Geisiane Alves Rocha

Universidade Federal de Goiás

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Marcos Gomes da Cunha

Universidade Federal de Goiás

Éd

Érico de Campos Dianese

Universidade Federal de Goiás

Vanessa Duarte Dias

Universidade Federal de Goiás

Renato Carrer Filho

Universidade Federal de Goiás

Renato de Carvalho Menezes

Universidade Federal de Goiás

Renata Maria de Oliveira

Universidade Federal de Minas Gerais

Renato Carrer-Filho

Universidade Federal de Goiás

Luciana Celeste Carneiro

Universidade Federal de Jataí

Cássio Jardim Tavares

Instituto Federal Goiano

Mariana Guimarães

Universidade Federal de Goiás

Cooperative Institutions

Universidade Federal de Goiás

Brazilian Agricultural Research Corporation

Universidade Federal de Viçosa

Universidade de São Paulo

Universidade Federal de Santa Maria

Universidade de Brasília

Instituto Federal Goiano

Universidade Federal do Rio Grande do Sul

Universidade Federal de Lavras

Faculdades Guarulhos

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SOLARIZAÇÃO DO SOLO ASSOCIADA À INCORPORAÇÃO DE MATERIAL ORGÂNICO NA REDUÇÃO DA VIABILIDADE DE ESCLERÓDIOS

Revista de Ciências Agroambientais (2016)

Geisiane Alves Rocha Luciana Celeste Carneiro

A aplicacao da solarizacao no Centro-Oeste brasileiro e potencialmente promissora, uma vez que o clima e quente na maior parte do ano e a incidencia de radiacao solar e alta, principalmente no periodo de estiagem. O objetivo deste trabalho foi avaliar o efeito da solarizacao do solo, com e sem material orgânico, na sobrevivencia dos esclerodios de Sclerotinia sclerotiorum e Sclerotium rolfsii . Solo previamente umedecido foi acondicionado em sacos de polietileno transparente sendo aplicados os seguintes tratamentos: “solarizado”, “solarizado + folhas de eucalipto”, “solarizado + folhas de repolho”, “solarizado + cama de frango”, “sacos abertos ao sol” e “sacos abertos a sombra”. Os tratamentos solarizados alcancaram maiores temperaturas em relacao aos nao solarizados. A sobrevivencia dos esclerodios foi menor depois de 14 dias de solarizacao para ambos os patogenos, destacando-se os tratamentos “solarizado + eucalipto” e “solarizado + repolho”, o qual se observou menor taxa de germinacao.

Sclerotinia Sclerotiorum

10.5327/rcaa.v14i1.836

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Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii

Ornamental Horticulture (2019)

Mariana Cunha Stutz Renato Carrer Filho Geisiane Alves Rocha Érico de Campos Dianese Marcos Gomes da Cunha

Abstract Zamioculcas zamiifolia (Araceae) is one of the most widely grown exotic species in Brazil as ornamental plants and in landscape design. Despite tolerating transport and being well adapted to low-light environments, this ornamental is attacked by different pathogens. Thus, the aim was to detect and identify the pathogen that causes stem rot in commercial Z. zamiifolia crops. Z. zamiifolia plants exhibiting stem rot symptoms were sent for phytosanitary diagnosis. In a culture medium, the fungal isolate obtained (SR-001) displayed the following morphological characteristics: cotton-like aerial mycelium, septate hyaline hyphae with no spore production, and the formation of small brown spherical sclerotia. To confirm pathogenicity, Z. zamiifolia plants were inoculated with the SR-001 isolate and, after fifteen days, the fungus was re-isolated when the same rot symptoms emerged. The SR-001 isolate was identified as Sclerotium rolfsii and its representative sequence was deposited in GenBank (Access MG694322). This fungal isolate has not been associated with diseases in Z. zamiifolia in Brazil, and this is the first report of the fungus infecting this ornamental plant species in a cultivated area.

