Xiliang Wang

Huazhong Agricultural University

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Dingren Bi

Huazhong Agricultural University

Deshi Shi

Huazhong Agricultural University

Yuncai Xiao

Huazhong Agricultural University

Xiue Jin

China Institute of Veterinary Drug Control

Zili Li

Huazhong Agricultural University

Zutao Zhou

Huazhong Agricultural University

Xiaowen Li

Jiaxing University

Sishun Hu

Huazhong Agricultural University

Yue Li

Henan University of Science and Technology

Babacar Ngom

Huazhong Agricultural University

Cooperative Institutions

Huazhong Agricultural University

107

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Sun Yat-sen University

Huazhong University of Science and Technology

Ministry of Agriculture and Rural Affairs

Chinese PLA General Hospital

Zhejiang University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

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Development and Identification of a Live Attenuated Influenza B Virus Vaccine Candidate

Penghui Yang Wenqi An Xinfu Shi Yueqiang Duan Deyan Luo

A cold-adapted (ca), temperature sensitive (ts), live attenuated influenza B virus strain B/Ann Arbor/1/66 was chosen influenza virus rescue research, in which six internal gene segments, PB 1, PB2, PA, NP, M, NS, were fully synthesized and nine amino acid substitutions were artificially alter by human intervention. The resultant B/Ann Arbor/1/66 plasmids were named as pAB121-PBI, pAB 122-PB2, pAB 123-PA, pAB 124-HA, pAB 125-NP, pAB126-NA, pAB 127-M and pAB 128-NS, respectively. A recombinant influenza A virus was previously generated entirely from cloned eDNA. An infectious recombinant influenza B virus was generated here, and designated as rMDV-B, by plasmid-based reverse genetics. The rMDV-B virus contained HA and NA genes from epidemic influenza B virus strain B/MalaysiaJ2506/2004 in the background of internal genes derived from influenza B vims strain B/Aim Arbor/1/66. HA titer of rMDV-B in MDCK cells and embryonated chicken eggs ranged from 1 64 to 1 512. The results may allow an effective live influenza B vaccine to be produced from a single master strain, providing a model for the design of future human influenza vaccines.

Embryonated

Reverse Genetics

Strain (injury)

Influenzavirus B

Attenuated vaccine

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Citations (0)

Using the optimal method—explained variance weighted genetic risk score to predict the efficacy of folic acid therapy to hyperhomocysteinemia

European Journal of Clinical Nutrition (2022)

Xiaorui Chen Xiaowen Huang Caifang Zheng Xiliang Wang Weidong Zhang

Hyperhomocysteinemia

10.1038/s41430-021-01055-5

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A Simple and Rapid Strip Test for Detection of Antibodies to Avian Infectious Bronchitis Virus

Journal of Veterinary Medical Science (2010)

Jinliang Zhang Yan Guo Yuncai Xiao Xiliang Wang Zhonghua Li

A simple gold-immunochromatographic assay (GICA) based on indirect reaction format was developed for rapid detection of antibodies against avian infectious bronchitis virus (IBV). The detection time of IBV IgG by the GICA was less than 10 min, whereas the HI test and the enzyme-linked immunosorbent assay (ELISA) require 1-2 hr. Reference sera against newcastle disease virus, infectious bursal disease, avian influenza virus H5 and H9 subtypes were all negatives for anti-IBV antibodies using the GICA. Compared with the HI, the sensitivity of the GICA was 92.11% and specificity was 81.71%. The agreement rate between the 2 tests was 85%. Compared with the ELISA, the sensitivity of the GICA was 92.31% and specificity was 97.06%. The agreement rate between the 2 tests was 95%. The GICA test strip is a reliable and useful tool for the on-site surveillance of anti-IBV antibodies.

Newcastle Disease

Avian infectious bronchitis

Infectious bursal disease

Gold standard (test)

10.1292/jvms.09-0528

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Citations (6)

The Accumulation and Subcellular Distribution of Arsenic and Antimony in Four Fern Plants

International Journal of Phytoremediation (2013)

Renwei Feng Xiliang Wang Chaoyang Wei Shuxin Tu

In the present study, Pteris cretica 'Albo-Lineata' (PC), Pteris fauriei (PF), Humata tyermanii Moore (HT), and Pteris ensiformis Burm (PE), were selected to explore additional plant materials for the phytoremediation of As and Sb co-contamination. To some extent, the addition of As and Sb enhanced the growth of HT, PE, and PF. Conversely, the addition of As and Sb negatively affected the growth of PC and was accompanied with the accumulation of high levels of As and Sb in the roots. The highest concentration of Sb was recorded as 6405 mg kg(-1) in the roots of PC, and that for As was 337 mg kg(-1) in the rhizome of PF. To some degree, As and Sb stimulated the uptake of each other in these ferns. Arsenic was mainly stored in the cytoplasmic supernatant (CS) fraction, followed by the cell wall (CW) fraction. In contrast, Sb was mainly found in the CW fraction and, to a lesser extent, in the CS fraction, suggesting that the cell wall and cytosol play different roles in As and Sb accumulation by fern plants. This study demonstrated that these fern plants show a good application potential in the phytoremediation of As and Sb co-contaminated environments.

Pteris vittata

Frond

10.1080/15226514.2013.773281

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Citations (50)

[Conditions for SARS-CoV cultivation and inactivation].

