Caroline Brito da Silva

Universidade Federal de Viçosa

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Gustavo de Amorim Rodrigues

Universidade Federal de Viçosa

Marcos Henrique Soares

Universidade Federal de Viçosa

Alysson Saraiva

Universidade Federal de Viçosa

Dante Teixeira Valente Júnior

Universidade Federal de Viçosa

Lívia Maria dos Reis Barbosa

Universidade Federal de Viçosa

Gabriel Cipriano Rocha

North Carolina State University

Arele Arlindo Calderano

Universidade Federal de Viçosa

Hilário Cuquetto Mantovani

University of Wisconsin–Madison

Márcio de Souza Duarte

University of Guelph

Graziela Alves da Cunha Valini

Universidade Estadual Paulista (Unesp)

Cooperative Institutions

Universidade Federal de Viçosa

ORCID

Institute of Animal Science and Pastures

Institute of Animal Sciences

North Carolina State University

Universidade Federal de Lavras

University of Guelph

Universidade Federal dos Vales do Jequitinhonha e Mucuri

Instituto Federal Fluminense

Universidade Estadual do Oeste do Paraná

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Probióticos como alternativa ao óxido de zinco em dietas de leitões dos 21 aos 35 dias de idade

Caroline Brito da Silva

O desmame é um dos períodos mais críticos na produção de suínos, caracterizado por mudanças ambientais, nutricionais e sociais que podem resultar em disbiose intestinal e aumento da incidência de diarreia. Uma abordagem para mitigar esses problemas tem sido o uso de doses farmacológicas de óxido de zinco (ZnO), que ajudam a controlar a diarreia pós- desmame. No entanto, o uso de ZnO em níveis elevados tem sido associado a preocupações ambientais e ao desenvolvimento de resistência antimicrobiana. Em 2022, a União Europeia baniu o uso de ZnO em doses farmacológicas, o que intensificou a busca por alternativas eficazes. Diante da proibição do ZnO, os probióticos emergem como uma alternativa promissora no controle da diarreia em leitões desmamados. Este estudo foi realizado com o objetivo de avaliar os efeitos dos probióticos Bacillus subtilis (GutPlus ®) e Bacillus amyloliquefaciens (Ecobiol®) no desempenho, morfologia intestinal e expressão de genes associados à integridade da barreira intestinal e ativação do sistema imunológico em leitões desmamados aos 21 dias de idade. Foram utilizados 150 leitões, desmamados aos 21 dias de idade, com peso inicial de 6,78 ± 0,88 kg, distribuídos em delineamento experimental em blocos (peso inicial) casualizados, em 5 dietas, 10 repetições e 3 animais por unidade experimental. As dietas foram constituídas por uma ração a base de milho e farelo de soja, sem adição de aditivos (controle negativo, CN); CN + 2500 mg/kg de ZnO (controle positivo, CP); CN + 500 g/Ton de GutPlus ® (SUB); CN + 1000 g/Ton de Ecobiol ® (AMY); CN + 250 g/Ton de GutPlus® + 500 g/Ton de Ecobiol ® (SUB+AMY). Não foram observados efeitos (P > 0,05) entre as dietas para consumo de ração diário, ganho de peso diário e conversão alimentar. Entretanto, os leitões que receberam a dieta CP apresentaram maior peso final (P < 0,05). As dietas CN e CP resultaram nos maiores (P < 0,05) valores de escore fecal, enquanto os grupos SUB, AMY e SUB+AMY apresentaram valores intermediários, sem diferença entre eles. Em relação à incidência de diarreia, CN teve o maior percentual, enquanto CP apresentou a menor incidência. As dietas SUB, AMY e SUB+AMY resultaram em valores intermediários para a incidência de diarreia. No duodeno, não foram observadas diferenças (P > 0,05) entre as dietas em relação à altura das vilosidades (AV), profundidade das criptas (PC) ou relação vilo: cripta (AV:PC). No entanto, no jejuno, os leitões alimentados com as dietas CP e AMY apresentaram valores maiores de AV:PC (P < 0,05) em comparação aos leitões do grupo CN. No íleo, a altura das vilosidades e a profundidade das criptas não diferiram entre os grupos (P > 0,05), porém, a relação AV:PC foi maior (P < 0,05) nos leitões alimentados com as dietas CP e SUB+AMY em comparação aos que receberam a dieta CN. Não foram observadas diferenças (P > 0,05) entre as dietas para a expressão dos genes HAP, IL-1β, IL-6, TGF-β, TNF e OCL no jejuno dos leitões. No entanto, foi verificado aumento (P < 0,05) na expressão do gene IL-10 nas dietas CP e AMY em comparação com o grupo CN. Não houve diferença (P > 0,05) na expressão dos genes ocludina e zonula occludens, relacionados à integridade da barreira intestinal. A suplementação com Bacillus amyloliquefaciens, isoladamente ou em combinação com Bacillus subtilis, melhora a relação vilo: cripta no jejuno e íleo e reduz a incidência de diarreia em leitões desmamados. Além disso, esses probióticos aumentam a expressão do gene anti-inflamatório IL-10, evidenciando seu potencial para melhorar a saúde intestinal. Palavras-chave: Suplementação. Desmame. Nutrição. Saúde intestinal.

