OBJECTIVE Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mrel11 fluctuations after HBV infection which might be eventually contributed to cell transformation. METHODS Western Blot was monitored to detect the expression of Mre11, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks. RESULTS Mre11 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mre11 knockdown caused DNA instability. CONCLUSION Mre11 expression downregulation contributed at least partially to cell transformation caused by HBV infection.
Error calibration is one of the most important factors to realize the quadri-wave lateral shearing interferometer (QWLSI) with high accuracy. The misalignment errors of QWLSI, such as the tilt of grating and the tilt of charge coupled device (CCD), will affect the measurement accuracy. The astigmatism errors induced by the tilt of grating and CCD during the alignment process of QWLSI, which are neglected in previous studies, are analyzed and presented in analytical expressions in this paper. Firstly, the additional phase difference in X and Y directions induced by the tilt of grating and CCD are analyzed using the optical wave interference theory. Representing the phase difference in the two directions and the test wavefront with the combinations of Zernike polynomials respectively, we further obtain the analytical expressions between the Zernike coefficients of the phase difference and the Zernike coefficients of the test wavefront, according to the wavefront reconstruction theory. Then the analytical expressions for the measurement errors induced by the tilt of grating and CCD, which are mainly astigmatism, can be obtained. The analytical results show that the misalignment induced astigmatism errors are inversely proportional to the shearing ratio and proportional to the tilt angle of grating and CCD. The alignment experiment of a home-made QWLSI under null test condition is conducted to verify the correctness of the theoretical analysis. With different shearing ratios for the QWLSI, the astigmatism errors, which are induced by the tilt of grating in experimental results, are consistent with the theoretical analysis results. This paper can provide technical support for the alignment of QWLSI with small shearing ratio and high precision.
A new method, to the best of our knowledge, based on double-slit (DS) interference is proposed to accurately estimate the shear ratio of the system, with plane wave or spherical wave incidence. Existing shear ratio calibration methods, designed primarily for lateral shearing interferometry (LSI) with plane wave incidence, are not applicable to LSIs directly testing divergent or convergent spherical waves. Equations for calculating the shear ratio using the fringe spacing of the DS interferogram and the NA of the incident spherical wave are derived in this paper. The simulation result shows that the relative error of the shear ratio value is about 0.3%, when the shear ratio is 0.1. In the experiment, the quadriwave LSI is designed with a plug-in feature. The shear ratio at integer multiples of 1/6 Talbot distance from the modified Hartmann mask was calibrated using a DS, and the results were in good agreement with theoretical values, confirming the accuracy of the method. Subsequently, with the assistance of an inductance micrometer, the shear ratio was calibrated at intervals of 0.5 mm, and the results closely matched the theoretical variation of the shear ratio caused by displacement, confirming the high precision of the method.
Background Our prior study in Han Chinese women has shown that women with a history of childhood sexual abuse (CSA) are at increased risk for developing major depression (MD). Would this relationship be found in our whole data set? Method Three levels of CSA (non-genital, genital, and intercourse) were assessed by self-report in two groups of Han Chinese women: 6017 clinically ascertained with recurrent MD and 5983 matched controls. Diagnostic and other risk factor information was assessed at personal interview. Odds ratios (ORs) were calculated by logistic regression. Results We confirmed earlier results by replicating prior analyses in 3,950 new recurrent MD cases. There were no significant differences between the two data sets. Any form of CSA was significantly associated with recurrent MD (OR 4.06, 95% confidence interval (CI) [3.19–5.24]). This association strengthened with increasing CSA severity: non-genital (OR 2.21, 95% CI 1.58–3.15), genital (OR 5.24, 95% CI 3.52–8.15) and intercourse (OR 10.65, 95% CI 5.56–23.71). Among the depressed women, those with CSA had an earlier age of onset, longer depressive episodes. Recurrent MD patients those with CSA had an increased risk for dysthymia (OR 1.60, 95%CI 1.11–2.27) and phobia (OR 1.41, 95%CI 1.09–1.80). Any form of CSA was significantly associated with suicidal ideation or attempt (OR 1.50, 95% CI 1.20–1.89) and feelings of worthlessness or guilt (OR 1.41, 95% CI 1.02–2.02). Intercourse (OR 3.47, 95%CI 1.66–8.22), use of force and threats (OR 1.95, 95%CI 1.05–3.82) and how strongly the victims were affected at the time (OR 1.39, 95%CI 1.20–1.64) were significantly associated with recurrent MD. Conclusions In Chinese women CSA is strongly associated with recurrent MD and this association increases with greater severity of CSA. Depressed women with CSA have some specific clinical traits. Some features of CSA were associated with greater likelihood of developing recurrent MD.
To address the problems present in the treatment of uveitis.A retrospective analysis was made on 154 patients (268 eyes) with different entities of uveitis, coming from all over China and referring to Zhongshan ophthalmic center from April to October of 2000. The data of these patients, especially with respect to the usage of corticosteroids and antibiotics in the referred hospitals, were analyzed.Corticosteroids drops, subconjunctival or retrobulbar injection and systemic corticosteroids were respectively used in 90.3% and 84.0% of these eyes, and 92.9% of these cases. Intravenous injection of corticosteroids was rather popular, accounting for 85.3% of the cases who were treated with systemic corticosteroids. Improperly aggressive treatment with corticosteroids or undue application of their eye drops was equally found in patients from county-, city- and province-level hospitals, especially in Behcet's disease, anterior uveitis and Fuchs heterochromic uveitis. Antibiotics drops, subconjunctival injection and systemic administration of antibiotics were respectively used in 97.0% and 71.6% of these eyes and 83.8% of these patients. Immunosuppressive drugs, other than the corticosteroids, were used in 6 patients only.Corticosteroids should be used in proper methods, dosage and duration based on the entities and severity of uveitis. It is not necessary to treat noninfectious uveitis with antibiotics.
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