Objective To explore the value of phage-based splitting assay in fast detecting mycobacterium tuberculosis in the pleural fluid.Methods 116 pleural fluid of tuberculosis were detected with phage-based splitting assay,smear method and L-J cultivation.Results 79.3% of samples was positive by phage-based splitting assay,which was significantly deviated with smear method(31.9%) but not significantly deviated with L-J cultivation(74.1%).Conclusion Phage-based splitting assay is fast、safe and convenient method to detect the viable organism of mycobacterium tuberculosis with high sensitivity and specificity.
Our understanding of the human immune system's response to viral respiratory tract infections (VRTIs) and vaccines, including the molecular mechanisms and correlates of protection, remains incomplete. Extensive transcriptomic data from inoculation and vaccination studies have been deposited in publicly available databases. However, these studies are often separate and difficult to locate. We provide a curated compendium of public gene expression data repositories for researchers to reanalyze transcriptomes from human whole blood, peripheral blood mononuclear cells (PBMCs), and nasal swab samples. This enables the study of transcriptional responses to viral inoculation or vaccination. This collection includes 18 datasets from inoculation studies and 37 datasets from vaccination studies, sourced from the NCBI Gene Expression Omnibus (GEO), ImmPort Shared Data, and ArrayExpress.
Objective In order to research on the mechanisms of the decrease of hepatitis C viral (HCV) RNA with methylene blue photochemistry (MB-P) treatment, and to provide theoretic bases for clinic applications on viral inactivation of blood products. Methods The conservative 5′-NCR of HCV genome RNA was selected as the research template. After being exposed in the light for different periods with methylene blue,HCV RNA in the plasma was extracted and analyzed quantitatively with fluorescence quantitation PCR. Meanwhile, the same experiment was performed on in vitro transcripted 5′-NCR of HCV RNA, which was resolved in plasma substitute (hydroxyethyl starch 20-sodium chloride solution) and PBS buffer without any component of protein. Results The quantity of HCV RNA decreased in a logarithmic way with MB-P treatment, and the efficiency of inactivation on in vitro transcripted HCV RNA was lower than that of HCV virus in plasma.Conclusion With light exposure,methylene blue could act on viral nucleic acid and break RNA chain to decrease the quantity of viral RNA, whereafter inactivate the virus finally.
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