Olivia Shaw

Guy's and St Thomas' NHS Foundation Trust

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Co-Authors

Nizam Mamode

NHS Blood and Transplant

Anthony Dorling

King's College London

R. S. Vaughan

Duke-NUS Medical School

Nicos Kessaris

Guy's and St Thomas' NHS Foundation Trust

Miriam Manook

Duke Medical Center

Jodi A. Hadden‐Perilla

University of Delaware

Ranmith Perera

Guy's and St Thomas' NHS Foundation Trust

David Briggs

University of Utah

Nicholas Barnett

Guy's and St Thomas' NHS Foundation Trust

Stephen D. Marks

Great Ormond Street Hospital for Children NHS Foundation Trust

Cooperative Institutions

King's College London

Guy's Hospital

University College London

Guy's and St Thomas' NHS Foundation Trust

NHS Blood and Transplant

National Health Service

St Thomas' Hospital

King's College Hospital

Great Ormond Street Hospital for Children NHS Foundation Trust

National Institute for Health Research

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Effects of selenomethionine substitutions on the GB1 model protein characterized by all-atom molecular dynamics simulations

Olivia Shaw Jodi A. Hadden‐Perilla

Dynamics

10.1021/scimeetings.0c06010

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The Effectiveness of the Enhanced Recovery Programme for Category 4 Elective Caesarean Sections within Brighton and Sussex University Hospitals

Pediatric Oncall (2017)

Olivia Shaw

10.7199/ped.oncall.2017.s4

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A new approach to HLA typing designed for solid organ transplantation: epityping and its application to the HLA‐A locus

International Journal of Immunogenetics (2013)

Emma Lougee Sejal Morjaria Olivia Shaw R. W. Collins R. S. Vaughan

Summary HLA ‐specific antibodies bind discrete clusters of amino acids called epitopes, but serological assignment of antibody specificities makes no reference to this. As HLA typing for solid organ transplantation is provided at only medium (serologically equivalent) resolution, this means that recipient HLA antibodies to donor HLA epitopes may not be identified. We have designed a novel and rapid HLA ‐A epitope typing method (epityping) using a two‐stage PCR ‐ SSP ‐based method to detect the HLA ‐A locus epitopes described by El Awar et al . 2007, Transplantation , 84 , 532. The initial PCR step utilizes HLA ‐A locus‐specific primers; the product is cleaned using the QIA quick Spin Purification procedure. The purified product is tested using our in‐house epitope‐specific primer panel, the results being visualized using gel electrophoresis. Twenty two UCLA DNA Exchange samples were epityped, blinded to the HLA type. Of the 75 primer pairs, the mean correlation coefficient was 0.95 with each sample giving 67 or more correct primer results. In all cases, it was possible to derive the first field classic HLA type from the epityping results. These results indicate that a method for identification of HLA epitopes which is comparable in time, cost and technical expertise to current HLA typing methods is achievable. Redesigning HLA typing to correlate with what the antibody binds should minimize inappropriate organ allocation. We suggest that epityping provides a more effective method than standard HLA typing for solid organ transplantation.

Histocompatibility Testing

Primer (cosmetics)

HLA-A

10.1111/iji.12053

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Citations (2)

The risk associated with T cell memory in HLA specific antibody incompatible renal transplantation

Tissue Antigens (2011)

Olivia Shaw Nicholas Barnett Shivakumar Kenchayikoppad Nizam Mamode R. S. Vaughan

Histocompatibility Testing

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Clinical risk stratification of paediatric renal transplant recipients using C1q and C3d fixing of de novo donor-specific antibodies

Pediatric Nephrology (2017)

Jon Jin Kim Olivia Shaw Chloe Martin George Michaelides Ramnath Balasubramaniam

We have previously shown that children who developed de novo donor-specific human leukocyte antigen (HLA) antibodies (DSA) had greater decline in allograft function. We hypothesised that patients with complement-activating DSA would have poorer renal allograft outcomes. A total of 75 children developed DSA in the original study. The first positive DSA sample was subsequently tested for C1q and C3d fixing. The primary event was defined as 50% reduction from baseline estimated glomerular filtration rate and was analysed using the Kaplan–Meier estimator. Of 65 patients tested, 32 (49%) and 23 (35%) tested positive for C1q and C3d fixing, respectively. Of the 32 C1q-positive (c1q+) patients, 13 (41%) did not show concomitant C3d fixing. The mean fluorescence intensity values of the original immunoglobulin G DSA correlated poorly with complement-fixing positivity (C1q: adjusted R 2 0.072; C3d: adjusted R 2 0.11; p < 0.05). C1q+ antibodies were associated with acute tubulitis [0.75 ± 0.18 (C1q+) vs. 0.25 ± 0.08 (C1q−) episodes per patient (mean ± standard error of the mean; p < 0.05] but not with worse long-term renal allograft dysfunction (median time to primary event 5.9 (C1q+) vs. 6.4 (C1q−) years; hazard ratio (HR) 0.74; 95% confidence ratio (CI) 0.30–1.81; p = 0.58]. C3d-positive (C3d+) antibodies were associated with positive C4d histological staining [47% (C3d+) vs. 20% (C3d−); p = 0.04] and with significantly worse long-term allograft dysfunction [median time to primary event: 5.6 (C3d+) vs. 6.5 (C3d−) years; HR 0.38; 95% CI 0.15–0.97; p = 0.04]. Assessment of C3d fixing as part of prospective HLA monitoring can potentially aid stratification of patients at the highest risk of long-term renal allograft dysfunction.

