Engelhardt Institute of Molecular Biology

G.P. Georgjev

Cooperative Institutions

Russian Academy of Sciences

Institute of Molecular Genetics

Engelhardt Institute of Molecular Biology

Institute of Gene Biology

Research Institute of Medical Genetics of Russian Academy of Medical Sciences

Lomonosov Moscow State University

Institute of Bioorganic Chemistry

Danish Cancer Society

Vavilov Institute of General Genetics

Institute of Cell Biophysics

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Studies on dissociation and reconstitution of nuclear 30-S ribonucleoprotein particles containing pre-mRNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis (1980)

V.V. Kulguskin A. A. Krichevskaya E. M. Lukanidin Georgii P. Georgiev

Ribonucleoprotein particle

Messenger RNP

Precursor mRNA

10.1016/0005-2787(80)90115-x

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Citations (9)

Mobile dispersed genetic element MDGI of Drosophila melanogaster: structural organization

Nucleic Acids Research (1980)

Yu. V. Ilyin V.G. Chmeliauskate E. V. Ananiev N. V. Lyubomirskaya V.V. Kulguskin

The whole-length mobile dispersed genetic element mdg1 has been cloned from D. melanogaster genome. It contains DNA fragments described earlier as Dm225 and Dm234, Mdg1 is 7.2 kb long and framed with two direct repeats of 300-400 base pairs each. Mdg1 family is represented by about 25 copies in the genome of flies and by 200 copies in the genome of cultured cell line 67J25D. Virtually all the copies in the genome of D. melanogaster have the same restriction map. Oligo(dA)-oligo(dT) regions were found within mdg1.

Melanogaster

Retrotransposon

Repeated sequence

DNA Transposable Elements

Genomic Organization

Restriction map

10.1093/nar/8.22.5333

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Citations (24)

Free Informofers and Reconstitution of 30S RNP

Cold Spring Harbor Symposia on Quantitative Biology (1978)

V.V. Kulguskin E. M. Lukanidin G P Georgiev

As early as 1968 it was proposed that the nuclear ribonucleoprotein (RNP) complexes containing pre-mRNA possess the beads-on-a-string structure with pre-mRNA wrapped on the surface of the globular protein particles which we call "informofers" (Samarina et al. 1968). The existence of a repeating structural unit was proved directly (Samarina et al. 1968), whereas only some indirect evidence in favor of surface localization of the RNA in the particle was obtained (Georgiev and Samarina 1971). In this paper we present some data confirming the latter statement (these data were obtained by V. V. Kulguskin and E. M. Lukanidin in our laboratory).

30S

10.1101/sqb.1978.042.01.092

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General Properties of Mobile Dispersed Genetic Elements in Drosophila melanogaster

Cold Spring Harbor Symposia on Quantitative Biology (1981)

N. А. Tchurikov Yu. V. Ilyin K. G. Skryabin E. V. Ananiev A.A. Bayev

Movable genetic elements in the genomes of eukaryotes have been known since the early 1950s (McClintock 1952). However, studies on their molecular organization could be started only after the invention of recombinant DNA techniques. Finnegan et al. (1978) and Ilyin et al. (1978) reported the cloning of several DNA sequences characterized by the following properties: (1) They were responsible for the synthesis of a large amount of cellular poly(A)+ RNA, (2) they were represented by a high number of copies per genome, and (3) they were scattered throughout the whole genome, occurring in different chromosomes at many different sites.

Cloning (programming)

10.1101/sqb.1981.045.01.083

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Citations (67)

The isolation of informofers free from dRNA

FEBS Letters (1971)

E. M. Lukanidin N. А. Аitkhozhinа V.V. Kulguskin G. P. Georgiev

Biological sciences

10.1016/0014-5793(71)80488-x

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Citations (9)

Mobile dispersed genetic element MDGI of Drosophila melanogaster: transcription pattern

Nucleic Acids Research (1980)

Yu. V. Ilyin V.G. Chmeliauskate V.V. Kulguskin G. P. Georgiev

Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-length transcript represented by˜ 2 poly(A)+RNA. Besides it,˜15S poly(A)+RNA is present in the cytoplasm of culture cells which corresponds to the 3′-part of 29S RNA and possibly to a small region near the 5′-end of the latter. The major fraction of mdg1 transcripts is present in the fraction of free cytoplasmic RNP particles while only 10–20% are recovered in polysomes. In this respect, mdg1 is similar to mdg3 and quite different from two single-copy genes transcripts of which are almost completely recovered in polysomes. The relationship between mobile dispersed genetic elements and endogeneous viral genomes is discussed.

DNA Transposable Elements

Transcription

10.1093/nar/8.22.5347

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Citations (16)

Mobile dispersed genetic element MDG1 of Drosophila melanogaster: nucleotide sequence of long terminal repeats

Nucleic Acids Research (1981)

V.V. Kulguskin Yu. V. Ilyin G.P. Georgjev

Long terminal repeats (LTRs) of two members of mdg1 family were sequenced. In the both cases, they are represented by perfect direct repeats 442 and 444 bp in length. Sixteen nucleotides in the LTRs of two different mdg1 elements are different. Each LTR contains slightly mismatched 16-nucleotide inverted repeats located at the ends of the LTR. Six base pairs closest to the termini of LTR form perfect inverted repeats. On the gene-distal sides of LTRs, short 4-nucleotide direct repeats are located, probably representing the duplication of a target DNA sequence arising from insertion of mdg. They are different in the two cases analyzed. Just as the other analyzed eukaryotic transposable elements, mdg1 starts with TGT and ends with ACA. Within the both strands of LTR, the sequences similar to Hogness box (a putative signal for RNA initiation, or a selector) and AATAAA blocks (putative polyadenylation signals) are present. The LTR of mdg1 contains many short direct and inverted repetitive sequences. These include a 10-nucleotide sequence forming a perfect direct repeat with the first ten nucleotides of the LTR. A region of LTR about 70 bp long is represented by simple repetitive sequences (TAT).

Direct repeat

Inverted repeat

Repeated sequence

10.1093/nar/9.14.3451

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Citations (36)