Ligeng Li

Tianjin University

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Hua Tian

Tianjin University

Changhong Huo

Hebei Medical University

Qing‐Wen Shi

Nanjing Boiler and Pressure Vessel Inspection Institute

Manli Zhang

First Hospital of Jilin University

Lingfeng Shi

University of Science and Technology of China

Gequn Shu

Tianjin University

Xuan Wang

Beihang University

Hiromasa Kiyota

Okayama University

Gequn Shu

Tianjin University

Yu‐Cheng Gu

Syngenta (United Kingdom)

Cooperative Institutions

Tianjin University

Hebei Medical University

Chinese Academy of Sciences

University of Science and Technology of China

Memorial Sloan Kettering Cancer Center

Chinese Academy of Medical Sciences & Peking Union Medical College

Central South University

Sun Yat-sen University

Shanghai Jiao Tong University

Tohoku University

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Modulation of Thermal Induction of hsp70 Expression by Ku Autoantigen or Its Individual Subunits

Molecular and Cellular Biology (1996)

Shaohua Yang André Nussenzweig Ligeng Li Dooha Kim Honghai Ouyang

Previously, we proposed a dual control mechanism for the regulation of the heat shock response in mammalian cells: a positive control mediated by the heat shock transcription factor HSF1 and a negative control mediated by the constitutive heat shock element-binding factor (CHBF). To study the physiological role of CHBF in the regulation of heat shock response, we purified CHBF to apparent homogeneity and showed it to be identical to the Ku autoantigen, a heterodimer consisting of 70-kDa (Ku-70) and 86-kDa (Ku-80) polypeptides. To study further the functional significance of Ku/CHBF in the cellular response to heat shock, we established rodent cell lines that stably and constitutively overexpressed one or both subunits of the human Ku protein, and examined the thermal induction of hsp70 and other heat shock proteins in these Ku-overexpressing ing cells. We show that expression of the human Ku-70 and Ku-80 subunits jointly or of the Ku-70 subunit alone specifically inhibits heat-induced hsp70 expression. Conversely, expression of human Ku-80 alone does not have this effect. Thermal induction of other heat shock proteins in all of the Ku-overexpressing cell lines appears not to be significantly affected, nor is the state of phosphorylation or the DNA-binding ability of HSF1 affected. These findings support a model in which hsp70 expression is controlled by a second regulatory factor in addition to the positive activation of HSF1. The Ku protein, specifically the Ku-70 subunit, is involved in the regulation of hsp70 gene expression.

HSF1

Heat shock factor

HSPA4

10.1128/mcb.16.7.3799

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Citations (50)

Demonstration of a small‐scale power generator using supercritical CO₂

Carbon Energy (2024)

Ligeng Li Hua Tian Xin Lin Xianyu Zeng Yurong Wang

Abstract The supercritical CO 2 (sCO 2 ) power cycle could improve efficiencies for a wide range of thermal power plants. The sCO 2 turbine generator plays an important role in the sCO 2 power cycle by directly converting thermal energy into mechanical work and electric power. The operation of the generator encounters challenges, including high temperature, high pressure, high rotational speed, and other engineering problems, such as leakage. Experimental studies of sCO 2 turbines are insufficient because of the significant difficulties in turbine manufacturing and system construction. Unlike most experimental investigations that primarily focus on 100 kW‐ or MW‐scale power generation systems, we consider, for the first time, a small‐scale power generator using sCO 2 . A partial admission axial turbine was designed and manufactured with a rated rotational speed of 40,000 rpm, and a CO 2 transcritical power cycle test loop was constructed to validate the performance of our manufactured generator. A resistant gas was proposed in the constructed turbine expander to solve the leakage issue. Both dynamic and steady performances were investigated. The results indicated that a peak electric power of 11.55 kW was achieved at 29,369 rpm. The maximum total efficiency of the turbo‐generator was 58.98%, which was affected by both the turbine rotational speed and pressure ratio, according to the proposed performance map.

Electric generator

Maximum power principle

Ram air turbine

10.1002/cey2.428

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Citations (2)

Forsythia suspensa(Thunb)Vahl葉からのポリヒドロキシトリテルペノイド類とフェノール性成分

Journal of Agricultural and Food Chemistry (2016)

Ying Ge Wang Yazhen Pingping Chen Yufang Wang Hou Congcong

Forsythia

Source

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A new taxane from Taxus canadensis needles

Chemistry of Natural Compounds (2012)

Manli Zhang Zhi‐Yu Ni Yufang Wang Mei Dong Ligeng Li

Taxane

10.1007/s10600-012-0102-y

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Citations (5)

A New pseudo-Alkaloid Taxane and a New Rearranged Taxane from the Needles of Taxus canadensis

Zeitschrift für Naturforschung B (2008)

