The authors investigated possible toxic side effects of epiretinal triamcinolone acetonide deposits at the posterior pole after an intravitreal injection in both a vitrectomized and a non-vitrectomized eye. The vitrectomized eye developed massive epiretinal triamcinolone acetonide deposits at the posterior pole that were less pronounced in the non-vitrectomized eye. After resolution of the deposits, no morphologic signs of retinal toxicity were apparent. Mild scattered visual field defects did not correlate with the localization of the triamcinolone acetonide deposits. However, because recent in vitro studies indicate potential cytotoxicity, patients should be instructed to keep their heads in an upright position after intravitreal triamcinolone acetonide injection to avoid deposits at the posterior pole. [<CITE>Ophthalmic Surg Lasers Imaging</CITE> 2007;38:238-241.] <H4>AUTHORS</H4> From the Department for Vitreoretinal Surgery, University Eye Hospital, Tüebingen, Germany. Accepted for publication February 10, 2007. Address correspondence to Gesine B. Jaissle, MD, Department for Vitreoretinal Surgery, University Eye Hospital, Schleichstr. 12, 72076 Tüebingen, Germany.

Acetonide

Posterior pole

10.3928/15428877-20070501-10

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Citations (8)

Sodium hyaluronate gels as a drug‐release system for corticosteroids: release kinetics and antiproliferative potential for glaucoma surgery

Acta Ophthalmologica (2008)

Martin S. Spitzer Efdal Yoeruek Radosław Kaczmarek Ana Sierra Sabine Aisenbrey

To evaluate the release kinetics, biocompatibility and antiproliferative potential of a concentrated hydrophilic steroid formulation from commercially available sodium hyaluronate gels as a potential adjunct in glaucoma surgery.Dexamethasone and sodium hyaluronate 1% (Healon) and sodium hyaluronate 2.3% (Healon 5) were mixed to yield sodium hyaluronate formulations containing dexamethasone in concentrations of 4-20 mg/ml (7.7-38 mm). Non-cumulative and cumulative release into balanced salt solution (BSS) or phosphate buffered saline (PBS) was measured spectrophotometrically over 2-6 days. For cytotoxicity assays, human tenon fibroblasts (HTFB) and human retinal pigment epithelium cells (ARPE19) were cultured in a serum-deficient medium to ensure a static milieu; 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay and Live/Dead cell-mediated cytotoxicity assay were used to exclude cytotoxicity. Cellular proliferative activity was monitored by 5'-bromo-2'-deoxyuridine (BrdU)-incorporation into cellular DNA.The release kinetics from sodium hyaluronate 1% and 2.3% were almost identical. Steady state was achieved after approximately 44 hrs in non-cumulative measurements. The release plotted as a function of the square root of time was consistent with a largely diffusion-controlled release system. No cytotoxicity could be observed. Dexamethasone-loaded sodium hyaluronate showed a significant antiproliferative effect on HTFB and ARPE19 cells.Dexamethasone-loaded sodium hyaluronate shows extended release of steroid over almost 2 days in concentrations high enough to inhibit the proliferation of HTFB and RPE cells without evoking cytotoxic effects. Thus, this formulation may be an easy-to-prepare adjunct in glaucoma surgery or other procedures in which cellular growth inhibition is desired.

Sodium hyaluronate

Balanced salt solution

Biocompatibility

10.1111/j.1755-3768.2007.01149.x

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Citations (23)

Suprachoroidal hydrogel buckling as a surgical treatment of retinal detachment - biocompatibility and first experiences

Investigative Ophthalmology & Visual Science (2016)

Kai Januschowski Karl Boden Peter Szurman

Biocompatibility

Source

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Zur Häufigkeit der exzessiven Verdickung der epithelialen Basalmembran beim Keratokonus

Klinische Monatsblätter für Augenheilkunde (2006)

