Małgorzata Ochota

Wroclaw University of Environmental and Life Sciences

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Wojciech Niżański

Wroclaw University of Environmental and Life Sciences

Izabela Szczerbal

University of Life Sciences in Poznań

Joanna Nowacka‐Woszuk

University of Life Sciences in Poznań

J. Twardoń

Wroclaw University of Environmental and Life Sciences

Michał Dzięcioł

Wroclaw University of Environmental and Life Sciences

MŚ

M. Świtoński

University of Life Sciences in Poznań

Ewa Stańczyk

University of Amsterdam

Agnieszka Antończyk

Wroclaw University of Environmental and Life Sciences

Roland Kozdrowski

Nicolaus Copernicus University

S. Dzimira

Wroclaw University of Environmental and Life Sciences

Cooperative Institutions

Wroclaw University of Environmental and Life Sciences

Instytut Środowiska Rolniczego i Leśnego Polskiej Akademii Nauk

University of Wrocław

Polish Academy of Sciences

École Nationale Vétérinaire de Toulouse

University of Agriculture in Krakow

University of Guelph

Centre National de la Recherche Scientifique

University of Life Sciences in Lublin

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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Total Cell Number and its Allocation to Trophectoderm and Inner Cell Mass in In Vitro Obtained Cats' Blastocysts

Reproduction in Domestic Animals (2016)

Małgorzata Ochota B Wojtasik Wojciech Niżański

The aim of this study was to evaluate the developmental kinetics of cats' blastocysts in connection with their morphology and blastomeres allocation to trophoblast or embryoblast cells. We examined gross blastocyst morphology and the total number of blastomeres together with its allocation to inner cell mass (ICM) or trophectoderm (TE) cells in pre-implantation feline embryos obtained from 6th to 9th day of in vitro culture. From all the investigated embryos, 61.8% developed to early blastocyst, 37.4% to full and 7.6% to hatching blastocyst stage. The total cell number (TCN) varied form 58 cells in early day 6 to 245 in hatching day 8 blastocyst, with the mean 84.9 cells in early, 156.7 in full, and 204.4 in hatching ones. Day 8 blastocyst had the highest number of total cells, together with the highest mean number of ICM regardless of its morphological assessment. Early blastocyst (apart from day 6) had the highest number of arrested cells, while dead cells were the highest in full day 9 blastocyst. More data about the relationship between blastocyst development and morphology would facilitate the selection of optimal blastocysts for further procedures.

Inner cell mass

Blastomere

Trophoblast

10.1111/rda.12684

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A Lack of Association between Polymorphisms of Three Positional Candidate Genes (CLASP2, UBP1, and FBXL2) and Canine Disorder of Sexual Development (78,XX; SRY-Negative)

Sexual Development (2014)

Sylwia Salamon Joanna Nowacka‐Woszuk Izabela Szczerbal S. Dzimira Wojciech Niżański

A disorder of sexual development (DSD) of dogs with a female karyotype, missing SRY gene, and presence of testicles or ovotestes is quite commonly diagnosed. It is suggested that this disorder is caused by an autosomal recessive mutation; however, other models of inheritance have not been definitely ruled out. In an earlier study it was hypothesized that the mutation may reside in a pericentromeric region of canine chromosome 23 (CFA23). Three positional candidate genes (CLASP2, UBP1, and FBXL2) were selected in silico in the search for polymorphisms in 7 testicular or ovotesticular XX DSD dogs, 8 XX DSD dogs of unknown cause (SRY-negative, with enlarged clitoris and unknown histology of gonads), and 29 normal female dogs as a control group. Among the 15 molecularly studied dogs with enlarged clitoris there were 3 new cases of testicular or ovotesticular XX DSD and 4 new cases of XX DSD with unknown cause (histology of the gonads unknown). Altogether, 11 (including 10 novel) polymorphisms in 5′- and 3′-flanking regions of the studied genes were found. The distribution analysis of these polymorphisms showed no association with the DSD phenotypes. Thus, it was concluded that the presence of the causative mutation for testicular or ovotesticular XX DSD in the pericentromeric region of CFA23 is unlikely.

