Yingying Mao

Zhejiang Chinese Medical University

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Co-Authors

Ding Ye

Zhejiang Chinese Medical University

Xiaohui Sun

Zhejiang Chinese Medical University

Zhixing He

Zhejiang Chinese Medical University

Yu Qian

Zhejiang Chinese Medical University

Chengping Wen

Zhejiang Chinese Medical University

Satya P. Kunapuli

Temple University

Mingjuan Jin

Second Affiliated Hospital of Zhejiang University

Ye Ding

Chinese Academy of Sciences

Ying‐Hao Han

Heilongjiang Bayi Agricultural University

Hongwei Li

Southern Medical University

Cooperative Institutions

Zhejiang Chinese Medical University

190

Zhejiang University

138

Chinese Academy of Sciences

Sun Yat-sen University

Nanjing Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sichuan University

Southern Medical University

John Wiley & Sons (United States)

Hudson Institute

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Evaluating the impact of growth differentiation factor 15 on the risk of psoriasis

International Journal of Rheumatic Diseases (2024)

Yuqing Jiang Jiayu Li Baojie Hua Yudan Yang Ying Chen

Impact factor

10.1111/1756-185x.15316

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Effects of Short-term Huatou Chan Training on Health

Fei Luo Victor Chiang Yibing Wu Yuzheng Wang Wenhong Tian

Previous studies have shown that perennial Chan training leads to improvements in brain functioning. However, few studies have investigated the effects of short-term Huatou Chan training. The current study explored the effects of a three-day Huatou Chan training on physical and emotional health, as well as brain state. Seventy healthy subjects were recruited and divided into two groups: the Huatou Chan group and the Control group. The Huatou Chan group received a 3-day Huatou Chan training, while the Control group waited for three days. Both groups completed a 6-min Brain State Index recording, the SCL-90, the Brief Profile of Mood State, the Meaning in Life Questionnaire, and the Index of Well-being, prior to and after the training or waiting period. Results showed that short-term Huatou Chan training had significant benefits on physical and emotional health (somatization, obsessive-compulsive, depression, hostility, and psychoticism), negative emotions (tension-anxiety, depression-dejection, anger-hostility, fatigue-inertia, and confusion-bewilderment), well-being, and attitude towards life. In addition, short-term Chan training can significantly improve brain state, as shown by the index of depression, anxiety, alerting, intelligence, and sleep disorder. To our knowledge, this is the first study to provide direct evidence for the benefits of short-term intensive Huatou Chan training on physical and mental health.

Hostility

Psychoticism

10.31124/advance.9870110

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Angiotensin II Induced Cerebral Microvascular Inflammation and Increased Blood‐Brain Barrier Permeability via Oxidative Stress

The FASEB Journal (2010)

Ming Zhang Yingying Mao Ronald F. Tuma Tinatin Chabrashvili

Although angiotensin II (Ang II) has been shown to cause endothelial dysfunction, its role in inflammatory responses, especially in brain, remains unclear. The goal of this study is to investigate the mechanisms through which Ang II mediates cerebral microvascular inflammation. C57BL/6 male mice were subjected to slow pressor dose of Ang II infusion (Alzet mini‐pump) ata rate of 400ng/kg/min for 14 days. Intravital microscopic observation was used to investigate the leukocyte/endothelial interactions in pial microvessels in vivo . A superoxide scavenger 4‐hydroxy‐TEMPO (Tempol) was added to drinking water (2mmol) on day 4 th following Ang II infusion. Blood‐brain barrier (BBB) permeability was evaluated by Evans Blue extravasation ex vivo . Ang II increased (p<0.01) the leukocyte adhesion on day 4 th of infusion compared to vehicle. This effect was maintained on day 14 th (p<0.05) and also was accompanied with an increase of leukocyte rolling (p<0.01). Ang II increased the BBB permeability on day 14 th (p<0.05). Tempol attenuated the increased leukocyte/endothelial interactions and protected the BBB integrity (p<0.01). In conclusion, Ang II causes cerebral microvasculature inflammation, which leads to the increased BBB permeability. This effect of Ang II is, at least in part mediated via oxidative stress since Tempol reverses the leukocyte rolling and adhesion, and prevents the increased BBB permeability.

