Pulicat Manogaran

King Faisal Specialist Hospital & Research Centre

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Shahab Uddin

Qatar University

Khawla S. Al‐Kuraya

King Faisal Specialist Hospital & Research Centre

Leonidas C. Platanias

Northwestern University

Naif A. Al-Jomah

Center for Children

Jehad Abubaker

Dasman Diabetes Institute

Azhar R. Hussain

Aga Khan University

Khalid Al-Hussein

King Faisal Specialist Hospital & Research Centre

Abdul K. Siraj

King Faisal Specialist Hospital & Research Centre

Maqbool Ahmed

Jinnah Postgraduate Medical Center

Asma S. Khan

University of Health Sciences Lahore

Cooperative Institutions

King Faisal Specialist Hospital & Research Centre

Alfaisal University

King Saud University

Centre for Cellular and Molecular Biology

King Fahd Security College

King Saud bin Abdulaziz University for Health Sciences

National Guard Health Affairs

King Abdullah International Medical Research Center

University of Fukui

Riyadh Elm University

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Supplementary Figure 2 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Azhar R. Hussain Naif A. Al-Jomah Abdul K. Siraj Pulicat Manogaran Khalid Al-Hussein

Supplementary Figure 2 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Sanguinarine

Primary effusion lymphoma

10.1158/0008-5472.22370177

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CCDC 992702: Experimental Crystal Structure Determination

The Cambridge Structural Database (2014)

M. A. Bhat Abdullah Al‐Dhfyan Azmat Ali Khan Nouf Alharbi Pulicat Manogaran

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/cc129zn0

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Complete Loss or Severely Compromised Expression of the Pro-Apoptotic Protein Bax Leads to Resistance to Curcumin-Induced Apoptosis in Burkitt’s Lymphoma Cells.

Blood (2005)

Azhar R. Hussain Maha Al‐Rasheed Pulicat Manogaran Khawla S. Al‐Kuraya Shahab Uddin

Primary effusion lymphoma

Viability assay

Burkitt's lymphoma

Bcl-2-associated X protein

10.1182/blood.v106.11.1928.1928

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Citations (1)

Flow cytometric study of changes in the intracellular free calcium during the cell cycle

Cytometry (1996)

Gopal Pande Nirbhay Kumar Pulicat Manogaran

We measured the intracellular levels of free cytoplasmic calcium in different phases of the cell cycle in viable rat fibroblasts, using two parameter flow cytometric analysis with Hoechst 33342 as the DNA specific dye and Fluo-3 as the calcium sensitive dye. We studied changes in calcium levels during the G1 phase of cell cycle by arresting cells with chemical agents such as staurosporine and hydroxyurea or by density dependent arrest of cell growth. We show that levels of calcium are lowest at the beginning of G1 phase but rise steadily with its progression and culminate at the G1/S border. Our results suggest that complex changes occur in calcium levels during the process of mitotic division. During progression of S and G2 phases, calcium levels decline and increase respectively. Our results offer a new methodology to estimate intracellular calcium levels in specific phases of the cell cycle. Based upon these results we propose a general scheme representing the changes in the intracellular calcium concentration during the progression of the cell cycle. © 1996 Wiley-Liss, Inc.

Calcium in biology

10.1002/(sici)1097-0320(19960501)24:1<55::aid-cyto7>3.0.co;2-h

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Profiling of normal and malignant breast tissue show CD44high/CD24lowphenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers

BMC Cancer (2013)

