Haiyu Hong

Sun Yat-sen University

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Qintai Yang

Third Affiliated Hospital of Sun Yat-sen University

Yunping Fan

University of Chinese Academy of Sciences

Yueqi Sun

Sun Yat-sen University

Fenghong Chen

The First Affiliated Hospital, Sun Yat-sen University

Huabin Li

Eye & ENT Hospital of Fudan University

Kai Sen Tan

National University of Singapore

Jianbo Shi

Sun Yat-sen University

Jiaoping Mi

Sun Yat-sen University

De Yun Wang

National University Health System

Zhenpeng Liao

Fifth Affiliated Hospital of Sun Yat-sen University

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Sun Yat-sen University

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Fifth Affiliated Hospital of Sun Yat-sen University

The First Affiliated Hospital, Sun Yat-sen University

Third Affiliated Hospital of Sun Yat-sen University

Guangzhou Medical University

Capital Medical University

Shanghai Jiao Tong University

Southern Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Huazhong University of Science and Technology

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Role of IL‐25, IL‐33, and TSLP in triggering united airway diseases toward type 2 inflammation

Allergy (2020)

Haiyu Hong Shumin Liao Fenghong Chen Qintai Yang De Yun Wang

Under the concept of "united airway diseases," the airway is a single organ wherein upper and lower airway diseases are commonly comorbid. The upper and lower airways are lined with respiratory epithelium that plays a vital role in immune surveillance and modulation as the first line of defense to various infective pathogens, allergens, and physical insults. Recently, there is a common hypothesis emphasizing epithelium-derived cytokines, namely IL-25, IL-33, and TSLP, as key regulatory factors that link in immune-pathogenic mechanisms of allergic rhinitis (AR), chronic rhinosinusitis (CRS), and asthma, mainly involving in type 2 inflammatory responses and linking innate and adaptive immunities. Herein, we review studies that elucidated the role of epithelium-derived triple cytokines in both upper and lower airways with the purpose of expediting better clinical treatments and managements of AR, CRS, asthma, and other associated allergic diseases via applications of the modulators of these cytokines.

Interleukin 22

Proinflammatory cytokine

10.1111/all.14526

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Citations (173)

Downregulation and Aberrant Localization of Forkhead Box J1 in Allergic Nasal Mucosa

International Archives of Allergy and Immunology (2018)

Yang Peng Zhuo Chen Wei‐jie Guan Zhenchao Zhu Kai Sen Tan

Background: Forkhead box J1 (FOXJ1) plays pivotal roles in motile cilia formation. However, it remains unclear whether abnormal expression or localization of FOXJ1 in nasal mucosa tissues is associated with allergic rhinitis (AR), in which impaired mucociliary clearance is implicated. Objective: We sought to investigate the expression and localization of FOXJ1 in inferior turbinate from patients with AR and controls. Methods: We assayed mRNA levels of FOXJ1, DNAI1, DNALI1, and DNAH9 by using whole-genome expression array and quantitative real-time polymerase chain reaction. We elucidated the localization of FOXJ1 by using immunofluorescence assays in paraffin sections and primary single cells. Four patterns of FOXJ1 localization (normal, N; intermediate, I; mislocalization, M; absence, A) were defined. We developed a semiquantitative scoring system to elucidate their localization in 5 areas per paraffin section, with individual sections being assigned a score between 0 and 2. Results: The mRNA levels of FOXJ1, DNAI1, DNALI1, and DNAH9 were significantly reduced in patients with AR compared with controls (all p < 0.05). The median (1st and 3rd quartile) of the FOXJ1 score was 0.4 (0.0 and 0.85) in patients with AR, and 0.2 (0.0 and 0.4) in controls (p < 0.05). For primary cytospin samples, the mean percentages of FOXJ1 localization patterns N, I, M, and A were 46.7, 10.0, 30.0, and 26.7% in patients with AR, and 82.5, 5.0, 5.0, and 7.5% in controls, respectively (p < 0.05). Conclusion: Downregulation and aberrant localization of FOXJ1 may be crucial characteristics of the allergic nasal mucosa.

