Anne Katrin Werenskiold

Max Planck Institute of Biochemistry

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R Klemenz

University Hospital of Zurich

Brigitte Rupp

Ludwig-Maximilians-Universität München

Joana Danescu

Bettina POETSCH

University of Tübingen

Jörg Schmidt

Philipps University of Marburg

Sylvia Hoffmann

University of Wales Trinity Saint David

Ute Rößler

Federal Office for Radiation Protection

Ursula Lorenz

Institute of Groundwater Ecology

Michal Linial

Hebrew University of Jerusalem

Luís Serrano

Institució Catalana de Recerca i Estudis Avançats

Cooperative Institutions

Max Planck Institute of Biochemistry

University Hospital of Zurich

Ludwig-Maximilians-Universität München

University of Zurich

Technical University of Munich

Charité - Universitätsmedizin Berlin

University of Bern

Ludwig Cancer Research

Thermo Fisher Scientific (Germany)

Thermo Fisher Scientific (United States)

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Serum- and oncoprotein-mediated induction of a gene with sequence similarity to the gene encoding carcinoembryonic antigen.

Proceedings of the National Academy of Sciences (1989)

R Klemenz Sylvia Hoffmann Anne Katrin Werenskiold

We describe the molecular identification of a gene, designated T1, whose expression in mouse NIH 3T3 cells is strongly induced by the Ha-ras(EJ) and v-mos oncogenes and by serum. The T1 gene encodes a 38.5-kDa protein, as predicted from its primary sequence and shown by in vitro translation. The protein was processed at its amino terminus and extensively modified by N-linked glycosylation in vitro in the presence of microsomal vesicles. Sequence comparison of T1 with the MIPSX data base (Max-Planck-Institut für Biochemie, Martinsried/Munich) revealed similarity to the human carcinoembryonic antigen, a tumor marker which is overexpressed in colon adenocarcinomas and in fetal tissues. Considerable sequence similarity has also been observed to the short conserved region of other proteins which, like carcinoembryonic antigen, are encoded by members of the immunoglobulin gene superfamily.

Carcinoembryonic antigen

10.1073/pnas.86.15.5708

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Citations (167)

Cloning and expression of a β tubulin gene of Physarum polycephalum

European Journal of Biochemistry (1988)

Anne Katrin Werenskiold Bettina POETSCH Finn Haugli

A β tubulin gene of Physarum polycephalum has been isolated from a genomic library in the phage EMBL4. Southern‐blot hybridization to genomic DNA indicates that the cloned DNA is derived from the betB1 locus of the β tubulin gene family. A tubulin‐specific subfragment of the phage DNA was used as a hybridization probe to construct a restriction map of the betB1 locus. The probe consisted of the almost complete coding region of the 5′ half of the tubulin gene, interrupted by one intron. The derived amino acid sequence of this subclone deviates from the protein sequence for Physarum amoebal β tubulin (amino acids 4–207) in two of 207 amino acids. We used both recA and recBC sbcB bacterial host strains, which have been recommended for cloning of instability‐conferring sequences of the Physarum genome, but were unable to subclone the 3′ part of the gene from the phage DNA. Primer‐extension analysis indicates that the betB gene is expressed in the vegetatively proliferating amoebal and plasmodial stages of the life cycle as well as in differentiating (sporulating) plasmodia.

Physarum

Southern blot

genomic DNA

10.1111/j.1432-1033.1988.tb14125.x

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Citations (12)

Secreted and Membrane-Bound Isoforms of T1, an Orphan Receptor Related to IL-1-Binding Proteins, Are Differently Expressed in Vivo

Developmental Biology (1995)

Ute Rößler Elisabeth Thomassen Lothar Hütner Stefan Baier Joana Danescu

Orphan receptor

Cell surface receptor

10.1006/dbio.1995.1063

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Citations (52)

Transcription of the Interleukin-1 Receptor-related T1 Gene Is Initiated at Different Promoters in Mast Cells and Fibroblasts

Journal of Biological Chemistry (1996)

