Atsuhiko Inoue

Hiroshima Minami High School

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Document Type

Co-Authors

Koji Sakamoto

Nagoya University

Hideaki Ohta

Nakamura Gakuen University

Hiroshi Kozuka

Hiroshima Bunkyo University

Muneichi NAKATANI

Agricultural Research Center

Yutaka Osajima

Kirinyaga University

Tetsuro Yoshiwa

Hiroshima Minami High School

Kyozo MORIMOTO

Hiroshima Minami High School

Kenya Shibata

Western Region Agricultural Research Center

Soichi Nakatani

Shingo Kato

RIKEN BioResource Research Center

Cooperative Institutions

Hiroshima Minami High School

Kyushu University

Hiroshima Bunkyo University

Hiroshima University

Nakamura Gakuen University

Kyushu Women's University

Research Institute for Bioscience and Biotechnology

Rakuten (Japan)

Ebara (Japan)

Tohoku Medical and Pharmaceutical University

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S-Methylmethionine Sulfonium in Fruits of Citrus Hybrids

Bioscience Biotechnology and Biochemistry (1996)

Koji Sakamoto Atsuhiko Inoue Muneichi NAKATANI Hiroshi Kozuka Hideaki Ohta

The S-methylmethionine sulfonium (MMS) concentrations in fruits of citrus hybrids were measured, and found to increase during ripening of the fruit. However, three of eleven hybrids of ‘Seto unshiu’ crossed with ‘Morita ponkan’ and four of 9 hybrids of ‘Murcott’ tangor crossed with ‘Seto unshiu’ had low MMS concentrations even at late harvest stage. Crossbreeding is useful in producing new citrus fruits that have juices with the desirable characteristics of their parents without formation of dimethyl sulfide which is an off-flavor.

Citrus unshiu

Sulfonium

10.1271/bbb.60.1486

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Citations (6)

Production of Single Cells from Plant Materials and Softening by an Enzyme Infusion Method Under Reduced Pressure After Freezing

Nippon Shokuhin Kagaku Kogaku Kaishi (2004)

Koji Sakamoto Masako Ishihara Kenya Shibata Atsuhiko Inoue

植物素材を凍結後,酵素液に浸漬し,減圧することにより植物組織内部に急速に酵素を含浸する方法を考案した.本法によって短時間で植物素材を軟化,単細胞化することができた.本法および攪拌法で得られた単細胞のβ-カロテンとアントシアニンの残存率および香気成分について比較した結果,本法で得られた単細胞は攪拌法に比べ,β-カロテンおよびアントシアニンの残存率は高く,香気成分の安定性も高いことが明らかとなった.また,任意の硬さを保持した食品素材の調製も可能で新しい食品素材の開発技術として有用性は極めて高い.

10.3136/nskkk.51.395

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Citations (13)

Identification of Hydrid and Nucellar Citrus Seedlings at the Seedling Stage by Leaf Oil Compounds

Nippon Nōgeikagaku Kaishi (1997)

Koji Sakamoto Atsuhiko Inoue Muneichi NAKATANI Hiroshi Kozuka Hideaki Ohta

Seventy-five compounds in citrus leaf oils at an early seedling stage were detected by a capillary gas chromatograph-mass spectrometer. Thirty-six of sixty-two seedlings were identified for the hybrid seedlings by the characteristics of the leaves and a principal component analysis(PCA) using the leaf oil compounds as variables. Eighteen of the extracted compounds contributed highly to the identification of the nucellar and hybrid seedlings by PCA. Five compounds, cis-linalool oxide, α-copaene, decanal, β-cubebene, and Germacrene D, were important for distinguishing the hybrids by a stepwise discriminant analysis.

Decanal

10.1271/nogeikagaku1924.71.1279

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Citations (2)

Compositions of Peel Oils from Citrus Hybrids, ‘Hayaka’, ‘Southern red’, and ‘Shiranui’

Nippon Nōgeikagaku Kaishi (1997)

