C. Eftimiadi

University of Genoa

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G. Rialdi

Institute for Macromolecular Studies

Michela Tonetti

University of Genoa

Oliviero Sacco

Institute of Biosciences and Bioresources

S. Oddera

Ospedale San Carlo

Michela Silvestri

Istituto Giannina Gaslini

Giuseppe Botta

University of Udine

Annalisa Cavallero

Azienda Ospedaliera San Gerardo

Mangiante Pe

University of Genoa

C. De Leo

Gian Carlo Schito

University of Genoa

Cooperative Institutions

University of Genoa

Ospedale Policlinico San Martino

Istituti di Ricovero e Cura a Carattere Scientifico

Czech Academy of Sciences, Institute of Microbiology

Istituto Giannina Gaslini

Boston University

Novant Health Forsyth Medical Center

University of Milan

Harvard University Press

Hospital Infantil de México Federico Gómez

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[Effect of pidotimod on phagocytosis and intracellular killing of Staphylococcus aureus by human circulating polymorphonuclear neutrophils and alveolar macrophages].

PubMed (1993)

S. Oddera Michela Silvestri Oliviero Sacco C. Eftimiadi Rossi Ga

Immunostimulant

Pulmonary alveolus

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Citations (1)

High performance liquid chromatographic strategy suitable for the analysis of exopolysaccharides extracted from pathogenic bacteria

Biomedical Chromatography (1992)

L. Radin Simona Sivori C. Eftimiadi Claudio Lucarelli

Abstract A high performance liquid chromatographic (HPLC) method has been developed to permit the rapid comparison of acidic polysaccharides of diverse compositions and the sensitive determination of their constituents. It is based on two combined analyses of the polysaccharide hydrolysates—a separation of the released compounds by ion‐moderated partition chromatography with UV detection at two wavelengths and a separation of the sugar dansylhydrazine derivatives by reversed phase chromatography. The former permits identification and quantitation of uronic and carboxylic acids, the latter permits more sensitive and specific determination of the neutral aldoses. Some bacterial exopolysaccharides have been used to demonstrate the validity of this HPLC procedure for the chemical characterization of uronic acid‐containing polysaccharides. This method appears to be useful for studying capsular polysaccharides, which are involved in the evasion of phagocytosis by pathogenic bacteria.

Uronic acid

10.1002/bmc.1130060303

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A new calorimetric technique to analyze bacteria-phagocyte interactions.

PubMed (1985)

C. Eftimiadi G. Rialdi

We have assessed the use of a batch calorimeter in the analysis of bacteria phagocyte interactions. Results obtained using different strains of staphylococci agree with our previously reported flow calorimetric data. Phagocytosis of Staphylococcus aureus produces a greater metabolic activation energy expenditure with respect to other "saprophytic" staphylococci. The results obtained indicate that the batch calorimeter better fulfills all the requirements for routine phagocytosis assay.

Phagocyte

Calorimeter (particle physics)

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Divergent effect of the anaerobic bacteria by‐product butyric acid on the immune response: suppression of T‐lymphocyte proliferation and stimulation of interleukin‐1 beta production

Oral Microbiology and Immunology (1991)

C. Eftimiadi Philip Stashenko Maurizio S. Tonetti P Mangiante Rosanna Massara

The effects of short‐chain fatty acids (SCFA) produced by anaerobic bacteria, namely propionic, butyric and iso‐butyric on T‐cell proliferation was investigated. A dose‐dependent inhibition of both phytohemagglutinin‐induced blastogenesis and mixed lymphocyte culture was observed in the millimolar range of SCFA concentrations. The tested SCFA displayed different levels of suppression. The degree of activity was in the following order: butyrate > propionate > iso‐butyrate. T‐cell inhibition was partially reversed, at least for propionic and iso‐butyric acids, by increasing the concentration of macrophages in the assay system. Furthermore, butyric acid displayed an interesting biphasic stimulation of monocyte interleukin‐1 beta production, a cytokine with a powerful bone‐resorbing activity. Since millimolar concentrations of SCFA are present in gingival fluid from periodontal pockets, the observed results support the role of these byproducts of anaerobic metabolism in the pathogenesis of periodontal diseases.

