Toshio Fukao

Gifu Municipal Hospital

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Chie Monma

Tokyo Metropolitan Institute of Public Health

Masakado Matsumoto

Aichi Prefectural Government

Miwako Shimizu

Maebashi Institute of Technology

Junko Isobe

Toyama Institute of Health

Takayoshi Yamagishi

Kanazawa University

Kazukiyo Yamaoka

Gunma Prefectural Institute of Public Health and Environmental Sciences

Keiko Kimata

Toyama Institute of Health

Reiji Hiramatsu

Aichi Prefectural Government

Makiko Yamada

Memorial Sloan Kettering Cancer Center

Tadahiro Karasawa

Kanazawa University

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Kanazawa University

Toyama Institute of Health

Osaka Prefectural Institute of Public Health

Nagoya University

National Institute of Infectious Diseases

Gifu Pharmaceutical University

Osaka University

Gunma Prefectural Institute of Public Health and Environmental Sciences

Aichi Prefectural Government

Aichi Cancer Center

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New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates.

Biological and Pharmaceutical Bulletin (2002)

Saori Itoh Masako Kariya Keiji Nagano Shin‐ichiro Yokoyama Toshio Fukao

An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22microm membrane (MultiScreen-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.

Filtration (mathematics)

Antibody titer

10.1248/bpb.25.986

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Citations (30)

Genotyping of Clostridium perfringens isolates collected from food poisoning outbreaks and healthy individuals in Japan based on the cpe locus.

PubMed (2007)

Daisuke Tanaka Keiko Kimata Miwako Shimizu Junko Isobe Masanori Watahiki

Daisuke Tanaka*, Keiko Kimata, Miwako Shimizu, Junko Isobe, Masanori Watahiki, Tadahiro Karasawa, Takayoshi Yamagishi, Sanae Kuramoto, Toshihiko Serikawa, Fubito Ishiguro, Makiko Yamada, Kazukiyo Yamaoka, Mitsuo Tokoro, Toshio Fukao, Masakado Matsumoto, Reiji Hiramatsu, Chie Monma and Yoshiyuki Nagai Toyama Institute of Health, Toyama 939-0363; Department of Clinical Laboratory Science, Kanazawa University Graduate School of Medical Science, Ishikawa 920-0942; Ishikawa Prefectural Institute of Public Health and Environmental Science, Ishikawa 920-1154; Fukui Prefectural Institute of Public Health and Environmental Science, Fukui 910-8551; Gifu Prefectural Institute of Health and Environmental Sciences, Gifu 504-0838; Gifu Municipal Institute of Public Health, Gifu 500-8881; Aichi Prefectural Institute of Public Health, Aichi 462-8576; and Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169-0073, Japan

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Citations (30)

Genotyping of <i>Clostridium perfringens</i> Isolates Collected from Food Poisoning Outbreaks and Healthy Individuals in Japan Based on the <i>cpe</i> Locus

Japanese Journal of Infectious Diseases (2007)

Daisuke Tanaka Keiko Kimata Miwako Shimizu Junko Isobe Masanori Watahiki

Clostridium perfringens

Food poisoning

10.7883/yoken.jjid.2007.68

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Citations (26)

An Outbreak of Diarrheal Disease Caused by Enterotoxigenic Clostridium Perfringens Following Exposure to a Contaminated Enviroment in a Nursing Home

Kansenshogaku zasshi (2004)

Toshio Fukao Megumi Sato Yasunori Tanaka Chie Monma Naoki Kato

We herein report an outbreak of non-food-borne diarrhea which occurred in a nursing home due to enterotoxigenic Clostridium perfringens. The regional public health center in Gifu, Japan, recognized 7 patients with diarrhea in a nursing home, suspecting a food-borne illness. Bacteriological and epidemiological studies indicated that enterotoxigenic C. perfringens was the causative agent. However, suspected foods, the kitchen and the cooks carried no enteropathogenic bacteria, indicating that this outbreak was a non-food-borne diarrhea. The swab specimens obtained from the residential area of the nursing home were found to have enterotoxigenic C. perfringens. Isolates from the stool specimens of patients and environment were all serotype TW47, showing susceptibilities to ampicillin, levofloxacin, and clindamycin very similar to each other, and had banding patterns identical to each other by pulsed-field gel electrophoresis. These resultstrongly supported the existence of monoclonal spread of an enterotoxigenic C. perfringens among the environment of the nursing home and the residents. During 3 weeks 14 residents were involved in this outbreak. The extensive effort of keeping the residential area clean led to a prompt cease of this outbreak.

Clostridium perfringens

Enterotoxigenic Escherichia coli

Food poisoning

10.11150/kansenshogakuzasshi1970.78.32

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Citations (5)