The genome of a red fox (Vulpes vulpes) was recently sequenced and assembled using next-generation sequencing (NGS). The assembly is of high quality, with 94X coverage and a scaffold N50 of 11.8 Mbp, but is split into 676,878 scaffolds, some of which are likely to contain assembly errors. Fragmentation and misassembly hinder accurate gene prediction and downstream analysis such as the identification of loci under selection. Therefore, assembly of the genome into chromosome-scale fragments was an important step towards developing this genomic model. Scaffolds from the assembly were aligned to the dog reference genome and compared to the alignment of an outgroup genome (cat) against the dog to identify syntenic sequences among species. The program Reference-Assisted Chromosome Assembly (RACA) then integrated the comparative alignment with the mapping of the raw sequencing reads generated during assembly against the fox scaffolds. The 128 sequence fragments RACA assembled were compared to the fox meiotic linkage map to guide the construction of 40 chromosomal fragments. This computational approach to assembly was facilitated by prior research in comparative mammalian genomics, and the continued improvement of the red fox genome can in turn offer insight into canid and carnivore chromosome evolution. This assembly is also necessary for advancing genetic research in foxes and other canids.
The number of de novo genome sequence assemblies is increasing exponentially; however, relatively few contain one scaffold/contig per chromosome. Such assemblies are essential for studies of genotype-to-phenotype association, gross genomic evolution, and speciation. Inter-species differences can arise from chromosomal changes fixed during evolution, and we previously hypothesized that a higher fraction of elements under negative selection contributed to avian-specific phenotypes and avian genome organization stability. The objective of this study is to generate chromosome-level assemblies of three avian species (saker falcon, budgerigar, and ostrich) previously reported as karyotypically rearranged compared to most birds. We also test the hypothesis that the density of conserved non-coding elements is associated with the positions of evolutionary breakpoint regions.We used reference-assisted chromosome assembly, PCR, and lab-based molecular approaches, to generate chromosome-level assemblies of the three species. We mapped inter- and intrachromosomal changes from the avian ancestor, finding no interchromosomal rearrangements in the ostrich genome, despite it being previously described as chromosomally rearranged. We found that the average density of conserved non-coding elements in evolutionary breakpoint regions is significantly reduced. Fission evolutionary breakpoint regions have the lowest conserved non-coding element density, and intrachromomosomal evolutionary breakpoint regions have the highest.The tools used here can generate inexpensive, efficient chromosome-level assemblies, with > 80% assigned to chromosomes, which is comparable to genomes assembled using high-density physical or genetic mapping. Moreover, conserved non-coding elements are important factors in defining where rearrangements, especially interchromosomal, are fixed during evolution without deleterious effects.
The rapid increase in the number of chromosome-scale genome assemblies has renewed interest in chromosome evolution studies. The visualization of syntenic relationships between genomes is a crucial initial step in the study of chromosome rearrangements and evolution. There are few tools available that serve this purpose, and they can be difficult to learn. Moreover, these tools are limited in the number of species comparisons that can be visualized and the size of chromosome rearrangements identified. Thus, the development of novel visualization tools is in strong need.Here, we present syntenyPlotteR, an R package developed to visualize homologous synteny blocks in a pairwise or multispecies manner. This package contains three functions that allow users to generate publication-quality representations of syntenic relationships easily and quickly between genomes of interest.SyntenyPlotteR can be installed from CRAN with the documentation found in https://farre-lab.github.io/syntenyPlotteR/.
