정상인 혈청 194개와 결핵환자의 혈청 170개를 이용하여 결핵균 항원에 대한 반응성을 Western blotting으로 분석한 결과 모두 4가지의 반응형으로 나눈 수 있었다. 정상인 및 질병정도별 결핵환자와 치료경과 중 결핵환자의 반응형 빈도를 관찰한 결과 ii와 iii반응형은 활동형 결핵환자의 혈청에서만 관찰되었다. Western blotting 에서 ii와 iii반응형을 나타내는 환자를 활동성 결핵으로 간주하였을 경우 민감도는 32%이고 특이도는 100%이었다. 4종의 recording antigen과 임의 선택한 5종의 한무리 항체로 정제한 5종의 결핵균 항원을 이용한 dot-blotting 방법으로는 정상인과 결핵환자를 구분할 수 없었고 결핵의 혈청학적 진단에 있어서 민감도를 더 높이기 위해서는 앞으로 14-19KDa의 결핵균항원에 대한 recombinant antigen과 한무리항체를 집중적으로 개발하여야 할 것으로 사료되었다.
Background/Aims: Korean population has been suggested to be minimally exposed to hepatitis A virus (HAV) for recent 20 years. Therefore, hepatitis A epidemics may occur among medical students and medical personnels who care the asymptomatic patients with hepatitis A. We investigated the changes in anti-HAV IgG positive rate among the medical students for 10 years and the curren anti-HAV IgG positive rate among the medical personnels in Chinju. Methods: Serum anti-HAV IgG was measured using the commercially available HAVAB radioimmunoassay kit. The sera had been collected from 780 sophomore medical students during 1988-1997 and from 95 pediatric medical personnels at the Gyeongsang National University Hospital in December, 1997. Results: The anti-HAV IgG positive rate of the sophomore medical students was 87.5% in 1988, 86.7% in 1989 83.8% in 1990, 75% in 1991, 85% in 1992, 70% in 1993, 56.3% in 1994, 58.8% in 1995, 53.8% in 1996 and 38.8% in 1997. The anti-HAV IgG positive rate among the pediatric medical personnel (mean age ±S.D.; 26.4 ±3.1) was 65.3%. Conclusions: These suggest that the population susceptible to HAV exists among medical students and pediatric medical personnels. Therefore, we should se up the preventive modalities against hepatitis A virus infection among these high risk groups. (Kor J Gastroenterol 1999;33:520 - 526)
A λgtll expression library of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypeptide with a predicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homology searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.
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