ЕН

Е. Н. Филатова

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

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ОВ

О. В. Уткин

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

НА

Н. А. Сахарнов

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

M. I. Popkova

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

ЕА

Е. А. Кулова

Privolzhsky Research Medical University

ОВ

О. В. Уткин

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

N. B. Presnyakova

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

E. V. Anisenkova

Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Novikov Vv

N. I. Lobachevsky State University of Nizhny Novgorod

Eugene Genikhovich

Voeikov Main Geophysical Observatory

D. I. Knyazev

Privolzhsky Research Medical University

Cooperative Institutions

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Privolzhsky Research Medical University

Ministry of Health of the Russian Federation

N. I. Lobachevsky State University of Nizhny Novgorod

Ural research Institute of Phthisiopulmonology

Institute of Molecular Biology and Biotechnology

Sechenov University

Siberian State Medical University

Voeikov Main Geophysical Observatory

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SIRNA-ОПОСРЕДОВАННЫЙ НОКДАУН ГЕНОВ В ПЕРВИЧНЫХ КЛЕТКАХ МЛЕКОПИТАЮЩИХ И ЧЕЛОВЕКА, "Успехи современной биологии"

Успехи современной биологии (2018)

Е. Н. Филатова Н. Б. Преснякова О. В. Уткин

Малые интерферирующие РНК (siRNA) применяют для специфического подавления экспрессии генов в клетках млекопитающих и человека различного происхождения. Перспективным является использование siRNA для терапии некоторых инфекционных заболеваний. На сегодня основным препятствием для применения генного нокдауна в медицинской практике является недостаточная эффективность и безопасность метода в отношении первичных клеток млекопитающих и человека. Изложены современные представления о методах трансфекции siRNA в отношении первичных клеток млекопитающих, об их преимуществах и ограничениях. Приведены результаты собственных исследований возможности липофекции siRNA в первичные иммунокомпетентные клетки крови человека. Описаны основные принципы подбора нуклеотидных последовательностей siRNA, обеспечивающие повышение эффективности и снижение выраженности побочных эффектов генного нокдауна.

10.7868/s0042132418060030

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Полноразмерная и альтернативная формы мРНК гена CD38 в клетках перевиваемых линий, полученных от больных раком Толстого кишечника

Российский биотерапевтический журнал (2012)

Е. Н. Филатова А. Д. Перенков Д. В. Новиков А. Ю. Барышников Novikov Vv

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Drug resistance of Mycobacterium tuberculosis in the Ural region of Russian Federation

Tuberculosis (2019)

Е. Н. Филатова Vadim Testov Konstantin Glebov Igor Medvinsky Anna Panova

10.1183/13993003.congress-2019.pa2959

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A Virtual Instrument for Spectral Entropy Estimation of Heart Rate

Izvestiya of Altai State University (2016)

S. Ostanin Е. Н. Филатова

10.14258/izvasu(2016)1-07

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DEVELOPMENT AND VALIDATION FOR INTRA-SPECIES CLASSIFICATION OF HHV6A AND HHV6B MOLECULAR GENETIC DIVERSITY

Russian Journal of Infection and Immunity (2024)