Hazem Ghebeh Ghida Majed Sleiman Pulicat Manogaran Amer Al‐Mazrou Eman Barhoush

Abstract Background Accumulating evidence supports cancer to initiate and develop from a small population of stem-like cells termed as cancer stem cells (CSC). The exact phenotype of CSC and their counterparts in normal mammary gland is not well characterized. In this study our aim was to evaluate the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts. Methods Freshly isolated cells from both normal and malignant human breasts were sorted using 13 widely used stem/progenitor cell markers individually or in combination by multi-parametric (up to 9 colors) cell sorting. The sorted populations were functionally evaluated by their ability to form colonies and mammospheres, in vitro . Results We have compared, for the first time, the stem/progenitor markers of normal and malignant breasts side-by-side. Amongst all markers tested, we found CD44 high /CD24 low cell surface marker combination to be the most efficient at selecting normal epithelial progenitors. Further fractionation of CD44 high /CD24 low positive cells showed that this phenotype selects for luminal progenitors within Ep-CAM high /CD49f + cells, and enriches for basal progenitors within Ep-CAM -/low /CD49f + cells. On the other hand, primary breast cancer samples, which were mainly luminal Ep-CAM high , had CD44 high /CD24 low cells among both CD49f neg and CD49f + cancer cell fractions. However, functionally, CSC were predominantly CD49f + proposing the use of CD44 high /CD24 low in combination with Ep-CAM/CD49f cell surface markers to further enrich for CSC. Conclusion Our study clearly demonstrates that both normal and malignant breast cells with the CD44 high /CD24 low phenotype have the highest stem/progenitor cell ability when used in combination with Ep-CAM/CD49f reference markers. We believe that this extensive characterization study will help in understanding breast cancer carcinogenesis, heterogeneity and drug resistance.

Stem cell marker

10.1186/1471-2407-13-289

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Citations (69)

Supplementary Figure 1 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Azhar R. Hussain Naif A. Al-Jomah Abdul K. Siraj Pulicat Manogaran Khalid Al-Hussein

Supplementary Figure 1 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Sanguinarine

Primary effusion lymphoma

10.1158/0008-5472.22370180.v1

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Vincristine and lomustine induce apoptosis and p21WAF1 up-regulation in medulloblastoma and normal human epithelial and fibroblast cells

Journal of Neuro-Oncology (2007)

Zakia Shinwari Pulicat Manogaran Salman A. Alrokayan Khaled Al‐Hussein Abdelilah Aboussekhra

Lomustine

10.1007/s11060-007-9502-4

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A Nuclear Protein Tyrosine Phosphatase Induces Shortening of G1 Phase and Increase in c-Myc Protein Level

Experimental Cell Research (2001)

Uma Ganapati Sanjeev Gupta Vegesna Radha Ch. Sudhakar Pulicat Manogaran

HeLa

10.1006/excr.2001.5158

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Citations (10)

Supplementary Figure 3 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Azhar Hussain Naif A. Al-Jomah Abdul K. Siraj Pulicat Manogaran Khalid Al-Hussein

Supplementary Figure 3 from Sanguinarine-Dependent Induction of Apoptosis in Primary Effusion Lymphoma Cells

Sanguinarine

Primary effusion lymphoma

10.1158/0008-5472.22370174.v1

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Modulation of alpha5beta1 integrin functions by the phospholipid and cholesterol contents of cell membranes.

PubMed (2000)

Praturi Gopalakrishna Sushma Chaubey Pulicat Manogaran Gopal Pande

Several modifications of the alpha5beta1 integrin, which alter its intracellular and extracellular interaction with fibronectin and other proteins, have been reported. However, the significance of the lateral mobility of integrin molecules in the plasma membrane, as a regulator of their distribution and function, is poorly understood. We examined this problem by increasing the cholesterol content of plasma membranes, and consequently modifying the fluidity of membrane phospholipids, in rat fibroblasts. Under these conditions, the clustering of alpha5beta1 integrin molecules in focal adhesions, their adhesion to the cell-binding domain of fibronectin, and their association with the cytoskeletal protein talin were significantly enhanced as compared to control cells. However, the activation of MAP-kinase pathways by the association of fibronectin with alpha5beta1 integrin, and its association with integrin-linked kinase (ilk), were suppressed. The treated cells also showed distinct changes in shape, and their actin stress fiber network was more dense and thick as compared to control cells. The changes in fluidity of phospholipids occurred differentially and fluidity of phosphatidyl-ethanolamine increased, while that of phosphatidyl-choline was reduced. Our results suggest that proteins in focal adhesions could be partitioned in specific lipid domains, which regulate specific aspects of alpha5beta1 integrin functions.

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Citations (45)