Isabel Montesinos

UCLouvain

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Olivier Denis

Centre Hospitalier Universitaire Brugmann

Hector Rodríguez-Villalobos

UCLouvain

Marie‐Luce Delforge

Université Libre de Bruxelles

Sanata Bamba

Nazi Boni University

Isidore W. Yerbanga

Nazi Boni University

S Diallo

Centre Muraz

Katrien Lagrou

KU Leuven

Deborah Steensels

Université Libre de Bruxelles

A. Sierra

EOG Resources (United States)

Frédérique Jacobs

Université Libre de Bruxelles

Cooperative Institutions

Université Libre de Bruxelles

109

Erasmus Hospital

UCLouvain

Centre Hospitalier Universitaire de Saint-Pierre

KU Leuven

Cliniques Universitaires Saint-Luc

CHU Dinant Godinne UCL Namur

Queen Fabiola Children's University Hospital

Centre Hospitalier Universitaire Brugmann

Hospital Universitario de Canarias

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Trends in production of extended-spectrum -lactamases among Enterobacteriaceae of clinical interest: results of a nationwide survey in Belgian hospitals

Journal of Antimicrobial Chemotherapy (2010)

Hector Rodríguez-Villalobos Pierre Bogaerts C. Berhin Caroline Bauraing A. Deplano

to assess the frequency and diversity of extended-spectrum β-lactamases (ESBLs) in Enterobacteriaceae isolates in Belgium.during 2006 and 2008, non-duplicate clinical isolates of Enterobacteriaceae resistant to ceftazidime and/or cefotaxime were collected in 100 Belgian hospitals. ESBL production was confirmed by phenotypic and genotypic tests. MICs of 13 antimicrobial agents were determined by Etest. ESBL-encoding genes were identified by PCR sequencing and the bla(CTX-M) environment was characterized by PCR mapping. Selected isolates were genotyped by PFGE, multilocus sequence typing analysis and phylogenetic grouping by PCR.overall, 733 isolates were confirmed as ESBL producers. Carbapenems and temocillin were active against ≥ 95% of all tested isolates. Co-resistance to co-trimoxazole and to ciprofloxacin was found in almost 70% and 80% of the strains, respectively. Overall, Escherichia coli (49%), Enterobacter aerogenes (32%) and Klebsiella pneumoniae (9%) represented the most prevalent species. Isolates harboured predominantly TEM-24 (30.7%), CTX-M-15 (24.2%) and TEM-52 (12.1%). Compared with 2006, the proportion of CTX-M-type enzymes increased significantly in 2008 (54% versus 23%; P < 10(-6)), mostly linked to a rising proportion of CTX-M-15-producing E. coli. TEM-24 decreased (19% in 2008 versus 43% in 2006; P < 10(-6)) during the same period, while the prevalence of TEM-52 remained unchanged (10% in 2008 versus 14% in 2006; not significant). Over 80% of the CTX-M-15-producing E. coli isolates clustered into a single PFGE type and phylogroup B2, corresponding to the sequence type (ST) 131 clone. Intra- and inter-species gene dissemination (CTX-M-15, CTX-M-2 and CTX-M-9) and wide epidemic spread of the CTX-M-15-producing E. coli ST131 clone in several Belgian hospitals were observed.the rapid emergence of multiresistant CTX-M-15-producing E. coli isolates is of major concern and highlights the need for further surveillance in Belgium.

Etest

Multilocus sequence typing

Enterobacter aerogenes

Molecular Epidemiology

10.1093/jac/dkq388

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Citations (71)

Evaluation of a new commercial real-time PCR assay for diagnosis of Pneumocystis jirovecii pneumonia and identification of dihydropteroate synthase (DHPS) mutations

Diagnostic Microbiology and Infectious Disease (2016)

Isabel Montesinos Marie‐Luce Delforge Farida Ajjaham Françoise Brancart Maya Hites

DHPS

Dihydropteroate synthase

Pneumocystis pneumonia

10.1016/j.diagmicrobio.2016.10.005

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Citations (43)

Evaluation of the Bio-Rad Geenius HIV-1/2 test as a confirmatory assay

Journal of Clinical Virology (2014)

Isabel Montesinos Joëlle Eykmans Marie‐Luce Delforge

Seroconversion

10.1016/j.jcv.2014.04.025

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Citations (33)

Gonococcal Aneurysm of the Ascending Aorta

Sexually Transmitted Diseases (2014)

Samuel Markowicz James Anstey Maya Hites Isabel Montesinos Sandrine Roisin

We present the case of a man with a bicuspid aortic valve who presented with persistent fever. Blood cultures yielded Neisseria gonorrhoeae, and the diagnosis of infected mycotic aneurysm was confirmed by detection of the bacterial genome in the aortic wall. The patient was cured with surgery and intravenous ceftriaxone.