There has been a recent explosion in avian genomics. In December 2014 the Beijing Genomics Institute in collaboration with a number of labs worldwide (including Kent) released 48 new de-novo avian genome sequences in a special edition of Science. This has led to a complete re-evaluation of the phylogenetic tree of birds and presents the opportunity to study avian comparative genomics in far more detail than before. Most of these genome sequences however exist only as “scaffolds” i.e. the depth of sequence and length of read produces contiguous fragments of sub-chromosomal size. This impedes insight into overall genome structure, which is particularly challenging, as one of the most interesting biological features of birds is the peculiarity of their karyotype. This project is an on-going effort to map scaffold assemblies to avian chromosomes using a combination of bioinformatics and Fluorescent in situ Hybridization (FISH). This has traditionally been a very time-consuming and costly procedure, however a combination of bioinformatic approaches coupled with novel hardware innovation has deconstructed the FISH protocol and re-invented it as a high throughput, cheaper procedure. Initial work has helped to reconstruct Pigeon and Peregrine Falcon genomes and will ultimately provide insight into various unanswered questions pertaining to avian gross genome rearrangement. These include why the unique overall genomic structure of birds is so evolutionarily conserved, why intra and inter-chromosomal rearrangements happen (e.g. in response to the development of traits such as vocal learning) and what the karyotypes of extinct species such as dinosaurs may have looked like.
The phenomenon of a remarkable conservation of the X chromosome in eutherian mammals has been first described by Susumu Ohno in 1964. A notable exception is the cetartiodactyl X chromosome, which varies widely in morphology and G-banding pattern between species. It is hypothesized that this sex chromosome has undergone multiple rearrangements that changed the centromere position and the order of syntenic segments over the last 80 million years of Cetartiodactyla speciation. To investigate its evolution we have selected 26 evolutionarily conserved bacterial artificial chromosome (BAC) clones from the cattle CHORI-240 library evenly distributed along the cattle X chromosome. High-resolution BAC maps of the X chromosome on a representative range of cetartiodactyl species from different branches: pig (Suidae), alpaca (Camelidae), gray whale (Cetacea), hippopotamus (Hippopotamidae), Java mouse-deer (Tragulidae), pronghorn (Antilocapridae), Siberian musk deer (Moschidae), and giraffe (Giraffidae) were obtained by fluorescent in situ hybridization. To trace the X chromosome evolution during fast radiation in specious families, we performed mapping in several cervids (moose, Siberian roe deer, fallow deer, and Pere David's deer) and bovid (muskox, goat, sheep, sable antelope, and cattle) species. We have identified three major conserved synteny blocks and rearrangements in different cetartiodactyl lineages and found that the recently described phenomenon of the evolutionary new centromere emergence has taken place in the X chromosome evolution of Cetartiodactyla at least five times. We propose the structure of the putative ancestral cetartiodactyl X chromosome by reconstructing the order of syntenic segments and centromere position for key groups.
Understanding how mammalian genomes have been reshuffled through structural changes is fundamental to the dynamics of its composition, evolutionary relationships between species and, in the long run, speciation. In this work, we reveal the evolutionary genomic landscape in Rodentia, the most diverse and speciose mammalian order, by whole-genome comparisons of six rodent species and six representative outgroup mammalian species. The reconstruction of the evolutionary breakpoint regions across rodent phylogeny shows an increased rate of genome reshuffling that is approximately two orders of magnitude greater than in other mammalian species here considered. We identified novel lineage and clade-specific breakpoint regions within Rodentia and analyzed their gene content, recombination rates and their relationship with constitutive lamina genomic associated domains, DNase I hypersensitivity sites and chromatin modifications. We detected an accumulation of protein-coding genes in evolutionary breakpoint regions, especially genes implicated in reproduction and pheromone detection and mating. Moreover, we found an association of the evolutionary breakpoint regions with active chromatin state landscapes, most probably related to gene enrichment. Our results have two important implications for understanding the mechanisms that govern and constrain mammalian genome evolution. The first is that the presence of genes related to species-specific phenotypes in evolutionary breakpoint regions reinforces the adaptive value of genome reshuffling. Second, that chromatin conformation, an aspect that has been often overlooked in comparative genomic studies, might play a role in modeling the genomic distribution of evolutionary breakpoints.
