Stefano Leporatti

Istituto Nanoscienze

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R. Rinaldi

University of Salento

Edwin Donath

Leipzig University

Nemany A.N. Hanafy

Kafrelsheikh University

Mariafrancesca Cascione

University of Salento

Helmuth Möhwald

Max Planck Institute of Colloids and Interfaces

Viviana Vergaro

University of Salento

Giuseppe Gigli

Istituto di Nanotecnologia

Gianluigi Giannelli

Gastroenterology Hospital "Saverio de Bellis"

Sergio Moya

CIC biomaGUNE

Concetta Nobile

National Research Council

Cooperative Institutions

University of Salento

National Research Council

Istituto Nanoscienze

University of Bari Aldo Moro

Istituto Nazionale di Fisica Nucleare, Sezione di Lecce

Istituto di Nanotecnologia

Max Planck Institute of Colloids and Interfaces

Innovation Engineering (Italy)

Max Planck Society

Italian Institute of Technology

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Mucoadhesive curcumin crosslinked carboxy methyl cellulose might increase inhibitory efficiency for liver cancer treatment

Materials Science and Engineering C (2020)

Nemany A.N. Hanafy Stefano Leporatti Maged El‐Kemary

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10.1016/j.msec.2020.111119

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Citations (42)

Structure analysis of Eledone cirrhosa by CLSM and SFM

Alberto Diaspro Francesco Beltrame Marco Fato Stefano Leporatti Agostino Palmeri

CLSM (Confocal Laser Scanning Microscopy) and SFM (Scanning Force Microscopy) can be considered in use as high-powered biophysical tools for investigating the very delicate and complex way in which structure and function are related in biological systems. Organisational motifs underlying the structure of important biostructures can be imaged in a hydrated state in living samples or under conditions that are very close to living or physiological states. Among these biostructures, helically organised ones, such as chromatin-DNA, play a fundamental role in the way in which a cell functions. We are studying the structure of the sperm head of the octopus Eledone cirrhosa by means of CSLM and SFM. In particular we aim to study at different resolution levels the sperm head and its constituents and to use the very same sample as a large copy of chromatin itself. Earlier studies concluded that the rigid helical structure of the sperm head is due to its chromatin; probably this is true also for the shape of the head itself as found for several animal species. These studies reported that DNA begins to form 10 nm fibers during spermiogenesis and the fibers condenses further as the process evolves, but no model was obtained to describe the final arrangement of DNA. The possibility of performing such high resolution experiments could give useful hints to solve this problem.

10.1109/iembs.1996.651930

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Resveratrol downregulates Akt/GSK and ERK signalling pathways in OVCAR-3 ovarian cancer cells

Molecular BioSystems (2012)

Daniele Vergara Pasquale Simeone Daniela Toraldo Piero Del Boccio Viviana Vergaro

Phytochemicals constitute a heterogeneous group of substances with an evident role in human health. Their properties on cancer initiation, promotion and progression are well documented. Particular attention is now devoted to better understand the molecular basis of their anticancer action. In the present work, we studied the effect of resveratrol on the ovarian cancer cell line OVCAR-3 by a proteomic approach. Our findings demonstrate that resveratrol down-regulates the protein cyclin D1 and, in a concentration dependent manner, the phosphorylation levels of protein kinase B (Akt) and glycogen synthase kinase-3β (GSK-3β). The dephosphorylation of these kinases could be responsible for the decreased cyclin D1 levels observed after treatment. We also showed that resveratrol reduces phosphorylation levels of the extracellular signal-regulated kinase (ERK) 1/2. Chemical inhibitors of phosphatidylinositol 3-kinase (PI3K) and ERK both increased the in vitro therapeutic efficacy of resveratrol. Moreover, resveratrol had an inhibitory effect on the AKT phosphorylation in cultured cells derived from the ascites of ovarian cancer patients and in a panel of human cancer cell lines. Thus, resveratrol shows antitumor activity in human ovarian cancer cell lines targeting signalling pathway involved in cell proliferation and drug-resistance.

10.1039/c2mb05486h

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Encapsulation of Thermo-Sensitive Lauric Acid in Silica Shell: A Green Derivate for Chemo-Thermal Therapy in Breast Cancer Cell

Molecules (2019)

Valeria De Matteis Mariafrancesca Cascione Maria Luisa De Giorgi Stefano Leporatti R. Rinaldi

Lauric acid is a green derivate that is abundant in some seeds such as coconut oil where it represents the most relevant fatty acid. Some studies have emphasized its anticancer effect due to apoptosis induction. In addition, the lauric acid is a Phase Change Material having a melting temperature of about 43.2 °C: this property makes it a powerful tool in cancer treatment by hyperthermal stress, generally induced at 43 °C. However, the direct use of lauric acid can have some controversial effects, and it can undergo degradation phenomena in the extracellular environment. For this reason, we have encapsulated lauric acid in a silica shell with a one-step and reproducible synthetic route in order to obtain a monodispersed SiO2@LA NPs with a good encapsulation efficiency. We have used these NPs to expose breast cancer cell lines (MCF-7) at different concentrations in combination with hyperthermal treatment. Uptake, viability, oxidative stress induction, caspases levels, and morphometric parameters were analyzed. These nanovectors showed double action in anticancer treatments thanks to the synergic effect of temperature and lauric acid activity.