Neisseria gonorrhoeae

Mycotic aneurysm

Blood Culture

10.1097/olq.0000000000000079

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Citations (2)

EchiNam: multicenter retrospective study on the experience, challenges, and pitfalls in the diagnosis and treatment of alveolar echinococcosis in Belgium

European Journal of Clinical Microbiology & Infectious Diseases (2024)

P Plum Nathalie Ausselet François Kidd Séverine Noirhomme Maria-Grazia Garrino

Abstract Objectives The aim of this retrospective study was to collect epidemiological, clinical, laboratory, imaging, management, and follow-up data on cases of alveolar echinococcosis (AE) diagnosed and/or followed up within the Namur Hospital Network (NHN) in order to gather information on the challenges, pitfalls, and overall experience in the diagnosis and treatment of AE. Methods EchiNam was a multicenter retrospective study. Patients diagnosed and/or treated for probable or confirmed AE in the NHN between 2002 and 2023 were included in the study. Patient selection was based on diagnosis codes, laboratory results, and albendazole (ABZ) dispensing. Results A total of 22 AE cases were retrieved, of which four were classified as probable and 18 as confirmed cases. Nine patients were either asymptomatic or had symptoms attributed to another disease. Clinical examination yielded pathologic findings in 10 patients. The median duration from the first AE-suggestive laboratory abnormalities to diagnosis was 176 days, and the median duration from the first AE-related imaging abnormalities to diagnosis was 133 days. Overall, 12 patients underwent surgical resection, with only four achieving complete lesion resection. Nine patients experienced ABZ-related adverse effects, with temporary ABZ discontinuation in five. Conclusion Due to various factors such as a long incubation period and a lack of awareness among Belgian physicians, AE is often diagnosed at advanced disease stages. Treatment then becomes more complex or even suboptimal, resulting in prolonged therapy, higher risk of adverse effects, significantly impaired quality of life, poor prognosis, and higher mortality rates. Measures should be taken to achieve early diagnosis in endemic areas.

Discontinuation

Albendazole

10.1007/s10096-024-04996-4

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Diagnostic Value of Serum Biomarkers for Invasive Aspergillosis in Haematologic Patients

Journal of Fungi (2024)

Isabel Montesinos Imane Saad Albichr Elodie Collinge Bénédicte Delaere Te‐Din Huang

Background: Invasive aspergillosis (IA) is a significant cause of morbidity and mortality in patients with haematological malignancies. Accurate diagnosis of IA is challenging due to non-specific symptoms and the impact of antifungal prophylaxis on biomarker sensitivity. Methods: This retrospective study evaluated the diagnostic performance of three serum biomarkers: Aspergillus Galactomannan Ag VirClia Monotest® (VirClia), Wako β-D-Glucan Test® (Wako BDG), and MycoGENIE Real-Time PCR® (MycoGENIE PCR). True positives were defined as patients with proven or probable IA (n = 14), with a positive Platelia Aspergillus Antigen® (Platelia) serving as a mycological criterion. True negatives were identified as patients with a positive Platelia assay but classified as non-probable IA (n = 10) and outpatients who consistently tested negative with the Platelia test throughout the study period (n = 20). Results: Most patients diagnosed with proven or probable IA were acute myeloid leukaemia or myelodysplastic syndrome patients receiving mould-active antifungal prophylaxis or treatment (71%). VirClia demonstrated high sensitivity (100%) for detecting IA, with a specificity of 83%. Wako BDG and MycoGENIE PCR showed lower sensitivities for IA (57% and 64%, respectively). MycoGENIE PCR detected Aspergillus spp. and Mucorales in two patients. Conclusions: Accurate diagnosis of IA remains challenging, especially in patients who have received mould-active antifungal treatment. VirClia showed comparable performance to Platelia, suggesting its potential for routine use. However, Wako BDG and MycoGENIE PCR results were less favourable in our study cohort. Nevertheless, MycoGENIE PCR detected two probable co-infections with Aspergillus spp. and Mucorales.