The field of evolutionary and speciation genomics has been revolutionised by the ubiquity and availability of genomic data even for non-model organisms. The capability to sequence long-fragment DNA has particularly spurred trans-national initiatives to generate publicly available chromosome-resolved reference genomes across the Tree of Life. Initiatives such as the Darwin Tree of Life (The Darwin Tree of Life Project Consortium et al. 2022) or the European Genome Atlas (ERGA; Mazzoni, Ciofi, and Waterhouse 2023) enable researchers around the globe to address unresolved questions and pursue novel lines of research. The role of chromosomal rearrangements (CRs) in driving evolution has been a long-standing question in evolutionary biology (Berdan et al. 2023; Dobzhansky and Sturtevant 1938; King 1995; Robertson 1916; Wellenreuther and Bernatchez 2018; White 1978). CRs comprise an array of rearrangements that reorganise the linear sequence of the genome, ranging from local structural variants (SVs) such as inversions or duplications, to large-scale karyological changes, including chromosomal fusions and fissions (Berdan et al. 2023; Lucek et al. 2023). Current genomic data have already highlighted that CRs are much more common and diverse across taxa than previously thought (e.g., Damas et al. 2022; Weissensteiner et al. 2020). While CRs can now more easily be mapped within and across taxa, their potential role for evolution and species diversification has often remained enigmatic, and theoretical explorations exist for only a few types of CR (Berdan et al. 2023). At the dawn of broad genome availability for model and non-model organisms alike, our special issue aims to provide a genomic update on the evolutionary impact of various types of CRs. Specifically, our special issue asks what causes the evolution and establishment of CRs and whether these causes differ among taxa? Are CRs randomly distributed across the genome, and do they cause other chromosomal rearrangements? How can CRs promote diversification and how do they potentially lead to speciation? Is the evolutionary impact of CRs the same among different types of CRs? Finally, do CRs have a different evolutionary impact depending on whether autosomes or sex chromosomes are involved? Whether CRs are directly or indirectly involved in adaptation and diversification, detecting them and understanding the factors influencing their appearance in the first place are key to start shedding light on the evolutionary processes linked to CRs. Traditionally, CRs were detected either directly, with cytogenetic screenings (Krimbas and Powell 1992; Stebbins 1971; White 1973), or indirectly, by looking at the consequences of the rearrangements (e.g., Dobzhansky and Sturtevant 1938). However, the resolution of these techniques and/or the limitation of cross-species comparisons have restricted the study of the role of CRs for evolution. In this special issue, several contributions have successfully used chromosome-level assemblies and long-read sequencing to directly analyse CRs in a broad range of systems and linked these to evolutionary processes at different scales. Focusing on holocentric sedges of the genus Carex, Escudero et al. (2023) estimated an overall high rate of CRs across the genus. Using a dual approach, the authors then combined genome assemblies and linkage maps to identify CRs between species, to later refine the CRs using only Carex genome assemblies and an outgroup to define conserved and rearranged genomic regions. Interestingly, despite high rates of chromosome fission and fusion, longer than randomly expected syntenic blocks remained between species. Comparing the distribution of transposable elements (TEs) and genes with the two types of genomic regions, they further showed that conserved syntenic regions correlate with gene dense areas, while rearranged regions correlate with TEs, potentially pointing to hotspots of chromosome reshuffling. Going one step further, Cornet et al. (2023) compared the distribution of repetitive elements between species of two independently evolved holocentric clades - Carex sedges and Erebia butterflies - in relation to CRs that result in karyotype changes in these clades. First, using low-coverage short-read sequencing data they characterised the species-specific repeat landscape, highlighting that different repeat classes occur between the two clades. Focusing on four Erebia species, the authors collected repeat information for several populations and showed that changes in repeat landscapes are common between populations, and scale with the degree of genetic differentiation, suggesting that similar evolutionary processes may affect repeat landscapes and the rest of the genome. At a macroevolutionary scale, the clustering of species based on differences in the repeat landscape showed a general concordance