10.47328/ufvbbt.2024.595

Cite

Citations (0)

Suplementação de cromo na dieta de suínos em terminação

Ciência Rural (2021)

Dante Teixeira Valente Júnior Lívia Maria dos Reis Barbosa Marcos Henrique Soares Gustavo de Amorim Rodrigues Maykelly da Silva Gomes

EnglishIncreasing fat deposition and feed conversion ratio over the days in finishing phase directly influence pork quality and productive profitability. Nonetheless, the slaughter of heavier pigs can result in benefits for the slaughterhouse due to dilution of production and processing costs, as well as economic benefits for the pig producer resulting from the dilution of production costs. Therefore, dietary supplementation of chromium for finishing pigs of high lean-genotypes is a strategy to increase lean tissue accretion and minimize fat deposition, reflecting positively on growth performance. This review discussed recent studies results and mechanisms of action of this modifier of performance and carcass a traits in finishing pigs. Chromium increases the insulin action, facilitating insulin binding to the receptors on cell membrane. As a result, insulin-sensitive cells uptake more glucose, which will be later converted into energy. This additional energy is use to increase protein synthesis, thereby increasing the amount of lean tissue and reducing fat content in the carcass, without altering protein intake by pigs. Chromium also reduces lipid oxidation rate maintaining meat quality for longer period. In conclusion, dietary Cr supplementation for finishing pigs have shown that 0.2 mg/kg of organic Cr sources for pigs from ~60 kg until the slaughter can improve growth performance, lean gain and reduce fat content in carcass. However, the development of nanotechnology has allowed the use of inorganic Cr source at 0.2 mg/kg of inclusion, leading to improve the growth performance and carcass traits of finishing pigs portuguesO aumento na deposicao de gordura e da conversao alimentar durante a fase de terminacao sao fatores que implicam diretamente na qualidade da carne e tambem na rentabilidade da cadeia produtiva. Apesar disso, o abate tardio dos suinos pode resultar em beneficios para o frigorifico, pela reducao dos custos operacionais por suino abatido e melhor utilizacao dos equipamentos, bem como vantagens economicas para o produtor resultantes da diluicao dos custos de producao. Portanto, a suplementacao de cromo na racao de suinos de linhagens com maior potencial para deposicao de musculo, e uma estrategia para aumentar a deposicao de tecido magro e minimizar a deposicao de gordura, refletindo positivamente no desempenho. Em vista disso, objetivou-se com este trabalho, apresentar e discutir os mecanismos de acao, finalidades e resultados recentes de estudos na literatura sobre a suplementacao desse modificador de carcaca na racao de suinos em terminacao. O principal papel do cromo e potencializar a acao da insulina, facilitando a ligacao entre a insulina e os seus receptores na membrana celular. Com isso, as celulas sensiveis a insulina captam maior quantidade de glicose, que posteriormente serao convertidas em energia. Essa energia adicional pode ser utilizada para aumentar a sintese proteica, aumentando assim, a quantidade de carne magra e reduzindo o teor de gordura na carcaca, sem alterar o consumo de proteina pelos animais. O cromo tambem diminui a taxa de oxidacao lipidica, mantendo a qualidade da carne por mais tempo. Em conclusao, a suplementacao dietetica de Cr para suinos em terminacao mostra que 0,2 mg/kg de fontes orgânicas de Cr para suinos de ~ 60 kg ate o abate podem melhorar o desempenho, ganho de massa magra e reduzir o teor de gordura na carcaca. No entanto, o desenvolvimento da nanotecnologia tem permitido o uso de fonte inorgânica de Cr na dose de 0,2 mg/kg de inclusao, melhorando o desempenho e as caracteristicas de carcaca de suinos em terminacao

