Joan Francesc Barquinero

Universitat Autònoma de Barcelona

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Leonardo Barrios

Universidad de Los Andes

Eric Grégoire

Federal Office for Radiation Protection

M.R. Caballı́n

Universitat Autònoma de Barcelona

Ursula Oestreicher

Federal Office for Radiation Protection

Alegría Montoro

Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento

Elizabeth A. Ainsbury

UK Health Security Agency

Gaëtan Gruel

Institut de Radioprotection et de Sûreté Nucléaire

Christina Beinke

Institut für Radiobiologie der Bundeswehr

Georgia I. Terzoudi

Institute of Nuclear & Radiological Sciences and Technology, Energy & Safety

Mònica Pujol-Canadell

Hospital General Universitario Gregorio Marañón

Cooperative Institutions

Universitat Autònoma de Barcelona

Institut de Radioprotection et de Sûreté Nucléaire

CEA Paris-Saclay - Etablissement de Fontenay-aux-roses

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Federal Office for Radiation Protection

Hospital Universitari i Politècnic La Fe

Centre National de la Recherche Scientifique

Inserm

Public Health England

Universitat de València

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Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents

Mutation Research/Genetic Toxicology and Environmental Mutagenesis (2013)

H. Romm Elizabeth A. Ainsbury Stephen Barnard Leonardo Barrios Joan Francesc Barquinero

Dicentric chromosome

Biodosimetry

Colcemid

Triage

Concordance

Automated method

Analyser

10.1016/j.mrgentox.2013.05.013

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Citations (85)

From double strand break (DBS) signalization to chromosome aberration formation

Mariona Peinado Ortiz Joan Francesc Barquinero

Chromosome aberration

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Main activities of the Latin American Network of Biological Dosimetry (LBDNet)

Marina Di Giorgio María Belén Vallerga A. Radl M. R. Taja M. Stuck Oliveira

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Uncertainty of fast biological radiation dose assessment for emergency response scenarios

International Journal of Radiation Biology (2016)

Elizabeth A. Ainsbury Manuel Higueras Pedro Puig Jochen Einbeck Daniel Samaga

Reliable dose estimation is an important factor in appropriate dosimetric triage categorization of exposed individuals to support radiation emergency response.Following work done under the EU FP7 MULTIBIODOSE and RENEB projects, formal methods for defining uncertainties on biological dose estimates are compared using simulated and real data from recent exercises.The results demonstrate that a Bayesian method of uncertainty assessment is the most appropriate, even in the absence of detailed prior information. The relative accuracy and relevance of techniques for calculating uncertainty and combining assay results to produce single dose and uncertainty estimates is further discussed.Finally, it is demonstrated that whatever uncertainty estimation method is employed, ignoring the uncertainty on fast dose assessments can have an important impact on rapid biodosimetric categorization.

Triage

Relevance

Uncertainty Quantification

10.1080/09553002.2016.1227106

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Citations (22)

Uncertainty calculation methods in dose assessment for dicentric chromosome assay

Figshare (2020)

Jorge E. González Joan Francesc Barquinero Bret Holladay Marina Di Giorgio Manuel Higueras

Purpose: To present the impact in coverage of different methods for Poisson confidence intervals and the impact in dose coverage of different uncertainty factors. A detailed explanation of the uncertainty sources in the Bayesian method is also presented. Materials and methods: The exact coverage of uncertainty Poisson confidence intervals and the dose uncertainty interval coverage were performed by simulations using R-based scripts. Results: The Poisson exact calibration interval via the Modified Crow and Gardner method resulted in coverage quite close to the nominal level of confidence; additionally, the method retains the shortest property of Crow and Gardner, and gains the property of a lower limit strictly increasing in the mean of dicentrics. The unlimited simultaneous calibration interval seems to be the method of choice to preserve the coverage at 95% under parametric and nonparametric conditions but is a conservative method. When samples came from a Poisson distribution, the ISO propagation of errors and Bayesian approaches seem to be the closest to the 95% coverage. Conclusions: The Modified Crow and Gardner method should be preferred over the Garwood method for Poisson exact confidence intervals. The unlimited simultaneous calibration interval did not lose its property to preserve the coverage at 95% applying a regression coverage factor of value 2.02 at the point of doses studied in the simulation.

