Valeriy V. Pak

Institute of Bioorganic Chemistry

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Dae Young Kwon

Hoseo University

Minseon Koo

Korea Food Research Institute

Olim K. Khojimatov

Ilia State University

L. M. Yun

Yunusov Institute of the Chemistry of Plant Substances

Vsevolod V. Belousov

Institute of Bioorganic Chemistry

Yulia G. Ermakova

European Molecular Biology Laboratory

ШШ

Ш. Ш. Сагдуллаев

Yunusov Institute of the Chemistry of Plant Substances

Aleksandr V. Pak

National University of Uzbekistan

Gulnara J. Abdiniyazova

Karakalpak State University

Dmitry S. Bilan

Pirogov Russian National Research Medical University

Cooperative Institutions

Lomonosov Moscow State University

Institute of Bioorganic Chemistry

Korea Food Research Institute

Pirogov Russian National Research Medical University

Russian Academy of Sciences

Centre National de la Recherche Scientifique

Moscow State University

Université Paris Sciences et Lettres

Inserm

Centre Interdisciplinaire de Recherche en Biologie

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Conformational analysis of yvae peptide derivatives

Chemistry of Natural Compounds (2012)

Valeriy V. Pak Dae Young Kwon L. M. Yun Abulimiti Yili Haji Akber Aisa

10.1007/s10600-012-0399-6

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Citations (0)

Conformation Analysis of Ile-Ala-Val-Pro Peptide and Its Derivatives by Circular Dichroism

Chemistry of Natural Compounds (2004)

Valeriy V. Pak Minseon Koo Dae Young Kwon T. D. Kasimova

Sequence (biology)

10.1023/b:conc.0000048257.95951.88

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Citations (15)

Centaurea behen L., Centaurea cyanus L. - ASTERACEAE

Ethnobiology (2023)

Olim K. Khojimatov Valeriy V. Pak Rainer W. Bussmann

Centaurea

10.1007/978-3-031-23031-8_21

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Citations (1)

The Mitochondria-to-Cytosol H2O2 Gradient Is Caused by Peroxiredoxin-Dependent Cytosolic Scavenging

Antioxidants (2021)

Laura de Cubas Valeriy V. Pak Vsevolod V. Belousov José Ayté Elena Hidalgo

Fluorescent protein-based reporters used to measure intracellular H2O2 were developed to overcome the limitations of small permeable dyes. The two major families of genetically encoded redox reporters are the reduction-oxidation sensitive green fluorescent protein (roGFP)-based proteins fused to peroxiredoxins and HyPer and derivatives. We have used the most sensitive probes of each family, roGFP2-Tpx1.C169S and HyPer7, to monitor steady-state and fluctuating levels of peroxides in fission yeast. While both are able to monitor the nanomolar fluctuations of intracellular H2O2, the former is two-five times more sensitive than HyPer7, and roGFP2-Tpx1.C169S is partially oxidized in the cytosol of wild-type cells while HyPer7 is fully reduced. We have successfully expressed HyPer7 in the mitochondrial matrix, and it is ~40% oxidized, suggesting higher steady-state levels of peroxides, in the low micromolar range, than in the cytosol. Cytosolic HyPer7 can detect negligible H2O2 in the cytosol from mitochondrial origin unless the main H2O2 scavenger, the cytosolic peroxiredoxin Tpx1, is absent, while mitochondrial HyPer7 is oxidized to the same extent in wild-type and ∆tpx1 cells. We conclude that there is a bidirectional flux of H2O2 across the matrix and the cytosol, but Tpx1 rapidly and efficiently scavenges mitochondrial-generated peroxides and stops their steady-state cytosolic levels rising.