10.3390/molecules24112034

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Amino-functionalized mesoporous silica nanoparticles (NH2-MSiNPs) impair the embryonic development of the sea urchin Paracentrotus lividus

Environmental Toxicology and Pharmacology (2022)

Stefano Tacconi Simone Augello Francesca Persano Carolina Sbarigia Elisabetta Carata

Paracentrotus lividus

Developmental toxicity

10.1016/j.etap.2022.103956

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Conjugation Of EGCG And Chitosan NPs As A Novel Nano-Drug Delivery System

DOAJ (DOAJ: Directory of Open Access Journals) (2019)

Safer AM Stefano Leporatti J. Ermine Jose M. S. Solimán

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Elasticity and adhesion of resting and lipopolysaccharide‐stimulated macrophages

FEBS Letters (2005)

Stefano Leporatti Anja Gerth Guido Köhler Bernd Kohlstrunk Sunna Hauschildt

Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS‐stimulated cells (separation force = 37 ± 3 nN) was almost twice as high as that of resting macrophages (16 ± 1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young's modulus K , a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.

Elasticity

10.1016/j.febslet.2005.12.037

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Proteomics analysis of E-cadherin knockdown in epithelial breast cancer cells

Journal of Biotechnology (2014)

Daniele Vergara Pasquale Simeone Dominga Latorre Mariafrancesca Cascione Stefano Leporatti

Adherens junction

Proteome

10.1016/j.jbiotec.2014.10.034

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Protamine Assembled in Multilayers on Colloidal Particles Can Be Exchanged and Released

Biomacromolecules (2004)

Sabine Hiller Stefano Leporatti Andreas Schnäckel Elke Typlt Edwin Donath

Biocomposite thin films assembled on colloidal particles by means of layer-by-layer adsorption have been suggested as drug carriers and diagnostic devices. Protamine (PRM)/dextransulfate (DXS) and protamine/bovine serum albumine (BSA) multilayers were fabricated on colloidal silica and subsequently investigated by means of fluorescence activated cell sorting (FACS) and microelectrophoresis. Fluorescein labeled polyelectrolytes were embedded at different positions in the multilayers as a marker for layer growth. FACS showed that PRM and DXS formed regular growing stable multilayers, yet adsorbed PRM can be nevertheless exchanged with PRM in solution during layer formation and also after the multilayer formation has been completed. Up to 90% of the PRM pool was available for exchange. PRM together with BSA as demonstrated by SFM did not form multilayers under the applied conditions although the zeta-potential, commonly used as an indicator for stepwise adsorption, observed characteristic alternations. The capability of bound PRM to exchange with PRM in solution is attributed to its relatively small size. The demonstrated exchange may have importance in designing multilayers with smart release features. Furthermore, FACS proved to be a rather suitable means to quantify the aggregation behavior during coating and washing. Singulets, doublets, triplets, and aggregates of higher order could be clearly resolved. The aggregation of particles coated with PRM/DXS layers was higher than that of silica particles coated with PAH/PSS layers. In the first case about 50% of all recorded events are attributed to aggregats, while the PAH/PSS coating produced only about 10% aggregates.

Bovine serum albumin

Layer by layer

Microelectrophoresis

10.1021/bm049875m

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Citations (34)

Cytocompatibility and Uptake of Halloysite Clay Nanotubes

Biomacromolecules (2010)

Viviana Vergaro Elshad Abdullayev Yuri Lvov Andre Zeitoun R. Cingolani

Halloysite is aluminosilicate clay with hollow tubular structure of 50 nm external diameter and 15 nm diameter lumen. Halloysite biocompatibility study is important for its potential applications in polymer composites, bone implants, controlled drug delivery, and for protective coating (e.g., anticorrosion or antimolding). Halloysite nanotubes were added to different cell cultures for toxicity tests. Its fluorescence functionalization by aminopropyltriethosilane (APTES) and with fluorescently labeled polyelectrolyte layers allowed following halloysite uptake by the cells with confocal laser scanning microscopy (CLSM). Quantitative Trypan blue and MTT measurements performed with two neoplastic cell lines model systems as a function of the nanotubes concentration and incubation time indicate that halloysite exhibits a high level of biocompatibility and very low cytotoxicity, rendering it a good candidate for household materials and medicine. A combination of transmission electron microscopy (TEM), scanning electron microscopy (SEM), and scanning force microscopy (SFM) imaging techniques have been employed to elucidate the structure of halloysite nanotubes.

Halloysite

Biocompatibility

Trypan blue

10.1021/bm9014446

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Citations (605)