Md Shahinozzaman

University of Maryland, College Park

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Shinkichi Tawata

University of the Ryukyus

Diana N. Obanda

University of Maryland, College Park

Mustafa Abul Kalam Azad

University of Rajshahi

Milad Ashrafizadeh

Shandong First Medical University

Samnhita Raychaudhuri

University of Maryland, College Park

Ali Zarrabi

Yuan Ze University

Mohammad A. Halim

Kennesaw State University

Si Fan

University of Maryland, College Park

Takahiro Ishii

University of the Ryukyus

Masoud Najafi

Kermanshah University of Medical Sciences

Cooperative Institutions

University of Dhaka

Tehran University of Medical Sciences

University of the Ryukyus

University of Rajshahi

Shahid Beheshti University of Medical Sciences

Université Moulay Ismail de Meknes

Instituto Superior da Maia

University of Maryland, College Park

University of the Ryukyus University Hospital

Islamic Azad University Medical Branch of Tehran

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Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii

Zeitschrift für Naturforschung C (2019)

Md Shahinozzaman Takahiro Ishii Mohammad A. Halim Md. Amzad Hossain Tofazzal Islam

Abstract Medicinal plants belonging to the genus Ardisia are traditionally used to cure various human diseases including inflammation and cancer. This study aimed to purify and characterize cytotoxic and anti-inflammatory compounds from Ardisia sieboldii leaves. Bioassay-guided chromatographic analyses yielded three compounds, 2-methyl-5-(8 Z -heptadecenyl) resorcinol ( 1 ), 5-(8 Z -heptadecenyl) resorcinol ( 2 ), and ardisiaquinone A ( 3 ), whereas liquid chromatography–electrospray ionisation–mass spectrometry chemical profiling revealed the presence of diverse resorcinol and alkylbenzoquinone derivatives in cytotoxic 70% methanol extracts. Chemical structures of 1 – 3 were confirmed by spectroscopic methods including 1 H NMR (nuclear magnetic resonance), 13 C NMR, and electrospray ionisation–mass spectrometry. Compounds 1 and 2 were purified from A. sieboldii for the first time, and all three compounds showed cytotoxicity against a panel of cancer cell lines and brine shrimps in a dose-response manner. Among them, compound 2 exhibited the highest cytotoxicity on cancer cells (IC 50 values of 8.8–25.7 μM) as well as on brine shrimps (IC 50 value of 5.1 μM). Compounds 1 – 3 exhibited anti-inflammatory effects through inhibiting protein denaturation (IC 50 values of 5.8–9.6 μM), cyclooxygenase-2 activity (IC 50 values of 34.5–60.1 μM), and nitrite formation in RAW 264.7 cells. Cytotoxic and anti-inflammatory activities of 1 – 3 demonstrated in this study deserve further investigation for considering their suitability as candidates or leads to develop anticancer and anti-inflammatory drugs.

Resorcinol

Brine shrimp

Anti-inflammatory

10.1515/znc-2019-0114

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Citations (12)

Crosstalk of Long Non-coding RNAs and EMT: Searching the Missing Pieces of an Incomplete Puzzle for Lung Cancer Therapy

Current Cancer Drug Targets (2021)

Milad Ashrafizadeh Md Shahinozzaman Sima Orouei Vahideh Zarrin Kiavash Hushmandi

Lung cancer has the first place among cancer-related deaths worldwide and demands novel strategies in the treatment of this life-threatening disorder. The aim of this review is to explore the regulation of epithelial-to-mesenchymal transition (EMT) by long non-coding RNAs (lncRNAs) in lung cancer.LncRNAs can be considered as potential factors for targeting in cancer therapy, since they regulate a bunch of biological processes, e.g. cell proliferation, differentiation and apoptosis. The abnormal expression of lncRNAs occurs in different cancer cells. On the other hand, epithelial-to-mesenchymal transition (EMT) is a critical mechanism participating in migration and metastasis of cancer cells.Different databases, including Google Scholar, Pubmed and Science direct, were searched for collecting articles using keywords such as "LncRNA", "EMT", and "Lung cancer".There are tumor-suppressing lncRNAs that can suppress EMT and metastasis of lung cancer cells. Expression of such lncRNAs undergoes down-regulation in lung cancer progression and restoring their expression is of importance in suppressing lung cancer migration. There are tumor- promoting lncRNAs triggering EMT in lung cancer and enhancing their migration.LncRNAs are potential regulators of EMT in lung cancer, and targeting them, both pharmacologically and genetically, can be of importance in controlling the migration of lung cancer cells.

