Kun Cui

Peking University

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i-10 index

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You Wan

Peking University

Xuetao Qi

Peking University

Ming Yi

Peking University

Jiaxin Wang

Second Affiliated Hospital of Jilin University

Shuang Cui

China Pharmaceutical University

Haojie Sun

Eye & ENT Hospital of Fudan University

Jifu Tong

Peking University

Su Fu

Peking University

Fengyu Liu

First Hospital of Shijiazhuang

Shuang Cui

Dalian University of Technology

Cooperative Institutions

Peking University

National Health and Family Planning Commission

Chinese Academy of Medical Sciences & Peking Union Medical College

Hebei University of Technology

Capital Medical University

Chinese Academy of Sciences

Fudan University

China Medical University

Tsinghua University

Sun Yat-sen University

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HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in Mice

International Journal of Molecular Sciences (2023)

Yawen Zheng Shan Shao Yu Zhang Shulu Yuan Yuanwei Xing

Chronic pain is a significant health problem worldwide. Recent evidence has suggested that the ventral hippocampus is dysfunctional in humans and rodents, with decreased neuronal excitability and connectivity with other brain regions, parallel pain chronicity, and persistent nociceptive hypersensitivity. But the molecular mechanisms underlying hippocampal modulation of pain remain poorly elucidated. In this study, we used ex vivo whole-cell patch-clamp recording, immunofluorescence staining, and behavioral tests to examine whether hyperpolarization-activated cyclic nucleotide-gated channels 2 (HCN2) in the ventral hippocampal CA1 (vCA1) were involved in regulating nociceptive perception and CFA-induced inflammatory pain in mice. Reduced sag potential and firing rate of action potentials were observed in vCA1 pyramidal neurons from CFA-injected mice. Moreover, the expression of HCN2, but not HCN1, in vCA1 decreased in mice injected with CFA. HCN2 knockdown in vCA1 pyramidal neurons induced thermal hypersensitivity, whereas overexpression of HCN2 alleviated thermal hyperalgesia induced by intraplantar injection of CFA in mice. Our findings suggest that HCN2 in the vCA1 plays an active role in pain modulation and could be a promising target for the treatment of chronic pain.

HCN channel

10.3390/ijms241813823

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A nociceptive neuronal ensemble in the dorsomedial prefrontal cortex underlies pain chronicity

Cell Reports (2022)

Xuetao Qi Kun Cui Yu Zhang Linshu Wang Jifu Tong

Pain chronicity involves unpleasant experience in both somatosensory and affective aspects, accompanied with the prefrontal cortex (PFC) neuroplastic alterations. However, whether specific PFC neuronal ensembles underlie pain chronicity remains elusive. Here we identify a nociceptive neuronal ensemble in the dorsomedial prefrontal cortex (dmPFC), which shows prominent reactivity to nociceptive stimuli. We observed that this ensemble shows distinct molecular characteristics and is densely connected to pain-related regions including basolateral amygdala (BLA) and lateral parabrachial nuclei (LPB). Prolonged chemogenetic activation of this nociceptive neuronal ensemble, but not a randomly transfected subset of dmPFC neurons, induces chronic pain-like behaviors in normal mice. By contrast, silencing the nociceptive dmPFC neurons relieves both pain hypersensitivity and anxiety in mice with chronic inflammatory pain. These results suggest the presence of specific dmPFC neuronal ensembles in processing nociceptive information and regulating pain chronicity.

Basolateral amygdala

10.1016/j.celrep.2022.111833

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Citations (28)

Irisin reprograms microglia through activation of STAT6 and prevents cognitive dysfunction after surgery in mice

Brain Behavior and Immunity (2024)

Jiaxin Wang Shuaixin Gao Su Fu Yawei Li Li Su

Postoperative cognitive dysfunction

STAT6

10.1016/j.bbi.2024.12.019

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Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons

BMC Neuroscience (2019)

Kun Cui Shuai Zhang Jinyao Sun Xueying Zhang Chong Ding

Transcranial ultrasonic stimulation is a novel noninvasive tool for neuromodulation, and has high spatial resolution and deep penetration. Although it can increase excitation of neurons, its effects on neuron are poorly understood. This study was to evaluate effect of ultrasonic stimulation (US) on neurons in vitro. In this paper, the effect of US on the excitability and voltage-dependent $$ K^{ + } $$ currents of CA1 pyramidal neurons in the rat hippocampus was studied using patch clamp. Our results suggest that US increased the spontaneous firing rate and inhibited transient outward potassium current ( $$ \varvec{I}_{\varvec{A}} $$ ) and delayed rectifier potassium current ( $$ \varvec{I}_{\varvec{K}} ) $$ . Furthermore, US altered the activation of $$ \varvec{I}_{\varvec{K}} $$ channels, inactivation and recovery properties of $$ \varvec{I}_{\varvec{A}} $$ channels. After US, the $$ \varvec{I}_{\varvec{K}} $$ activation curves significantly moved to the negative voltage direction and increased its slope factor. Moreover, the data showed that US moved the inactivation curve of $$ \varvec{I}_{\varvec{A}} $$ to the negative voltage and increased the slope factor. Besides, US delayed the recovery of $$ \varvec{I}_{\varvec{A}} $$ channel. Our data indicate that US can increase excitation of neurons by inhibiting potassium currents. Different US decreased the voltage sensitivity of $$ \varvec{I}_{\varvec{K}} $$ activation differentially. Moreover, the more time is needed for US to make the $$ \varvec{I}_{\varvec{A}} $$ channels open again after inactivating. US may play a physiological role by inhibiting voltage-dependent potassium currents in neuromodulation. Our research can provide a theoretical basis for the future clinical application of ultrasound in neuromodulation.