Ornamental plant

Stem rot

Collar rot

10.1590/2447-536x.v25i4.2019

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Fusarium sacchari associated with stem rot in sweet corn in Brazil

Figshare (2023)

Renato Carrer Filho Mariana Guimarães Vanessa Pereira de Abreu Geisiane Alves Rocha Renato de Carvalho Menezes

ABSTRACT Sweet corn is susceptible to the attack of various pathogens that affect its metabolism and compromise its quality and production. This study aimed to identify the causal agent of stem rot in sweet corn plants under greenhouse conditions. The identity of the pathogenic isolate was confirmed by sequencing of internal transcribed spacer (ITS1/ITS4), beta-tubulin (BT2A/BT2B), calmodulin (CL1/CL2) and translation elongation factor 1α (TEF1). The morphological and sequencing characteristics showed that Fusarium sacchari, which belongs to the Fusarium fujikuroi complex, is directly associated with the symptoms observed in the field. This fungal isolate has never been associated with diseases in Zea mays (saccharata group) in Brazil; therefore, this is the first report of the fungus infecting sweet corn in a cultivated area.

Stem rot

10.6084/m9.figshare.22121277

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Characterization of a bean rugose mosaic virus isolate and search for virus resistance in a germplasm bank of Phaseolus vulgaris

Tropical Plant Pathology (2020)

Daniella Vieira Cândida Geisiane Alves Rocha Vanessa Duarte Dias Marcos Gomes da Cunha Josías C. Faria

Germ plasm

10.1007/s40858-019-00328-6

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An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

Rodriguésia (2020)

Geisiane Alves Rocha Vanessa Duarte Dias Renato Carrer-Filho Marcos Gomes da Cunha Érico de Campos Dianese

Abstract Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome. The methods tested consisted of: (i) TRIzol® reagent, (ii) TRIzol® reagent with modifications, (iii) CTAB buffer, and (iv) Modified CTAB buffer, initially for leaf samples of Xylopia aromatica and Piper arboreum. Later the procedure with the best results was used to obtain purified RNA from 17 other native species. Based on A260/A280 absorbance ratio the Modified CTAB method was the best for total RNA extraction for those woody species. Ten out of eleven species tested through RT-PCR generated fragments of the expected size from the total RNA extracted by the selected method, confirming it as the best option to obtain high-quality RNA for molecular analyses and for use in the detection of viruses infecting these tree species.

Trizol

Arboreal locomotion

10.1590/2175-7860202071085

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Identification and mapping of resistance genes to Phakopsora pachyrhizi in soybean (Glycine max L.) accession PI 594767-A

Genetics and Molecular Research (2016)

Geisiane Alves Rocha Daniel Pedrosa Alves J. C. Oliveira Sérgio Hermínio Brommonschenkel

The goal of this study was to study resistance inheritance in the soybean (Glycine max L.) accession PI 594767-A to the Phakopsora pachyrhizi isolate PPUFV02, and map the resistance gene(s) identified using microsatellite markers.Crosses between PI 594767-A and the susceptible cultivar 'Conquista' gave rise to the segregating subpopulations 26C-2 and 26C-5, which in the F 2 generation were evaluated for their reactions to PPUFV02.In addition, analyses with microsatellite markers linked to the Rpp1-Rpp5 loci were also performed.The segregation pattern obtained in 26C-2 revealed that resistance was governed by a recessive gene; a 1:2:1 segregation pattern was observed in 26C-5, indicating control by a gene with partial dominance.This variability may have been caused because environmental conditions, particularly temperature, when 26C-5 was assessed were unfavorable for pathogen development, allowing the phenotypic expression of heterozygous alleles in PI 594767-A.A

Phakopsora pachyrhizi

Introgression

10.4238/gmr.15038475

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Groundwater Pollution Risk and Vulnerability Map of the State of São Paulo, Brazil

Water Science & Technology (1991)

