María L. Ramirez

National University of Río Cuarto

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S. Chulze

National University of Río Cuarto

Adriana M. Torres

National University of Río Cuarto

Eugenia Cendoya

Instituto Médico Río Cuarto

M.M. Reynoso

Consejo Nacional de Investigaciones Científicas y Técnicas

María Cecilia Farnochi

National University of Río Cuarto

María J. Nichea

Instituto Médico Río Cuarto

Celia Renata Rosemberg

University of Buenos Aires

Vanessa G. L. Zachetti

Consejo Nacional de Investigaciones Científicas y Técnicas

Sofía A. Palacios

Consejo Nacional de Investigaciones Científicas y Técnicas

Germán Barros

Consejo Nacional de Investigaciones Científicas y Técnicas

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

National University of Río Cuarto

Centro Científico Tecnológico - San Juan

Centro Científico Tecnológico - Tucumán

University of Buenos Aires

Agriculture and Food

University of Queensland

National Agricultural Technology Institute

Universidad Nacional de Asunción

Instituto Médico Río Cuarto

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Combination of Bacillus velezensis RC218 and Chitosan to Control Fusarium Head Blight on Bread and Durum Wheat under Greenhouse and Field Conditions

Toxins (2022)

Juan Manuel Palazzini Agustina Reynoso Nadia Yerkovich Vanessa G. L. Zachetti María L. Ramirez

Fusarium graminearum sensu stricto is, worldwide, the main causal agent of Fusarium head blight in small cereal crops such as wheat, barley, and oat. The pathogen causes not only reductions in yield and grain quality but also contamination with type-B trichothecenes such as deoxynivalenol. Prevention strategies include the use of less susceptible cultivars through breeding programs, cultural practices, crop rotation, fungicide application, or a combination of them through an integrated pest management. Additionally, the use of more eco-friendly strategies by the evaluation of microorganisms and natural products is increasing. The effect of combining Bacillus velezensis RC218 and chitosan on Fusarium Head Blight (FHB) and deoxynivalenol accumulation under greenhouse and field conditions in bread and durum wheat was evaluated. Under greenhouse conditions, both B. velezensis RC218 and chitosan (0.1%) demonstrated FHB control, diminishing the severity by 38 and 27%, respectively, while the combined treatment resulted in an increased reduction of 54% on bread wheat. Field trials on bread wheat showed a biocontrol reduction in FHB by 18 to 53%, and chitosan was effective only during the first year (48% reduction); surprisingly, the combination of these active principles allowed the control of FHB disease severity by 39 and 36.7% during the two harvest seasons evaluated (2017/18, 2018/19). On durum wheat, the combined treatment showed a 54.3% disease severity reduction. A reduction in DON accumulation in harvested grains was observed for either bacteria, chitosan, or their combination, with reductions of 50.3, 68, and 64.5%, respectively, versus the control.

Crop Rotation

10.3390/toxins14070499

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Citations (15)

Impact of environmental factors and fungicides on growth and deoxinivalenol production by Fusarium graminearum isolates from Argentinian wheat

Crop Protection (2003)

María L. Ramirez S. Chulze Naresh Magan

Tebuconazole

Growth inhibition

10.1016/j.cropro.2003.07.005

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Citations (103)

AIMS Genetics (2016)

María Cecilia Rodríguez Marcelo H. Cassini Gabriela Delgado Argentinian Integron Study Group

Class 1 and 2 integrons are considered the paradigm of multidrug resistant (MDR) integrons.Although class 1 integrons have been found statistically associated to Enterobacteriaceae MDR isolates, this type of study has not been conducted for class 2 integrons.Escherichia coli and 3 species that were found that harbored more than 20% of class 2 integrons in clinical isolates, were selected to determine the role of intI2 as MDR marker.A total of 234 MDR/191 susceptible non-epidemiologically related isolates were analyzed.Seventy-four intI2 genes were found by PCR and sequencing.An intI2 relationship with MDR phenotypes in Acinetobacter baumannii and Enterobacter cloacae was found.No statistical association was identified with MDR E. coli and Helicobacter pylori isolates.In other words, the likelihood of finding intI2 is the same in susceptible and in MDR E. coli and H. pylori strains, suggesting a particular affinity between the mobile element Tn7 and some species.The use of intI2 as MDR marker was species-dependent, with fluctuating epidemiology at geographical and temporal gradients.The use of intI2 as MDR marker is advisable in A. baumannii, a species that can reach high frequencies of this genetic element.

