Yankai Liu

Zhengzhou University

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Ying-Han Chen

New York University

Jingming Zhou

Zhengzhou University

Aiping Wang

Zhengzhou University

Hongliang Liu

Zhengzhou University

Gaiping Zhang

Henan Agricultural University

Paolo Melchiorre

University of Bologna

Yanhua Qi

Zhengzhou University

Xue-Jiao Lv

Yumei Chen

Zhengzhou University

Dehai Li

Ocean University of China

Cooperative Institutions

Pontifical Catholic University of Rio de Janeiro

370

Universidade Estadual de Londrina

365

A. Alikhanyan National Laboratory

363

Beijing Normal University

324

Chinese Academy of Sciences

320

Université Libre de Bruxelles

317

Institute of High Energy Physics

312

Federico Santa María Technical University

237

China Academy of Space Technology

199

China Institute of Atomic Energy

198

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A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein

International Journal of Molecular Sciences (2022)

Zehui Li Aiping Wang Jingming Zhou Yumei Chen Hongliang Liu

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the pathogenic agent leading to COVID-19. Due to high speed of transmission and mutation rates, universal diagnosis and appropriate prevention are still urgently needed. The nucleocapsid protein of SARS-CoV-2 is considered more conserved than spike proteins and is abundant during the virus' life cycle, making it suitable for diagnostic applications. Here, we designed and developed a fluorescent immunochromatography assay (FICA) for the rapid detection of SARS-CoV-2-specific antibodies using ZnCdSe/ZnS QDs-conjugated nucleocapsid (N) proteins as probes. The nucleocapsid protein was expressed in E.coli and purified via Ni-NTA affinity chromatography with considerable concentration (0.762 mg/mL) and a purity of more than 90%, which could bind to specific antibodies and the complex could be captured by Staphylococcal protein A (SPA) with fluorescence displayed. After the optimization of coupling and detecting conditions, the limit of detection was determined to be 1:1.024 × 105 with an IgG concentration of 48.84 ng/mL with good specificity shown to antibodies against other zoonotic coronaviruses and respiratory infection-related viruses (n = 5). The universal fluorescent immunochromatography assay simplified operation processes in one step, which could be used for the point of care detection of SARS-CoV-2-specific antibodies. Moreover, it was also considered as an efficient tool for the serological screening of potential susceptible animals and for monitoring the expansion of virus host ranges.

Coronavirus

10.3390/ijms23116225

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Citations (10)

Effect of intestinal lymphatic pathway on free radical and inflammatory mediator of myocardium in shock rats

Zi‐Gang Zhao Chun‐Yu Niu Run-hua Chen Yuping Zhang Jing Zhang

Aim To observe the effect of mesenteric lymph duct ligation on free radical and inflammatory mediator of myocardium with severe hemorrhagic shock in rats at different period, and explore the effect of intestinal lymphatic pathway on myocardium injury pathogenesis in shock rats. Methods 78 male Wistar rats were divided into the sham group, shock group and ligation group. The model of serious hemorrhagic shock was established in shock group, ligation group, and mesenteric lymph was blocked by ligating mesenteric lymph duct in ligation group after resuscitate. All rats were executed and taken out heart making for homogenate of 10 percent to determine the MDA, SOD, tumor necrosis factor-alpha (TNFalpha), interleukin-6 (IL-6), myeloperoxidase (MPO), NO and NOS at after shock 90 min, after transfusion and resuscitate 0 h, 1 h, 3 h, 6 h, 12 h and 24 h etc. different times, and the expression of inducible nitric oxide synthase (iNOS) mRNA in myocardium was detected by RT-PCR. Results The contents of MDA, TNFalpha, IL-6, MPO, NO, NOS and iNOS expression in myocardium of shock group were rising after transfusion and resuscitate, and that was higher level at 3 h to 12 h, and that was significantly higher than sham group, the activity of SOD was significantly lower than sham group. The contents of MDA, TNFalpha, IL-6, MPO, NO, NOS and iNOS expression in myocardium of ligation group were significantly lower than that of shock group at sameness points, and the SOD activity was higher. Conclusion The mesenteric lymph duct ligation and blocking mesenteric lymph could reduce the PMN detaining, decrease the discharging of TNFa and IL-6, reduce the NO and expression of iNOS mRNA, and reduce the releasing of free radical and consuming of SOD.

Pathogenesis

Mesenteric lymph nodes

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Citations (1)

CCDC 1911881: Experimental Crystal Structure Determination

The Cambridge Structural Database (2019)

Chen Wang Ying-Han Chen Hui-Chun Wu Cong Wang Yankai Liu

10.5517/ccdc.csd.cc225glb

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CCDC 1877711: Experimental Crystal Structure Determination

The Cambridge Structural Database (2019)

Chaochao Xie Rui Tan Yankai Liu

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccdc.csd.cc210xbb

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Asymmetric Organocatalytic One-Pot, Two-Step Sequential Process to Synthesize Chiral Acetal-Containing Polycyclic Derivatives from Cyclic Hemiacetals and Enones

The Journal of Organic Chemistry (2017)

Chao Liu Yankai Liu

We have developed an efficient one-pot, two-step sequential process to synthesize biologically and synthetically important chiral acetal-containing polycyclic derivatives. This novel protocol had been proved to proceed via Michael-lactolization-oxocarbenium ion ring-closing sequence, which was initiated by a key reactive enamine intermediate and interrupted the previously established reaction pathway of two different enones used in this work, and generated the corresponding cycloadducts with excellent stereoselectivity bearing up to seven continuous stereocenters. Both chiral and racemic starting cyclic hemiacetals worked well in this strategy. The synthetic applications of the obtained polycyclic products have also been demonstrated.

Stereocenter

Enamine

Oxocarbenium

10.1021/acs.joc.7b01915

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Citations (21)

Grouting rock fractures under condition of flowing water

Carbonates and Evaporites (2020)

Yanxu Guo Qingsong Zhang Fei Xiao Rentai Liu Zhenjun Wang

10.1007/s13146-020-00619-z

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Citations (20)