Ricardo Hirata C. R. A. Bastos Geisiane Alves Rocha D. C. Gomes Mara Akie Iritani

Aiming to provide a global diagnosis of the actual threat of pollution to the most important aquifers of the State of São Paulo, Brasil, due to its intensive industrialization and agriculture, three government institutions -the Instituto Geologico (Geological Institute), the CETESB (Environmental Sanitary Technology Company) and the DAEE (Water and Eleotrical Energy Department) - carried out a regional survey to define the aquifers' vulnerability to pollution and for recording and ranking the potential contaminant sources. This paper describes the results of this survey, excluding the metropolitan area of the city of São Paulo. More than 500 industries were recorded and ranked according to their low, moderate or high potential contaminant load for impacting groundwater. Thirty - one aquifer units were defined and a 1:500.000 scale vulnerability map was produced. Critical areas were defined and recommended as being priority sites for the adoption of control measures.

Vulnerability

Industrialisation

Groundwater Pollution

10.2166/wst.1991.0348

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Efeito de diferentes tensões de água no solo e do tratamento magnético da água de irrigação no crescimento e na produção da alface

Contribuciones a las Ciencias Sociales (2024)

Cristiany Zuianne do Rosário Nojoza Carvalho Álvaro Henrique Cândido de Souza Cássio Jardim Tavares C Silva Wilson Costa Correia

A otimização do uso da água é essencial para a agricultura sustentável, especialmente diante do aumento da demanda por alimentos e da crescente escassez hídrica. No cultivo de hortaliças como a alface, a busca por aumento da produtividade e da eficiência do uso da água exige ferramentas que possam auxiliar a aplicação de água no momento e quantidade correta. O objetivo deste trabalho foi avaliar a aplicação de água tratada magneticamente na cultura da alface, diferentes tensões de água no solo (-15 kPa e – 30 kPa) e a interação entre esses dois fatores nas variáveis de crescimento e na produção da cultura da alface. Foi verificado que apesar da tensão de água no solo de – 15 kPa demandar maior volume de água ao longo do ciclo, manejar a irrigação considerando essa tensão crítica, proporciona maior número de folhas, massa fresca e seca da parte aérea e eficiência do uso da água. O tratamento magnético da água de irrigação reduziu a massa seca da parte aérea e o volume de água necessário ao longo do ciclo.

10.55905/revconv.17n.13-395

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Plant-associated bacteria mitigate drought stress in soybean

Environmental Science and Pollution Research (2018)

Samuel J. Martins Geisiane Alves Rocha Hyrandir Cabral de Melo Raphaela de Castro Georg Cirano José Ulhôa

Sclerotinia Sclerotiorum

Drought Tolerance

Stomatal Conductance

10.1007/s11356-018-1610-5

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Citations (51)

Seleção de acessos de tomateiro resistentes à murcha-de-verticílio

Tropical Agricultural Research (2017)

Renato Carrer Filho Renata Maria de Oliveira Vanessa Duarte Dias Geisiane Alves Rocha Érico de Campos Dianese

Vascular wilting in tomato plants is an important disease caused by soil-inhabiting pathogens, especially Verticillium dahliae, which results in significant production losses. Control measures against this disease are difficult to implement due to intrinsic pathogen characteristics, such as high adaptability to the subterranean environment, in association with the host, and development of resistance structures that remain viable in the soil for long periods. The introgression of genes that express resistance is the main control measure and requires a continuous characterization program of resistant accessions. This study aimed at identifying tomato (Solanum lycopersicum) accessions resistant to V. dahlia, by using the phenotypic and genotypic methods. The reaction of 33 tomato accessions to different V. dahliae isolates was reinforced by molecular analysis, through markers linked to Ve resistance genes. The combination of bioassays and specific molecular markers showed a high correlation (94.3 %), with the selection of 25 accessions resistant to V. dahliae.

Verticillium dahliae

Verticillium wilt

Wilting

Introgression

Dahlia

Germ plasm

Source

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