Marco Raimondo

Instituto Biofisika

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Co-Authors

Alberto Diaspro

University of Genoa

Mauro Robello

University of Genoa

Valeria Guarrasi

National Research Council

María Antonietta Germanà

University of Palermo

Simona Annunziata

University of Genoa

Fabio Librizzi

Instituto Biofisika

Maria Assunta Costa

Instituto Biofisika

Paola Ramoino

University of Genoa

Silvia Vilasi

Instituto Biofisika

Giacoma Cinzia Rappa

Instituto Biofisika

Cooperative Institutions

National Research Council

Instituto Biofisika

University of Palermo

University of Genoa

National Academies of Sciences, Engineering, and Medicine

Organizzazione per la Tutela Forestale, Ambientale e Agroalimentare

University of Palermo

University of Naples Federico II

Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy

Italian Institute of Technology

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Three-dimensional optical behaviour of a confocal microscope with single illumination and detection pinhole through imaging of subresolution beads

Microscopy Research and Technique (1999)

Alberto Diaspro Simona Annunziata Marco Raimondo Mauro Robello

Pinhole (optics)

10.1002/(sici)1097-0029(19990415)45:2<130::aid-jemt7>3.0.co;2-v

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Citations (24)

A single-pinhole confocal laser scanning microscope for 3-D imaging of biostructures

IEEE Engineering in Medicine and Biology Magazine (1999)

Alberto Diaspro Simona Annunziata Marco Raimondo Paola Ramoino Mauro Robello

Confocal laser scanning microscopy (CLSM) is a powerful three-dimensional (3-D) tool at the microscopic level, both for functional and for structural studies. The confocal microscope provides a practical and well-established method for obtaining microscopic volume imaging. It has become more and more evident that it permits one to obtain a large amount of information on biological systems in terms of their (slow) dynamics and 3-D structure. Moreover, when linked to bioimage-oriented interfaces, CLSM adds the ability to examine the spatial distribution and the temporal behaviour of specific cellular components to the already intriguing properties of confocal imaging. In this article, we discuss a new ultracompact confocal microscope based on a single-pinhole optical architecture.

Pinhole (optics)

Laser Scanning

Laser Microscopy

Optical sectioning

10.1109/51.775496

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Citations (16)

Functional imaging of living Paramecium by means of confocal and two-photon excitation fluorescence microscopy

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2002)

Alberto Diaspro Paola Fronte Marco Raimondo Marco Fato Gianluca De Leo

Confocal and Two-photon excitation laser scanning microscopy allow gathering three-dimensional and temporal information from biological systems exploiting fluorescence labeling and autofluorescence properties. In this work we study biological events linked to functionality in Paramecium primaurelia. The internalization of material in ciliated one-celled organisms (protozoa) occurs via different mechanisms, even if most of nutrients, particulate or not, is taken up by food vacuoles formed at the bottom of the oral cavity. The endocytosis of small-sized molecules occurs at the parasomal sacs, located next the ciliar basal bodies. Vital fluorescent dyes (BSA-FITC, WGA-FITC, dextran-Texas Red, cholesteryl-Bodipy) and autofluorescence were used to study formation, movement, and fusion of vesicles during endocytosis and phagocytosis of Paramecium primaurelia. By immobilizing living cells pulsed with food vacuole and endosome markers at successive times after chasing in unlabeled medium, the intracellular movement and fusion of food vacuoles and of endosomes were visualized. A temporal analysis of fluorescence images and the false-color technique were used. Starting from time series or 3D data sets composite images were generated by associating with each originally acquired image a different color corresponding to each sampling point in time and along the z-axis. Second Harmonic Generation Imaging attempts are also outlined.

Autofluorescence

Two-photon excitation microscopy

Live cell imaging

Fluorescence-lifetime imaging microscopy

10.1117/12.468356

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Citations (10)

Three-dimensional mapping of cholinergic molecules by confocal laser scanning microscopy in sea urchin larvae

Micron (2002)

Carla Falugi Alberto Diaspro C. Angelini M.L Pedrotti Marco Raimondo

Paracentrotus lividus

10.1016/s0968-4328(01)00018-x

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Citations (10)

Valorization of Apple Peels through the Study of the Effects on the Amyloid Aggregation Process of κ-Casein

Molecules (2021)

Valeria Guarrasi Giacoma Cinzia Rappa Maria Assunta Costa Fabio Librizzi Marco Raimondo

Waste valorization represents one of the main social challenges when promoting a circular economy and environmental sustainability. Here, we evaluated the effect of the polyphenols extracted from apple peels, normally disposed of as waste, on the amyloid aggregation process of κ-casein from bovine milk, a well-used amyloidogenic model system. The effect of the apple peel extract on protein aggregation was examined using a thioflavin T fluorescence assay, Congo red binding assay, circular dichroism, light scattering, and atomic force microscopy. We found that the phenolic extract from the peel of apples of the cultivar “Fuji”, cultivated in Sicily (Caltavuturo, Italy), inhibited κ-casein fibril formation in a dose-dependent way. In particular, we found that the extract significantly reduced the protein aggregation rate and inhibited the secondary structure reorganization that accompanies κ-casein amyloid formation. Protein-aggregated species resulting from the incubation of κ-casein in the presence of polyphenols under amyloid aggregation conditions were reduced in number and different in morphology.

Thioflavin

Congo red

Amyloid (mycology)

10.3390/molecules26082371

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Citations (4)

Caratterizzazione fitopastorale e produttiva di pascoli naturali ricadenti nella riserva naturale orientata di Ficuzza.

Luigi Stringi Alfonso Salvatore Frenda Vincenzo Ilardi Marco Raimondo M Raimondo

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Effect of Total Apple Polyphenols Extract as Inhibitors of Amyloid Protein Aggregation

Journal of Clinical Gastroenterology (2018)

Valeria Guarrasi C. Rappa M. Costa Fabio Librizzi Marco Raimondo

Amyloid (mycology)

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The precious content of the olive mill wastewater: the protective effect of the antioxidant fraction in cell cultures

CyTA - Journal of Food (2018)

Daniela Giacomazza Daniela D’Andrea Alessia Provenzano Pasquale Picone Fiorenza Provenzano

Oxidative stress and mitochondrial dysfunction play an important role in the onset of neurodegenerative diseases; thus, the antioxidant use could be a promising preventive or therapeutic intervention. The food and agricultural industries generate huge quantities of by-products rich in biophenolic compounds. If, on the one hand, they represent a serious environmental problem, on the other hand, are valuable natural sources of antioxidants. In the present work, the total phenolic content and the antioxidant power of the wastewater derived from the production of four Sicilian extra virgin olive oils are shown. Four biophenols, hydroxytyrosol and tyrosol alcohols and caffeic acid (CA) and p-coumaric acids, were identified and quantified and their antioxidant ability determined. Their effects in counteracting Abeta1-42 oligomer oxidative damage in LAN5 cell culture were investigated. Results indicated that CA was the most effective compound in counteracting the cell damages induced by Abeta1-42 oligomers.

Tyrosol

Hydroxytyrosol

10.1080/19476337.2018.1458752

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Citations (8)

Nuovi approcci alla definizione del rischio nelsistema urbano: l’entropia sistemica

Romano Fistola Marco Raimondo Rosa Anna La Rocca

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Different chloride electrochemical gradients across the plasma membrane in subcellular compartments of rat cerebellum granules

Neuroscience Letters (2005)