María Belén Colavolpe

Instituto Tecnológico de Chascomús

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Edgardo Albertó

Instituto Tecnológico de Chascomús

Oscar A. Ruíz

Instituto Tecnológico de Chascomús

Rita Costa

University of Lisbon

Susana Serrazina

University of Lisbon

Isabel Videira e Castro

Instituto Nacional de Investigação Agrária e Veterinária

Rui Malhó

University of Lisbon

Santiago Jaramillo Mejía

National University of General San Martín

Patrícia Fernandes

State University of New York

Márcia de Castro Silva

Instituto Nacional de Investigação Agrária e Veterinária

Santiago Javier Maiale

Instituto Tecnológico de Chascomús

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

Instituto Tecnológico de Chascomús

National University of General San Martín

Instituto Nacional de Investigação Agrária e Veterinária

Universidad Autónoma de Guerrero

Universidade Nova de Lisboa

University of Lisbon

Universitat de València

University of Buenos Aires

Consejo Superior de Investigaciones Científicas

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Antibiotic Compounds Present in Suppressive Soils against Fungal and Oomycetes Plant Pathogens: A Mini-Review

Open Access Journal of Biogeneric Science and Research (2020)

María Belén Colavolpe

Background:The plants are part of a complex environment that includes microorganisms in the soil and covers different ecological interactions.Filamentous plant pathogens can attack plants to obtain nutrients for growth and reproduction.The soil within its complex microbial communities and the production of bioactive compounds; currently represent an interesting scientific subject for searching novel bioproducts of biotechnological interest.Certain bacteria of the microbiota can protect plants against pathogens using different mechanisms.The protective effect can be directly due to antibiotic compounds production or indirect by promoting plant defense as induced systemic resistance.This minireview describes some aspects of the direct effect: the production of antibiotic compounds by antagonist bacteria, mainly of the genus Bacillus, Burkholderia, and Pseudomonas in suppressive soils.

10.46718/jbgsr.2020.05.000128

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Burkholderia sp. Strain AU4i Promotes Arabidopsis Growth and Increases Plant Defence Response to Necrotrophic Pathogens

Journal of Plant Growth Regulation (2020)

María Belén Colavolpe Natalia M. Villarreal Silvia E. Langer Fernando Matías Romero Gustavo A. Martı́nez

Sclerotinia Sclerotiorum

Jasmonate

Methyl jasmonate

10.1007/s00344-020-10238-6

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Efecto de los progestágenos Crestar ® y CIDR ® en la inducción y sincronización de celos en ganado cebuíno, en la hacienda las Mercedes, Departamento de Francisco Morazán, Honduras

P. Martínez María Belén Colavolpe

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Castanea crenata Ginkbilobin2-like as a resistance gene to Phytophthora cinnamomi infection

Acta Horticulturae (2024)

Susana Serrazina M. T. Martínez Paulo Manuel Pêgo‐Fernandes María Belén Colavolpe Fernanda Furlan Gonçalves Dias

Phytophthora cinnamomi

10.17660/actahortic.2024.1400.9

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First report of Epicoccum nigrum causing disease in Lotus corniculatus in Argentina

New Disease Reports (2018)

