To detect changes of Foxp3 expression in the decidua in patients with preeclampsia and investigate the correlation of Foxp3-924 (rs2232365) polymorphisms with preeclampsia.From October 2011 to December 2012, 252 normal pregnant women and 156 preeclampsia patients of Han nationality from the same geographic region were tested for Foxp3-924 genotypes by polymerase chain reaction with sequence-specific primer (PCR-SSP). Sixty-eight of the patients with preeclampsia (33 with mild and 35 with severe preeclampsia) and 30 of the normal pregnant women were also examined for Foxp3 expression in the decidua using immunohistochemical method.Foxp3 positive expression rates in the decidua was 51.52% in mild preeclampsia and 28.57% in severe preeclampsia cases, significantly lower than that in the control group (86.67%, P<0.05). In preeclampsia patients, the frequencies of Foxp3-924G/G, G/A, and A/A genotypes were 0.1346, 0.4615 and 0.4038, respectively, and the frequencies of Foxp3-924A and Foxp3-924 G were 0.6346 and 0.3654, respectively. The genotype frequencies of Foxp3-924G/G, G/A and A/A in the control group were 0.1508, 0.4087 and 0.4405, respectively, and the frequencies of Foxp3-924 A and Foxp3-924 G were 0.6448 and 0.3552, respectively. No significant differences were found in the gene frequencies of Foxp3-924G/A between preeclampsia patients and the control group (P>0.05).The expression level of Foxp3 in the placental tissue of preeclampsia patients is significantly lower than that in normal pregnant women, suggesting that lowered Foxp3 expression decreases the immunosuppressive function and causes imbalance of immune tolerance between maternal-fetal to induce preeclampsia. Foxp3-924 polymorphisms is not significantly correlated with the occurrence of preeclampsia.
To evaluate the potential influence of Foxp3 polymorphism on preeclampsia (PE) susceptibility, we conducted a case-control study in Han Chinese women.Foxp3 genotyping was determined by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 156 PE patients and 252 age-frequency matched controls. Immunohistochemical staining was used to detect the expression of Foxp3 specific transcription factor in 30 PE and 30 normal pregnant women.The positive rate of Foxp3 expression in PE (26.67%) was significant difference from that in normal control (63.33%, P<0.05). The frequency of Foxp3-6054 TT genotype was significantly lower in PE patient than that in control. No significant difference was found in Foxp3-3279 genotypes between PE and control, as well as for the variant allele. The frequency of Foxp3-6054A/-3279C haplotype in PE was significantly higher than that in control (P<0.01), while the frequency of Foxp3 6054T/-3279C haplotype was significantly lower in PE patient than that in control (P<0.01).Our findings suggest that the immune suppression function in PE patients is weakened, which may result in the occurrence of PE. Foxp3 polymorphism (rs5902434) may be a potential contributor for the development of PE in Han Chinese women.
Institutional affiliation 1 for authors Ximing Chen and Jianhua Xiao is incorrect. The correct affiliation is: 1. Medical College of University of South China， Hengyang, China.
In addition, the Corresponding Author for the article was incorrectly listed as Ximing Chen. The Corresponding Author is Jianhua Xiao, who can be reached at JianhuaXia2010@163.com
Objective:To study on the characteristics of early onset severe pre-eclampsia by comparing the data observed at different gestational weeks.Methods:One hundred and forty-six cases meeting the criteria of severe pre-eclampsia were enrolled in this study.Patients were divided into 2 groups: early onset group(n=20) with those onset ≤ 34 weeks of gestation and late onset group(n=126)with onset34weeks of gestation.The basic data of gravidas,child bearing history,medical examination results,and outcome of the perinatal were compared of the two groups.Results:There were less gravida in early onset group than in late onset group who had standard antenatal care.Percentages of primipara,and those who had discomfort were different between the two group(P0.05).The incidence of complication and FGR,low birth weight infant and perinatal mortality were also different between the two group.The male-to-female ratio of babies was 0.82 and 0.97 respectively in the two groups.There were no obviously difference of the ratios of the primigravida between the two groups.Whether be a primipara was no associated with the early onset severe pre-eclampsia.Conclusion:Onset of severe pre-eclampsia different gestational week may have difference outcomes.The early onset severe pre-eclampsia may be regarded as a special type of severe pre-eclampsia.
Objective To investigate the correlation between A66G polymorphism of methionine synthase reductase (MTRR) and the incidence of pregnancy induced hypertension syndrome. Methods We analyzed the A66G polymorphism of MTRR in pregnancy women of normal and PIH by means of PCR and RFLP. Results There were no siganificant differences in the frequenceies of methionine synthase reductase mutations between the patients and central groups , The level of tHcy in pregnancy women of PIH was much more than that in pregnancy women of normal group. Conclusions The point mutation of A66G of MTRR may have no relationship with PIH.
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