Optical imaging with high resolution is significant to reveal the functional activities of brain and the mechanism of disease, and has grown into a diverse field. The high-resolution multi-parameters optical imaging system which combines the laser speckle contrast imaging method and optical intrinsic signal imaging method can obtain more hemodynamic information in cortex simultaneously. However, most of current optical imaging systems use He-Ne laser and mercury xenon arc lamp as the light source. Meanwhile, the control unit of the system which includes a personal computer, is not portable. In this paper, we develop a multiple parameters hemodynamic imaging system based on ARM. To make the system more compact, three wavelengths light-emitting diode and laser diode as imaging illuminants were adopted. In addition, the real-time embedded operation system (μC/OS-II) and embedded Graphic User Interface (μC/GUI) were introduced. Animal experimental results also show that changes in oxyhemoglobin, deoxyhemoglobin, total hemoglobin concentration and cerebral blood flow during Cortical Spreading Depression can be simultaneously accessed using this optical imaging system based on embedded ARM.
Accumulating studies showed that microRNAs are maintaining a variety of important biological processes but the underlying mechanism in proliferation and tumourigenicity is unclear. In this study we show that miR-342 expression in bone marrow and patients' sera of childhood acute myeloid leukemia (AML) was both significantly higher than those in the corresponding normal controls. Functional assays demonstrated that forced expression of miR-342 significantly suppresses AML cell proliferation and G1/S transition of leukemia cells. Mechanistically, bioinformatics prediction and luciferase reporter assay identified N-a-acetyltransferase 10 protein (Naa10p) as a direct molecular target of miR-342, Naa10p siRNA significantly repressed cell proliferation and increased cell apoptosis. In conclusion, our study confirmed that miR-342/Naa10p plays key roles in AML progression, providing insights into underlying mechanisms of AML pathogenesis and also a potential therapeutic target for this malignancy.
There are abundant resources of wild lily in China. To achieve a sustainable use for these resources, a slow-growing preservation system should be well established. Therefore, to get an optimal storage condition, tube seedlings of Lilium davidii andLilium longiflorum were used as explants cultured on nine different media at 24 and -2°C separately, which included: 1/4 or full Murashige and Skoog medium (MS) with 3, 6 or 9% sucrose, 1/4 or 1/2 MS with 1.0 or 3.0 mg/L abscisic acid (ABA). During the storage, the spout growth, and multiplication rate were measured every month. Results show that the growth restriction degrees for both species were related to the four factors (MS content, the concentration of sucrose and ABA, temperature). The inhibitory effect was more obvious at -2°C. At 24°C, the medium should be replaced every three to four months; otherwise the contamination rate would rise with the extension of storage time. At -2°C, the 1/4 MS with 9% sucrose or 3.0 mg/L ABA (M3A) were more effective, especially the latter one. The M3A played the greatest inhibitory effect on height growth of the two lily species, on which both species had been conserved on the original medium for more than 15 months. The tube seedlings conserved for 15 months could turn to normal plantlets after re-growth for one month which showed no obvious difference in morphology. 87.5% plantlets planted in peat could survive. In conclusion, the promising conservation condition was M3A at -2°C which was suitable for mid-long term preservation of lily germplasm resources. Key words: Lily, wild species, slow-growth, conservation.
Background Kin17 is ubiquitously expressed at low levels in human tissue and participates in DNA replication, DNA repair and cell cycle control. Breast cancer cells are characterized by enabling replicative immortality and accumulated DNA damage. However, whether kin17 contributes to breast carcinogenesis remains unknown. Methodology/Principal Findings In this study, we show for the first time that kin17 is an important molecule related to breast cancer. Our results show that kin17 expression was markedly increased in clinical breast tumors and was associated with tumor grade, Ki-67 expression, p53 mutation status and progesterone receptor expression, which were assessed in a clinicopathologic characteristics review. Knockdown of kin17 inhibited DNA replication and repair, blocked cell cycle progression and inhibited anchorage-independent growth, while increasing sensitivity to chemotherapy in breast cancer cells. Moreover, kin17 silencing decreased EGF-stimulated cell growth. Furthermore, overexpression of kin17 promoted DNA replication and cell proliferation in MCF-10A. Conclusions/Significance Our findings indicate that up-regulation of kin17 is strongly associated with cellular proliferation, DNA replication, DNA damage response and breast cancer development. The increased level of kin17 was not only a consequence of immortalization but also associated with tumorigenesis. Therefore, kin17 could be a novel therapeutic target for inhibiting cell growth in breast cancer.
Abstract An efficient and highly selective reaction of 2‐(2‐bromophenyl)‐5,6,7,8‐tetrahydrobenzo [4,5] thieno[2,3‐ d ]pyrimidin‐4(3 H )‐one and terminal alkyne has been developed under palladium‐free conditions by CuI‐catalyzed sequential Sonogashira coupling reaction and 5‐exo‐dig hydroamination reaction. A series of 11‐arylidene‐2,3,4,11‐tetrahydrobenzo [4′,5′]thieno [2′,3′ : 4,5]pyrimido [2,1‐ a ]isoindol‐13(1 H )‐one derivatives with only the (E) ‐configuration were obtained.
This paper proposes a Grid-based Distributed Simulation Platform (GDSP). It is designed to break the limitation of legacy simulation supporting platform and to meet new requirements from large-scale distributed and complicated systems. Simulation services are encapsulated by using web services technology, and they are basic elements of GDSP. With grid technology, GDSP enables users to share, access, and operate remote resource transparently. It is also easier to organize and manage simulation resources. GDSP improves the capability of fault tolerance and provides mechanism of using simulation resources securely. The research result shows that through this management mechanism, multiple users can share and monitor RTI resources, and manage the simulation collaboratively and effectively. At the same time, the impact on real time of RTI caused by the system is minimum.
The high prevalence of chronic inflammatory diseases or autoimmune reactions is a major source of concern and affects the quality of life of patients. Chronic inflammatory or autoimmune diseases are associated with many diseases in humans, including asthma, rheumatoid arthritis, systemic lupus erythematosus, inflammatory bowel disease and cancer. Splenic tyrosine kinase (SYK) is a non-receptor tyrosine kinase that plays an important role in immune receptor signalling in immune and inflammatory responses.
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