Crosstalk

10.2174/1568009621666210203110305

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Citations (33)

Cytotoxic Desulfated Saponin from Holothuria atra Predicted to Have High Binding Affinity to the Oncogenic Kinase PAK1: A Combined In Vitro and In Silico Study

Scientia Pharmaceutica (2018)

Md Shahinozzaman Takahiro Ishii Ryo Takano Mohammad A. Halim Md. Amzad Hossain

Sea cucumbers have long been utilized in foods and Asiatic folk medicines for their nutritive and health benefits. Herein, three sea cucumber species were investigated and Holothuria atra showed the highest cytotoxicity among these. Next, a desulfated saponin, desulfated echinoside B (DEB), was purified from H. atra through bioassay-guided fractionation. LC-ESI-MS (Liquid chromatography-electrospray ionization mass spectrometry) analysis also showed H. atra to be a rich source of saponins. DEB showed cytotoxicity on cancer cells with IC50 values of 0.5⁻2.5 µM, and on brine shrimps with an IC50 value of 9.2 µM. In molecular docking studies, DEB was found to bind strongly with the catalytic domain of PAK1 (p21-activated kinase 1) and it showed binding energy of -8.2 kcal/mol compared to binding energy of -7.7 kcal/mol for frondoside A (FRA). Both of them bind to the novel allosteric site close to the ATP-binding cleft. Molecular dynamics (MD) simulation demonstrated that DEB can form a more stable complex with PAK1, remaining inside the allosteric binding pocket and forming the maximum number of hydrogen bonds with the surrounding residues. Moreover, important ligand binding residues were found to be less fluctuating in the DEB-PAK1 complex than in the FRA-PAK1 complex throughout MD simulation. Our experimental and computational studies showed that both DEB and FRA can act as natural allosteric PAK1 inhibitors and DEB appeared to be more promising than FRA.

Docking (animal)

IC50

10.3390/scipharm86030032

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Citations (19)

Direct Organogenesis and Plant Regeneration from Cotyledons of a Multipurpose Tree, Acacia mangium Willd.

Current Trends in Biotechnology and Pharmacy (2013)

Md Shahinozzaman M. O. Faruq Mahbuba Ferdous Mustafa Abul Kalam Azad Md. Nurul Amin

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with IBA (8.0 μM) was found best for rooting of in vitro shoots. The rooted plantlets were then transferred to small pots containing a sterilized mixture of sand, garden soil and compost (1:1:1) for acclimatization where plantlets grew well with 65% survival rate.

Organogenesis

Acacia mangium

Cotyledon

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Citations (8)

Cancer and SOX proteins: New insight into their role in ovarian cancer progression/inhibition

Pharmacological Research (2020)

Milad Ashrafizadeh Shahram Taeb Kiavash Hushmandi Sima Orouei Md Shahinozzaman

Transcription

10.1016/j.phrs.2020.105159

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Citations (30)

In vitro propagation of a medicinal plant - mango ginger (Curcuma amada Roxb.).