Galactomannan

Mucorales

10.3390/jof10090661

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Citations (0)

[Pleural empyema caused by E coli, in a child. An immunodeficient subject?].

PubMed (1978)

Pinto Lf Cristhian Gonzalo Aspiazu Briones Isabel Montesinos

Source

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Emergence of clonally related Klebsiella pneumoniae isolates of sequence type 258 producing KPC-2 carbapenemase in Belgium

Journal of Antimicrobial Chemotherapy (2009)

Pierre Bogaerts Isabel Montesinos Hector Rodríguez-Villalobos Laurent Blairon A. Deplano

Sir, The Klebsiella pneumoniae carbapenemase (KPC) is a class A b-lactamase that confers resistance to virtually all b-lactams including carbapenems. Initially reported in the USA in a Klebsiella isolate in 1996, KPC-producing K. pneumoniae have been responsible for large nosocomial outbreaks in the USA, Israel and Greece where these isolates are now endemic in hospitals. The rapid dissemination of KPC enzymes among and across different enterobacterial species is likely to be due to the localization of blaKPC genes on transferable broad host range plasmids and their association with a transposon, but is also linked with a disseminated international clone of KPC-producing K. pneumoniae isolates of sequence type (ST) 258. Here we report on the first three cases of KPC-2-producing ST258 K. pneumoniae that were detected in Belgium, subsequent to the transfer of patients from three Greek hospitals. In 2009, a patient with high-grade bladder neoplasm with pelvic metastases was transferred from a hospital in Greece to St-Pierre University Hospital in Brussels. Six weeks later, a patient with severe trauma was transferred from another hospital in the same region of Greece to the intensive care unit (ICU) of the same Brussels hospital. Finally, a few days later, the third polytrauma patient was transferred from the ICU of a hospital in Rhodes to the ICU of Erasme University Hospital in Brussels. Upon admission, a urine culture for the first patient and rectal swabs from the two other patients revealed the presence of multidrug-resistant K. pneumoniae (isolates Hk1, Hk2 and Rh1, respectively) with reduced susceptibility to carbapenems. No secondary local transmission occurred in the two hospitals following the rapid implementation of strict infection control measures. MICs produced using the Vitek2 system (N046 AST cards) and Etest (AB BIODISK, Solna, Sweden) were interpreted according to clinical breakpoints from the European Committee for Antimicrobial Susceptibility Testing (EUCAST). The three multidrug-resistant isolates showed high-level resistance to all penicillins (including temocillin) and to all expanded-spectrum cephalosporins except cefepime to which they all remained borderline susceptible (MICs of 8–16 mg/L) (Table 1). Interestingly, the three isolates appeared to be of intermediate susceptibility to meropenem using the Vitek2 system (MIC of 4–8 mg/L) while they were resistant to meropenem by Etest (MIC of 32 mg/L). All the isolates remained susceptible to gentamicin but were of intermediate

10.1093/jac/dkp453

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Citations (27)

Epidemiology and clinical impact of viral, atypical, and fungal respiratory pathogens in symptomatic immunocompromised patients: a two-center study using a multi-parameter customized respiratory Taqman® array card

European Journal of Clinical Microbiology & Infectious Diseases (2019)

Deborah Steensels Marijke Reynders Patrick Descheemaeker Martin D. Curran Maya Hites

Medical microbiology

Rhinovirus

10.1007/s10096-019-03579-y

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Citations (3)

Rhabdomyolysis and Acute Kidney Injury as Leading COVID-19 Presentation in an Adolescent

The Pediatric Infectious Disease Journal (2020)

Nathalie Tram Benedetta Chiodini Isabel Montesinos Alfredo Vicinanza Xavier Beretta‐Piccoli

Severe acute respiratory syndrome coronavirus 2, the virus responsible of the current COVID-19 pandemic, has limited impact in the pediatric population. Children are often asymptomatic or present mild flu-like symptoms. We report the case of a COVID-19-infected adolescent presenting severe rhabdomyolysis and acute kidney injury without any fever or respiratory symptoms.

Pandemic

2019-20 coronavirus outbreak

Coronavirus

10.1097/inf.0000000000002853

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Citations (20)