von Eisenhart-Rothe B

Universität Hamburg

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

[Oxygen affinity and regeneration capacity of SAG-M and PAGGS-M stored erythrocytes].

PubMed (1992)

von Eisenhart-Rothe B T Dominka W. Rödiger Ingrid Ganschow B Ivens

In order to estimate the leftward shift of the oxygen dissociation curve of hemoglobin following storage of red blood cells (rbc) in the additive solutions SAG-M and PAGGS-M, respectively, we performed blood gas analyses after equilibrating the cells with a gas mixture containing 4% of O2 and 5% of CO2 at pH 7.4 and 37 degrees C. Additionally, we took advantage of these nearly physiological conditions to measure the recovery of the hemoglobin function in vitro. We observed a good correlation between 2,3-BPG and p50 (i.e. the oxygen tension, at which hemoglobin is half-saturated with oxygen). Within the first 3 weeks of storage, the 2,3-BPG content fell to one fifth of its original value, whereas the p50 declined from 26.6 to about 20 mm Hg. Compared to fresh cells, rbc stored for longer than 3 weeks will thus deliver 30% less oxygen to the myocardium. The rbc's ability to restore these parameters remained unchanged throughout 7 weeks of storage.

Oxygen tension

Oxygen transport

Source

Cite

Citations (1)

[The organizational preparation of blood transfusion with consideration for the new formation of transfusion guidelines].

PubMed (1979)

von Eisenhart-Rothe B

Source

Cite

Citations (1)

[Rapid and sensitive determination of the adenylate energy charge in stored erythrocytes using high pressure liquid chromatography].

PubMed (1990)

Walter Sibrowski K Lüdemann T Dominka von Eisenhart-Rothe B P. Kühnl

Using reversed-phase high-performance liquid chromatography (HPLC), we developed a fast method for the determination of the adenine-nucleotides ATP, ADP, and AMP in human red blood cells (RBC). The method is sensitive (10(-9)-10(-10) M), specific (greater than 95% peak-homogeneity), and the results are well reproducible. The three substances are assayed in a single step, which is essential for the exact determination of the adenylate-energy-charge (AEC). We applied the technique to fresh and stored RBC's. After six weeks' storage in PAGGS-sorbitol we found only a slight decrease in ATP content, but marked increases in ADP and AMP contents. Accordingly, there was a decrease in the AEC, whereas the total nucleotide pool (TNP) did not change significantly. As AEC and TNP reflect the state of the cellular energy metabolism, they are related to the viability of stored RBC. Thus, we consider the described method to be helpful for the quality control of RBC stored in new or modified media.

Energy charge

Adenine nucleotide

Source

Cite

Citations (0)

[Characterization of damage caused by storage of preserved erythrocytes using specific erythrocyte aging parameters].

PubMed (1988)

Walter Sibrowski T Dominka von Eisenhart-Rothe B

Blood preservation

Source

Cite

Citations (0)

[Preventive-medicine screening methods in the examination of blood donors].

PubMed (1977)

H Busch von Eisenhart-Rothe B J A Kohler

From a blood transfusion service with ca. 7000 donors a year, there was a loss of donors whose blood, having undergone medical tests, was found to be unsuitable. Details of all such donors were carefully documented. The results were ascertained by attentive control of the health of the donors which, in part, exceeded the minimum requirements laid down by the existing regulations. The relatively high loss of donors and the kind of disease underline the importance of these control checks, as an aspect of preventive medicine. This is of particular importance with regard to the results of so-called "new donors", who register for the first time. They had to undergo examination, and were only allowed to give blood when all the results of the tests had been submitted. The question then arises as to whether the regulations for the differing minimum requirements for the examination of "occasional donors" and "regular donors" can be maintained. A reduction of the expenditure on the present regulation examination of donors is not advocated because of both responsibility towards the blood donors, and in view of the increasing significance, to the medical care of patients, of the proximity of a clinic to an expedient transfusion service.

Transfusion medicine

Medical care

Health examination

Preventive healthcare

Source

Cite

Citations (0)

[Comparison of erythrocyte concentrations of ATP, ADP, AMP and 2,3-BPG in storage of five different additive solutions and in serum].

PubMed (1992)

von Eisenhart-Rothe B I Ganschow T Dominka P. Kühnl

In this study, we compared the ability of serum and of 5 additive solutions for the liquid storage of red blood cells to preserve the cellular contents of 2,3-BPG and the adenylate nucleotides ATP, ADP and AMP. As to the adenylates, PAGGS-M showed the best results. However, the difference to SAG-M was significant only after 7 weeks. On the other hand, serum appears to be the best medium for the conservation of 2,3-BPG. Between the 1st and 3rd week of storage, the decrease of 2,3-BPG was somewhat slower in PAGGS-M and SAG-M compared with the other 3 additive solutions.

Adenine nucleotide

Source

Cite

Citations (2)

["Old new" problem of the lues risk in transfusion medicine: the effect of the serum and ACD stabilizer at different temperatures on the infectiosity of Treponema pallidium (Nichols strain)].

PubMed (1979)

F. Müller von Eisenhart-Rothe B H. F. M. Busch

The portion of warm and fresh blood transfusions had increased obviously. This is accompanied by a decrease of the restrictive period of the former 72 and the presently 48 hours for blood units to be transfused. Therefore, the danger of a symphilis transmitted by transfusion is impending again. This is realized insufficiently and the syphilis may occur, if blood is drawn in the seronegative phase of the primary stage of syphilis and transfused within the restrictive period. Model trials on the infectivity of Treponema pallidum revealed that the syphilis cannot be transmitted by blood and its derivates drawn in the seronegative phase after storage at 4 degrees C for 24 hours. Therefore, the reduction of the restrictive period to 48 hours is justified.

Infectivity

Source

Cite

Citations (0)

[Leukocyte contamination in standard erythrocyte concentrates, prepared with the Biopack-U-bag and Biotrans separator].

PubMed (1990)

von Eisenhart-Rothe B E Spinger A. Frank P. Kühnl

Separator (oil production)

Source

Cite

Citations (0)

[Analysis of irregular antibodies at the department of transfusion medicine of the Hamburg-Eppendorf University Hospital 1984-1988].

PubMed (1990)

D Wittkopf Albert W. Grundmann Walter Sibrowski von Eisenhart-Rothe B P. Kühnl

In the University Hospital of Hamburg-Eppendorf (UKE) we performed approximately 80,000 screening tests for irregular RBC antibodies in the years 1984-1988. Among 1,815 (2.27%) positive tests, Rhesus and concomitant antibodies occurred in 0.77% (615/80,000), other clinically important antibodies in 0.37% (293 cases). We found significantly less Rh antibodies than in the previous period of the mid seventies (Rh prophylaxis, completely Rh-compatible transfusions).

Concomitant

Transfusion medicine

Source

Cite

Citations (0)

[The amino acid picture--studies on stored blood].

PubMed (1981)

Gerald Kessler G Schöntag von Eisenhart-Rothe B

In 6 blood units with increased duration of storage alterations of amino acid levels were measured. Total amino acid concentration rises to 27%, while the amount of essential amino acids increases to 11% The E/T-quotient decreases slightly; so does the quotient of essential to non essential amino acids. The changes of concentrations of amino acids in stored whole blood can deteriorate the metabolic situation of patients with disorders of liver or kidney function. These patients as well as intensive care patients, in whom insufficiency of liver and kidney often is seen, should get transfusions of packed cells instead of whole blood.

Source

Cite

Citations (0)