Lean meat

Source

Cite

Citations (0)

Dietary supplementation of chromium for finishing pigs

Ciência Rural (2021)

Dante Teixeira Valente Júnior Lívia Maria dos Reis Barbosa Marcos Henrique Soares Gustavo de Amorim Rodrigues Maykelly da Silva Gomes

ABSTRACT: Increasing fat deposition and feed conversion ratio over the days in finishing phase directly influence pork quality and productive profitability. Nonetheless, the slaughter of heavier pigs can result in benefits for the slaughterhouse due to dilution of production and processing costs, as well as economic benefits for the pig producer resulting from the dilution of production costs. Therefore, dietary supplementation of chromium for finishing pigs of high lean-genotypes is a strategy to increase lean tissue accretion and minimize fat deposition, reflecting positively on growth performance. This review discussed recent studies results and mechanisms of action of this modifier of performance and carcass a traits in finishing pigs. Chromium increases the insulin action, facilitating insulin binding to the receptors on cell membrane. As a result, insulin-sensitive cells uptake more glucose, which will be later converted into energy. This additional energy is use to increase protein synthesis, thereby increasing the amount of lean tissue and reducing fat content in the carcass, without altering protein intake by pigs. Chromium also reduces lipid oxidation rate maintaining meat quality for longer period. In conclusion, dietary Cr supplementation for finishing pigs have shown that 0.2 mg/kg of organic Cr sources for pigs from ~60 kg until the slaughter can improve growth performance, lean gain and reduce fat content in carcass. However, the development of nanotechnology has allowed the use of inorganic Cr source at 0.2 mg/kg of inclusion, leading to improve the growth performance and carcass traits of finishing pigs.

10.1590/0103-8478cr20200554

Cite

Citations (5)

Performance, Carcass Traits, Pork Quality and Expression of Genes Related to Intramuscular Fat Metabolism of Two Diverse Genetic Lines of Pigs

Foods (2022)

Marcos Henrique Soares Gustavo de Amorim Rodrigues Dante Teixeira Valente Júnior Caroline Brito da Silva Thaís Correia Costa

We aimed to evaluate the performance, carcass and pork quality traits, as well as the mRNA expression of genes related to intramuscular fat deposition in female pigs from different genetic lines. A total of eighteen female pigs (Large White × Landrace × Duroc × Pietrain) × (Large White × Landrace) (Hybrid) averaging 88.96 ± 3.44 kg in body weight and twelve female pigs (Duroc) × (Large White × Landrace) (Duroc) averaging 85.63 ± 1.55 kg in body weight were assigned to a completely randomized design experimental trial that lasted 45 days. Pigs from both genetic lines received the same diet, which was initially adjusted for their nutritional requirements from 0 to 17 days of age and subsequently adjusted for nutritional requirements from 17 to 45 days of age. The performance of pigs did not differ among groups (p > 0.05). Duroc pigs showed a lower backfat thickness (p < 0.03) and greater intramuscular fat content (p < 0.1). A greater mRNA expression of the peroxisome proliferator-activated receptor gamma gene (PPARγ, p = 0.008) and fatty acid protein translocase/cluster differentiation (FAT/CD36, p = 0.002) was observed in the Longissimus dorsi muscle of Duroc pigs. Similarly, a greater expression of PPARγ (p = 0.009) and FAT/CD36 (p = 0.02) was observed in the Soleus muscle of Duroc pigs. Overall, we observed that despite the lack of differences in performance between the genetic groups, Duroc pigs had greater intramuscular fat content than hybrid pigs. The increased intramuscular fat content was associated with an increase in the mRNA expression of key transcriptional factors and genes encoding enzymes involved in adipogenesis and lipogenesis in glycolytic and oxidative skeletal muscle tissues.

