Xingmin Aaron Zhang

University of Wisconsin–Madison

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Thomas F.J. Martin

University of Wisconsin–Madison

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University of Wisconsin–Madison

Dartmouth College

University of Kansas

The University of Texas Southwestern Medical Center

Howard Hughes Medical Institute

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Atmospheric and Environmental Research

Universidad Nacional Autónoma de México

Saarland University

University of California, San Francisco

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High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells

BIO-PROTOCOL (2017)

Xingmin Aaron Zhang Thomas F.J. Martin

Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay using DCV cargo, NPY-Venus, and an orthogonal

10.21769/bioprotoc.2680

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