Richard D. Cuthbert

Agriculture and Agri-Food Canada

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R. E. Knox

Swift Current Research and Development Centre

R. M. DePauw

Agriculture and Agri-Food Canada

Yuefeng Ruan

Agriculture and Agri-Food Canada

Curtis Pozniak

University of Saskatchewan

Asheesh K. Singh

Iowa State University

Heather Campbell

Harper Adams University

F. R. Clarke

Agriculture and Agri-Food Canada

Brent McCallum

Agriculture and Agri-Food Canada

J. M. Clarke

University of Saskatchewan

Firdissa E. Bokore

Agriculture and Agri-Food Canada

Cooperative Institutions

Agriculture and Agri-Food Canada

125

University of Saskatchewan

Swift Current Research and Development Centre

University of Manitoba

National Research Council Canada

Saskatchewan Research Council (Canada)

Ottawa Research and Development Centre

Kansas State University

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Université Paris-Saclay

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AAC Marchwell durum wheat

Canadian Journal of Plant Science (2014)

Arti Singh J. M. Clarke R. E. Knox R. M. DePauw I.L. Wise

Singh, A. K, Clarke, J. M., Knox, R. E., DePauw, R. M., Wise, I., Thomas, J., McCaig, T. N., Cuthbert, R. D., Clarke, F. R. and Fernandez, M.R. 2015. AAC Marchwell durum wheat. Can. J. Plant Sci. 95: 189–195. AAC Marchwell durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.] is adapted to the durum production area of th Canadian prairies. AAC Marchwell is the first durum genotype registered for commercial production in Canada with the Sm1 gene for antibiosis-based resistance to orange wheat blossom midge [Sitodiplosis modellana (Gehin)]. It combines high grain yield, grain protein concentration, yellow pigment, test weight, and low grain cadmium concentration. AAC Marchwell has similar straw strength, plant height, and days to maturity as Strongfield. AAC Marchwell is resistant to leaf rust, stem rust, stripe rust, common bunt, loose smut, and common root rot. AAC Marchwell has end use quality suitable for the Canada Western Amber Durum class.

Triticale

Triticum turgidum

Test weight

Antibiosis

10.4141/cjps-2014-223

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Citations (6)

A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel

Plant Methods (2024)

Jatinder S. Sangha Brad Meyer Yuefeng Ruan Richard D. Cuthbert R. E. Knox

Grain Quality

Kernel (algebra)

10.1186/s13007-024-01194-3

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Citations (0)

Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars

Frontiers in Plant Science (2023)

Firdissa E. Bokore Richard D. Cuthbert R. E. Knox Colin W. Hiebert Curtis Pozniak

The Canada Western Red Spring wheat (Triticum aestivum L.) cultivars AAC Concord, AAC Prevail, CDC Hughes, Lillian, Glenlea, and elite line BW961 express a spectrum of resistance to leaf rust caused by Puccinia triticina Eriks. This study aimed to identify and map the leaf rust resistance of the cultivars using three doubled haploid populations, AAC Prevail/BW961 (PB), CDC Hughes/AAC Concord (HC), and Lillian/Glenlea (LG). The populations were evaluated for seedling resistance in the greenhouse and adult plant disease response in the field at Morden, MB for 3 years and genotyped with the 90K wheat Infinium iSelect SNP array. Genetic maps were constructed to perform QTL analysis on the seedling and field leaf rust data. A total of three field leaf rust resistance QTL segregated in the PB population, five in the HC, and six in the LG population. In the PB population, BW961 contributed two QTL on chromosomes 2DS and 7DS, and AAC Prevail contributed a QTL on 4AL consistent across trials. Of the five QTL in HC, AAC Concord contributed two QTL on 4AL and 7AL consistent across trials and a QTL on 3DL.1 that provided seedling resistance only. CDC Hughes contributed two QTL on 1DS and 3DL.2. Lillian contributed four QTL significant in at least two of the three trials on 2BS, 4AL, 5AL, and 7AL, and Glenlea two QTL on 4BL and 7BL. The 1DS QTL from CDC Hughes, the 2DS from BW961, the 4AL from the AAC Prevail, AAC Concord, and Lillian, and the 7AL from AAC Concord and Lillian conferred seedling leaf rust resistance. The QTL on 4AL corresponded with Lr30 and was the same across cultivars AAC Prevail, AAC Concord, and Lillian, whereas the 7AL corresponding with LrCen was coincident between AAC Concord and Lillian. The 7DS and 2DS QTL in BW961 corresponded with Lr34 and Lr2a, respectively, and the 1DS QTL in CDC Hughes with Lr21. The QTL identified on 5AL could represent a novel gene. The results of this study will widen our knowledge of leaf rust resistance genes in Canadian wheat and their utilization in resistance breeding.