PubMed (2005)

Zhang Songle Lijuan Ma Guang Tian Liangyan Zhang Xueying Zhang

To study the method for cultivation and inactivation of SARS-CoV.In order to choose the sensitive cell strain and the best infection dose of the virus, Vero, Vero-E6 and 2BS cell lines were infected with SARS-CoV. The cultivation temperature was selected among 25 degrees C, 33 degrees C and 37 degrees C. The best inactivating time and effect were observed with beta-propiolactone whose concentration ranged from 1:2000 to 1:20,000 at room temperature.Vero and Vero-E6 cell lines were sensitive to SARS-CoV. The cytopathic changes of the cells were 75% at 37 degrees C in 5 percent CO2 incubator after infection. SARS-CoV was inactivated completely in beta-propiolactone (1:4000). The toxicity of beta-propiolactone was hydrolyzed completely when the inactivated virus was cultured for 16 hours at 2 degrees C, 8 degrees C and in water bath for 2 hours at 37 degrees C.The titer of SARS-CoV was the highest when it was cultured in Vero or Vero-E6 cells for 72 hours at 37 degrees C in 5 percent CO2 incubator. SARS-CoV was inactivated completely in beta-propiolactone when its concentration was 1:4000 and the interaction time was 1 hour.

Vero cell

Strain (injury)

Cytopathic effect

Incubator

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Development and validation of an immunochromatographic assay for rapid detection of sulfadiazine in eggs and chickens

Journal of Chromatography B (2006)

Xiliang Wang Kui Li Deshi Shi Xiue Jin Ning Xiong

Yolk

10.1016/j.jchromb.2006.10.038

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Citations (45)

Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling

Research Square (Research Square) (2024)

Yinghui Gong Yucheng He Yue Li Ying Wang Xiue Jin

Abstract CYP3A29, like human CYP3A4 is crucial for drug metabolism in pigs and some probiotics can regulate the expression of CYP3A in mammals. Here, we show that Enterococcus faecium HDRsEf1 significantly reduces CYP3A29 expression in pig intestinal tissues and epithelial cells, dependent on cell-cell contact. In IPEC-J2 cells, HDRsEf1 decreased the CYP3A29 promoter activity, RXR-α expression and mitigated the RXR-α or PXR-increased CYP3A29 expression. Both RXR-α/PXR over-expression synergistically increased CYP3A29 expression while RXR-α or PXR silencing reduced CYP3A29 expression. Co-immunoprecipitation revealed that RXR-α directly interacted with PXR. HDRsEf1, like a NF-kB inhibitor, significantly decreased the NF-kBp65 activation, RXR-α and CYP3A29 expression, which were abrogated by RXR-α silencing. HDRsEf1 increased A20 expression dependent on TLR1/2 expression. Therefore, HDRsEf1 inhibits the expression of CYP3A29 through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α signaling in pig intestinal tissues. Our findings suggest potential risks in the clinical application of probiotics.

Enterococcus faecium

10.21203/rs.3.rs-4192677/v1

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Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Insect Molecular Biology (2017)

Lin Qiu Pengwei Wang Tian Wu Baixue Li Xiliang Wang

Abstract Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis are highly toxic to lepidopteran pests. Strains of transgenic rice expressing cry genes have been developed that are resistant to rice pests. Understanding the mode of action of Cry toxins in rice pests will improve our ability to use them effectively as insecticides. In this study, we tested the hypothesis that alkaline phosphatases (ALPs) are involved in Cry1A, Cry2Aa and Cry1Ca toxicity in Chilo suppressalis , an important insect pest of rice crops in China. We first cloned three novel C . suppressalis alps ( Csalps ) from the larval midgut of C . suppressalis . RNA interference knockdown of six different Csalp genes ( Csalp1 , Csalp2 , Csalp3 , Csalp4 , Csalp5 and Csalp6 ) showed that knockdown of three of these, Csalp1 , Csalp2 and Csalp4 , reduced larval mortality to the transgenic rice strain TT51, which expresses a fusion protein of Cry1Ab and Cry1Ac, whereas suppression of Csalp1 , Csalp2 , Csalp3 , Csalp4 and Csalp6 transcripts decreased the susceptibility of larvae to the transgenic rice strain T2A‐1, which expresses cry2Aa . Moreover, downregulation of Csalp1 , Csalp2 , Csalp3 , Csalp4 and Csalp5 transcripts conferred significant tolerance to the transgenic rice strain T1C‐19, which expresses cry1Ca . These results suggest that these ALPs play a key role in the toxicity of Cry1A, Cry2A and Cry1C to C . suppressalis .

Chilo suppressalis

Bacillus thuringiensis

Genetically modified rice

Cry1Ac

10.1111/imb.12349

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Citations (18)

Reduced state carbon dots as both reductant and stabilizer for the synthesis of gold nanoparticles

Carbon (2013)

Xiliang Wang Yijuan Long Qinlong Wang Haijie Zhang Xiaoxiao Huang

Chloroauric acid

Sodium borohydride

Stabilizer (aeronautics)

Reducing agent

Sodium citrate

Sodium formate

Borohydride

Carbon fibers

4-Nitrophenol

10.1016/j.carbon.2013.07.104

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Citations (99)

Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs

PLoS ONE (2021)

Xiaoying Wu Wu Bei Yue Li Xiue Jin Xiliang Wang

Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola , may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E . sanguinicola . In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA , gelE , esp , agg , ace , efaAfm , efaAfs , ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E . thailandicus TC1 may be associated with a potential health risk.

Identification

10.1371/journal.pone.0254081

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Citations (7)