Testis determining factor

Clitoris

Candidate gene

10.1159/000363531

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Przezwyciężanie wyzwań w neonatologii psów i kotów Część II: Postępowanie diagnostyczne i terapeutyczne w przypadku chorób u noworodków

Weterynaria w Praktyce (2016)

W. Bielas Małgorzata Ochota Wojciech Niżański

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Superoxide dismutase and taurine supplementation improves in vitro blastocyst yield from poor-quality feline oocytes

Theriogenology (2015)

Małgorzata Ochota Anna Pasieka Wojciech Niżański

In vitro maturation

10.1016/j.theriogenology.2015.10.042

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Citations (20)

Ultrastructural changes in feline oocytes during ovary storage for 24- and 48-hours

Theriogenology (2022)

Małgorzata Ochota Michał Kulus Wiesława Młodawska Marta Kardasz-Kamocka Katarzyna Haczkiewicz-Leśniak

Organelle

Lipid droplet

10.1016/j.theriogenology.2022.11.025

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A Case of Leucocyte Chimerism (78,XX/78,XY) in a Dog with a Disorder of Sexual Development

Reproduction in Domestic Animals (2014)

Izabela Szczerbal Joanna Nowacka‐Woszuk Wojciech Niżański Sylwia Salamon Małgorzata Ochota

Contents A 1‐year‐old Shih Tzu dog was presented for examination because of abnormal external genitalia. A residual penis with a prepuce was located in a position typical of a male. The dog had no palpable testicles or scrotum. The ultrasound examination revealed the presence of the prostate, but the gonads remained undetectable. Cytogenetic analysis performed on chromosome preparations obtained from lymphocyte culture showed two cell lines – 78, XX and 78, XY . Molecular analysis of 14 polymorphic microsatellite markers allowed us to distinguish leucocyte chimerism from whole body chimerism. The presence of 3 or 4 alleles was confirmed in DNA isolated from blood, while in DNA isolated from hair follicles only 1 or 2 alleles were detected. The case was classified as leucocyte 78, XX /78, XY chimerism. Our study showed that XX / XY leucocyte chimerism might be associated with disorder of sexual development in dogs. Furthermore, it is emphasized that the use of cytogenetic study, in combination with analysis of polymorphic markers in DNA isolated from different somatic cells, facilitates distinguishing between leucocyte and whole body chimerism.

10.1111/rda.12318

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Influence of bitches sex pheromones on the heart rate and other chosen parameters of blood flow in stud dogs (Canis familiaris)

Research in Veterinary Science (2012)

Michał Dzięcioł Ewa Stańczyk Agnieszka Noszczyk-Nowak Wojciech Niżański Małgorzata Ochota

Sexual arousal

Intensity

10.1016/j.rvsc.2012.02.009

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Dead or Alive? A Review of Perinatal Factors That Determine Canine Neonatal Viability

Animals (2022)

Oliwia Uchańska Małgorzata Ochota Maria Eberhardt Wojciech Niżański

The perinatal period has a critical impact on viability of the newborns. The variety of factors that can potentially affect the health of a litter during pregnancy, birth, and the first weeks of life requires proper attention from both the breeder and the veterinarian. The health status of puppies can be influenced by various maternal factors, including breed characteristics, anatomy, quality of nutrition, delivery assistance, neonatal care, and environmental or infectious agents encountered during pregnancy. Regular examinations and pregnancy monitoring are key tools for early detection of signals that can indicate disorders even before clinical signs occur. Early detection significantly increases the chances of puppies’ survival and proper development. The purpose of the review was to summarize and discuss the complex interactions between all elements that, throughout pregnancy and the first days of life, have a tangible impact on the subsequent fate of the offspring. Many of these components continue to pose challenges in veterinary neonatology; thus, publications presenting the current state of knowledge in this field are in demand.

10.3390/ani12111402

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Artificial induction of superfetation in the European hare (Lepus europaeus)

European Journal of Wildlife Research (2010)

Roland Kozdrowski Michał Dzięcioł Ewa Stańczyk Małgorzata Ochota Piotr Sławuta

The aim of this study was to induce superfetation in hares. On day 37 or 39 of pregnancy, female hares were treated with GnRH analogue and artificially inseminated with fresh semen. Ultrasonographic examination showed that pregnant females had follicles on the ovary on 37 days of pregnancy. After a few days from insemination performed during pregnancy, all females gave birth to healthy young, and one of the females inseminated on day 37 of pregnancy gave birth to two healthy young 42 days after insemination performed during pregnancy (39 days post first delivery). The same female in the next year gave birth to two healthy young 43 days after second insemination that was performed on the day 37 during pregnancy. The obtained results confirm that superfetation is possible to occur in hares.

10.1007/s10344-010-0435-4

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Citations (1)

Characteristics of urethral and epididymal semen collected from domestic cats—A retrospective study of 214 cases

Theriogenology (2015)

Sylwia Prochowska Wojciech Niżański Małgorzata Ochota Agnieszka Partyka

Semen Analysis

10.1016/j.theriogenology.2015.08.005

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Citations (44)