Thomas Gächter Anne Katrin Werenskiold R Klemenz

The delayed early serum response gene T1 encodes glycoproteins of the immunoglobulin superfamily with significant sequence similarity to the type 1 interleukin-1 receptor. The T1 gene is transcribed in fibroblasts into an abundant 2.7-kilobase (kb) and a rare 5-kb mRNA in response to proliferation-inducing stimuli. It gives predominantly rise to the longer transcript in the bone marrow of adult mice and in cultured mast cells. Alternative 3' processing is responsible for the two mRNA forms. The short transcript encodes a secreted protein with marked similarity to the extracellular domain of the interleukin-1 receptor, whereas the long mRNA is translated into a protein with an additional putative transmembrane and an intracellular domain. Here we demonstrate that T1 transcription in mast cells and fibroblasts initiates at two different start sites which are 10.5 kb apart. The alternative first exons are both spliced to exon 2 which contains the translation start site. Northern blot analysis and primer extension experiments revealed that promoter usage is strictly cell type-specific. T1 transcription in mast cells is initiated exclusively at the distal promoter, whereas in fibroblasts both the short and the long T1 mRNA start at the proximal promoter. Two GATA-1 elements were identified in the 5'-flanking region of the mast cell-specific distal exon 1.

Transcription

Interleukin 33

10.1074/jbc.271.1.124

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Citations (94)

Photomorphogenesis in Physarum polycephalum

European Journal of Biochemistry (1989)

Bettina POETSCH Thomas Schreckenbach Anne Katrin Werenskiold

Photo‐induced fruiting‐body formation of the slime mold Physarum polycephalum can be divided into two stages. The first stage (I) starts with the beginning of illumination (O h) and ends with the formation of nodules (12 h). The second stage (II) is characterized by culmination, sporangiophore formation and melanization of sporangiophore heads (13–17 h). We investigated the expression of actin, α‐ and β‐tubulin during this differentiation process using Northern blot analysis and run‐off transcription in isolated nuclei. Whereas actin mRNA is irreversibly lost during stage I, we observed two peaks in mRNA concentration for α‐tubulin and one peak for that of β‐tubulin during stage I and a coordinated α/β‐tubulin mRNA induction during stage II. Stage II induction appears to be related to a presporangial mitosis. Transcriptional activity of the three genes studied shows two maxima, namely one in the middle of stage I and the other at the end of stage II. Our data suggest that the expression of the three cytoskeletal proteins investigated follows a distinct temporal pattern comprising changes in both mRNA synthesis and decay. We also propose a novel function of tubulin in Physarum which is not related to mitosis.

Physarum

Alpha (finance)

Northern blot

BETA (programming language)

10.1111/j.1432-1033.1989.tb14531.x

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Citations (7)

High-Level Expression of the IL-1 Receptor Related T1 Receptor in Insect Cells

Biochemical and Biophysical Research Communications (1995)

B. Rupp Ute Rößler Marianne Löwel Anne Katrin Werenskiold

Cell surface receptor

Receptor expression

10.1006/bbrc.1995.2664

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Citations (4)

Discordant effects of activator protein-1 transcription factor on gene regulation, invasion, and metastasis in spontaneous, radiation-induced, andfos-induced osteosarcomas

Molecular Carcinogenesis (1998)

Brigitte Rupp Ursula Lorenz Jörg Schmidt Anne Katrin Werenskiold

Bone cells are a prime target for the biological function of the fos/jun (activating protein-1 (AP-1)) transcription factor complex. Deregulated expression of c-fos or v-fos in bone cells induces tumorigenicity and the formation of non-metastatic osteosarcomas. In contrast, fos oncogenes transform fibroblasts to an invasive phenotype accompanied by the expression of various invasion- and metastasis-associated genes. Here we compared the expression of AP-1-dependent genes and AP-1 activity in cell lines from fos-induced, radiation-induced, and spontaneous osteosarcomas. We showed that the presence of high AP-1 activity was not sufficient for the induction of invasion- and metastasis-associated AP-1-dependent genes in transformed bone cells. Further, we identified the collagenase I and stromelysin 1 gene promoters as suitable tools for the analysis of other factors regulating metastatic progression of osteosarcoma.

