Zhao You-shu

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Jiang Yongping

Institute of Hematology & Blood Diseases Hospital

Hongbo Zhang

University of Chinese Academy of Sciences

Hualan Chen

Harbin Veterinary Research Institute

Shumei Zhang

Heilongjiang Academy of Sciences

Guangqing Liu

Chinese Academy of Agricultural Sciences

Zuo QingShan

Ya Xueping

Zhigao Bu

Harbin Veterinary Research Institute

Yu Kangzhen

China Institute of Veterinary Drug Control

Pingjing Zhang

Renji Hospital

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Chinese Academy of Agricultural Sciences

Shanghai Veterinary Research Institute

Harbin Veterinary Research Institute

Innovation Team (China)

Yangzhou University

Gansu Agricultural University

Chinese Academy of Sciences

Nanjing Agricultural University

Shanghai Jiao Tong University

China Agricultural University

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Enhanced Protective Efficacy of Avian Influenza DNA Vaccine with Expressive Vector pCAGGS

Zhongguo nongye Kexue (2006)

Jiang Yongping Hongbo Zhang Zhigao Bu Chengjun Li Zhao You-shu

【Objective】 To improve the protective efficacy of H5 subtype avian influenza DNA vaccine,the HA gene of A/Goose/GuangDong/1/96(H5N1)[GD/1/96(H5N1)] was inserted into pCAGGS,which contains chickenβ-actin promoter,and was designated as pCAGGHA5.【Method】The monolayer 293T cells were transfected by pCAGGHA5 and pCIHA5,which contained HA gene of GD/1/96(H5N1).The HA protein expression was detected by indirect immunofluorescence assay 24-48 h after transfection and confirmed by western blotting analysis.To evaluate the vaccine efficacy,groups of 3-week-old SPF chickens were intramuscular inoculated with 100 μg or 10 μg of pCIHA5,pCAGGHA5,respectively.A group of chickens were injected with 200 μl PBS as control.Four-weeks after the single vaccination,all chickens were challenged with 100 LD50 of highly pathogenic GD/1/96(H5N1).【Results】 In vitro expression results indicated the gene in the pCAGGS vector was better expressed than that in the pCI vector.The chickens in the 100μg and 10μg pCAGGHA5 immunized groups were completely protected from virus challenge(no disease signs,no virus shedding and no deaths),and 100μg pCIHA5 immunized groups only provided partial protection,whilethe chickens in the pCIHA5 immunized group and the control group died within ten days.【Conclusion】 Results shown that expressive vector optimization enhanced the protective efficacy of H5 subtype avian influenza DNA vaccine.

Immunofluorescence

Shuttle vector

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Immune efficacy of the recombinant plasmids expressing HA gene of H1N1 and H3N2 subtype SIV in mice

Xumu shouyi xuebao (2007)

Yonghao Hu Shijun Bao Zhao You-shu Fengqin Wen Ya Xueping

BALB/c mice were inoculated with the recombinant plasmids(pCI-H1-HA,pCAGGS-H1-HA,pCI-H3-HA and pCAGGS-H3-HA) which included the HA gene of H1N1 and H3N2 subtype SIV to screen valuable vaccine candidate.The immune efficacy was evaluated by detecting HI antibody titers,analyzing weight changes of mice and examining virus titers in lungs.The results indicated that the constructed DNA vaccines induced immune responses in BALB/c mice.Meanwhile,by the statistical analysis of the weight changes of BALB/c mice,there were high significant differences between the immunization groups and the control(P0.01),and the DNA vaccines constructed with pCAGGS as the expression vector had better immune efficacy than that of DNA vaccines constructed with pCI(P0.05).

Antibody titer

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An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China

Virology (2011)

Pixi Yan Zhao You-shu Xu Zhang Dawei Xu Xiaoguang Dai

Embryonated

Veterinary virology

RNA virus

Strain (injury)

Flavivirus

10.1016/j.virol.2011.06.003

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Dynamic Alteration of CD8~+T Lymphocytes Induced by AIV HA Gene DNA Vaccine in SPF Chickens

Progress in Veterinary Medicine (2006)

Hongbo Zhang Jiang Yongping Quanying Wang Zhao You-shu Pingjing Zhang

SPF chickens were immunized with 100 μg DNA plasmid pCAGGoptiHA5,which encoding HA gene of AIV,and the CD8~+ T lymphocytes were detected by flow cytometer.In the first immunization,the amount of CD8~+ T lymphocytes in SPF chickens increased gradually and reached the peak three weeks after immunization.After challenge,the amount increased rapidly on three days,and maintained in a steady level in one week and then decreased to a low level in the next week.In the boost immunization,the increased speed of CD8~+T lymphocytes numbers are higher then that after first immunization,and reached the second peak on fourteen days later after boosting.After challenge,the number of CD8~+T lymphocytes increased slightly,and the decreased range is also lower than that in the first immunization two weeks after challenge.The amount of CD8~+T lymphocytes in control chickens hardly had no changes,but it had increasing trend after challenge,and all the control chickens died within one week after challenge.It demonstrated that HA gene vaccine pCAGGoptiHA5 could induce cellular immune responses successfully.

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