Olha Kravchenko

Taras Shevchenko National University of Kyiv

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Л. І. Остапченко

Taras Shevchenko National University of Kyiv

Nataliia Raksha

Taras Shevchenko National University of Kyiv

ОS

О. Savchuk

Taras Shevchenko National University of Kyiv

T. Tsarenko

Taras Shevchenko National University of Kyiv

Tatyana Synelnyk

Taras Shevchenko National University of Kyiv

O.S. Kostiuk

Tetiana Vovk

Taras Shevchenko National University of Kyiv

Tetiana Halenova

Taras Shevchenko National University of Kyiv

СА

С. А. Суходоля

National Pirogov Memorial Medical University, Vinnytsya

M. Tymoshenko

Taras Shevchenko National University of Kyiv

Cooperative Institutions

Taras Shevchenko National University of Kyiv

Bogomolets National Medical University

National Pirogov Memorial Medical University, Vinnytsya

Odessa National Medical University

Republican Research Center for Radiation Medicine and Human Ecology

Gomel State Medical University

Infectious Clinical Hospital No. 1

Danylo Halytsky Lviv National Medical University

Shupyk National Healthcare University of Ukraine

National Academy of Agrarian Sciences of Ukraine

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ЕPIDEMIOLOGICAL AND СLINICAL FEATURES OF INFLUENZA IN CHILDREN IN THE STAVROPOL TERRITORY IN THE EPIDEMIC PERIOD OF 2015–2016 AND 2016–2017 YEARS

Journal Infectology (2019)

С. М. Безроднова N. A. Yatsenkо Olha Kravchenko Sh. M. Khurtsilava

Objective: to study the clinical and epidemiological features of influenza in children in the Stavropol Territory. Materials and methods: influenza prevalence is analyzed from 2015 to 2017 the paper used the data from the Territorial Rospotrebnadzor in the Stavropol Territory. We used the following methods: bibliographic, monographic description, epidemiological, analytical, statistical methods. Results: Among the deciphered acute respiratory viral infections, the specific gravity of influenza A (H1N1) 09 in 2016 reached 78%, and in 2017 influenza B prevailed – in 57,4% of children, and influenza A (H3N2) – in 42,6% of cases. In 2016 68,5% of children under 6 years of age, of all admitted, were hospitalized, and in 2017 – 83,86%. We presented the peculiarities of the course of influenza in different epidemic seasons. Conclusion: Unvaccinated children up to 6 years of age have the disease mainly in severe forms and with complications. The epidemic period began to increase in 2015–2016 at week 52, and in 2016–2017 from week 48, ended at week 13 and at week 17. At the epidemic of 2015–2016, intoxication syndrome with chills, microcirculation disturbance, catarrhal syndrome, ARDS prevailed in the clinical picture. The epidemic season of 2016–2017 was characterized by intoxication syndrome, encephalic reaction, hemorrhagic and myalgic syndromes.

Chills

10.22625/2072-6732-2019-11-1-71-75

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Effects of IgG from the serum of ischemic stroke patients on hemostasis

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale (2021)

T. Katrii Nataliia Raksha Tetiana Halenova Tetiana Vovk Olha Kravchenko

Ischemic stroke is among the top diseases leading to mortality and disability in the world. The detailed investigation of the mechanisms underlying this pathology and especially mediating the tendency to relapse during the first year after stroke incident undoubtedly belongs to important tasks of modern medicine and biology. The current study aims to analyze the influence of IgG derived from the blood serum of ischemic stroke patients on some hemostasis factors. In total, 123 participants with IS, 62 with atherothrombotic ischemic stroke, 61 with cardioembolic ischemic stroke, and 57 subjects as control have been examined. The same patients have participated in the research a year after stroke. IgG from serum was isolated by affinity chromatography on protein A Sepharose column. The activity of key hemostasis factors under the influence of IgG was analyzed. Obtained results revealed that IgG of stroke patients but not healthy subjects caused the inhibition of the amidolytic activity of endogenously generated thrombin, protein C, factor Xa, and led to an increase in the degree of ADP-induced platelet aggregation. The reduction of clotting time in the test "Thrombin time" by IgG of patients at the acute phase of disease was also observed; IgG of healthy subjects mediated the opposite effect. In contrast to acute ischemic stroke IgG, IgG of patients one year after both atherothrombotic and cardioembolic ischemic stroke influenced only the activity of endogenously generated thrombin and factor Xa resulting in inhibition of their activities. It was also established that IgG of ischemic stroke patients, as well as healthy subjects, stimulated the secretion of tissue plasminogen activator by endotheliocytes.

