Jason Gorman

National Institute of Allergy and Infectious Diseases

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Peter D. Kwong

Columbia University

121

John R. Mascola

National Institute of Allergy and Infectious Diseases

Tongqing Zhou

National Institute of Allergy and Infectious Diseases

Baoshan Zhang

National Institute of Allergy and Infectious Diseases

Lawrence Shapiro

Columbia University

Nicole A. Doria‐Rose

National Institute of Allergy and Infectious Diseases

Gwo‐Yu Chuang

National Institute of Allergy and Infectious Diseases

Mark K. Louder

National Institute of Allergy and Infectious Diseases

Yaroslav Tsybovsky

Leidos (United States)

Reda Rawi

National Institute of Allergy and Infectious Diseases

Cooperative Institutions

National Institutes of Health

389

National Institute of Allergy and Infectious Diseases

337

University of Pennsylvania

National Cancer Institute

Institut de Recherche Vaccinale

Columbia University

Harvard University

Duke University

Duke Medical Center

Scripps Research Institute

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

SOIL DEVELOPMENT ON A MOUNTAINTOP REMOVAL MINE IN SOUTHERN WEST VIRGINIA

Reclamation Sciences (2000)

Karen A. Thomas J. Sencindiver Jeff Skousen Jason Gorman

West virginia

10.21000/jasmr00010546

Cite

Citations (19)

Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage

Cell Reports (2025)

Rosemarie D. Mason Baoshan Zhang Nicholas C. Morano Chen‐Hsiang Shen Krisha McKee

Apex (geometry)

Lineage (genetic)

10.1016/j.celrep.2024.115223

Cite

Citations (0)

Potent Neutralizing Antibodies Directed to Multiple Epitopes on SARS-CoV-2 Spike

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Lihong Liu Pengfei Wang Manoj S. Nair Jian Yu Micah Rapp

Abstract The SARS-CoV-2 pandemic rages on with devasting consequences on human lives and the global economy 1,2 . The discovery and development of virus-neutralizing monoclonal antibodies could be one approach to treat or prevent infection by this novel coronavirus. Here we report the isolation of 61 SARS-CoV-2-neutralizing monoclonal antibodies from 5 infected patients hospitalized with severe disease. Among these are 19 antibodies that potently neutralized the authentic SARS-CoV-2 in vitro , 9 of which exhibited exquisite potency, with 50% virus-inhibitory concentrations of 0.7 to 9 ng/mL. Epitope mapping showed this collection of 19 antibodies to be about equally divided between those directed to the receptor-binding domain (RBD) and those to the N-terminal domain (NTD), indicating that both of these regions at the top of the viral spike are immunogenic. In addition, two other powerful neutralizing antibodies recognized quaternary epitopes that are overlapping with the domains at the top of the spike. Cryo-electron microscopy reconstructions of one antibody targeting RBD, a second targeting NTD, and a third bridging two separate RBDs revealed recognition of the closed, “all RBD-down” conformation of the spike. Several of these monoclonal antibodies are promising candidates for clinical development as potential therapeutic and/or prophylactic agents against SARS-CoV-2.

Epitope mapping

Conformational epitope

10.1101/2020.06.17.153486

Cite

Citations (61)

Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop

Immunity (2017)

Evan M. Cale Jason Gorman Nathan A. Radakovich Ema T. Crooks Keiko Osawa

Apex (geometry)

V3 loop

Trimer

10.1016/j.immuni.2017.04.011

Cite

Citations (83)

Ontogeny‐based immunogens for the induction of V2‐directed HIV broadly neutralizing antibodies

Immunological Reviews (2017)

Penny L. Moore Jason Gorman Nicole A. Doria‐Rose Lynn Morris

Summary The development of a preventative HIV vaccine able to elicit broadly neutralizing antibodies ( bNA bs) remains a major challenge. Antibodies that recognize the V2 region at the apex of the HIV envelope trimer are among the most common bNA b specificities during chronic infection and many exhibit remarkable breadth and potency. Understanding the developmental pathway of these antibodies has provided insights into their precursors, and the viral strains that engage them, as well as defined how such antibodies mature to acquire breadth. V2‐apex bNA bs are derived from rare precursors with long anionic CDR H3s that are often deleted in the B cell repertoire. However, longitudinal studies suggest that once engaged, these precursors contain many of the structural elements required for neutralization, and can rapidly acquire breadth through moderate levels of somatic hypermutation in response to emerging viral variants. These commonalities in the precursors and mechanism of neutralization have enabled the identification of viral strains that show enhanced reactivity for V2 precursors from multiple donors, and may form the basis of germline targeting approaches. In parallel, new structural insights into the HIV trimer, the target of these quaternary antibodies, has created invaluable new opportunities for ontogeny‐based immunogens designed to select for rare V2‐ bNA b precursors, and drive them toward breadth.

