Objectives: Whether a relationship between fish consumption and non-Hodgkin lymphoma (NHL) risk exists is an open issue. We carried out a meta-analysis to explore this association according to the published observational studies. Methods: We performed a search of databases in MEDLINE and EMBASE to identify relevant studies. We derived meta-analytic estimates using random-effects models, and assessed between-study heterogeneity using the Cochran's Q and I2 statistics. Results: We identified a total of seven case–control and two prospective cohort studies, including 7696 subjects with NHL. The summary relative risks (SRRs) estimated for NHL were 0.80 (95% confidence intervals (CIs): 0.68–0.94) for those in the highest fish consumption category compared with those in the lowest consumption category. There was evidence of significant heterogeneity across studies (Q = 26.72, Pheterogeneity = 0.002, I2 = 66.3%). Stratified analysis by study design indicated that a significant risk association between fish consumption and NHL was observed in case–control studies, but not in cohort studies. Based on the dose–response meta-analysis, the SRRs of NHL were 0.85 (95% CIs: 0.71–1.01) for three servings increased per week of fish consumed with evidence of significant heterogeneity (Pheterogeneity = 0.007, I2 = 63.9%). Conclusions: Findings from our meta-analysis indicate that consumption of fish may be not related to NHL risk.
Abstract Objective To study Ermiaosan in the treatment of UC by using network pharmacology and molecular docking, and to provide references for experiments and clinical application for treating UC with dampness-heat syndrome. Methods The main active chemical components of Ermiaosan were screened out through TCMSP, the targets of components were obtained from TCMSP, the SwissTargetPrediction, TTD and the DrugBank database, and these targets genes were retrieved by UniProt database, the disease genes were obtained from TTD and Genecard database. String tool was used to constructed the PPI network, to built these components and their corresponding targets, Cytoscape software was applied to merge the networks and screen out the core network. And Bioinformatic analysis was performed using the OECloud tools to explore the enrichment analyses of GO and KEGG. Molecular docking was applied to check the affinity between the components and selected targets. Results Forty-six main active components were predicted from Ermiaosan, and 408 intersection genes were screened from drug-disease genes. The enrichment included PI3K–Akt, TNF and HIF-1 signaling pathway, and the networks analysis showed that Ermiaosan acted on seven key targets AKT1, TNF, IL6,TP53, VEGFA, IL1B and CTNNB1 to play roles in treating UC. Molecular docking showed that top 3 chemical components could bind stably with these targets. Conclusion Ermiaosan can relieve dampness-heat syndrome of UC, the possible potential mechanism might be related to the targets AKT1, TNF, IL6,TP53, VEGFA, IL1B and CTNNB1 linked with TNF, PI3K-Akt, and HIF-1 signaling pathway, it will provide meaningful references for further study in experiments and clinical investigations.
Abstract Background Dysregulation of iron metabolism has been shown to have significant implications for cancer development. We aimed to investigate the prognostic and immunological significance of iron metabolism-related genes (IMRGs) in nasopharyngeal carcinoma (NPC). Methods Multiple Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) datasets were analyzed to identify key IMRGs associated with prognosis. Additionally, the immunological significance of IMRGs was explored. Results A novel risk model was established using the LASSO regression algorithm, incorporating three genes (TFRC, SLC39A14, and ATP6V0D1).This model categorized patients into low and high-risk groups, and Kaplan–Meier analysis revealed significantly shorter progression-free survival for the high-risk group ( P < 0.0001). The prognostic model’s accuracy was additionally confirmed by employing time-dependent Receiver Operating Characteristic (ROC) curves and conducting Decision Curve Analysis (DCA). High-risk patients were found to correlate with advanced clinical stages, specific tumor microenvironment subtypes, and distinct morphologies. ESTIMATE analysis demonstrated a significant inverse relationship between increased immune, stromal, and ESTIMATE scores and lowered risk score. Immune analysis indicated a negative correlation between high-risk score and the abundance of most tumor-infiltrating immune cells, including dendritic cells, CD8 + T cells, CD4 + T cells, and B cells. This correlation extended to immune checkpoint genes such as PDCD1, CTLA4, TIGIT, LAG3, and BTLA. The protein expression patterns of selected genes in clinical NPC samples were validated through immunohistochemistry. Conclusion This study presents a prognostic model utilizing IMRGs in NPC, which could assist in assessing patient prognosis and provide insights into new therapeutic targets for NPC.
Mycobacterium intracellulare-caused pulmonary infections have mostly been reported in immunocompromised hosts, while cutaneous M. intracellulare infections are rare. We describe here an immunocompetent patient with cutaneous lesions due to M. intracellulare, which was diagnosed by acid-fast staining, in vitro culture, histopathology, and PCR-restriction fragment length polymorphism analysis and gene sequencing of heat-shock protein (hsp) 65 and 16S rDNA genes. In vitro susceptibility testing was also carried out and the patient was successfully treated with clarithromycin, rifampicin, and ethambutol.