Jens Martin Rohrbach Peter Szurman M El-Wardani Matthias Grüb

Hintergrund: Der Keratokonus ist trotz einer Vielzahl von morphologischen und biochemischen Befunden ätiologisch weiterhin ungeklärt. Während die primären Störungen bei diesem Krankheitsbild überwiegend im vorderen Stroma gesucht und gesehen werden, spielt für einige Autoren das Epithel eine große, wenn nicht gar die entscheidende pathogenetische Rolle. Damit rückt auch die epitheliale Basalmembran in das Interesse der Keratokonus-Forschung. Material und Methode: In die Studie eingeschlossen wurden 55 Patienten, bei denen eine perforierende Keratoplastik wegen eines Keratokonus durchgeführt worden war. Die Hornhautscheibchen wurden in üblicher Weise für die Lichtmikroskopie prozessiert und mit PAS gefärbt. Bei der mikroskopischen Betrachtung wurde das gesamte Explantat beurteilt, besonderes Augenmerk aber auf die Verhältnisse an der epithelialen Basalmembran gelegt. Zur Kontrolle wurden 10 normale Hornhäute von Augen, die wegen eines Aderhautmelanoms enukleiert werden mussten, untersucht. Ergebnisse: Bei 3 Patienten (5,5 %) fand sich eine signifikante, lineare Basalmembran-Verdickung (Dicke der epithelialen BM mehr als 5 % aber weniger als 30 % der Dicke der Bowman-Schicht). Nur bei einer Patientin (1,8 %) zeigte sich eine exzessive Basalmembran-Verdickung (Dicke der epithelialen BM mehr als 30 % der Dicke der Bowman-Schicht), die fokal (zentral) mit epithelwärts gerichteten, Guttata-ähnlichen Veränderungen der BM („inverse Cornea guttata”) vergesellschaftet war. Die Basalmembranverdickung war in allen Fällen parazentral stärker ausgeprägt als im Zentrum des Konus. In der Kontrollgruppe war bei keinem Auge eine signifikante BM-Verdickung feststellbar. Schlussfolgerungen: Eine erhebliche Verdickung der epithelialen Basalmembran ist beim Keratokonus gelegentlich lichtmikroskopisch nachweisbar, insgesamt aber selten und nicht zuletzt aus diesem Grunde weit eher als unspezifisches Epi-Phänomen denn als Konus-verursachendes Ereignis anzusehen. Die beobachtete „inverse Cornea guttata” stellt einen ungewöhnlichen Einzelbefund dar.

10.1055/s-2006-927096

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Citations (8)

Trichostatin A induces cell death at the concentration recommended to differentiate the RGC-5 cell line

Neurochemistry International (2012)

Sven Schnichels Maximilian Schultheiß Johanna Hofmann Peter Szurman Karl Ulrich Bartz‐Schmidt

Trichostatin A

Viability assay

Propidium iodide

10.1016/j.neuint.2012.02.017

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Citations (15)

Erratum: Sicherheit, Effektivität und protektive Wirkung einer Parazentese bei intravitrealen Injektionen: „Eine Lanze für die Parazentese brechen“

Klinische Monatsblätter für Augenheilkunde (2020)

Michael Koss Matthias Bolz Albert J. Augustin Frank Koch Peter Szurman

Correction to: Sicherheit, Effektivität und protektive Wirkung einer Parazentese bei intravitrealen Injektionen: „Eine Lanze für die Parazentese brechen“Klin Monbl Augenheilkd 2020; 237(01): 90-94DOI: 10.1055/a-0972-9747

10.1055/a-1185-0060

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Comparing the Effects of Two Different Irrigation Solutions on an Isolated Perfused Vertebrate Retina

Ophthalmic Research (2012)

Kai Januschowski Ramin Maddani Sebastian Mueller Matthias Lüeke Martin S. Spitzer

Purpose: To compare the effect of a taurine-containing intraocular irrigation solution (PuriProtect™) to a standard irrigation solution (BSS™) we evaluated the retinal function using an electroretinogram (ERG) and analyzed the survival of retinal ganglion cells on isolated whole mount retinas. Materials and Methods: During ERG recordings, each irrigation solution was superfused for 45 min with the relevant irrigation solution. To investigate the effects on photoreceptor function, 1 mM asparate was added to obtain a-waves. The recovery of the a- and b-wave was monitored after superfusing the retinas with standard medium again. To evaluate the percentage of dead ganglion cells, retinas were stored for 24 h at 4°C in darkness and after staining the retinas with ethidium homodimer-1 the retinas were analyzed using fluorescence microscopy. Results: The application of standard medium supplemented with 2 mM taurine resulted in a significant increase of the b-wave amplitude compared to standard medium alone. The a-wave amplitudes showed no significant changes under taurine supplementation. Compared to standard medium BSS showed no significant decrease in b-wave amplitudes, but a significant decrease in a-wave amplitudes. In contrast to BSS there were no significant changes in the a- or b-wave amplitudes detectable after the application of PuriProtect. At the end of the washout period no significant changes in a- or b-wave amplitudes were recorded for any tested irrigation solution. Retinas stored for 24 h in PuriProtect or in standard medium with taurine had a statistically significant smaller amount of dead cells than retinas stored in standard medium without taurine supplementation. Conclusions: BSS does not seem to be an ideal irrigation solution, because it compromises the a-wave in the ERG. In contrast to BSS, PuriProtect showed no significant impact on the ERG and showed a better long-term effect on ganglion cell survival. Taurine supplementation, therefore, seems to be neuroprotective and its supplementation to an intraocular irrigation solution favorable for the retina.

Erg

Washout

Darkness

10.1159/000335365

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Citations (3)