with their gene-based phylogeny, although this was stronger in Carex. Moreover, using chromosome number as a proxy for inter-chromosome rearrangements, the authors found that certain types of repeats are more likely to be associated with karyotype changes. Overall, their results showed that chromosomal fusion and fission events are likely associated with different repetitive elements but the relative impact of specific repeats on karyotypic changes differs between independently evolved holocentric groups. By analysing chromosome-level genome assemblies of three medaka fishes representing three of their major karyotypes, that is, metacentric or acrocentric chromosomes with larger and smaller arm numbers, respectively as well as large metacentric chromosomes, Ansai et al. (2023) tested whether the karyotypic differences among the three species were caused by centric fusions or fissions, pericentric inversions, centromere repositioning, or tandem fusions. They identified putative centromeric repeats using continuous long reads polished with short reads, assessed tandem repeats using unassembled short reads, and used phylogenetic analyses to compare these centromere-associated repeats across the different species. In their study, they not only identified multiple different repeats enriched in centromeric and pericentromeric regions but also that some of these repetitive sequences were not conserved in all chromosomes, suggesting that centromeres on different chromosomes may have different repetitive landscapes. Moreover, they showed that these centromere-associated repeats are more likely caused by centromere repositioning, highlighting the role that this mechanism may have played in the karyotype evolution in medaka fishes, allowing evolutionary changes in chromosome shape without altering chromosome numbers. Using a different approach, Arias-Sardá, Quigley, and Farré (2023) aligned genome assemblies of 26 ruminant species and reconstructed five ancestral karyotypes, ranging from the ancestor of all ruminant species to the most recent ancestor of bovids. In doing so, the authors identified CRs, evolutionary breakpoint regions (EBRs) and syntenic blocks. The authors showed that CRs are not randomly distributed across the genome, with some ruminant chromosomes maintaining synteny for more than 50 million years, while others showed a high rate of rearrangement. Similarly to Escudero et al. (2023), Arias-Sardá and colleagues looked into the distribution of TEs and genes within syntenic and rearranged genomic regions, showing that TEs are depleted in syntenic regions. Going one step further, they used gene expression data of at least nine tissues in four species to define orthologous housekeeping genes. They found that synteny breaks are depleted of genes, while housekeeping genes tend to be located in conserved syntenic blocks. These results, combined with previous publications on carnivores and rodents (Álvarez-González et al. 2022; Corbo et al. 2022; Damas et al. 2022) highlight that synteny breaks co-localise with boundaries of topologically associated domains, suggest that syntenic regions might be regulatory blocks and only CRs not disrupting functional and essential genes can become fixed in evolution. But what happens when CRs do occur? Wang et al. (2023) identified hotspot regions in Neurospora fungi that are located in the telomeres where CRs, gene duplication and further relocation led to the appearance and evolution of lineage-specific genes. They found that 78% of such genes are within telomeric regions and that most of them evolved through regional CRs and gene rebirth. To investigate the functions of these lineage-specific genes, the authors assembled 68 experiments from 14 transcriptomic studies, covering most of the morphological stages and distinct culturing conditions in N. crassa. Although lineage-specific genes were involved as non-essential partners in various aspects of N. crassa biology, the knocked down gene mas-1 increased resistance to toxins, suggesting that lineage-specific genes might contribute to new phenotypes under specific conditions. All in all, this study highlights CRs as a source of novelty in adaptation and speciation. Chromosome rearrangements can also lead to the formation of novel chromosome structures, including neo-sex chromosomes, that is, the fusion between an autosome and a sex chromosome. In this special issue, using butterflies of the tribe Danaini, Mora et al. (2024) investigated the evolution of neo-sex chromosomes. These butterflies stand out for containing two prominent examples of fused neo-sex chromosomes: a recently evolved neo-W and another much older neo-Z chromosome. In this study, the authors combined Oxford Nanopore long reads, Illumina short reads and Illumina RNA-Seq libraries to assemble and annotate four different Danaini species. Moreover, they