Objective
To investigate chromosomal aneuploidy of chorionic villior tissue from embryo arrestor spontaneous miscarriage by the method of multiplex ligation-dependent probe amplification(MLPA), and to provide genetic etiology data for recurrent spontaneous abortion(RSA) and embryo arrest.
Methods
The clinical retrospective analysis was used during November 2013 to October 2016 in Shenzhen Maternity and Child Healthcare Hospital. The genomic DNA of 7 036 chorionic villi or tissue samples from embryo arrestor pregnancy loss were extracted by resin and Proteinase K, following detected by multiplex ligation-dependent probe amplification, and then analyzed by descriptive and frequency statistic using SPSS17.0 software.
Results
Totally 2 984 samples (42.41%) with chromosomal aneuploidy were detected, of which, the top five were trisomy 16(826)11.74%, monosomy X (Turner, 401)5.70%, trisomy 22(247)3.51%, trisomy 13(149)2.12% and trisomy 21(144)2.05%. Aneuploidy 19 or 17 were rare, just 1 and 3 cases respectively. The aneuploidy of chromosome 1 was not discovered yet. There were 161 samples (2.28%) for segmental deletion and/or duplication cases. Besides of the monosomy X(Turner, 401), monsomy 21 was also high frequency(17, 0.24%). In addition, 56 samples(0.80%)were double trisomic, while 2 samples(0.03%)were tris-trisomy. Thirteen samples(0.18%)were heterosome trisomy, and five samples(0.07%)were Turner combined trisomy. Fifteen samples(0.21%) were unbalanced translocation of the arm, while 20 samples(0.28%) were nonhomologous chromosome translocation.
Conclusions
Trisome and monosome were the majority of chromosome abnormality (above 90%), as well as the main cause of spontaneous miscarriage. Meanwhile the chromosome aneuploidy (including segment deletion or duplication) could be screened by MLPA quickly, thoroughly and efficiently, further more discover the carrier of balanced translocation in couples and provide valuable genetic information for next pregnancy.(Chin J Lab Med, 2017, 40: 598-601)
Key words:
Abortion, spontaneous; Chorionic villi; Chromosome aberrations; Aneuploidy; Multiplex polymerase chain reaction
Chromosome 15q24 microdeletion syndrome is a rare disease. To date, only 40 cases have been reported. Here, we also confirmed a 15q24 microdeletion syndrome in a chorionic villus of miscarriage.The microdeletion was screened by multiplex ligation-dependent probe amplification (MLPA) and then identified by chromosomal microarray analysis (CMA).A 15q24 microdeletion syndrome was screened by MLPA in the chorionic villus of miscarriage in a Chinese family and was confirmed to be a de novo 3.143 Mb 15q24.1q24.2 deletion (chr15:72930195-76073450) by chromosomal microarray analysis (CMA).We first reported the 15q24 microdeletion syndrome screened by MLPA in Chinese population, and we also considered that the technique of MLPA with a suitable kit and probe could screen such a rare microdeletion quickly, economically, and efficiently.
Objective
To analyze the frequency and type of mutations in the c-kit gene, and to measure the expression of c-kit protein in patients with acral malignant melanoma.
Methods
Skin tissue specimens were collected from the lesions of 115 patients with cutaneous malignant melanoma（CMM）and 30 patients with acral melanocytic nevi, as well as normal skin of 15 healthy human controls. PCR and DNA sequencing were performed to analyze the sequence of the c-kit gene, and immunohistochemical staining was conducted to observe c-kit protein expression in tissue samples. Statistical analysis was done by Chi-square test, Mann-Whitney U test and Spearman's rank correlation test.
Results
C-kit protein was expressed in 90.3%（84/93）of acral CMM specimens, 81.8%（18/22）of non-acral CMM specimens, and 63.3%（19/30）of acral melanocytic nevus specimens. Among 58 acral invasive CMM specimens, 29 showed strong expression of c-kit protein in tumor cells at the dermal-epidermal junction, 8 showed negative or weak c-kit expression in invasive and infiltrating tumor cells in the dermis. The expression rate of c-kit protein was significantly higher in acral CMM specimens than in acral melanocytic nevus specimens（χ2 = 12.14, P 0.05）. C-kit gene mutations were detected in 4 out of the 115 CMM cases, including L576P mutation in 3 cases and K642E mutation in 1 case. All the 4 patients carrying mutations were diagnosed as acral CMM, and exhibited diffuse and strong expression of c-kit protein.
Conclusions
The expression of c-kit protein is stronger in acral than in non-acral invasive CMM lesions. All the mutations detected in these cases are common types of c-kit mutations in acral CMM, but the mutation frequency is lower than that reported in foreign literature.
Key words:
Melanoma; Nevus, pigmented; Proto-oncogene proteins c-kit; Point mutation; C-kit gene
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