Rust (programming language)

Doubled haploidy

Marker-Assisted Selection

10.3389/fpls.2023.1130768

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Citations (7)

Effectiveness of multigenerational transfer of Sumai 3 Fusarium head blight resistance in hard red spring wheat breeding populations

Canadian Journal of Plant Science (2019)

Samia Berraies R. E. Knox R. M. DePauw F. R. Clarke Adam R. Martin

Several quantitative trait loci (QTL) have been identified for Fusarium head blight (FHB) resistance in the cultivar Sumai 3. Wheat breeders need to know which Sumai 3 loci are present in derived lines used as parents for effective marker-assisted selection for genetic improvement. This study was conducted to identify the loci in Sumai 3 derived parents that contribute FHB resistance in breeding populations. Three doubled haploid (DH) populations utilizing Sumai 3 derived parents, ND3085, ND744, and Alsen, were evaluated during 2007 and 2008 in FHB nurseries near Carman, MB, Ottawa, ON and Charlottetown, PE. The percentage of incidence, severity, Fusarium-damaged kernels (FDK), and deoxynivalenol (DON) accumulation were measured, and FHB index calculated. DNA markers at six FHB resistance loci detected in Sumai 3 were evaluated on the populations. For each trait, a t test was applied to means of observations pooled by parental type of each marker to determine which loci contributed to resistance. The alleles at 3BS and 5AS most frequently contributed to Type I and Type II FHB resistance, as well as to reduced FDK and DON in all three populations. Markers revealed resistance on 3BS and 5AS in Alsen, ND3085, and ND744, on 3BSc, 4D, and 6BS in ND744, on 4D in ND3085, and on 6BS in Alsen. In some environments, the susceptible parent Infinity contributed minor QTL on 2D, 3BSc, and 6BS. Likewise, Helios contributed minor QTL on 5AS and 6BS.

Trait

10.1139/cjps-2019-0066

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Citations (3)

High Density Mapping of Quantitative Trait Loci Conferring Gluten Strength in Canadian Durum Wheat

Frontiers in Plant Science (2020)