Manli Zhang Changhong Huo Mei Dong Ligeng Li Françoise Sauriol

A new taxane with an amino side chain on C-5 and a new 11(15→1)abeotaxane having a tetrahydrofuran ring along carbon atoms C-2, C-3, C-4, C-20 identified for the first time from the needles of the Canadian yew, Taxus canadensis. Their structures were characterized as 2α,7β ,9α,10β ,13-pentaacetoxy-11β -hydroxy-5α-(2´ -hydroxy,3'-N,N-dimethylamino-3´- phenyl)-propionyloxytaxa-4(20),12-diene (1) and 13α,20β -diacetoxy-5α,7β ,9α,10β -tetrahydroxy- 2α,20-epoxy-11(15→1)abeotaxa-11,15-diene (2) on the basis of 1D, 2D NMR spectroscopy and high-resolution FABMS analysis. Taxane 1 contains a rare C-12, C-13 double bond and a basic side chain, while taxane 2 bears a rare isopropenyl group at C-1.

Taxane

Tetrahydrofuran

10.1515/znb-2008-0814

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Citations (5)

Effects of external perturbations on dynamic performance of carbon dioxide transcritical power cycles for truck engine waste heat recovery

Energy (2018)

Xiaoya Li Hua Tian Gequn Shu Hu Chen Rui Sun

Overshoot (microwave communication)

Thermal expansion valve

Transcritical cycle

Settling time

Valve timing

10.1016/j.energy.2018.08.137

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Citations (19)

PPARγ Agonist from Chromolaena odorata

Journal of Natural Products (2012)

Manli Zhang Dianne Irwin Xiaoning Li Françoise Sauriol Xiaowei Shi

A phytochemical investigation of Chromolaena odorata resulted in the isolation of five new compounds, 5aα,6,9,9aβ,10-pentahydro-10β-hydroxy-7-methylanthra[1,2-d][1,3]dioxol-5-one (1), 1,2-methylenedioxy-6-methylanthraquinone (2), 3-hydroxy-1,2,4-trimethoxy-6-methylanthraquinone (3), 3-hydroxy-1,2-dimethoxy-6-methylanthraquinone (4), and 7-methoxy-7-epi-medioresinol (5), together with 12 known compounds, odoratin (6), 3β-acetyloleanolic acid (7), ursolic acid (8), ombuin (9), 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone (10), (−)-pinoresinol (11), austrocortinin (12), tianshic acid (13), cleomiscosin D (14), (−)-medioresinol (15), (−)-syringaresinol (16), and cleomiscosin A (17). All the compounds were evaluated for their PPARγ transactivation activity, and compound 6 showed moderate activity with an EC50 value of 3.10 μM.

Chromolaena odorata

Phytochemical

Pinoresinol

Ursolic acid

10.1021/np300386d

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Citations (45)

Optimum pressure control with three controllable ejectors of a CO2 multi-ejector refrigeration system

International Journal of Refrigeration (2023)

Ligeng Li Hua Tian Kai Liu Yibo Wu Xuan Wang

Bandwidth throttling

Coefficient of performance

Transcritical cycle

Lift (data mining)

Overall pressure ratio

10.1016/j.ijrefrig.2023.10.015

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Citations (6)

Identification of in vitro and in vivo metabolites of alantolactone by UPLC-TOF-MS/MS

Journal of Chromatography B (2016)

Donggui Yao Zhe Li Changhong Huo Yufang Wang Yibing Wu

Biotransformation

Metabolic pathway

10.1016/j.jchromb.2016.08.034

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Citations (24)

Enhancement of nitrocaphane cytotoxicity by hyperthermiain vitro

International Journal of Hyperthermia (1989)

Ligeng Li Guoli Shen Hong Zhang Dingjiu Li

HEp-2 cells were treated with hyperthermia (39-44 degrees C) and nitrocaphane (NC) at various time intervals. A 1 h exposure to 39 degrees C and 41 degrees C was non-lethal to cells, but it did potentiate the cell killing of NC (1.0 micrograms/ml). It was further shown that the sequence between heat and the drug can affect cell survival. Cell killing effect was decreased when heat was given before or after administration of drug. In contrast the simultaneous administration of these two modalities was synergistic. Maximal thermotolerance of HEp-2 cells was developed using an 8-h interval at 37 degrees C between the cell exposures to two equal thermal doses (44 degrees C, 30 min). HEp-2 cells became thermotolerant when preheated for 30 min at 44 degrees C followed by a 10-h interval at 37 degrees C. The thermotolerant cells showed resistance to subsequent heat at 44 degrees C (D0 = 2.26 h, control D0 = 0.38 h), to subsequent NC treatment, and to heat combined with NC. However, in the thermotolerant cells, cytotoxicity of NC was still enhanced by hyperthermia.

Cell Survival

10.3109/02656738909140476

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Citations (1)