Stroke

10.4081/jbr.2021.9582

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Impact of Hyperhomocysteinemia on Cytokine and Matrix Metalloproteinases Contents in Rat Skeletal Muscle

Biomedical and Biotechnology Research Journal (BBRJ) (2024)

Olha Kravchenko Nataliia Raksha Kostiuk Oleksandra Liashevska Oleksandra Tiron Oksana

Abstract Background: High levels of homocysteine (Hcy) in the blood (hyperhomocysteinemia [HHcy]) are associated with many pathologies, including atherosclerosis, coronary heart disease, stroke, neurological disorders, and a decrease in muscle mass and strength. The present study aimed to evaluate the levels of pro- and anti-inflammatory cytokines and matrix metalloproteinases (MMPs) in skeletal muscle of rats with experimental HHcy. Methods: HHcy in rats of two age groups was induced by intragastric administration of D, L-thiolactone Hcy for 8 weeks. The criterion for the development of pathological conditions was a blood Hcy level above 15 mmol L −1 . Cytokine and MMP levels in muscle homogenate were determined by enzyme-linked immunosorbent assay. Results: The findings of the study on the content of pro-inflammatory cytokines in skeletal muscles of rats with hyperhomocysteinemia indicated the following. For immature rats, the greatest reduction in tumor necrosis factor-α and interleukin-8 (IL-8) content was observed, whereas, for mature individuals, the most pronounced decline in IL-1b was evident in skeletal muscle tissue under the pathology state. The content of anti-inflammatory interferon-γ was also reduced in the muscles of both age groups by 30% compared to the control, besides a decrease in the content of collagenase MMP-1 was also observed. Conclusions: The presence of HHcy is associated with metabolic changes in skeletal muscles, which can result in a depletion of cytokines and a slowing down of proteolytic remodeling. This, in turn, can lead to the development of muscular abnormalities, including dysfunction, weakness, and muscle degeneration.

Hyperhomocysteinemia

10.4103/bbrj.bbrj_234_24

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[Phospholipid content of plasma membranes and phospholipase C activity in epithelial cells of the large intestine in colitis-associated carcinogenesis in rats].

Deleted Journal (2009)

Drobins'ka Ov Olha Kravchenko Koval'ova Va Artemenko OIu Л. І. Остапченко

The decrease of major cytoplasmic membrane phospholipids (phosphatidylcholine and phosphatidylethanolamine) content was established in mucosal epithelial cell under colon inflammation pathology--ulcerative colitis. It was shown that aforementioned changes were associated with the increase of phospholipids' hydrolyzing enzyme--phospholipase C activity and intracellular Ca2+ concentration enlargement. Carcinogenesis stimulation under inflammation was accompanied by phospholipase C activity increase when quantity of investigated phospholipids (phosphatidylcholine, phosphatidylinozytol, phosphatidylserine) separately decreased and cytoplasmic Ca2+ value normalization was established.

Phosphatidylethanolamine

Phospholipase A1

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The ornithine decarboxylase, NO-synthase activitiesand phospho-c-Jun content under experimental gastric mucosa malignancy

Biomedical Research and Therapy (2016)