Extravasation

Evans Blue

Vascular permeability

Intravital microscopy

Endothelial Dysfunction

10.1096/fasebj.24.1_supplement.590.1

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Citations (3)

Air pollution, residential greenspace, and the risk of incident immune thrombocytopenic purpura: a prospective cohort study of 356,482 participants

Haematologica (2024)

Peiyang Luo Feifan Wang Jiacheng Ying Ke Liu Baojie Hua

This study investigated the associations of air pollution and residential greenspace with immune thrombocytopenic purpura (ITP) risk, along with their combined effects, in a cohort of 356,482 UK Biobank participants free of ITP at baseline. Ambient PM2.5, PMcoarse, PM10, NO2, and NOx exposures were estimated by land-use regression models and residential greenspace was calculated using land use data, defined as the percentage of outdoor greenspace surrounding each participant's home location. The hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated by using Cox proportional hazard models, and non-linear relationships were assessed using restricted cubic spline (RCS) curves. A total of 500 incident ITP cases were diagnosed during a median follow-up of 13.54 years. Long-term exposure to high ambient concentrations of PM2.5 (HR = 1.15, 95% CI: 1.04-1.28, P = 0.007), NO2 (HR = 1.23, 95% CI: 1.10-1.37, P = 1.83×10-4), and NOx (HR = 1.12, 95% CI: 1.03-1.21, P = 0.011), as well as low residential greenspace (HR = 0.77, 95% CI: 0.67-0.87, P = 7.96×10-5), were associated with an increased risk of ITP. RCS curve revealed a non-linear relationship of PM10 and NOx with ITP risk (P for non-linearity: 0.003 for PM10 and 0.030 for NOx). Participants with high air pollution and low residential greenspace had the highest risk of ITP, though no evidence of mediation or interaction effects were observed. In conclusion, long-term exposure to ambient PM2.5, PM10, NO2 and NOx may increase ITP risk, whereas residential greenspace may decrease this risk.

10.3324/haematol.2024.286328

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Modulating of ocular inflammation with macrophage migration inhibitory factor is associated with notch signalling in experimental autoimmune uveitis

Clinical & Experimental Immunology (2015)

Hongxia Yang Shicheng Zheng Yingying Mao Zhen Chen Chunfu Zheng

Summary The aim of this study was to examine whether macrophage migration inhibitory factor (MIF) could exaggerate inflammatory response in a mouse model of experimental autoimmune uveitis (EAU) and to explore the underlying mechanism. Mutant serotype 8 adeno-associated virus (AAV8) (Y733F)-chicken β-actin (CBA)-MIF or AAV8 (Y733F)-CBA-enhanced green fluorescent protein (eGFP) vector was delivered subretinally into B10.RIII mice, respectively. Three weeks after vector delivery, EAU was induced with a subcutaneous injection of a mixture of interphotoreceptor retinoid binding protein (IRBP) peptide with CFA. The levels of proinflammatory cytokines were detected by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Retinal function was evaluated with electroretinography (ERG). We found that the expression of MIF and its two receptors CD74 and CD44 was increased in the EAU mouse retina. Compared to AAV8.CBA.eGFP-injected and untreated EAU mice, the level of proinflammatory cytokines, the expression of Notch1, Notch4, delta-like ligand 4 (Dll4), Notch receptor intracellular domain (NICD) and hairy enhancer of split-1 (Hes-1) increased, but the ERG a- and b-wave amplitudes decreased in AAV8.CBA.MIF-injected EAU mice. The Notch inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) reduced the expression of NICD, Hes-1 and proinflammatory cytokines. Further, a MIF antagonist ISO-1 attenuated intraocular inflammation, and inhibited the differentiation of T helper type 1 (Th1) and Th17 in EAU mice. We demonstrated that over-expression of MIF exaggerated ocular inflammation, which was associated with the activation of the Notch signalling. The expression of both MIF and its receptors are elevated in EAU mice. Over-expression of MIF exaggerates ocular inflammation, and this exaggerated inflammation is associated with the activation of the Notch signalling and Notch pathway. Our data suggest that the MIF–Notch axis may play an important role in the pathogenesis of EAU. Both the MIF signalling pathways may be promising targets for developing novel therapeutic interventions for uveitis.