Neuromodulation

10.1186/s12868-018-0485-1

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Research on the urgency evaluation of medium voltage project of distribution network taking account of the correction coefficient

Jinghong Zhao Bo Yang Kun Cui Zhuoyuan Gu

A new comprehensive evaluation method for the urgency of medium voltage distribution network project considering correction factors is put forward. Based on the analytic hierarchy process, the 10kV distribution network project evaluation index system is established, influence among evaluating indicators is analyzed and influence matrix of evaluating indicators is built. After that, the calculation methods of correction factors, which are developed from the influence matrix, can make quantitative analysis for the urgency of medium voltage distribution network comprehensively. Not only the evaluating indicator importance degree to the overall evaluation is considered, but also the impact of mutual influence degree between evaluating indicators on the overall evaluation is taken into account. It is possible to give a more accurate evaluation score level for evaluation samples. With analysis of three 10kV lines power lines carrying out, the effectiveness and practicality of the method is demonstrated.

Degree (music)

10.1109/pesa.2017.8277772

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Dominant activities of fear engram cells in the dorsal dentate gyrus underlie fear generalization in mice

PLoS Biology (2024)

Kun Cui Xuetao Qi Zilong Liu Weiqi Sun Peijie Jiao

Over-generalized fear is a maladaptive response to harmless stimuli or situations characteristic of posttraumatic stress disorder (PTSD) and other anxiety disorders. The dorsal dentate gyrus (dDG) contains engram cells that play a crucial role in accurate memory retrieval. However, the coordination mechanism of neuronal subpopulations within the dDG network during fear generalization is not well understood. Here, with the Tet-off system combined with immunostaining and two-photon calcium imaging, we report that dDG fear engram cells labeled in the conditioned context constitutes a significantly higher proportion of dDG neurons activated in a similar context where mice show generalized fear. The activation of these dDG fear engram cells encoding the conditioned context is both sufficient and necessary for inducing fear generalization in the similar context. Activities of mossy cells in the ventral dentate gyrus (vMCs) are significantly suppressed in mice showing fear generalization in a similar context, and activating the vMCs-dDG pathway suppresses generalized but not conditioned fear. Finally, modifying fear memory engrams in the dDG with “safety” signals effectively rescues fear generalization. These findings reveal that the competitive advantage of dDG engram cells underlies fear generalization, which can be rescued by activating the vMCs-dDG pathway or modifying fear memory engrams, and provide novel insights into the dDG network as the neuronal basis of fear generalization.

Engram

10.1371/journal.pbio.3002679

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Development of a CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain

Science Advances (2020)

Haojie Sun Su Fu Shuang Cui Xiang-Sha Yin Xiaoyan Sun

A CRISPR-based technique achieves projection- and function-specific gene editing in the rat brain.

Brain Function

10.1126/sciadv.aay6687

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Citations (34)

Establishing 3D Mandible FEA Model via Clinical CT Images

Applied Mechanics and Materials (2010)

Wen Zheng Wu Ya Dong Chen Kun Cui Xing Jun Qin Wan Shan Wang

Aiming at the filature of titanium plates and screws after defective mandible reconstruction surgery, a method named Finite Element Analysis （FEA）was conducted in this paper through FEA software Abaqus with which a 3D model of defective mandible was established. The mandible 3D model was input into the Magics and remeshed. The mandible model was output in the format of .inp file from the Magics and import to Abaqus for converting from triangular meshes to tetrahedral meshes. The tetrahedral meshes totally had 71057 tetrahedral elements and 10720 nodes. The boundary constraints of the mandible 3D model were arranged. The force was applied separately in the direction of vertical, 15° and 30° with the occluding plane (teeth force range when occluding) to analyze the force. The mandible 3D model was established and prepared to be done a biomechanical analysis.

Mandible (arthropod mouthpart)

Tetrahedron

3d model

10.4028/www.scientific.net/amm.44-47.1952

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Citations (3)

Resonance characteristic analysis of flexible DC distribution network

IET conference proceedings. (2021)

Kun Cui Lijun Qu Zhen Yu Wen Xu Zhou Rong Zeng

10.1049/icp.2020.0391

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Impaired neuronal macroautophagy in the prelimbic cortex contributes to comorbid anxiety-like behaviors in rats with chronic neuropathic pain

Autophagy (2024)

Su Fu Haojie Sun Jiaxin Wang Shuaixin Gao Zhu Liu

A large proportion of patients with chronic pain experience co-morbid anxiety. The medial prefrontal cortex (mPFC) is proposed to underlie this comorbidity, but the molecular and neuronal mechanisms are not fully understood. Here, we reported that impaired neuronal macroautophagy in the prelimbic cortical (PrL) subregion of the mPFC paralleled the occurrence of anxiety-like behaviors in rats with chronic spared nerve injury (SNI). Intriguingly, such macroautophagy impairment was mainly observed in a FOS/c-Fos+ neuronal subpopulation in the PrL. Chemogenetic inactivation of this comorbid anxiety-related neuronal ensemble relieved pain-induced anxiety-like behaviors. Rescuing macroautophagy impairment in this neuronal ensemble relieved chronic pain-associated anxiety and mechanical allodynia and restored synaptic homeostasis at the molecular level. By contrast, artificial disruption of macroautophagy induced early-onset co-morbid anxiety in neuropathic rats, but not general anxiety in normal rats. Taken together, our work identifies causal linkage between PrL neuronal macroautophagy dysfunction and comorbid anxiety in neuropathic pain and provides novel insights into the role of PrL by differentiating its contribution in pain-induced comorbid anxiety from its modulation over general anxiety-like behaviors.

Infralimbic cortex

Elevated plus maze

10.1080/15548627.2024.2330038

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Citations (4)