María Belén Colavolpe J. Ezquiaga Santiago Javier Maiale Oscar A. Ruíz

Lotus corniculatus is a species adapted to field conditions in the most important cattle production region in Argentina and constitutes a very valuable forage species. In January 2018, black leaf spots were observed on L. corniculatus (Fig. 1) in field plots in Chascomús, Buenos Aires. These spots were amphigenous, circular to irregular, 2-8 mm diameter, close to the leaf margin and reddish brown. Single lesions often coalesced to form larger lesions and became dark brown (Fig. 1). Fungal isolates were obtained from the shoot lesions and were incubated on oatmeal agar, malt extract and potato dextrose agar (Crous et al., 2) at 25°C in darkness. Macroscopic features of colonies on these media and mycelia characteristic were characterised under a microscope (Fig. 2) and conformed morphologically most closely to the genus Epicoccum (Chen et al., 1). Total genomic DNA was extracted from fresh mycelia and PCR amplification was done using the ITS4 and ITS5 primers (White et al., 5) designed to amplify the internal transcribed spacer (ITS) region. The amplified PCR products were purified and sequenced (GenBank Accession No. MH042300). A BLAST search revealed 99% identity to E. nigrum (KX815296 and MF510615). An inoculation trial was done as follows. Lotus corniculatus plants were grown in a growth chamber at 25°C at 30-40% relative humidity with a 12 hr photoperiod. Thirty-day-old leaves were placed on Petri plates containing water agar medium. Leaves were sprayed with a conidia solution prepared by agitating the fungal lawn with distilled water in a petri dish culture, then suspending in 0.05% Tween 20 to a final concentration of 105 conidia/ml. Control leaves were sprayed with water containing 0.05% Tween 20. Petri plates were maintained at 25°C, 100% relative humidity for 16 days. The inoculated L. corniculatus plants were observed to be wilted with a yellow appearance and black spots (Fig. 3). The causal fungus was isolated and the macro- and microscopic characteristics were consistent with the inoculated fungus. The genus Epicoccum was reported in Argentina as a seed pathogen in Lotus spp. (Sisterna & Lori, 4). The isolate in the current study had 98% identity with E. nigrum (KC568289 and KY303832) that caused leaf spot in Lablab purpureus and brown leaf spot in loquat, respectively (Mahadevakumar et al., 3; Wu et al., 6). To our knowledge, this is the first report of E. nigrum causing leaf spot in L. corniculatus in Argentina.

Lotus corniculatus

Potato dextrose agar

Spots

10.5197/j.2044-0588.2018.038.006

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Use of Ozone as a Substrate Treatment for the Control of Trichoderma in the Production of Pleurotus ostreatus

Current Microbiology (2024)

Santiago Jaramillo Mejía María Belén Colavolpe M. Ganuza Edgardo Albertó

Pleurotus ostreatus

Trichoderma

10.1007/s00284-024-03691-z

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European and American chestnuts: An overview of the main threats and control efforts

Frontiers in Plant Science (2022)

Patrícia Fernandes María Belén Colavolpe Susana Serrazina Rita Costa

Chestnuts are multipurpose trees significant for the economy and wildlife. These trees are currently found around the globe, demonstrating their genetic adaptation to different environmental conditions. Several biotic and abiotic stresses have challenged these species, contributing to the decline of European chestnut production and the functional extinction of the American chestnut. Several efforts started over the last century to understand the cellular, molecular, and genetic interactions behind all chestnut biotic and abiotic interactions. Most efforts have been toward breeding for the primary diseases, chestnut blight and ink disease caused by the pathogens, Cryphonectria parasitica and Phytophthora cinnamomi , respectively. In Europe and North America, researchers have been using the Asian chestnut species, which co-evolved with the pathogens, to introgress resistance genes into the susceptible species. Breeding woody trees has several limitations which can be mostly related to the long life cycles of these species and the big genome landscapes. Consequently, it takes decades to improve traits of interest, such as resistance to pathogens. Currently, the availability of genome sequences and next-generation sequencing techniques may provide new tools to help overcome most of the problems tree breeding is still facing. This review summarizes European and American chestnut’s main biotic stresses and discusses breeding and biotechnological efforts developed over the last decades, having ink disease and chestnut blight as the main focus. Climate change is a rising concern, and in this context, the adaptation of chestnuts to adverse environmental conditions is of extreme importance for chestnut production. Therefore, we also discuss the abiotic challenges on European chestnuts, where the response to abiotic stress at the genetic and molecular level has been explored.