Yuefeng Ruan Bianyun Yu R. E. Knox Asheesh K. Singh R. M. DePauw

Gluten strength is one of the factors that determines the end product quality of durum wheat and is an important breeding target for this crop. To characterize the quantitative trait loci (QTL) controlling gluten strength in Canadian durum wheat cultivars, a population of 162 doubled haploid (DH) lines segregating for gluten strength and derived from cv. Strongfield × cv. Pelissier was used in this study. The DH lines, parents and checks were grown in three years and two seeding dates in each year and gluten strength of grain samples was measured by SDS-sedimentation volume (SV). With a genetic map created by genotyping the DH lines using the Illumina Infinium iSelect Wheat 90K SNP (single nucleotide polymorphism) chip, QTL contributing to gluten strength were detected on chromosome 1A, 1B, 2B and 3A. Two major and stable QTL detected on chromosome 1B (QGlu.spa-1B.1) and 1A (QGlu.spa-1A) explaining 25.4- 40.1% and 13.7-18.7% of the gluten strength variability respectively were consistently detected over three years, with the trait increasing alleles derived from Strongfield. Putative candidate genes underlying the QTL were identified. Two minor QTL (QGlu.spa-3A.1 and QGlu.spa-3A.2) with the trait increasing allele derived from Pelissier were mapped on chromosome 3A explaining up to 8.9% of the phenotypic variance; another three minor QTL (QGlu.spa-2B.1, QGlu.spa-2B.2 and QGlu.spa-2B.3) located on chromosome 2B explained up to 8.7% of the phenotypic variance with the trait increasing allele derived from Pelissier. Multi-environment analysis revealed genetic (QTL) × environment interaction due to the difference of effect in magnitude rather than the direction of the QTL. Eleven pairs of digenic epistatic QTL were identified, with an epistatic effect between the two major QTL of QGlu.spa-1A and QGlu.spa-1B.1detected in 4 out 6 environments. SNPs flanking the QTL interval can be converted to Kompetitive Allele Specific PCR (KASP) markers to be deployed in marker-assisted breeding to increase the efficiency and accuracy of phenotypic selection for gluten strength in durum wheat. The QTL that were expressed consistently across environments are of great importance to maintain the gluten strength of Canadian durum wheat to current market standards during genetic improvement for other traits.

Doubled haploidy

Candidate gene

10.3389/fpls.2020.00170

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Citations (18)

Publisher Correction: Exome sequencing highlights the role of wild-relative introgression in shaping the adaptive landscape of the wheat genome

Nature Genetics (2019)

Fei He Raj Pasam Fan Shi Surya Kant Gabriel Keeble‐Gagnère

Introgression

Exome

10.1038/s41588-019-0463-2

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Citations (10)

Conditional Mapping Identified Quantitative Trait Loci for Grain Protein Concentration Expressing Independently of Grain Yield in Canadian Durum Wheat

Frontiers in Plant Science (2021)

Yuefeng Ruan Bianyun Yu R. E. Knox Wentao Zhang Asheesh K. Singh

Grain protein concentration (GPC) is an important trait in durum cultivar development as a major determinant of the nutritional value of grain and end-use product quality. However, it is challenging to simultaneously select both GPC and grain yield (GY) due to the negative correlation between them. To characterize quantitative trait loci (QTL) for GPC and understand the genetic relationship between GPC and GY in Canadian durum wheat, we performed both traditional and conditional QTL mapping using a doubled haploid (DH) population of 162 lines derived from Pelissier × Strongfield. The population was grown in the field over 5 years and GPC was measured. QTL contributing to GPC were detected on chromosome 1B, 2B, 3A, 5B, 7A, and 7B using traditional mapping. One major QTL on 3A ( QGpc.spa-3A.3 ) was consistently detected over 3 years accounting for 9.4–18.1% of the phenotypic variance, with the favorable allele derived from Pelissier. Another major QTL on 7A ( QGpc.spa-7A ) detected in 3 years explained 6.9–14.8% of the phenotypic variance, with the beneficial allele derived from Strongfield. Comparison of the QTL described here with the results previously reported led to the identification of one novel major QTL on 3A ( QGpc.spa-3A.3 ) and five novel minor QTL on 1B, 2B and 3A. Four QTL were common between traditional and conditional mapping, with QGpc.spa-3A.3 and QGpc.spa-7A detected in multiple environments. The QTL identified by conditional mapping were independent or partially independent of GY, making them of great importance for development of high GPC and high yielding durum.

Doubled haploidy

Family-based QTL mapping

Trait

10.3389/fpls.2021.642955

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Citations (9)

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

Euphytica (2015)