Antibody Repertoire

HIV vaccine

Affinity maturation

10.1111/imr.12501

Cite

Citations (32)

Development of Neutralization Breadth against Diverse HIV‐1 by Increasing Ab–Ag Interface on V2

Advanced Science (2022)

Nan Gao Yanxin Gai Lina Meng Chu Wang Wei Wang

Abstract Understanding maturation pathways of broadly neutralizing antibodies (bnAbs) against HIV‐1 can be highly informative for HIV‐1 vaccine development. A lineage of J038 bnAbs is now obtained from a long‐term SHIV‐infected macaque. J038 neutralizes 54% of global circulating HIV‐1 strains. Its binding induces a unique “up” conformation for one of the V2 loops in the trimeric envelope glycoprotein and is heavily dependent on glycan, which provides nearly half of the binding surface. Their unmutated common ancestor neutralizes the autologous virus. Continuous maturation enhances neutralization potency and breadth of J038 lineage antibodies via expanding antibody‐Env contact areas surrounding the core region contacted by germline‐encoded residues. Developmental details and recognition features of J038 lineage antibodies revealed here provide a new pathway for elicitation and maturation of V2‐targeting bnAbs.

Lineage (genetic)

Immune escape

Simian immunodeficiency virus

10.1002/advs.202200063

Cite

Citations (5)

Synthesis of Bullatacin

Synfacts (2007)

Brian L. Pagenkopf Hong‐Yi Zhao Jason Gorman

Bullatacin is a cytotoxic annonaceous acetogenin. Pagenkopf and co-workers use Mukaiyama’s cobalt-catalyzed oxidative cyclization of 5-hydroxy-1-alkenes to prepare the bis(tetrahydrofuran) core and metal acetylide additions to aldehydes to append the remainder of the molecule.

Acetylide

Acetogenin

Tetrahydrofuran

10.1055/s-2006-955758

Cite

Citations (0)

Design of soluble HIV-1 envelope trimers free of covalent gp120-gp41 bonds with prevalent native-like conformation

Cell Reports (2024)

Peng Zhang Jason Gorman Yaroslav Tsybovsky Maolin Lu Qingbo Liu

Soluble HIV-1 envelope (Env) trimers may serve as effective vaccine immunogens. The widely utilized SOSIP trimers have been paramount for structural studies, but the disulfide bond they feature between gp120 and gp41 constrains intersubunit mobility and may alter antigenicity. Here, we report an alternative strategy to generate stabilized soluble Env trimers free of covalent gp120-gp41 bonds. Stabilization was achieved by introducing an intrasubunit disulfide bond between the inner and outer domains of gp120, defined as interdomain lock (IDL). Correctly folded IDL trimers displaying a native-like antigenic profile were produced for HIV-1 Envs of different clades. Importantly, the IDL design abrogated CD4 binding while not affecting recognition by potent neutralizing antibodies to the CD4-binding site. By cryoelectron microscopy, IDL trimers were shown to adopt a closed prefusion configuration, while single-molecule fluorescence resonance energy transfer documented a high prevalence of native-like conformation. Thus, IDL trimers may be promising candidates as vaccine immunogens.

Antigenicity

Trimer

10.1016/j.celrep.2024.114518

Cite

Citations (2)

Structure and immune recognition of trimeric pre-fusion HIV-1 Env

Carolina Digital Repository (University of North Carolina at Chapel Hill) (2014)

Marie Pancera Tongqing Zhou Aliaksandr Druz Ivelin S. Georgiev Cinque Soto

Immune Recognition

10.17615/bt86-bx50

Cite

Citations (0)

Potent SARS-CoV-2 Neutralizing Antibodies Directed Against Spike N-Terminal Domain Target a Single Supersite

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Gabriele Cerutti Yicheng Guo Tongqing Zhou Jason Gorman Myungjin Lee

Summary Numerous antibodies that neutralize SARS-CoV-2 have been identified, and these generally target either the receptor-binding domain (RBD) or the N-terminal domain (NTD) of the viral spike. While RBD-directed antibodies have been extensively studied, far less is known about NTD-directed antibodies. Here we report cryo-EM and crystal structures for seven potent NTD-directed neutralizing antibodies in complex with spike or isolated NTD. These structures defined several antibody classes, with at least one observed in multiple convalescent donors. The structures revealed all seven antibodies to target a common surface, bordered by glycans N 17, N 74, N 122, and N 149. This site – formed primarily by a mobile β-hairpin and several flexible loops – was highly electropositive, located at the periphery of the spike, and the largest glycan-free surface of NTD facing away from the viral membrane. Thus, in contrast to neutralizing RBD-directed antibodies that recognize multiple non-overlapping epitopes, potent NTD-directed neutralizing antibodies target a single supersite.

spike protein

Epitope mapping

Single-domain antibody

10.1101/2021.01.10.426120

Cite

Citations (46)