10.1111/cei.12710

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Citations (21)

Single point mutation in adeno-associated viral vectors -DJ capsid leads to improvement for gene delivery in vivo

BMC Biotechnology (2016)

Yingying Mao Xuejun Wang Renhe Yan Wei Hu Andrew Li

Rational design of AAV capsids is a simple method for enhancing AAV transduction efficiency. AAV-DJ is a highly recombinogenic hybrid vector created from DNA shuffling of eight AAV serotypes, which mediates efficient gene expression both in vitro and in vivo. AAV2 and AAV8 are the closest parental vectors of AAV-DJ and it has been reported that mutations on the 137/251/503 ubiquitination or phosphorylation sites of the AAV2 or AAV8 capsid lead to dramatic enhancement of gene delivery. Here, we aimed to find out whether the same point mutations on the AAV-DJ capsid could lead to significant improvement for gene delivery both in vitro and in vivo. We constructed three single point mutants (K137R/T251A/S503A) of AAV-DJ and the transduction efficiency of these mutants and AAV-DJ were investigated using two reporter gene systems including green fluorescent protein (GFP) and dual-luciferase (Gaussia luciferase and Firefly luciferase). Data indicated that single point mutations T251A/S503A lead to significant improvement of dual-luciferase expression in vivo after tail vein (TV) injection in mice respectively, despite limited enhancement of GFP expression in 293 T, Hela and HepG2 cells in vitro. Moreover, in vivo bioluminescence image and viral genome DNA copy number in tissue analysis showed that these mutants reserved the liver tropism characteristics, consistent with AAV-DJ. Single point mutations on the 251/503 sites of AAV-DJ capsid can lead to a significant improvement for in vivo gene expression. These enhanced AAV vectors have great potential in gene therapy applications.

Transduction (biophysics)

Bioluminescence imaging

Adeno-associated virus

10.1186/s12896-015-0230-0

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Citations (104)

The association of milk products with rheumatoid arthritis: A cross-sectional study from NHANES

Joint Bone Spine (2023)

Weiwei Chen Die Jiang Ke Liu Linshuoshuo Lyu Ying Chen

Cross-sectional study

10.1016/j.jbspin.2023.105646

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Citations (2)

Alu Methylation and Risk of Cancer: A Meta-analysis

The American Journal of the Medical Sciences (2020)

Ding Ye Danjie Jiang Xinhan Zhang Yingying Mao

Alu element

Subgroup analysis

Study heterogeneity

10.1016/j.amjms.2020.03.002

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Citations (14)

RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Applied Biological Chemistry (2020)

Ying‐Hao Han Yingying Mao Nan‐Nan Yu Meihua Jin Yinghua Jin

Abstract In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII +/+ (WT) and PrxII −/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII −/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII −/− vs. PrxII +/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

KEGG

Gene knockout

10.1186/s13765-020-00515-z

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Citations (4)

Circulating phosphorus concentration and risk of prostate cancer: a Mendelian randomization study

American Journal of Clinical Nutrition (2021)

Linshuoshuo Lv Ding Ye Jie Chen Yu Qian Alan Fu

Mendelian Randomization

Pleiotropy

10.1093/ajcn/nqab342

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Citations (14)