mapped the distribution and expression levels of genes on both sex chromosomes and autosomes, identifying sex-biased genes. They found a strong association between the presence of sex-biased genes and the likelihood of sex chromosome turnover, particularly affecting the W chromosome. Specifically, they identified clusters of genes with significantly biased expression towards either males or females, suggesting potential roles for sex chromosome evolution. This accumulation of sex-biased genes, likely influenced by mechanisms such as sexual antagonism, emerges as a critical factor contributing to CRs observed in sex chromosomes over evolutionary time scales. Finally, from a macro-evolutionary perspective, genome evolution and geographic isolation are two of the major drivers of biodiversity. Márquez-Corro et al. (2023) conducted an integrative study combining phylogeography, chromosomal evolution and ecological requirements in Carex, a plant species complex, distributed in the Western Euro-Mediterranean region. First, using RADseq data of 152 samples, the authors constructed a new phylogeny for the four studied Carex species. Combined with karyotypic data of all the samples, they showed that both molecular and cytogenetic evidence points to southern Iberia–north Africa as the evolutionary cradle for this Carex species group. In addition, landscape genomic analyses identified 74 loci correlated with variables related to local adaptation, such as temperature and precipitation. Interestingly, chromosome number was one of these potential variables correlated to climatic variables. Therefore, this study supports the hypothesis that karyotype variation, at least in species with holocentric chromosomes, could be selected towards different optima climatic regimes. Ranging from sedges and butterflies with holocentric chromosomes to fungi, fishes and mammals with monocentric chromosomes, a clear picture emerges, chromosome rearrangements are not randomly distributed across genomes; instead, they are paired with TEs and other repetitive elements. CRs are moreover hotspots for gene novelty related to new traits. If CRs are selectively advantageous or involved in reproductive isolation, they may eventually become fixed (Faria and Navarro 2010; Kirkpatrick and Barton 2006). However, while empirical observations suggest that CRs could be involved in the diversification of several taxonomic groups (Berdan et al. 2023; Lucek et al. 2023), their fixation is not easy from a theoretical point of view: If CRs cause meiotic defects in heterozygotes, they would result in hybrid dysfunction and/or sterility and likely be underdominant, especially when at low frequency (Navarro and Barton 2003). Consequently, new and strongly underdominant rearrangements are thus unlikely to spread to fixation. While weak underdominance would make fixation more likely, reproductive isolation would be weaker and rearrangements would then be less likely to promote speciation (Faria et al. 2019; Faria and Navarro 2010; Rieseberg 2001). The ambiguous role of underdominance for the fixation of CRs has consequently been referred to as the 'underdominance paradox' (Spirito 1998). Fixation may occur if CRs have a selective advantage, for instance through overdominance, or via inbreeding with strong genetic drift, which could counteract negative selection and facilitate the fixation of novel, yet underdominant CRs (Guerrero and Kirkpatrick 2014; Hedrick 1981; Navarro and Barton 2003). However, the processes and conditions under which CRs could become fixed are still elusive and have been addressed by two contributions to this special issue. The first contribution by Jay, Aubier, and Joron (2024) implements a two-island population genetic model to explore the impact of local adaptation and gene flow on the establishment of overdominant CRs that act as supergenes. Supergenes represent a particular type of polymorphism that group linked functional genetic elements, which then segregate as a single Mendelian locus (Thompson and Jiggins 2014) and are often associated with CRs, particularly inversions. Modelling inversions evolving under disruptive selection between populations resulted in an increased frequency of poorly adapted immigrant inversion haplotypes. In this model, supergenes could evolve and be maintained through balancing selection because of inversion overdominance, where inversions promote the maintenance of alternative haplotypes even when they bear unequal benefits for local adaptation. This is the case when the recombination load because of gene flow balances the inversion fitness load, such that the spread of an inversion could result in the loss of an existing locally adapted haplotype, which in turn would promote the maintenance of differentially adapted and non-recombining haplotypes. Overall, this model extends the idea