Dicentric chromosome

10.6084/m9.figshare.11734566

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A Bayesian Poisson mixture model for model selection and dose estimation in biological retrospective dosimetry

HAL (Le Centre pour la Communication Scientifique Directe) (2019)

Sophie Ancelet Gaëtan Gruel Eric Grégoire Aurélie Vaurijoux Laurence Roy

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Interlaboratory comparison of dicentric chromosome assay using electronically transmitted images

Radiation Protection Dosimetry (2012)

Omar García Marina Di Giorgio María Belén Vallerga A. Radl M. R. Taja

The bottleneck in data acquisition during biological dosimetry based on a dicentric assay is the need to score dicentrics in a large number of lymphocytes. One way to increase the capacity of a given laboratory is to use the ability of skilled operators from other laboratories. This can be done using image analysis systems and distributing images all around the world. Two exercises were conducted to test the efficiency of such an approach involving 10 laboratories. During the first exercise (E1), the participant laboratories analysed the same images derived from cells exposed to 0.5 and 3 Gy; 100 images were sent to all participants for both doses. Whatever the dose, only about half of the cells were complete with well-spread metaphases suitable for analysis. A coefficient of variation (CV) on the standard deviation of ∼15 % was obtained for both doses. The trueness was better for 3 Gy (0.6 %) than for 0.5 Gy (37.8 %). The number of estimated doses classified as satisfactory according to the z-score was 3 at 0.5 Gy and 8 at 3 Gy for 10 dose estimations. In the second exercise, an emergency situation was tested, each laboratory was required to score a different set of 50 images in 2 d extracted from 500 downloaded images derived from cells exposed to 0.5 Gy. Then the remaining 450 images had to be scored within a week. Using 50 different images, the CV on the estimated doses (79.2 %) was not as good as in E1, probably associated to a lower number of cells analysed (50 vs. 100) or from the fact that laboratories analysed a different set of images. The trueness for the dose was better after scoring 500 cells (22.5 %) than after 50 cells (26.8 %). For the 10 dose estimations, the number of doses classified as satisfactory according to the z-score was 9, for both 50 and 500 cells. Overall, the results obtained support the feasibility of networking using electronically transmitted images. However, before its implementation some issues should be elucidated, such as the number and resolution of the images to be sent, and the harmonisation of the scoring criteria. Additionally, a global website able to be used for the different regional networks, like Share Points, will be desirable to facilitate worldwide communication.

Dicentric chromosome

Coefficient of variation

Densitometer

10.1093/rpd/ncs139

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Citations (19)

Cambiar la dirección de centros para mejorar el sistema educativo

Organización y gestión educativa: Revista del Fórum Europeo de Administradores de la Educación (2016)

Joan Francesc Barquinero

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RENEB biodosimetry intercomparison analyzing translocations by FISH

International Journal of Radiation Biology (2016)

Joan Francesc Barquinero Christina Beinke Mireia Borràs Iwona Buraczewska F. Darroudi

Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium.Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates.Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively.Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.

Biodosimetry

10.1080/09553002.2016.1222092

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Analysis of Translocations in Stable Cells and their Implications in Retrospective Biological Dosimetry

Radiation Research (2004)

P. Rodríguez Alegría Montoro Joan Francesc Barquinero M.R. Caballı́n I. Villaescusa

Rodríguez, P., Montoro, A., Barquinero, J. F., Caballín, M. R., Villaescusa, I. and Barrios, L. Analysis of Translocations in Stable Cells and their Implications in Retrospective Biological Dosimetry. Radiat. Res. 162, 31–38 (2004).The aim of the present study was to evaluate the influence of the exclusion of cells with unstable aberrations in the elaboration of dose–effect curves for translocations and their implications in biological dosimetry of past exposures. To establish dose–effect curves, peripheral blood samples were irradiated with 60Co γ rays at ten different doses and the yield of translocations analyzed by FISH was considered in all cells and in stable cells (those without dicentrics, acentrics or rings). To discriminate transmissible translocations, the dose– effect curve for total apparently simple translocations in stable cells was chosen as the reference. In stable cells, dose– effect curves for apparently simple translocations without pseudosimple and complex-derived one-way patterns, t(Ab)t(Ba) and total translocations were obtained. None of these curves differed from the reference curve. When all cells were considered, only the curve for total translocations was significantly different from the reference curve. From the results obtained it can be concluded that the use of dose–effect curves for apparently simple translocations in stable cells and in all cells will give similar dose estimates in retrospective biological dosimetry studies. However, the use of dose–effect curves for total translocations in all cells will lead to underestimations of the dose mainly at high doses.

10.1667/rr3198

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Citations (20)