Intramuscular fat

Large white

CD36

Intramuscular injection

10.3390/foods11152280

Cite

Citations (5)

L-Arginine Supplementation for Nulliparous Sows during the Last Third of Gestation

Animals (2021)

Gustavo de Amorim Rodrigues Dante Teixeira Valente Júnior Marcos Henrique Soares Caroline Brito da Silva Fernanda Abranches Fialho

We evaluated the effects of L-arginine supplementation during the last third of gestation on molecular mechanisms related to skeletal muscle development of piglets and litter traits at birth. Twenty-three nulliparous sows averaging 205.37 ± 11.50 kg of body weight were randomly assigned to the following experimental treatments: control (CON), where pregnant sows were fed diets to meet their nutritional requirements; arginine (ARG), where sows where fed CON + 1.0% L-arginine. Skeletal muscle from piglets born from sows from ARG group had greater mRNA expression of MYOD (p = 0.043) and MYOG (p ≤ 0.01), and tended to present greater mRNA expression (p = 0.06) of IGF-2 gene compared to those born from CON sows. However, there were no differences (p > 0.05) in the histomorphometric variables of fetuses' skeletal muscle. The total weight of born piglets, total weight of born alive piglets, piglet weight at birth, coefficient of variation of birth weight, and the incidence of intrauterine growth restriction (IUGR) piglets did not differ between groups. No stillborn piglets (p < 0.01) were verified in the ARG sows compared to CON group. The blood levels of estradiol (p = 0.035) and urea (p = 0.03) were higher in ARG sows compared to those from the CON group. In summary, our data show that arginine supplementation of nulliparous sows at late gestation enhance mRNA expression of key myogenic regulatory factors, which likely contribute to improve animal growth rates in later stages of development.

Litter

Intrauterine growth restriction

10.3390/ani11123476

Cite

Citations (4)

Effects of corn processing on piglet performance and intestinal parameters

Figshare (2021)

Tiago Scalco Graziela Alves da Cunha Valini Caroline Brito da Silva Arele Arlindo Calderano Gustavo de Amorim Rodrigues

ABSTRACT The objective of the present study was to compare the effects of corn processing on performance and intestinal parameters of weanling piglets. To accomplish our goal, 42 piglets (21 days-old, 7.18±1.0 kg body weight) were randomly allocated (seven pens/treatment; three pigs/pen) to one of two treatments: ground corn – corn ground through a hammer mill with a 1.0-mm screen after being ground by a hammer mill with a 3.0-mm screen, and extruded corn – corn was wet extruded after being ground by a hammer mill with a 2.0-mm screen and, after extrusion, it was ground by a hammer mill with a 1.0-mm screen. In both methods, corn from the same batch was used. Results were considered statistically significant when P<0.05 and a tendency when P<0.10. Analyses were performed using the GLIMMIX procedure of SAS. Pigs fed ground or extruded corn diet had similar growth performance. Duodenum and jejunum of pigs fed extruded corn had greater villus height compared with those of pigs fed ground corn. Crypt depth was not influenced by corn processing. Duodenum and jejunum of pigs fed extruded corn had greater villus:crypt ratio compared with those of pigs fed ground corn. In the duodenum, the expression of zonula occludens-1 (ZO-1) was greater, and the expression of Occludin-1 tended to be greater in pigs fed the extruded corn diets. There was no effect of corn processing on ZO-1 and Occludin-1 expression in the jejunum, nor TGF-β1 expression in duodenum and jejunum. There was no effect of the type of corn processing on colonic total bacteria or Enterobacteriaceae and Lactobacillus abundance. Piglets fed diets with ground corn or extruded corn have similar growth performance. However, piglets fed diets with extruded corn present improved intestinal morphology and tight junction protein expression compared with those fed ground corn.

10.6084/m9.figshare.14306485

Cite

Citations (0)

Effects of corn processing on piglet performance and intestinal parameters

Revista Brasileira de Zootecnia (2020)

Tiago Scalco Graziela Alves da Cunha Valini Caroline Brito da Silva Arele Arlindo Calderano Gustavo de Amorim Rodrigues

The objective of the present study was to compare the effects of corn processing on performance and intestinal parameters of weanling piglets. To accomplish our goal, 42 piglets (21 days-old, 7.18±1.0 kg body weight) were randomly allocated (seven pens/treatment; three pigs/pen) to one of two treatments: ground corn – corn ground through a hammer mill with a 1.0-mm screen after being ground by a hammer mill with a 3.0-mm screen, and extruded corn – corn was wet extruded after [...]

Weanling

Corn oil

Hammer

10.37496/rbz4920200133

Cite

Citations (0)