that supergenes can be maintained through alternative environmental fitness optima (Schwander, Libbrecht, and Keller 2014), as the study highlights that supergenes can evolve because of the intrinsic features of inversions themselves. Using a forward-in-time simulation framework, Banse et al. (2023) explore the evolutionary outcome of populations in the presence of substitutions, insertion-deletions (InDels) and CRs. Their models highlight that CRs promote gene duplications but also reduce epistasis, which together support long-term adaptation. Here, CRs that promote gene duplications have a much higher fitness benefit in simulations that started far off the optimum, highlighting that they could support adaptive evolution. Within the simulated framework, small InDels and CRs impact genome size evolution and the adaptive potential of a species differently. While CRs allow for gene duplications, InDels do not facilitate a de novo evolution of genes but instead promote the evolution of existing genes. Consequently, if only InDels were allowed to evolve, they would introduce random genetic material, leading to larger genomes with fewer genes than in the presence of CRs. Together, the theoretical explorations of Jay, Aubier, and Joron (2024) and Banse et al. (2023) highlight that CRs can promote adaptive evolution, and therefore speciation, under various conditions, but also stress the need to develop more theoretical frameworks that go beyond inversion type CRs. While CRs are widespread across the Tree of Life, it often remains unclear whether they have been established by chance, for example, through drift or by selection. Using chromosome level genome assemblies, Mackintosh et al. (2023) explore this question in Brenthis butterflies. Based on the inferred demographic history of their focal species, they show that most CRs are likely selectively neutral or very weakly underdominant in their system. They suggest that drift is not strong enough to fix considerably underdominant rearrangements and that there is only weak evidence that chromosome fusions fixed through positive natural selection or meiotic drive. Indeed, only one chromosomal fusion event showed evidence for a strong recent selective sweep, though positive selection in the more distant past could not be excluded. Overall, Mackintosh et al. (2023) provide a novel population genetic framework that combines genome assemblies and whole-genome sequence data to study the potential contribution of drift and selection to the fixation of CRs at macroevolutionary scale. Chromosomal rearrangements can contribute to the diversification of species, for example, through the suppression of recombination (Noor et al. 2001; Rieseberg 2001) or by inducing heterozygote disadvantage (White 1973), though the two mechanisms may also operate in tandem (e.g., Yoshida et al. 2023). Inversions (Kirkpatrick and Barton 2006) and chromosomal fusions (Guerrero and Kirkpatrick 2014), are expected to locally reduce recombination, thereby potentially capturing and linking together locally adaptive alleles, which can promote species divergence even in the face of gene flow. The role of inversions in diversification is comparatively well studied (Faria et al. 2019; Wellenreuther and Bernatchez 2018), while fewer studies have focused on the impact of chromosomal fusions and fissions (but see Augustijnen et al. 2024; Escudero et al. 2023; Mackintosh et al. 2023; Mora et al. 2024), translocations (see Guerrero and Kirkpatrick (2014)), or other CRs (see Wang et al. 2023). Several contributions in this special issue expand on the roles of CRs in local adaptation, species diversification, and on the maintenance of inversion polymorphisms. For instance, Ravagni et al. (2023), characterise the presence and absence of an inversion that acts as a supergene in the common quail Coturnix coturnix on the Azores. This inversion has previously been identified in quails from Europe and Africa, including the other Macaronesian archipelagos, and is associated with the loss of migratory behaviour (Sanchez-Donoso et al. 2022). For the Azores, Ravagni and colleagues find genetic divergence among populations of quails containing the inverted and standard configuration to be minimal, although the two arrangements have coexisted for a long time in relatively large populations. The authors therefore suggest that balancing selection, rather than divergent selection or drift, maintains the frequencies of inversion polymorphism on the Azores. Indeed, migration is absent in Azorean quails whether the inversion was present or not, highlighting that the role of CRs can vary across geographical scales. The degree to which inversions, particularly those underlying repeated ecotype formation, are polymorphic across a species' range is further explored by Reeve et al. (2023). In the rough periwinkle