Forkhead Transcription Factors

10.1159/000488014

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Citations (16)

miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8

Mediators of Inflammation (2015)

Zhongsi Hong Haiyu Hong Jian Liu Xiaobin Zheng Mingxing Huang

Aims . This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a. Materials and Methods . miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively. Results . The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects. Conclusions . This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

10.1155/2015/629862

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Citations (14)

Arsenic trioxide suppresses cell growth and migration via inhibition of miR-27a in breast cancer cells

Biochemical and Biophysical Research Communications (2015)

Shunhua Zhang Cong Ma Haijie Pang Fanpeng Zeng Long Cheng

Arsenic Trioxide

Gentamicin protection assay

MTT assay

10.1016/j.bbrc.2015.11.071

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Citations (47)

The top 100 most influential articles in olfactory disorder: a bibliometric analysis

Zenodo (CERN European Organization for Nuclear Research) (2022)

Tengyu Chen Haiyu Hong Dan Li Caishan Fang Man-qing Lin

Introduction: Studies on olfactory disorder have varied considerably in content and field over the past four decades. Influential publications of olfactory disorder have not been analyzed quantitatively. This study aimed to analyze the top 100 most influential articles in olfactory disorder through bibliometrics. Methods: The researchers searched the Web of Science Core Collection for publications from 1980 to August 31, 2021. The top 100 highly cited articles were screened in accordance with the inclusion and exclusion criteria. The journal impact factor and the SCImago Journal Rank indicator were searched for the journal in which the top-cited articles were published. The researchers analyzed the data via Microsoft Excel and SPSS 24.0 and used VOSviewer software for data visualization. Results: The total citations of the top 100 articles have increased exponentially, especially in the past 2 years. The journal LARYNGOSCOPE contributed the most influential papers (n=6). The United States published the most top 100 articles (n=52), followed by Germany and the United Kingdom. The University of Pennsylvania published the most influential studies, with a total of 3,852 citations. Richard L. Doty and Thomas Hummel contributed the most influential literature, and the top 100 studies also cited their research frequently. The most important articles in the field of OD mainly focused on COVID-19, Parkinson's disease and olfactory tests. Conclusion: Neurodegenerative diseases and COVID-19-related olfactory disorders have been considered main topics over the past 40 years. This study identified the most influential articles for olfactory disorder researchers, providing guidance for their research.

10.5281/zenodo.6632051

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Citations (0)

Non-invasive treatment to grade 1 essential hypertension by percutaneous laser and electric pulse to acupoint with music: A randomized controlled trial

Chinese Journal of Integrative Medicine (2016)

Hongrui Zhan Zhongsi Hong Yi-Shen Chen Haiyu Hong Zebin Weng

10.1007/s11655-016-2502-5

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Citations (6)

Microarray Assay Reveals Ciliary Abnormalities of the Allergic Nasal Mucosa

American Journal of Rhinology and Allergy (2019)

Yang Peng Wei‐jie Guan Zhenchao Zhu Kai Sen Tan Zhuo Chen

Background Gene expression patterns (particularly, cilia-associated genes) of nasal mucosa, the first-line defense system, in allergic rhinitis (AR) are not well understood. Objective We sought to screen for AR-associated genes in inferior turbinate (IT) from patients with AR, and to validate the expression of common cilia-related genes and ciliary shedding. Methods Prime View™ Human Gene Expression Array, which consisted of more than 530 000 probes covering more than 36 000 transcripts and variants, was employed to compare individual gene expression of ITs from control subjects (n = 11) and patients with AR (n = 19). Gene ontology (GO) analysis was performed with Cytoscape software. Eight of the common cilia-related genes were validated with quantitative polymerase chain reaction. We applied a semiquantitative scoring system for immunofluorescence assay to demonstrate ciliary shedding in 5 areas per paraffin section, with individual sections being scored between 0 (normal ciliary distribution) and 1 (ciliary shedding). Results Compared with control subjects, 160 (38 upregulated and 122 downregulated) genes were differentially expressed for at least 2 folds (all P < .05) in AR. Seven GO categories were significantly enriched, 4 of which were related to cilium assembly and motility. Quantitative polymerase chain reaction validated the predicted direction of change for common cilia-related gene expression. The ciliary distribution score was significantly higher (more prominent ciliary shedding) in AR than in controls ( P < .05). Conclusion The significant aberrant cilia-related gene expression, revealed by microarray assays, might be the critical driver of AR where ciliary shedding is prominent.

Immunofluorescence

10.1177/1945892419871795

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Citations (8)

Prediction models for postoperative uncontrolled chronic rhinosinusitis in daily practice

The Laryngoscope (2018)