BETA (programming language)

10.1111/j.1399-302x.1991.tb00446.x

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Citations (41)

Increased Energy Expenditure by Granulocytes During Phagocytosis of Staphylococcus aureus Compared with Other Staphylococci

The Journal of Infectious Diseases (1984)

C. Eftimiadi G. Rialdi

The heat produced by human neutrophils during phagocytosis of different strains of Staphylococcus was analyzed by a flow calorimetric technique. At the same bacteria/leukocyte ratio Staphylococcus aureus yielded heat effects about twice that of other saprophytic staphylococci tested. In parallel experiments the degree of phagocytosis of radiolabeled bacteria was evaluated: S. aureus strains were the least phagocytosed. These data indicate that, for a single phagocytosed bacterium, S. aureus strains yield heat effects three to four times greater than do the saprophytic staphylococci tested (P less than .001). Previous studies have shown that the heat produced by activated neutrophils derives essentially from the aerobic catabolism of glucose involved in the production of bactericidal O2 metabolites and is directly proportional to O2 consumption. The results reported here indicate that less efficient internalization of a staphylococcal strain is correlated with a greater energy expenditure for O2 metabolite formation during the metabolic burst.

Catabolism

Internalization

10.1093/infdis/150.3.366

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Citations (13)

Effeto di pidotimod sulla fagocitosi e sul killing intracellulare di staphylococcus aureus da parte di polimorfonucleati neutroli circolanti e di macrofagi alveolari umani

Drugs under experimental and clinical research (1993)

S. Oddera Michela Silvestri Oliviero Sacco C. Eftimiadi Giovanni Rossi

Meticillin

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Estimation of serum antibody to subgingival species using checkerboard immunoblotting

Oral Microbiology and Immunology (1997)

Dimitra Sakellari S. S. Socransky Serge Dibart C. Eftimiadi Martin A. Taubman

Measurement of serum antibody to subgingival bacterial species has been useful in discriminating possible periodontal pathogens and in assessing the host's immune response to subgingival species. An immunoassay system was developed to measure the level of serum antibody to multiple subgingival species in multiple serum samples on a single nitrocellulose membrane. The principle steps of the assay are the following: 1) binding of antigens from bacterial preparations and protein A in parallel lanes on nitrocellulose membranes; 2) incubation of known concentrations of human immunoglobulin as well as various dilutions of serum from patients in lanes perpendicular to the antigen lanes; 3) incubation of the membrane with a peroxidase‐conjugated second antibody; 4) detection of positive reactions by enhanced chemilurninescence. Emitted light was captured on a photographic film in which the positive reactions appeared as squares at the intersections of antigens with appropriate antibody. Antibody was quantified using a laser densitometer to compare the signal intensity of unknown samples with the ones generated by known amounts of human immunoglobulin captured on the same membrane. The assay permitted simultaneous screening and/or quantification of antibody in as many as 45 serum samples against up to 45 bacterial species. The mean and standard error of the coefficients of variation for replicates within an assay averaged 7.3±2.3%. Coefficients of variation of the assay run on different days for serum antibody to a range of subgingival species averaged 10.1 ±2.1%. Checkerboard immunoblotting is a simple and rapid immunoassay to permit quantification and/or screening of antibody to multiple subgingival species or antigens in multiple serum samples.

Serial dilution

10.1111/j.1399-302x.1997.tb00395.x

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Citations (61)

In vitro studies on the interactions of bacteria with prosthetic devices.

Journal of Chemotherapy (1989)

Botta Ga C. Eftimiadi Annalisa Cavallero Alessandra Arzese O. Soro

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Enhancement of mutagenesis in gamma ray exposed Escherichia coli by plasmids of different incompatibility groups.

PubMed (1985)

C. Eftimiadi C. De Leo Eugenio A. Debbia

We have analyzed the correlation between plasmid incompatibility group and enhanced mutagenesis in gamma ray exposed Escherichia coli WP2. The data reported indicate that plasmids of different incompatibility groups are involved in DNA repair mechanisms and suggest that the damage of the DNA produced by gamma rays or other physical agents and by chemical mutagens stimulates a common mechanism of bacterial DNA repair.

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Prevalence of Streptococcus mutans and dental decay in schoolchildren living in Genoa (Italy)

European Journal of Epidemiology (1990)

C Leo Rosa Coppola Gonzalo Blasi C. Eftimiadi M. Salvarani

Dental decay

10.1007/bf00145790

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Citations (12)