Mario Párraga

University of Valparaíso

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Jesús del Mazo

Centro de Investigaciones Biológicas Margarita Salas

Sebastián San Martín

University of Valparaíso

Joan Villena

University of Valparaíso

Iván Montenegro

Viña del Mar University

Luis A. López‐Fernández

Hospital General Universitario Gregorio Marañón

Alejandro Madrid

Universidad de Playa Ancha de Ciencias de la Educación

Ivo Carrasco‐Wong

San Sebastián University

Marta S. Riffo

Centro de Biología Molecular Severo Ochoa

Eva Madrid

University of Valparaíso

Manuel Valenzuela

Universidad Central de Chile

Cooperative Institutions

University of Valparaíso

University of Chile

Consejo Superior de Investigaciones Científicas

Valparaiso University

Pontificia Universidad Católica de Chile

Universidad Autónoma de Madrid

Centro de Investigaciones Biológicas Margarita Salas

Viña del Mar University

Pontificia Universidad Católica de Valparaíso

Universidade Federal Rural do Rio de Janeiro

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Effects of disinfestation, cold exposure and explant maintenance at different light conditions on in vitro tuberization of potato (Solanum tuberosum L.).

Ricardo Motta Miranda Mercedes Prado F. G. Silva A. C. Costa Mario Párraga

Solanum tuberosum

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Citations (1)

Kalanchoe pinnata (Lam.) Pers. Leaf ethanolic extract exerts selective anticancer activity through ROS-induced apoptotic cell death in human cancer cell lines

BMC Complementary Medicine and Therapies (2024)

Nicolas Faundes-Gandolfo Carlos Jara‐Gutiérrez Mario Párraga Iván Montenegro Waleska Vera

The leaves of Kalanchoe pinnata (Lam.) Pers. (K. pinnata), a succulent plant native to tropical regions, are used as a medicinal alternative against cancer in several countries worldwide; however, its therapeutic potential to fight cancer has been little addressed. In this study, we analyzed the phytochemical content, antioxidant capacity, and selectivity of K. pinnata leaf ethanolic extract against different human cancer cell lines in vitro.

Kalanchoe

Phytochemical

Crassulaceae

Cancer cell lines

10.1186/s12906-024-04570-7

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Citations (1)

Effect of normobaric hypoxia on the testis in a murine model

Andrologia (2012)

Eva Madrid Juan G. Reyes B Hernandez J. M. García Sebastián San Martín

High-altitude hypoxia generates spermiogram impairment due to germinal epithelium, Leydig cells, sperm and seminal plasma alterations, but precise mechanisms involved are unknown. The objective of this work was to analyse the effect of normobaric hypoxia on the morphology of testicular interstitium and some associated molecular and hormonal factors. Twenty-four mice were exposed to normobaric hypoxia (8.1% inspired oxygen fraction) during 20 days. The effects on body weight, testicular weight, vascularisation, testosterone, HIF1-α and VEGF were analysed at different periods of exposure and compared to controls. Hypoxic mice had lower body weight than mice kept in normoxia. Testicular weight raised significantly the 1st day, but remained normal during the rest of experiment. Number of blood vessels per field and mean diameter of vessels were higher in hypoxic mice. Plasmatic and testicular testosterone raised during first 24 h of hypoxia, but decreased on the 5th day. Vascular/interstitial ratio (proportion of interstice occupied by blood vessels) duplicated at the end of the experiment. Most substantial early effects of hypoxia were testicular oedema, increase in number and diameter of blood vessels and elevation of plasmatic and testicular testosterone. Normobaric hypoxia generates similar effects to those induced by hypobaric hypoxia.

Hypoxia

Germinal epithelium

Testicle

10.1111/and.12019

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Citations (18)

STAT3 and SOCS3 expression patterns during murine placenta development

European Journal of Histochemistry (2013)