Е. Н. Филатова О. В. Уткин A. Е. Khrulev N.N. Zaitsevа

Human herpesvirus 6A (HHV6A) and human herpesvirus 6B (HHV6) are ubiquitous viruses that infect more than 95% of the population. Clinical manifestations of HHV6 infection and associated diseases are diverse, which may depend on virus molecular genetic characteristics (genovariants). Estimating the significance of the molecular genetic diversity is complicated due to the lack of proper classification. The aim of the study was to develop an intraspecies HHV6A and HHV6B classification. Using 50 and 207 HHV6A and HHV6B full-genome sequences retrieved from the NCBI Nucleotide database, various fragments of virus genome were analyzed. Multiple sequence alignment was performed using MAFFT L-INS-i algorithm; F81 nucleotide substitution model and maximum likelihood method were used to construct dendrograms. Nucleotide substitutions were determined relative to reference sequences X83413 (HHV6A) and AF157706 (HHV6B). Genovariants were defined based on the nucleotide substitutions in variable positions of the genomic fragment. The results were confirmed by constructing dendrograms. An opportunity of using fragments of HHV6A and HHV6B genomes to construct an intraspecies classification was assessed. Fragments U90 (part206) and U90B(part431) were selected as optimal. Based on the nucleotide sequences of the fragments, the intraspecies classification for HHV6A and HHV6B was constructed, including seven genovariants of each virus. The genovariants were characterized by unique nucleotide composition in the signature positions. A minimum (0.001 or less for both viruses) nucleotide diversity within the isolated genovariants was established. The classification reflects the phylogenetic relationships of circulating and inherited chromosomally integrated forms of HHV6A and HHV6B: divergence of HHV6A genovariants depending on its persistence form and integration site and coevolution of two HHV6B forms within several genovariants. Further studies on virus molecular genetic diversity in different regions of Russia and abroad may supplement the classification. The method of HHV6A and HHV6B classification is characterized by simplicity, technological accessibility and can be implemented in laboratories of different levels of technical equipment. The classification can be used to analyze an effect of virus molecular genetic diversity on the clinical characteristics of associated diseases, optimize the epidemiological surveillance system and develop new approaches for diagnostics, prevention, and treatment of HHV6 infection.

10.15789/2220-7619-dav-17810

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ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS

Russian Journal of Infection and Immunity (2016)

Е. Н. Филатова E. V. Anisenkova N. B. Presnyakova T. D. Sycheva Е. А. Кулова

Acute infectious mononucleosis is a widespread viral disease, which most often manifests in childhood. The development of acute infectious mononucleosis is accompanied by the change of the CD4+/CD8+ T-lymphocytes ratio and the increase of the virus-specific CD8+ cytotoxic T-lymphocytes number. One of the T-lymphocytes number regulation mechanisms is the modulation of their progenitor cells apoptosis. The death receptor CD95 takes part in the regulation of T-lymphocytes apoptosis, including naïve T-cells. We studied the effect of CD95 receptor activation on apoptosis of naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes in healthy children and children with acute infectious mononucleosis. In this study children with acute infectious mononucleosis at the age of 9 to 16 years were included. For comparison healthy children of the same age with no clinical and laboratory signs of the disease were used. Naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes were isolated by negative magnetic immunoseparation. The analysis of naïve T-cells apoptosis and the CD95 receptor surface expression density was performed by using the flow cytometry analysis. The analysis of T-cells was performed in three variants: freshly isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes, and also cells after 24 hours of the cultivation with anti-CD95 monoclonal antibodies or without them. In healthy children both CD95– and CD95+ naïve CD4+ T-lymphocytes underwent apoptosis. In children with acute infectious mononucleosis CD95– naïve CD4+ T-lymphocytes lost their susceptibility to apoptosis induction. In healthy children and children with acute infectious mononucleosis CD95– naïve cytotoxic CD8+ T-lymphocytes were resistant to apoptosis in contrast to CD95+ naïve CD4+ T-lymphocytes. In healthy children CD95 receptor did not induce apoptosis of isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes. In children with acute infectious mononucleosis CD95 receptor was involved in inhibition of apoptosis of naïve cytotoxic CD8+ T-lymphocytes and did not effect on the level of apoptosis of naïve CD4+ T-lymphocytes. We suggest that CD95-dependent suppression of naïve cytotoxic CD8+ T-lymphocytes apoptosis is a protective mechanism for the maintenance of a sufficient number of cytotoxic T-lymphocytes in the blood for the realization of effective antiviral immune response.