Nephrology

Concomitant

10.1007/s00467-017-3772-7

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Citations (19)

Epityping - a new approach to the HLA system designed for solid organ transplantation

International Journal of Immunogenetics (2010)

Emma Lougee Sejal Morjaria Olivia Shaw R. W. Collins Robert W. Vaughan

Solid organ

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Citations (1)

Adaptation of the standard Luminex single antigen bead assay to detect HLA-specific antibody to IgM and IgA

International Journal of Immunogenetics (2009)

C. O. S. Savage Sejal Morjaria Olivia Shaw Richard F. Collins Robert Vaughan

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BSHI/BTS guidance on crossmatching before deceased donor kidney transplantation

International Journal of Immunogenetics (2021)

Sarah Peacock David Briggs Martin Barnardo Richard Battle Paul Brookes

Abstract All UK H&I laboratories and transplant units operate under a single national kidney offering policy, but there have been variations in approach regarding when to undertake the pre‐transplant crossmatch test. In order to minimize cold ischaemia times for deceased donor kidney transplantation we sought to find ways to be able to report a crossmatch result as early as possible in the donation process. A panel of experts in transplant surgery, nephrology, specialist nursing in organ donation and H&I (all relevant UK laboratories represented) assessed evidence and opinion concerning five factors that relate to the effectiveness of the crossmatch process, as follows: when the result should be ready for reporting; what level of donor HLA typing is needed; crossmatch sample type and availability; fairness and equity; risks and patient safety. Guidelines aimed at improving practice based on these issues are presented, and we expect that following these will allow H&I laboratories to contribute to reducing CIT in deceased donor kidney transplantation.

10.1111/iji.12558

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Citations (6)

HLA-Incompatible Transplantation - Correlating Clinical Phenotypes & Transfusion With Histology & DSA Evolution to Predict Outcomes After Positive Cross-Match Transplantation.

Transplantation (2014)

Miriam Manook Lionel Couzi Ranmith Perera Olivia Shaw Anthony Dorling

Histocompatibility Testing

Histology

10.1097/00007890-201407151-01710

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The Clinical Spectrum of De Novo Donor-Specific Antibodies in Pediatric Renal Transplant Recipients

American Journal of Transplantation (2014)

Jae Joon Kim B. Rajkapoor George Michaelides Per Wittenhagen Neil J. Sebire

The development of donor-specific HLA antibodies (DSA) is associated with worse renal allograft survival in adult patients. This study assessed the natural history of de novo DSA, and its impact on renal function in pediatric renal transplant recipients (RTR). HLA antibodies were measured prospectively using single-antigen-bead assays at 1, 3, 6 and 12 months posttransplant followed by 12-monthly intervals and during episodes of allograft dysfunction. Of 215 patients with HLA antibody monitoring, 75 (35%) developed DSA at median of 0.25 years posttransplant with a high prevalence of Class II (70%) and HLA-DQ (45%) DSA. DSA resolved in 35 (47%) patients and was associated with earlier detection (median, inter-quartile range 0.14, 0.09-0.33 vs. 0.84, 0.15-2.37 years) and lower mean fluorescence intensity (MFI) (2658, 1573-3819 vs. 7820, 5166-11 990). Overall, DSA positive patients had more rapid GFR decline with a 50% reduction in GFR at mean 5.3 (CI: 4.7-5.8) years versus 6.1 (5.7-6.4) years in DSA negative patients (p = 0.02). GFR decreased by a magnitude of 1 mL/min/1.73 m(2) per log10 increase in Class II DSA MFI (p < 0.01). Using Cox regression, independent factors predicting poorer renal allograft outcome were older age at transplant (hazard ratio 1.1, CI: 1.0-1.2 per year), tubulitis (1.5, 1.3-1.8) and microvasculature injury (2.9, 1.4-5.7). In conclusion, pediatric RTR with de novo DSA and microvasculature injury were at risk of allograft failure.

Quartile

10.1111/ajt.12859

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Citations (78)