Koji Sakamoto Atsuhiko Inoue Tetsuro Yoshiwa Kyozo MORIMOTO Muneichi NAKATANI

‘今村温州’ (Citrus unshiu Marc.)に‘中野3号ポンカン’(Citrus reticulata Blanco)を交雑した‘早香’,‘カラ’ (Citrus unshiu Marc. × Citrus nobilis Lour.)に‘中野3号ポンカン’を交配した‘サザンレッド’,さらに‘清見’に‘中野3号ポンカン’を交配した‘不知火’(‘デコポン’, ‘キョポン’などともいう)の果皮精油成分について比較検討した. (1)交雑品種の炭化水素画分と含酸素化合物画分の比率は明らかに‘中野3号ポンカン’に近く,含酸素化合物に富む精油組成を有していることが明らかとなった. (2)炭化水素画分の精油成分を比較した場合, ‘早香’は,両親に比べ, γ-テルピネンの比率が低く,両親とのパターン類似率も低かった. ‘サザンレッド’は両親よりも若干d-リモネンの比率が低く,その分γ-テルピネンが高かった. ‘サザンレッド’と両親のパターン類似率は高かった. ‘不知火’は‘清見’と同様にγ-テルピネンが少なく, ‘清見’とのパターン類似率も高かった. (3) ‘早香’におけるアルコール類の比率は‘中野3号ポンカン’‘今村温州’より高く,アルデヒド類,エステル類の比率はほぼ‘中野3号ポンカン’と‘今村温州’の中間にあった. ‘サザンレッド’は両親に比べ,エステル類が多く,アルコール類,アルデヒド類が少なかった. ‘不知火’はほぼ‘中野3号ポンカン’と‘清見’の中間にあったが,その比率は全く異なっていた. (4) ‘早香’と‘不知火’の含酸素化合物画分の精油成分組成は,花粉親の‘中野3号ポンカン’に近かったが, ‘サザンレッド’の精油成分組成は,両親とはかなり異なることがパターン類似率から明らかとなった.

10.1271/nogeikagaku1924.71.403

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Differential Recovery of Terpene Hydrocarbons and Oxygenated Compounds from Condensates Containing Essential Oil Discharged during Concentration of Citrus Juices Using a Ceramic Membrane

Food Science and Technology Research (2003)

Koji Sakamoto Kazuyoshi Fujii Atsuhiko Inoue Hiroshi Kozuka Hideaki Ohta

Essential oils of citrus fruits have many uses in consumer products so that efficient methods for their isolation are needed. Membranes are often used in the separation process but a common problem is that the flux through the membranes decreases with time. In the present study we examined whether ceramic membranes might be better at maintaining the flux at acceptable levels. To test the membranes, we attempted differential recovery of nonpolar terpene hydrocarbons and aqueous oxygenated compounds from condensates produced as a by-product during the concentration of citrus juices. When zirconia membranes (ZrO2-UF, cut-off molecular weight 50,000 and ZrO2-MF, pore size 0.08 μm) were used, little decrease in the flux was observed during filtration. The nonpolar terpene hydrocarbons were retained while more polar oxygenated flavor compounds passed through the membrane. The percentages of alcohols, esters, and aldehydes in the permeate increased markedly compared with their percentages before filtration. When the condensate from the concentration process of citrus juice sampled from a juice factory was filtered using ZrO2-UF, the hydrocarbons was concentrated, and a water-soluble essence consisting primarily of oxygenated compounds was obtained in the permeate.

Filtration (mathematics)

Terpene

Ceramic membrane

10.3136/fstr.9.11

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Citations (15)

Studies on the Clarification of Satsuma Mandarin Juice by Ultrafiltration, Part II. Effects of Some Types of Juice Extractor on Clarification of Satsuma Mandarin Juice by Ultrafiltration.

Nippon Shokuhin Kagaku Kogaku Kaishi (1995)

Koji Sakamoto Atsuhiko Inoue Hideaki Ohta

搾汁法の異なる温州ミカンストレート果汁および濃縮還元果汁について限外濾過法による清澄化を行い,それらの透過流束の変化および得られた清澄果汁の品質について検討した.(1) いずれの膜においても,遠心分離によるパルプ質除去効果か認められた.(2) 果汁別でみると,いずれの膜においてもインラインストレート果汁>インライン濃縮還元果汁>チョッパーパルパーストレート果汁の順で透過流束は高かった.(3) 得られた清澄果汁の糖,有機酸およびアミノ態窒素含量は大きな差は認められなかったが,精油成分および色調に差が認められた.すなわち,ジルコニア膜処理果汁はポリスルホン膜処理果汁に比べて,色調は淡く,テルペン系炭化水素類は極めて少なかった.また,果汁別ではインラインストレート清澄果汁は良好な香りを有していたのに対し,濃縮還元果汁およびチョッパーパルパーストレート果汁の清澄果汁の香りは,テルヘン系炭化水素類が減少することにより,温州ミカンらしい香気が低下し,反対に加熱臭,青草臭などの異臭が感じられるため品質的に劣っていた.本研究は農林水産省「特定農産物緊急技術開発事業(カンキツ)」

Ultrafiltration (renal)

Extractor

Fruit juice

Mandarin Chinese

Citrus unshiu

10.3136/nskkk.42.619

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Proportions of Peel Oil Components in Hybrid Citrus Seedlings.