Sebastián San Martín J.S. Fitzgerald M. Weber Mario Párraga Tamara Sáez

Signal transducers and activators of transcription 3 (Stat3) has been identified as an important signal transducer in the invasive phenotype of the trophoblasts cells in in vitro studies. However, the in situ distribution and patterns of expression of this molecule in trophoblast cells during the development of the placenta are still under-elucidated. Mice uteri of gestational ages between 7 and 14 days of pregnancy (dop) were fixed in methacarn and processed with immunoperoxidase techniques for detection of Stat3 and its phosphorylation at serine (p-ser727) residues, as well as the suppressor of cytokine signaling 3 (Socs3) expression. Stat3 was observed at 7 through 9 dop in both the antimesometrial and mesometrial deciduas, while continued immunoreactivity between 10 and 13 dop was seen only in the mesometrial decidua. In the placenta, Stat3 was detected in the cytotrophoblast cells of labyrinth and giant trophoblast cells between 10 and 14 dop. Immunoreactivity for Stat3 was also seen in trophoblast cells surrounding the maternal blood vessels. On days 10 and 11 of pregnancy, p-ser727 was detectable in the mesometrial decidua and in giant trophoblasts, while during 12-14 dop in the spongiotrophoblast region. In addition, Socs3 was immunodetected in maternal and placental tissues, principally in the giant trophoblast cells during the whole period of the study. The present in situ study shows the distribution of Stat3, its serine activation and Socs3 in different maternal and fetal compartments during murine placental development, thus further supporting the idea that they play a role during physiological placentation in mice.Â

10.4081/ejh.2013.e19

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Citations (17)

Expression of stress inducible protein 1 (Stip1) in the mouse testis

Molecular Reproduction and Development (2006)

Sefika C Mizrak Jan Bogerd Pedro P. López‐Casas Mario Párraga Jesús del Mazo

Abstract Phthalate esters are considered endocrine disruptors that interfere with the endocrine balance and development of the mammalian testis. Mono‐2‐ethylhexyl phthalate (MEHP), the active metabolite of the ubiquitously used plasticizer di‐2‐ethylhexyl phthalate (DEHP), acts upon Sertoli cells as initial target. By subtractive cDNA libraries we identified genes deregulated as response to MEHP in primary cultures of mouse Sertoli cells. The expression of mouse stress inducible protein 1 (Stip1) was detected as upregulated as a result of MEHP exposure. Stip1 is a cochaperone protein that is homologous to the human heat shock cognate protein 70 (hsc70)/heat shock protein 90 (hsp90)—organizing protein (Hop). To assess the presence and localization of Stip1 in mouse testis and its potential role in stress defense, we studied the expression pattern of the Stip1 protein by immunohistochemistry and of the mRNA by in situ hybridization. Both the protein and the mRNA of Stip1 were mainly found in the cytoplasm of all types of spermatogonia and spermatocytes up till zygotene, the expression decreased during late pachytene and was very weak in diplotene spermatocytes and round spermatids. Interestingly, this expression pattern resembled the pattern of stress sensitivity of spermatogenic cells in that the most sensitive cell types show the weakest expression of Stip1. This suggests an important role for Stip1 in the ability of germ cells to survive in stress conditions including high temperatures. Mol. Reprod. Dev. 73: 1361–1366, 2006. © 2006 Wiley‐Liss, Inc.

Dibutyl phthalate

HSP60

Suppression subtractive hybridization

10.1002/mrd.20548

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Citations (15)

Naked DNA immunization as an approach to target the generic tumor antigen survivin induces humoral and cellular immune responses in mice

Immunobiology (2005)

Álvaro Lladser Mario Párraga Licarallén Quevedo Marı́a Carmen Molina Soledad Silva

Survivin

Tumor Antigen

10.1016/j.imbio.2005.08.002

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Citations (21)

Ilf2 is regulated during meiosis and associated to transcriptionally active chromatin

Mechanisms of Development (2002)

Luis A. López‐Fernández Mario Párraga Jesús del Mazo

Bivalent (engine)

Spermatocyte

10.1016/s0925-4773(01)00612-8

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Citations (21)

Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

Journal of Experimental Zoology (1997)

Marta S. Riffo Mario Párraga

Phospholipase A2 (PLA2), its localization on human sperm and its involvement in sperm-egg interaction, was investigated. Sperm-egg interaction was examined using an in vitro assay of the interaction between human sperm and zona-free or zona-intact hamster egg. PLA2- specific antibodies and/or lysophosphatidylcholine (LPC) were added to the coincubation medium. PLA2 was localized on the anterior tip of the human sperm head by an immunogold silver staining method in light microscopy (IGSS) and TEM. PLA2-specific antibodies inhibited human sperm-zona-free oocyte fusion significantly. LPC treatment allows interspecies fertilization of zona-intact hamster oocytes. PLA2 plays an important role in membrane-fusion events. This statement is supported by the fact that PLA2 is localized in the region where an exocytotic event, such as the acrosome reaction, occurs in the spermatozoon. PLA2-specific antibodies inhibited sperm-egg fusion, but not sperm-oolemma adhesion. LPC may stimulate the fertilizing ability of spermatozoa and induce changes on the zona pellucida and on the oolemma promoting in sperm-egg fusion. Based on these findings, it is suggested that sperm PLA2 and one of its modulators, the LPC, may contribute to membrane-fusion events in mammalian fertilization.