10.1055/s-011-50726

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Argumentar en el jardín de infantes: Análisis exploratorio según la actividad y la función pragmática

Traslaciones Revista latinoamericana de Lectura y Escritura (2020)

Maia Julieta Migdalek Nadia Soledad Peralta María L. Ramirez María Ileana Ibañez

El presente trabajo se propone realizar un análisis exploratorio de la producción argumentativa entre niños y docentes de preescolar considerando las actividades en las que ocurre (el juego en rincones y la ronda de intercambio) y las funciones pragmáticas de las emisiones que promueven el despliegue argumentativo. Se analizaron 10 situaciones de juego y 10 de ronda audiograbadas en salas de 5 años de 10 jardines de infantes de Entre Ríos, Argentina. Se identificaron las secuencias conversacionales de Oposición, Argumentación y Justificación (N=116) y se codificó la Identidad de los participantes, el rol argumentativo que estos asumían en las distintas secuencias y las funciones pragmáticas de las emisiones que desencadenaban la presencia del argumento o justificación. Se realizó un análisis de correspondencias múltiples que arrojó tres agrupamientos: el primero vincula la Justificación con las funciones pragmáticas Directiva y Reporte, con un fuerte peso de la maestra en el despliegue de la misma; el segundo agrupa la argumentación con las funciones pragmáticas Pregunta y Comentario y al niño en rol de Oponente y presentando los argumentos; y finalmente, en el tercer agrupamiento, la Simple oposición con el niño como Proponente. Asimismo, el análisis estadístico se combinó con un análisis cualitativo que ejemplifica los agrupamientos identificados. Estos resultados ponen de manifiesto que tanto el juego y como la ronda son actividades igualmente propicias para el despliegue de argumentos y justificaciones.

10.48162/rev.5.009

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Citations (2)

Influence of water activity and temperature on growth and mycotoxin production by Alternaria alternata on irradiated soya beans

International Journal of Food Microbiology (2011)

María Silvina Oviedo María L. Ramirez Germán Barros S. Chulze

Alternariol

Water activity

Moho

10.1016/j.ijfoodmicro.2011.06.007

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Citations (36)

Evaluation of ability of ferulic acid to control growth and fumonisin production of Fusarium verticillioides and Fusarium proliferatum on maize based media

International Journal of Food Microbiology (2013)

Laura V Ferrochio Eugenia Cendoya María Cecilia Farnochi Walter A. Massad María L. Ramirez

Fusarium proliferatum

Fumonisin

10.1016/j.ijfoodmicro.2013.09.005

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Citations (52)

Toxigenic profile of Alternaria alternata and Alternaria infectoria occurring on wheat

María Silvina Oviedo María Elena Sturm M.M. Reynoso S. Chulze María L. Ramirez

Fil: Oviedo, Maria Silvina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;

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Long-term safety, tolerability, and efficacy of extended release somatostatin analogues

Endocrine Abstracts (2015)

Javier Martínez Rogelio García-Centeno Maria Arnoriaga María Picallo María L. Ramirez

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Tolerability

10.1530/endoabs.37.ep678

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Primer registro del ácaro Mononychellus planki (Prostigmata:Tetranychidae) asociado al cultivo de soja en Paraguay

DOAJ (DOAJ: Directory of Open Access Journals) (2018)

María L. Ramirez Osmar Arias Víctor Gómez Gilberto J. de Morães

Prostigmata

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Trichothecene Genotype Profiling of Wheat Fusarium graminearum Species Complex in Paraguay

Toxins (2022)

Andrea Alejandra Arrúa Alvarenga Julio César Masaru Iehisa Ouchi Cinthia Cazal Juliana Moura Mendes Adans A. Colmán

Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.

Chemotype

Sensu stricto

10.3390/toxins14040257

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Citations (6)