M. Tymoshenko Olha Kravchenko Olesya Sokur Liudmila Gaida Yulia Omelchenkoand Liudmila Ostapchenko

Ornithine decarboxylase is the first and key regulatory enzyme in synthesis of polyamines, which are essential for cell proliferation and differentiation, so its aberrant regulation is reported to play a role in neoplastic transformation and tumours growth. That’s why, there were analysed some major links of metabolic pathways that are closely related to tumorigenesis: ornithine decarboxylase, and the NADPH-dependent enzyme nitric oxide synthase, the nuclear phosphoprotein c-Jun, that could play an important role in the development of gastric cancer malignancy. Thegastric carcinogenesis was initiated in rats by 10-week replacement of drinking water by 0.01% N-methyl-N’-nitro-N-nitrosoguanidine solution, at the same time they were redefined on the diet containing 5% NaCl. After this period expired, the animals were fed with standard diet till the end of the 24th week. The gastric mucosa cells were extracted at the end of the 4th, 6th, 8th, 10th, 12th, 18th and 24th week and underwent biochemical examinations. It was established the elevated phospho-c-Jun content, ornithine decarboxylase and inducible nitric oxide synthase activities from 6th to 24th week of gastric cancer development compared to the control references. The increasing of ornithine decarboxylase activity could probably be caused by the growth of phospho- c-Jun, it is also belonging to an ornithine decarboxylase transactivation effects. Thus, it was shown that the increase of ornithine decarboxylase and inducible nitric oxide synthase activities, phospho-c-Jun and nitrite-ions accumulation in gastric mucosa epithelial cells were associated with the gastric malignant progression. The complex relationships between the examined enzymes and transcription activator that pointed to an aggravation of pathological disturbances due to reciprocal action between ornithine decarboxylase and c-Junand nitric oxide synthase participation.

10.7603/s40730-016-0017-5

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Endotoxication indicators in the blood of patients with ischemic stroke complicated by type two diabetes mellitus

Bulletin of Taras Shevchenko National University of Kyiv Series Problems of Physiological Functions Regulation (2016)

T. Tsarenko L. Gayda Olha Kravchenko

Diabetes is one of the generally accepted factors of acute cerebral blood flow disorders. Numerous studies have shown involvement of many factors in the pathogenesis of diabetic cerebral disorders, the most important of which are the metabolic changes, including endotoxication. The aim of this study was to measure the endotoxication parameters and total protein and albumin contents in the blood of patients with ischemic stroke and patients with stroke complicated by type 2 diabetes. Also the average sex, age of the patients, body mass index (BMI) and blood glucose and lipoproteins levels, different disease complication presents and NIH Stroke Scale with Barthel index were determined. The investigation had shown that patients with ischemic stroke, including complicated by insulin independent diabetes, were characterized by hypoproteinemia with the absence of marked changes of albumin content. The middle mass molecule (MSM) and oligopeptide contents revealed the presence of endogenous intoxication. These parameters, exceeding the control by 1.5 times, were high independently of hyperglycemia presence. The results also allowed to establish differences in BMI, hyperlipoproteinemia and increasing of NIHSS in patients with ischemic stroke and diabetes mellitus type 2 comparing with stroke alone. Thus, the development of ischemic stroke separately and under conditions of type II diabetes was characterized by increasing rates of endotoxication (middle mass molecule and oligopeptides contents) in the blood of patients, that with hypoproteinemia and hyperlipidemia may cause the secondary pathobiochemical changes in the head brain cells and mediate the negative effects of acute disorders of cerebral blood circulation.

Hypoproteinemia

Stroke

Hyperlipidemia

NEFA

10.17721/2616_6410.2016.21.57-61

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DISTRIBUTION OF SERINE PROTEASES IN BLOOD PLASMA AND PANCREAS IN CHRONIC PANCREATITIS AND ONCOPATHOLOGY

Fìzìologìčnij žurnal (2022)

Tatyana Synelnyk Olha Kravchenko O.S. Kostiuk О. Savchuk С. А. Суходоля

The aim of our study was to evaluate the trypsin-like serine proteases (TLPs) distribution between systemic circulation and pancreatic tissue and to investigate the peculiarities of their involvement in the extracellular matrix components degradation in patients with pancreatic pathologies with electrophoretic analysis methods using. Тhe Khmelnitsky Regional Clinical Hospital patients aged 28-89 were selected for this study: 20 people with chronic pancreatitis (group CP); 20 people with pancreatic cancer (group PC); 20 conditionally healthy persons (control). Blood plasma samples and pancreatic tissue homogenates were obtained from all the patients, from which the TLPs fractions were subsequently obtained by the affinity chromatography method. The study showed that TLPs content in the blood plasma of patients with pancreatic pathologies is higher, and in tissue homogenates is lower relative to the values of the corresponding indicators in the control. Disk-electrophoresis using showed that TLPs fractions obtained from the blood plasma of patients of all studied groups contain a lot of high molecular weight (HMW) proteins, while TLPs from the pancreatic tissue homogenates of patients with pancreatic pathologies mainly consists of low molecular weight (LMW) proteins. Enzyme-electrophoresis results showed that all TLPs fractions include enzymes with fibrinogenolytic, gelatinolytic and collagenolytic activity. In plasma, the first were represented by medium molecular weight (MMW) proteins, and the last two groups included a lot of HMW proteins as well as proteins with very high molecular weight. In homogenates, fibrinogenolytic activity was characteristic for LMW proteins only, whereas gelatinases and collagenases were represented by both MMW and LMW proteins. Our results indicate the differences in the TLPs fractions components obtained from blood plasma and pancreatic tissue of patients with investigated pathologies, as well as significant distinctions in the processes of extracellular matrix remodeling under СР and РС.