Mononucleosis

Fas ligand

10.15789/2220-7619-2016-3-207-218

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FIRST DATA ON THE MOLECULAR GENETIC DIVERSITY OF HHV6B AND ITS IMPACT ON THE CLINICAL AND LABORATORY COURSE OF MONONUCLEOSIS-LIKE SYNDROME IN CHILDREN

Russian Journal of Infection and Immunity (2025)

Е. Н. Филатова Н. А. Сахарнов Nikita A. Suslov M. I. Popkova О. В. Уткин

Human herpes virus 6B (HHV6B) is a ubiquitous causative agent of mononucleosis-like syndrome (MLS) and other diseases. The diversity of HHV6B infection may be due to virus genetic polymorphism. Molecular genetic diversity of circulating HHV6B, as well as its impact on the clinical and laboratory features of HHV6B infection have not been studied. The aim of the work was to study the intraspecific diversity of HHV6B circulating among children in Nizhny Novgorod (Russia) region, and to investigate its effect on MLS clinical and laboratory features. Blood leukocytes and saliva DNA samples from healthy children and children with HHV6B infection were analyzed for HHV6B DNA detection. Original intraspecific HHV6B classification based on detecting signature nucleotide substitutions in the sequence of the U90 gene fragment, was used for virus typing. A relationship between HHV6B genovariant and clinical/laboratory MLS characteristics was studied using multivariate statistical methods. Circulation of four HHV6B genovariants was shown among children in Nizhny Novgorod region: HHV6B/GV2e, HHV6B/GV2b (dominant genovariants), HHV6B/GV1a and HHV6B/GV2a (minor genovariants). Children with MLS infected with HHV6B/GV2e genovariant were more likely to have laboratory signs of cytolysis and increased peripheral blood lymphocyte count. Children with MLS infected with the HHV6B/GV2b genovariant were more likely to have higher mature neutrophil level. An increase of HHV6B viral load in leukocytes was accompanied by aggravated intoxication symptoms. Co-infection with the Epstein-Barr virus (EBV) led to more severe symptoms of acute tonsillitis, hepatosplenomegaly, and lymphadenopathy. Patients with EBV were more likely to have higher ALAT and lymphocyte level along with decreased mature neutrophil count. In children with MLS, EBV coinfection caused smaller HHV6B viral load in blood leukocytes (in children with HHV6B DNA 10 or more copies/105 cells) and lower HHV6B DNA detection frequency in saliva (in children with HHV6B DNA less than 10 copies/105 leukocytes). The results show the influence of genetic HHV6B polymorphism upon the clinical and laboratory features of MLS in children, and also demonstrate that HHV6B/GV2e and EBV cause similar disease symptoms.

Mononucleosis

Course (navigation)

10.15789/2220-7619-fdo-17827

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The Role of Noncoding mRNA Isoforms in the Regulation of Gene Expression

Russian Journal of Genetics (2018)

Е. Н. Филатова О. В. Уткин

Human genetics

10.1134/s1022795418080057

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Citations (2)

ОСОБЕННОСТИ РЕГУЛЯЦИИ АПОПТОЗА КЛЕТОК, ИНФИЦИРОВАННЫХ ЦИТОМЕГАЛОВИРУСОМ И ВИРУСОМ ЭПШТЕЙНА-БАРР, "Успехи современной биологии"

Успехи современной биологии (2017)

Н. А. Сахарнов О. В. Уткин Д.И. Князев Е. Н. Филатова В. Д. Цветкова

10.7868/s0042132417040068

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A method for mapping the air pollution in cities with the combined use of measured and calculated concentrations

International Journal of Environment and Pollution (2002)

Eugene Genikhovich Е. Н. Филатова Alexander Ziv

The aim of this paper is to develop the technique for mapping the air pollution in cities using measured and computed concentrations. It is based on the combined use of deterministic and stochastic modelling. Two different approaches are applied to extrapolate the measurements to the regular grid points covering the city. The first uses the method of the principal components, and the second is based on optimal interpolation or "kriging" of meteorological fields. The results of the application of this model for the mapping of air pollution in the city of Pskov, Russia, are given.

Interpolation

10.1504/ijep.2002.000694

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Citations (17)