Littorina saxatilis, as well as in the closely related Littorina arcana, inversion polymorphisms are common, and can often be tied to parallel evolution of the same ecotypes across vast geographical scales. Inversions related to the same ecotypes may nonetheless show location-dependent patterns, indicating that they may contain different sets of adaptive alleles across populations, or be influenced by different selective pressures. Altogether, this suggests a broad but complex role for inversion polymorphisms in the formation of ecotypes, given that the underlying genetic basis may not always be the same. Reeve and colleagues further develop a novel, complementary approach to detect inversions using spl in heterozygosity, which may be particularly useful in cases when reference genomes are not fully resolved, and when CRs are relatively rare. Mediterranean ecotypes of the long-snouted seahorses Hippocampus guttulatus studied by Meyer et al. (2024), are similarly linked to two large, ancient polymorphic inversions: the first inversion is alternatively fixed between marine and lagoon environments, likely through divergent selection, and may play a role in speciation in this system. The second inversion is only polymorphic in lagoon populations of the Mediterranean Sea and may be governed by a combination of pseudo-overdominance and local adaptation. However, a possible breakdown of the link between the first inversion with the marine-lagoon environment for some populations, particularly those where the second inversion is not polymorphic, could point to the presence of epistatic interactions between the two inversions. Meyer and colleagues therefore bring up interesting questions concerning the interplay of multiple CRs within segregating populations. While many CRs that have been linked to adaptive divergence seem to involve large sections of a genome (Meyer et al. 2024; Ravagni et al. 2023; Schaal, Haller, and Lotterhos 2022; Wellenreuther and Bernatchez 2018), this does not need to be always the case. Using extensive, low-coverage whole-genome data for the sockeye salmon Oncorhynchus nerka, Euclide et al. (2023) detect many small islands of divergence between ecotypes related to spawning ground. Most of these islands show divergence on a local scale and are not consistently associated with the same ecotype divergence everywhere. Only four islands of divergence are conserved across all studied populations, and three of them are likely linked to inversions, while the other seems to be a result of divergence hitchhiking. Euclide and colleagues therefore suggest that the radiation of sockeye salmon into a multitude of locally adapted populations may result from a mosaic of unique allele combinations across small genomic regions of divergence. They further show that islands of divergence do not require underlying structural variation, and that small genomic regions can be of adaptive importance. They suggest that genetic architecture and the strength of selection pressures may further influence the size of adaptive islands in the genome. Sex chromosomes can play a disproportionately large role in speciation compared to autosomes (Payseur, Presgraves, and Filatov 2018) and their evolution is often linked to CRs (Mora et al. 2024; Wright et al. 2016). Viitaniemi et al. (2023) study the effects of inversions on the Z-chromosome of the zebra finch Taeniopygia guttata on sperm morphology. As inversions can disrupt gene regulation in a tissue-specific manner (e.g., Kraft et al. 2019), they analysed gene expression in testes and the liver across several time points in testes development. The presence of the inversions on the Z-chromosome impacts expression in both testes and the liver, with heterokaryotypes showing intermediate expression or resembling one or the other homokaryotype in a gene-specific and tissue-specific manner. Taken together, the results of Viitaniemi and colleagues suggest that the regulatory effects of inversions can be pleiotropic and vary between tissues. Technical limitations have for a long time restricted the study of CRs to few model organisms and few types of CR. Recent developments, especially long-fragment sequencing have overcome this obstacle and international sequence initiatives are democratising the access to genomic resources. This special issue brings together examples of various types of CRs from across broad taxonomic diversity, studied both at a micro- and macroevolutionary level, as well as empirical and theoretical explorations, highlighting the distinct and complex nature of CRs across the Tree of Life. Going beyond classic detection methods that are based on short-read sequence data, several studies of this special issue use current state-of-the-art comparative genomic approaches to detect various CRs at genome scale. Contrasting classic predictions from gene homology