Cryphonectria

Chestnut Blight

Tree breeding

10.3389/fpls.2022.951844

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The Potential of Nitrogen-Fixing Bacteria in the Sustainability of Agro-Forestry Ecosystems

Sustainability in plant and crop protection (2019)

Isabel Videira e Castro Márcia de Castro Silva Concepción Albarracín Fernández María Belén Colavolpe Helena Machado

Phytophthora cinnamomi

10.1007/978-3-030-17597-9_5

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Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

AFRICAN JOURNAL OF BIOTECHNOLOGY (2017)

O. Adoyo Gaston María Belén Colavolpe Alberto Edgardo

Spawn quality is an important factor that affects the final yield in mushroom production. One of the most common risks is the contamination of substrates and spawn by mitosporic fungi. Some species do not easily grow on grain substrate as traditional species do and these find it real difficult to produce spawn. A method was evaluated to produce spawn for mushroom production based on aseptically encapsulated mycelium using alginate beads. Strains of four selected edible mushrooms were cultivated in liquid medium, mycelium was harvested by filtration. Subsequently, it was blended with 100 mL of 2% (w/v) sodium alginate solution or with the alginate solution with the addition of 2% (w/v) yeast extract solution or with the addition of 2% (w/v) meat extract solution. An electronic mixer was used to disaggregate the mycelium and generate a homogeneous suspension. To obtain the artificial spawn, the cells suspensions were dripped in 200 mL of 500 mM CaCl2 solution using a cut micro-tip. Beads obtained were inoculated in potato dextrose agar Petri dishes and in substrate (wheat straw or Poplar sawdust) to evaluate the growth. Tests carried out using this method, demonstrated that the incorporation of a suitable nutrient increases the ability of selected mushroom to emerge from the beads. Mycelia colonization tests were carried out on sterilized substrate, one using alginate beads and another one using the traditional spawn as inoculum. No significant differences according to t-test (p<0.05) were shown. However, spawning production based on alginate beads can be performed in only a few days, whereas the traditional method requires more time. The method proposed here represents an antecedent to improve spawn generation for future industrial applications, for further studies and for other uses as mycelium inoculant. Key words: Alginate beads, inoculum, mushroom cultivation, spawn production.

Spawn (biology)

Sawdust

10.5897/ajb2017.16065

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OVARIAN RESPONSE IN BRAHMAN COWS IN POSTPARTUM ANESTRUS FED WITH ENSILED OF SORGHUM

International Journal of Agriculture Environment and Bioresearch (2020)

Analco Cisneros Gerardo María Benedicta Bottini Luzardo María Belén Colavolpe R Rioboo García

Objective: To evaluate the restarting of ovarian cyclicity and the pre-ovulatory follicle diameter of cows in early postpartum period fed sorghum ensiled with sowing without fertilization (T1) and fertilized (T2).Design / Methodology / Approach: Completely randomized design.The response variables were, presence of corpus luteum (CL), as indicative of restarting ovarian activity, and subsequent development of a follicle pre -ovulatory for both revisions ultrasound scanning was used.The data obtained was analyzed with PROC GLM of SAS (SAS, 2011) the measures were compared with the Tukey test (α= 0.05).Results: In the 23 cows fed sorghum ensiled with fertilized sowing(T2) CL and pre -ovulatory follicle were observed.In the case of T1, only 12 cows developed corpus luteum and preovulatory follicle.The diameter measure of the follicles of the cows in (T2) was 18.1mm, while T1 was 0.78mm.Study limitations/implications: With the results observed in the present study, it can be inferred that the management of the crop, in this case the crop of sorghum, has effects on the ovarian reactivation of Brahman cows in the early postpartum period, which implies that the producer you must take care of the feeding of the cattle, from the moment of sowing until it reaches the mouth of animal.Findings/conclusions: According to the results, it is concluded that feeding with ensiled of fertilized sorghum during sowing to cows in the early postpartum period could favor the restart of the estrous cycle and increase the diameter of the follicles.

Brahman

10.35410/ijaeb.2020.5537

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