Lysophosphatidylcholine

Acrosome reaction

Spermatozoon

Oocyte activation

Gamete

10.1002/(sici)1097-010x(19970901)279:1<81::aid-jez8>3.0.co;2-y

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Citations (41)

Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells

Evidence-based Complementary and Alternative Medicine (2018)

Manuel Valenzuela Lorena Bastias Iván Montenegro Enrique Werner Alejandro Madrid

Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT‐29 and SW‐480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT‐PCR. In the former, GJE reduced cell viability in both cell lines in a dose‐dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP‐2 and MMP‐9 gene expression diminished depending on extract and on cell type. Conclusions . These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.

Viability assay

MMP2

MMP9

Grape seed extract

10.1155/2018/2517080

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Citations (16)

P380Usselfulness of cardiac magnetic resonance imaging in limble-girdle muscular dystrophy with cardiac involvement

European Heart Journal - Cardiovascular Imaging (2019)

Rienzi Díaz‐Navarro Mario Párraga

Background/Introduction: Limb-girdle muscular dystrophy 1B (LGMD1B) is an inherited muscle disease caused by a mutation in the Lamin A/C (LMNA) gene.Cardiac involvement in patients with LGMD1B may be silent, and sudden death may occur as the first manifestation of this disease.Although electrocardiogram (ECG) and transthoracic echocardiogram (TTE) are the classic screening tools, cardiac magnetic resonance imaging (CMRI) has the advantage of being able to detect subclinical myocardial fibrosis when the results of ECG and TTE are normal.This finding may warrant the institution of cardioprotective medical treatment, because the onset of heart failure is unpredictable.Purpose: We present a case report of three members of a family who have an LMNA gene mutation.All have cardiac involvement that required CMRI to assess the condition of their heart.Clinical Case: Patient 1 (father) was evaluated in Paris in 2002, at the age of 39, together with his two sons, who had both been diagnosed with Duchenne muscular dystrophy in early childhood.He had no evidence of a muscular disorder, but genetic testing demonstrated that he had two pathogenic mutations in the LNMA gene.In July 2017, he suffered a cardiac arrest caused by ventricular fibrillation, and underwent successful cardiac resuscitation.Coronary angiography did not identify any coronary lesions.CMRI showed normal right and left ventricle (LV).Late post-gadolinium enhancement (LGE) imaging demonstrated focal epicardial enhancement in the lateral wall of the LV.A cardioverter-defibrillator was implanted.Patient 2 (propositus; older son) had from early childhood shown disorders in walking.In 2002, at the age of 15, during clinical evaluation in Paris, he was found to have proximal involvement with pseudohypertrophy.His creatine phosphokinase (CPK) concentration was 3000 U/L) (upper limit normal <398 U/L).No evidence of cardiac compromise was found.Sequencing of the LNMA gene disclosed two pathogenic mutations.In 2017, he began to experience palpitations.The ECG was unremarkable, while TTE showed LV dilatation and diminished LV function.Cine-CMRI showed a dilated LV with decreased LV function (EF 33%).LGE imaging demonstrated epicardial enhancement in the inferolateral wall of the LV.Patient 2 (younger son) was found to have proximal weakness and pseudohypertrophy of the thighs during his clinical evaluation in Paris in 2002 at the age of 11.No cardiac compromise was evident.CPK was elevated with a value of 5000 U/L.Sequencing of the LNMA gene disclosed two pathogenic mutations.In a cardiac evaluation in 2017, his ECG was normal and a TTE showed normal LV size and mildly diminished LV function.CMRI showed normal LV volumes and diminished LV function (EF 42%).LGE imaging demonstrated epicardial enhancement in the inferolateral wall of the LV.Conclusion: CMRI appears to be the most sensitive diagnostic method to assess cardiac involvement in patients with LGMD1B.

Limb-girdle muscular dystrophy

Cardiac magnetic resonance

10.1093/ehjci/jez109.021

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