International Journal of Biosciences (2012)

Mahbuba Ferdous Md Shahinozzaman M. O. Faruq Shilpi Paul Mustafa Abul Kalam Azad

This paper describes efficient in vitro propagation protocol of Curcuma amada Roxb., an important aromatic zingiberaceous plant in the countries of Indian subcontinent, using rhizome explants. Rhizome tip explants showed better response than rhizome bud explants in terms of shoot proliferation and hence excised rhizome tip explants were inoculated aseptically on Murashige and Skoog’s (MS) medium supplemented with different 6Benzyl adenine (BA) or 6-furfurylamino purine (Kn) concentrations (0.0, 2.0, 4.0, 6.0, 8.0, 10.0 µM) alone or in combinations with α- Naphthalene acetic acid (NAA) and Indole-3-butyric acid (IBA) (1.0, 1.5, 2.0 µM). Optimum proliferation was obtained on MS medium having 8.0 µM BA + 1.0 µM NAA. Shoot proliferation was maximal (99.8%) with 10.6 ± 0.26 shoots per explant after 6 weeks of culture. The successfully proliferated shoots with an average height of 38 cm were transferred to rooting medium augmented with NAA and IBA at four different concentrations (2.0, 4.0, 6.0, 8.0 µM) and 4.0 µM IBA was found to be best for in vitro adventitious rooting. Rooted plantlets were then transferred to plastic cups containing autoclaved garden soil and compost (1:1), gradually acclimatized and finally transferred to the field condition with 80% survival rate.

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A review on chemistry, source and therapeutic potential of lambertianic acid

Zeitschrift für Naturforschung C (2021)

Md Shahinozzaman M. Sayedul Islam Bristy Basak Arifa Sultana Rashiduzzaman Emran

Lambertianic acid (LA) is a diterpene bioactive compound mainly purified from different species of Pinus. It is an optical isomer of another natural compound daniellic acid and was firstly purified from Pinus lambertiana. LA can be synthesized in laboratory from podocarpic acid. It has been reported to have potential health benefits in attenuating obesity, allergies and different cancers including breast, liver, lung and prostate cancer. It exhibits anticancer properties through inhibiting cancer cell proliferation and survival, and inducing apoptosis, targeting major signalling components including AKT, AMPK, NFkB, COX-2, STAT3, etc. Most of the studies with LA were done using in vitro models, thus warranting future investigations with animal models to evaluate its pharmacological effects such as antidiabetic, anti-inflammatory and neuroprotective effects as well as to explore the underlying molecular mechanisms and toxicological profile. This review describes the chemistry, source, purification and therapeutic potentials of LA and it can therefore be a suitable guideline for any future study with LA.

10.1515/znc-2020-0267

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Citations (2)

Artepillin C: A comprehensive review of its chemistry, bioavailability, and pharmacological properties

Fitoterapia (2020)

Md Shahinozzaman Bristy Basak Rashiduzzaman Emran Patricia Rozario Diana N. Obanda

Repurposing

Propolis

Baccharis

10.1016/j.fitote.2020.104775

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Citations (45)

The Vegetable ‘Kale’ Protects against Dextran-Sulfate-Sodium-Induced Acute Inflammation through Moderating the Ratio of Proinflammatory and Anti-Inflammatory LPS-Producing Bacterial Taxa and Augmenting the Gut Barrier in C57BL6 Mice

Nutrients (2023)

Samnhita Raychaudhuri Md Shahinozzaman Ujjwol Subedi Si Fan Opeyemi Ogedengbe

Kale (

Proinflammatory cytokine

10.3390/nu15143222

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Citations (1)

Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity

Microorganisms (2021)

Md Shahinozzaman Samnhita Raychaudhuri Si Fan Diana N. Obanda

Kale (Brassica oleracea var. acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli. We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice. We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2. Like other cruciferous vegetables, kale attenuated HFD-induced inflammation. In addition, kale modulated HFD-induced changes in cecal microbiota composition. The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition. Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli). Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia. Within Actinobacteria, HFKV particularly increased Coriobacteriales/Coriobacteriaceae about four-fold compared to the HFD (p < 0.05). Among Bacteroidia, HFKV increased the species Bacteroides thetaiotaomicron by over two-fold (p = 0.05) compared to the HFD. This species produces plant polysaccharide digesting enzymes. Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism. Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.

Clostridia

10.3390/microorganisms9020238

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Citations (25)