maps (Nadeau and Sankoff 1998), several studies suggest that CRs are not randomly distributed across the genome, as, for example, specific synteny blocks may be less likely to undergo rearrangements (Arias-Sardá, Quigley, and Farré (2023); Escudero et al. (2023)). However, the underlying genomic and evolutionary mechanisms as well as the generality of these observations require further investigation. Indeed, both neutral processes such as drift or demographic history (Cornet et al. 2023; Mackintosh et al. 2023) as well as selection (Wang et al. 2023) can shape the diversity and distribution of CRs across genomes. CRs can nevertheless have a profound impact on the genome structure, including centromere repositioning (Ansai et al. 2023) or the evolution of sex chromosomes (Mora et al. 2024; Viitaniemi et al. 2023; Wright et al. 2016). Despite the ease at which genome-wide CRs can now be quantified, the evolutionary processes that promote their spread and potential fixation, as well as their role for speciation, have often remained enigmatic. Overcoming the classic dominance paradox, Jay, Aubier, and Joron (2024) show that inversions can be maintained through balancing selection if they are linked to supergenes. However, the impact of such supergenes may be context dependent (Ravagni et al. 2023; Sanchez-Donoso et al. 2022). Other types of CRs such as InDels may similarly establish if they are adaptive, for example, by promoting gene duplications Banse et al. (2023). Macroevolutionary investigations have the potential to identify additional extrinsic agents of selection that could have promoted the fixation of CRs among species (Lucek et al. 2023), such as temperature or precipitation Márquez-Corro et al. (2023) or demographic history (Potter et al. 2017). If and how CRs could promote local adaptation and speciation seems to be context dependent and differ among taxa, where intraspecific variation in CRs can promote repeated ecotype formation (Euclide et al. 2023; Reeve et al. 2023; Wellenreuther and Bernatchez 2018). Given that having reference genomes for most eukaryotic species is likely becoming a reality over the next decade, large-scale macroevolutionary inferences will become possible, allowing to map CRs across taxonomic genera and families and to test for their potential impact on the evolution and maintenance of biodiversity. Combining intra- and interspecific pan-genome approaches could hold the key to bridge between the micro- and macroevolutionary roles of CRs, shedding light on which CRs could promote speciation. Identifying putative target CRs further allows for experimental approaches such as CRISPR/CAS9 (Cheng et al. 2024; Yoshida et al. 2023) or nanosurgery (Blázquez-Castro, Fernández-Piqueras, and Santos 2020) to go beyond correlational associations. Understanding the evolutionary impact of CRs also requires additional theoretical explorations, which have so far especially focused on inversions (Berdan et al. 2023; Faria et al. 2019) or changes in chromosome numbers (Faria and Navarro 2010; White 1978). Here, Banse et al. (2023) provide a framework that could test the impact of other CR types such as InDels. While the impact of CRs has been primarily studied along the linear genome, the latter is also folded in a complex three-dimensional structure which can be altered by CRs. Recent technical advances now allow to study this three-dimensional structure even for non-model organisms, allowing to assess the interplay between CRs and chromosomal structure, also in the context of speciation (Mohan et al. 2024). All authors contributed to the writing of this manuscript. We would like to thank all the authors who contributed articles to this Special Issue, as well as the reviewers who evaluated the manuscripts. We further thank our executive editor Ben Sibbett for his help throughout. K.L. is supported by the Swiss National Science Foundation (SNSF) Eccellenza Project 'The evolution of strong reproductive barriers towards the completion of speciation' (Grant ID 202869) and H.A. by the SNSF grant 184934 'Genomic rearrangements and the origin of species' that was awarded to K.L. H.A. was further supported by the Burckhardt-Bürgin foundation and SNSF grant 219283 awarded to Thomas Flatt. C.A.-S. was funded by the GTA fellowship programme from the University of Kent. M.F. was funded by the Royal Society grant number RGS/R1/211047. The authors declare no conflicts of interest. This is an editorial without any associated data.
Nature Communications 5: Article number: 3966 (2014); Published online: 3 June 2014; Updated: 12 Aug 2015. In Fig. 2 of this Article, the CLOCK protein sequence of the blind mole rat (Spalax) was inadvertently used to represent that of the naked mole rat (Heterocephalus glaber) in both the sequence alignment (Fig.
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