Blood plasma

10.15407/fz68.06.031

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Citations (4)

The blood coagulation tests from ischemic stroke patients with or without type 2 diabetes mellitus

Biomedical Research - India (2018)

Olha Kravchenko Volodymyr S. Melnyk T. Tsarenko Oleksandra Kostiuk Tetyana Halenova

Objective: We determined the plasma protein C and coagulation factor X activities, prothrombin, fibrinogen and soluble fibrin monomer complex (SFMC) content and performed the haemostatic screening coagulation time tests under acute ischemic stroke patients with or without type 2 diabetes mellitus, as well as evaluated the significance of biochemical haemostatic markers as predictors of mortality in stroke regardless of diabetes presence.Methods: The baseline data were collected from 87 patients during the admission.Neurological disturbances were assessed using the NIH stroke scale.The functional outcome was estimated using Barthel index.All patients underwent fibrinogen and SFMC (gravimetric methods), prothrombin level (ELISA), plasma protein C and coagulation factor X activity assessment, haemostatic screening coagulation time tests with coagulation analyzer, glucose and glycosylated hemoglobin content and BMI (body mass index) measurements.Results: The conducted research had established the changes of fibrinogen and SFMC levels in both investigated patient groups comparing to the control.The protein C activity was found to be significantly decreased in blood of patients with ischemic stroke with and without diabetes.There were differences in factor X activity change in patients with stroke only comparing with patients with diabetes and stroke and high level of this parameter as well as the increase in SFMC can be regarded as death predictors of stroke independently of diabetes mellitus presence.Conclusion: Among ischemic stroke patients with type 2 diabetes mellitus the differences were more significant for all Time tests of the coagulation cascade, but deviations of haemostatic biochemical markers were more pronounced in ischemic stroke patients without diabetes mellitus.

Stroke

Coagulation testing

10.4066/biomedicalresearch.29-18-332

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FIBRINOGEN AND ITS COMPLEX PLASMA CONTENT UNDER ISCEMIC STROKE WITH OR WITHOUT DIABETES MELLITUS

Biological Markers in Fundamental and Clinical Medicine (collection of abstracts) (2018)

Olha Kravchenko

FIBRINOGEN AND ITS COMPLEX PLASMA CONTENT UNDER ISCEMIC STROKE WITH OR WITHOUT DIABETES MELLITUS

Stroke

10.29256/v.02.01.2018.escbm11

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The phospho-c-Jun (Ser63) content in mucosa cells under experimental development of gastric cancer.

PubMed (2014)

Tymoshenko Mo Olha Kravchenko Sokur Ov Л. І. Остапченко

The aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats.Gastric carcinoge-nesis was initiated in 80 white male rats by 10-week replacement of drinking water with 0.01% solution of N-methyl-N´-nitro-N-nitrosoguanidine, at the same time they were redefined on diet containing 5% NaCl. Then the animals were fed with standard vivarium diet till the end of 24(th) week. The gastric mucosa cells were examined at the end of 4(th), 6(th), 8(th), 10(th), 12(th), 18(th), and 24(th) weeks. Sandwich ELISA method was used to determine the content of phospho-c-Jun. The contents of GSH and GSSG were analyzed by spectrofluorymetric method with the use of orthophthalic aldehyde.At the end of 6(th), 8(th), 10(th) weeks of MNNG and NaCl treatment the gastric mucosa cells were characterized by 4-, 6.3-, 1.9-fold higher content of phospho-c-Jun compared to the control, respectively, and 12, 18 and 24 weeks there was registered a stable increase of phospho-c-Jun content on the average at 3.6-fold compared to control values. Starting from 6(th) week of gastric cancer development an average decrease of GSH/GSSG was 3.4-fold compared with the control.During gastric carcinogenesis there was registered the decrease of GSH/GSSG ratio and increased level of phosphorylated c-Jun what points on MAP-kinase cascade activation in prooxidant conditions.

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