Sachin Kumar R. E. Knox F. R. Clarke Curtis Pozniak R. M. DePauw

Abstract Pre-harvest sprouting in spring wheat causes significant financial loss to growers throughout the world and sprouting damage can be reduced by growing resistant genotypes. Several genetic factors, especially those related to seed dormancy, are involved in the control of pre-harvest sprouting resistance. The objective of this study was to identify quantitative trait loci (QTL) influencing pre-harvest sprouting resistance from multiple measures of dormancy at multiple germination intervals on seed harvested across multiple environments. A doubled haploid mapping population of 91 individuals derived from a cross of two Canadian white-seeded spring wheat genotypes, SC8021-V2 (pre-harvest sprouting resistant) and AC Karma (moderately susceptible to pre-harvest sprouting) was used for QTL mapping. Daily germination counts were analysed using germination index, germination resistance and percent germination at intervals of 3, 5, 7, 10, 14 and 21 days from spike samples collected from six field and one greenhouse environments in Saskatchewan, Canada. Continuous frequency distributions at certain measure-durations indicated genetic complexity of dormancy segregation in the SC8021-V2/AC Karma cross. Composite interval mapping detected significant ( p ≤ 0.05) QTL associated with resistance to pre-harvest sprouting on all 21 wheat chromosomes. Of the 26 total QTL, six were novel and the rest were detected either at the same marker or overlapping a marker interval reported in other studies. QTL expressed consistently for germination index, germination resistance and percent germination at different germination durations on chromosomes 2B, 4A, 5D and 6D. QTL identified on homoeologous chromosomes 4A, 4B and 4D with chromosome specific molecular variants of SSR marker wmc617 suggest a conserved region for controlling dormancy on group four. The majority of QTL mapped in regions known to contain factors affecting different components of pre-harvest sprouting resistance like seed dormancy, seed coat colour, ABA responsiveness and alpha-amylase activity. This study demonstrated that using multiple measures of seed dormancy at multiple intervals of germination enhanced identification of QTL affecting dormancy in white-seeded hexaploid wheat.

Sprouting

10.1007/s10681-015-1460-x

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Citations (30)

Genetic Characterization of Multiple Components Contributing to Fusarium Head Blight Resistance of FL62R1, a Canadian Bread Wheat Developed Using Systemic Breeding

Frontiers in Plant Science (2020)

Wentao Zhang Kerry Boyle Anita L. Brûlé‐Babel George Fedak Peng Gao

Fusarium head blight (FHB) is a devastating fungal disease of small-grain cereals that results in severe yield and quality losses. FHB resistance is controlled by resistance components including incidence, field severity, index, Fusarium Damaged Kernels (FDKs), and the accumulation of the mycotoxin DON. Resistance conferred by each of these components is partial, and must be combined to achieve resistance sufficient to protect wheat from yield losses. In this study, two biparental mapping populations were analyzed in Canadian FHB nurseries and QTL mapped for the traits listed above. Nine genomic loci, on 2AS, 2BS, 3BS, 4AS, 4AL, 4BS, 5AS, 5AL and 5BL, were enriched for the majority of the QTL controlling FHB resistance. The previously validated FHB resistance QTL on 3BS and 5AS, affected resistance to severity, FDK and DON in these populations. The remaining seven genomic loci co-localize with flowering time and/or plant height QTL. The QTL on 4B was a major contributor to all field resistance traits and plant height in the field. QTL on 4AL showed contrasting effects for FHB resistance between Eastern and Western Canada, indicating a local adapted resistance to FHB. Results presented here provide insight into the genetic architecture underlying these resistant components and insight into how FHB resistance in wheat is controlled by a complex network of interactions between genes controlling flowering time, plant height, local adaption and FHB resistance components.

Genetic architecture

10.3389/fpls.2020.580833

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Citations (13)

Genetic characterization of multiple components contributing to fusarium head blight resistance of FL62R1, a Canadian bread wheat developed using systemic breeding

Figshare (2020)

Wentao Zhang Kerry Boyle Anita L. Brûlé‐Babel George Fedak Peng Gao

In this study, nine genomic loci were identified for controlling FHB resistance. SNP markers associated with detected QTL were presented by these four datasets listed here.
File 1. Sequence information of SNPs for FHB resistance QTLFile 2. the genetic map of FL62R1/Stettler Population
File 3. The genetic map of the FL62R1/Muchmore Population
File 4. Details of detected QTL for FHB resistance

10.6084/m9.figshare.12948263

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Citations (1)