Transcription

10.1002/(sici)1098-2744(199810)23:2<69::aid-mc3>3.0.co;2-e

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Citations (3)

Tumor-Associated Overexpression of the Soluble T1-S Receptor in Lymph Node–Negative Breast Cancer

Diagnostic Molecular Pathology (2000)

Anne Katrin Werenskiold Dieter Prechtel Nadia Harbeck Heinz Höfler

The oncogene-inducible secreted T1-S glycoprotein is overexpressed in invasive breast carcinomas in mice. As yet, nothing is known about the expression of T1-S in spontaneously occurring human cancers. A report follows on the overexpression of T1-S mRNA in 67% of primary invasive lymph node-negative breast carcinomas (31 of 46 patients) as determined by quantitative reverse transcriptase polymerase chain reaction. Overexpression of T1-S mRNA was independent of the tumor size, the histologic tumor type, and the estrogen-and progesterone-receptor status but was associated with high to moderate differentiation of the tumors (G , G2). T1-S mRNA levels were low to nondetectable in resting normal mammary tissue and benign fibrocystic disease of the breast. Immunohistochemistry confirmed a low to moderate T1 immunoreactivity in epithelial cells of resting mammary tissue and benign fibrocystic disease and highly variable levels of T1 immunoreactivity in breast carcinoma cells. Kaplan-Meier analysis of disease-free survival during a median observation period of 61 months revealed a trend toward a reduced relapse rate and an extended relapse-free survival period for T1-S mRNA--overexpressing breast carcinomas. It is concluded that overexpression of T1-S receptor in lymph node-negative breast cancer may be a potential indicator for tumors with a low metastatic potential.

Progesterone receptor

Breast carcinoma

10.1097/00019606-200003000-00005

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Citations (5)

Post‐transcriptional inhibition of the interleukin‐1 binding protein B15R of Vaccinia virus after coexpression of the related T1 protein

FEBS Letters (1995)

Joana Danescu Anne Katrin Werenskiold

The interleukin‐1 binding B15R protein of Vaccinia virus and murine T1 are related extracellular glycoproteins with similarity to the extracellular domain of interleukin‐1 receptors. In cells infected with a recombinant Vaccinia virus directing the overexpression of T1, production of the endogenous viral B15R protein is abrogated. T1 synthesis specifically interferes with the production of B15R, but not of other secretory viral proteins. Inhibition of B15R expression occurs at the posttranscriptional level, is exerted in trans and requires the presence of T1 protein in the infected cell. These results suggest a common maturation pathway for the B15R and T1 proteins which might also apply to other members of the interleukin‐1 receptor family.

10.1016/0014-5793(95)00542-h

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Citations (1)

Induction of a mitogen-responsive gene after expression of the Ha-ras oncogene in NIH 3T3 fibroblasts.

Molecular and Cellular Biology (1989)

Anne Katrin Werenskiold Sylvia Hoffmann R Klemenz

A cDNA clone, T1, has been isolated whose corresponding mRNA was transiently expressed at highly elevated levels after conditional expression of the Ha-ras(EJ) gene and after mitogenic activation of quiescent NIH 3T3 cells. Glucocorticoid hormone stimulated substantial T1 expression as well but only in proliferating cells. At least two different signaling pathways participate in the regulation of the T1 gene: a protein kinase C-dependent signal is involved in the response of proliferating NIH 3T3 cells to glucocorticoid in the absence but not the presence of p21ras, whereas a protein kinase C-independent mechanism mediates the response to serum factors. Treatment of cells with the protein kinase inhibitor 2-aminopurine blocked induction of expression of the T1 gene. T1 mRNA accumulation is regulated at the transcriptional level.

3T3 cells

10.1128/mcb.9.11.5207

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Citations (78)