Xiaoyao Tao Fenghong Chen Yueqi Sun Shulian Wu Haiyu Hong

The European Position Paper on Rhinosinusitis and Nasal Polyps proposes an assessment of clinical control of chronic rhinosinusitis (CRS). However, there are limited data about the percentage of postoperative control, and no prediction models for uncontrolled CRS have been reported. The aim of the study was to develop prediction models for postoperative uncontrolled CRS.Retrospective case series.Patients (n = 136) who had undergone endoscopic sinus surgery at least 1 year prior to the study were recruited to assess the clinical control. Risk factors were determined by logistic models and presented as odds ratio (OR) with a 95% confidence interval. Receiver operating characteristics curves were constructed to set the cutoff points and create predictive models.Approximately 47.8% of patients had controlled, 22.1% partially controlled, and 30.1% uncontrolled CRS. Univariate regression models revealed the risk factors for uncontrolled CRS: tissue eosinophilia, blood eosinophilia, high computed tomography (CT) score, bilateral disease, asthma, and allergic rhinitis. Multiple regression models found tissue eosinophil ratio >0.206 (OR: 12.96, P = .001) or blood eosinophil ratio >0.025 (OR: 4.56, P = .003), Lund-Mackay (LM) score ≥ 15 (OR: 15.50, P < .001) and CT ethmoid (E) score ≥ maxillary (M) score (OR: 3.51, P = .037) were independent risk factors. We generated a pathological model (tissue eosinophil ratio and LM score) and a clinical model (blood eosinophil ratio, LM score and E ≥ M score) to categorize CRS into mild, moderate, and severe.This research provides simplified and efficient prediction models for uncontrolled CRS. It may help otolaryngologists to predict the prognosis before surgery in daily practice.2b Laryngoscope, 128:2673-2680, 2018.

Nasal Polyps

10.1002/lary.27267

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Citations (44)

Expression of leukotriene and its receptors in eosinophilic chronic rhinosinusitis with nasal polyps

International Forum of Allergy & Rhinology (2015)

Xingmei Wu Haiyu Hong Kejun Zuo Miaomiao Han Jian Li

Background Cysteinyl leukotriene (LT) has been proposed in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This study sought to examine the expression of the LT receptor (LTR) in CRSwNP patients and evaluate the potential role of LTR antagonist (LTRA) in the management of eosinophilic CRSwNP (ECRS) patients. Methods Nasal polyps and uncinate process tissues were collected from 18 ECRS patients, 13 non‐eosinophilic CRSwNP (non‐ECRS) patients, and 16 control subjects. The messenger RNA (mRNA) and protein expression of LTR (cysteinyl leukotriene receptor 1 [CysLT1R] and cysteinyl leukotriene receptor 2 [CysLT2R]) was examined using quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR), immunohistochemistry, and Western blot analysis. Moreover, the effects of LTRA and steroids on total nasal symptom scores (TNSS) of uncontrolled ECRS patients were evaluated. Results The mRNA and protein expression of CysLT1R and CysLT2R was significantly increased in polyp tissues compared with healthy controls ( p < 0.05). Compared with the non‐ECRS subset, the ECRS subset showed significantly increased expression of CysLT1R and CysLT2R, as well as leukotriene C4 (LTC4) and leukotriene D4 (LTC4) levels ( p < 0.05). Moreover, combined LTRA and steroids significantly decreased TNSS more than steroids alone in uncontrolled ECRS patients ( p < 0.01). Conclusion Our findings indicate that LTR was differentially expressed between ECRS and non‐ECRS patients, and that LTRA may be used as an additional therapy for ECRS patients.

Nasal Polyps

Leukotriene receptor

Pathogenesis

10.1002/alr.21625

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Citations (23)

Tumor Microbiome in Nasopharyngeal Carcinoma and Its Association With Prognosis

Frontiers in Oncology (2022)

Guihua Zhong Wei Wei Wei Liao Rong Wang Yingpeng Peng

Introduction Previous studies have reported a close relationship between cancer and microbes, particularly gut and tumor microbiota; however, the presence of tumor microbiome in nasopharyngeal carcinoma (NPC) and its role in the prognosis of NPC remain unclear. Methods We collected 64 samples including tissues from 50 patients with NPC (NPC group) and 14 patients with chronic nasopharyngitis (control group) receiver operating characteristics and we applied 16S ribosome RNA gene sequencing of all samples to assess microbiome profiles and immunohistochemistry to detect tumor microbiome in NPC. Results Patients in the control group harbored higher species diversity than those in the NPC group; however, the beta diversity was more distinct in the NPC group. In total, three genera with statistically significant differences between the two groups were identified. The area under the receiver operating characteristics (ROC) curve (AUC) was calculated using the relative abundance of these three significant genera, and a value of 0.842 was achieved. Furthermore, Turicibacter was confirmed as a potentially independent prognostic factor for NPC patients, and the progression-free survival (PFS) was markedly prolonged in patients with a low relative abundance of Turicibacter compared to patients with a high relative abundance of this genus (cutoff: 0.0046, hazard ratio: 5.10, 95% confidence interval: 2.04–12.77, p = 0.004). Conclusions The present study provided strong evidence of a correlation between tumor microbiome and NPC; the tumor microbiome may be considered a biomarker for early NPC diagnosis. Turicibacter potentially served as a independently prognostic indicator for NPC patients.

10.3389/fonc.2022.859721

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Citations (17)