Nippon Nōgeikagaku Kaishi (1994)

Koji Sakamoto Atsuhiko Inoue Tetsuro Yoshiwa Kyozo MORIMOTO Soichi Nakatani

Six hybrids of 'Seto unshiu' (Citrus unshiu Marc.) crossed with 'Morita ponkan' (Citrus reticulata Blanco) as the pollen parent were produced. Peel oil from fruit-rind tissue was prepared by simultaneous distillation and extraction under reduced pressure. Each peel oil was separated into its hydrocarbon and oxygenated-compound fractions in a silica gel column. The amount of peel oil components in the hybrids and their parents as a percentage of total peel oil was found with a capillary gas chromatograph-mass spectrometer were determined. The proportions of the hydrocarbon fractions of all hybrids were similar to the proportion in 'ponkan.' The proportions of oxygenated-compound fractions of the hybrids mostly were midway between the proportions of their parents. However, some of the hybrids had higher percentages of thymol, sinensal, and linalool than their parents.

Citrus unshiu

10.1271/nogeikagaku1924.68.815

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Citations (2)

Studies on Clarification of Satsuma Mandarin Juice by Ultrafiltration, Part I. Effect of Essential Oils on Clarification of Satsuma Mandarin Model Juice by Ultrafiltration.

Nippon Shokuhin Kagaku Kogaku Kaishi (1995)

Koji Sakamoto Atsuhiko Inoue Hideaki Ohta

高分子膜およびセラミック製無機膜を用いて温州ミカンモデル果汁の清澄化を行い,精油が透過流束に及ぼす影響および精油成分の透過特性について検討した.(1) ポリスルホン膜で処理した場合,精油は膜面へ吸着し,透過流束を低下させた.ペクチンが精油と共存すれば,精油は透過流束に影響を及ぼさないことが明らかになった.一方,ジルコニア膜処理においては,精油の影響は全く認められなかった.(2) ポリスルホン膜で処理した場合,精油の主要構成成分であるテルペン系炭化水素類の透過率は10%以下であった.一方,テルペン系アルコールであるリナロールは大部分透過した.他方,ジルコニア膜処理ではテルペン系炭化水素類はほとんど透過せず,テルペン系アルコールは透過率は比較的高かった.

Ultrafiltration (renal)

Mandarin Chinese

Fruit juice

10.3136/nskkk.42.687

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Citations (2)

Development of identification technology of Kjellmaniella crassifolia by mitochondrial DNA analysis

Shingo Kato Atsuhiko Inoue Daisuke Yasokawa

Identification

Source

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Comparison of Essential Oil Components between Leaf and Peel in Citrus Hybrids ('Seto unshiu'*'Morita ponkan').

Food Science and Technology International Tokyo (1997)

Koji Sakamoto Atsuhiko Inoue Muneichi NAKATANI Hiroshi Kozuka Hideaki Ohta

The leaf oil components in hybrid seedlings of ‘Seto unshiu’ (Citrus unshiu MARC.) crossed with ‘Morita ponkan’ (Citrus reticulata BLANCO) as the pollen parent were examined. The leaf and peel oil components in the hybrid seedlings were also compared with those of their parents. Twenty eight of the 32 peaks found in the hydrocarbon fraction of the leaf oils were identified. The major compounds were γ-terpinene and β-caryophyllene in ‘Seto unshiu’ and sabinene and β-ocimene in ‘Morita ponkan.’ All 40 peaks found in the oxygenated compound fraction of the leaf oils were identified. The major compounds were linalool, (Z)-3-hexenal, and (Z)-2-hexenal. There was a correlation between the leaf oil and peel oil in the percentage of the oxygenated compound fraction in the hybrid seedlings. A similar correlation was found for the parents. When the hybrids were classified according to the major kinds of monoterpenes, such as sabinene and γ-terpinene, and thymol and α-sinensal, the same groupings were obtained with the leaf and peel oils. These results suggested that the flavor of the fruit can be predicted by analyzing the oil in the leaf, which can be sampled much earlier than the fruit.

Citrus unshiu

Sabinene

Fraction (chemistry)

10.3136/fsti9596t9798.3.329

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Citations (2)