Mitochondrial matrix

Scavenger

Peroxiredoxin

10.3390/antiox10050731

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Citations (26)

중엉아시아 실크로드의 고도 사마르칸트

소대화 Valeriy V. Pak

Source

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Citations (0)

Structures of the Peptide Leu-Pro-Tyr-Pro and Its Derivatives and the Nicotinamide Part of NADPH by a Semi-Empirical PM3 Method

Chemistry of Natural Compounds (2005)

Valeriy V. Pak Meng-Yu Ku Na Li M. S. Kim T. D. Kasymova

Active center

Peptide bond

Bond cleavage

10.1007/s10600-005-0077-z

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Citations (7)

Fermented Soybean Products and Their Bioactive Compounds

InTech eBooks (2011)

Hye Jeong Jeong Sunmin Park Valeriy V. Pak Kyung Rhan Dae Young

Hye Jeong Yang1, Sunmin Park2, Valeriy Pak3, Kyung Rhan Chung4 and Dae Young Kwon1 1Biogeron Food Research Division, Korea Food Research Institute, Songnam, 2Department of Food and Nutrition, Obesity/Diabetes Research Institutes, Hoseo University, Asan, 3Department of Organic Synthesis Institute of the Chemistry of Plant Substances, Tashkent, 4The Academy of Korean Studies, Center for Knowledge & Information, Songnam 1,2,4Republic of Korea 3Uzbekistan

Research center

10.5772/10670

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Citations (35)

In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

Redox Biology (2021)

Ilya V. Kelmanson Arina G. Shokhina Daria A. Kotova M. S. Pochechuev Alexandra D. Ivanova

Ischemic cerebral stroke is one of the leading causes of death and disability in humans. However, molecular processes underlying the development of this pathology remain poorly understood. There are major gaps in our understanding of metabolic changes that occur in the brain tissue during the early stages of ischemia and reperfusion. In particular, it is generally accepted that both ischemia (I) and reperfusion (R) generate reactive oxygen species (ROS) that cause oxidative stress which is one of the main drivers of the pathology, although ROS generation during I/R was never demonstrated in vivo due to the lack of suitable methods. In the present study, we record for the first time the dynamics of intracellular pH and H

Rodent model

Stroke

Dynamics

10.1016/j.redox.2021.102178

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Citations (33)

Design of a highly potent inhibitory peptide acting as a competitive inhibitor of HMG-CoA reductase

Amino Acids (2012)

Valeriy V. Pak Minseon Koo Dae Young Kwon L. M. Yun

10.1007/s00726-012-1276-0

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Citations (47)

Recognized sequence and conformation in design of linear peptides as a competitive inhibitor for HMG‐CoA reductase

Journal of Molecular Recognition (2007)

Valeriy V. Pak Minseon Koo L. M. Yun Dae Young Kwon

Abstract This study is an attempt to develop a simple search method for lead peptide candidates, which include constrained structures in a recognized sequence, using the design of a competitive inhibitor for HMG‐CoA reductase (HMGR). A structure‐functional analysis of previously synthesized peptides proposes that a competitive inhibitory peptide can be designed by maintaining bioactive conformation in a recognized sequence. A conformational aspect of the structure‐based approach was applied to the peptide design. By analysis of the projections obtained through a principle component analysis (PCA) for short linear and cyclic peptides, a head‐to‐tail peptide cycle is considered as a model for its linear analogy. It is proposed that activities of the linear peptides based on an identical amino acid sequence, which are obtained from a less flexible peptide cycle, would be relatively higher than those obtained from more flexible cyclic peptides. The design criterion was formulated in terms of a ‘ V ’ parameter, reflecting a relative deviation of an individual peptide cycle from an average statistical peptide cycle based on all optimized structures of the cyclic peptides in set. Twelve peptide cycles were selected for the peptide library. Comparing the calculated ‘ V ’ parameters, two cyclic peptides (GLPTGG and GFPTGG) were selected as lead cycles from the library. Based on these sequences, six linear peptides obtained by breaking the cycle at different positions were selected as lead peptide candidates. The linear GFPTGG peptide, showing the highest inhibitory activity against HMGR, increases the inhibitory potency nearly tenfold. Kinetic analysis reveals that the GFPTGG peptide is a competitive inhibitor of HMG‐CoA with an equilibrium constant of inhibitor binding ( K i ) of 6.4 ± 0.3 µM. Conformational data support a conformation of the designed peptides close to the bioactive conformation of the previously synthesized active peptides. Copyright © 2007 John Wiley & Sons, Ltd.

Cyclic peptide

Sequence (biology)

Rational design

10.1002/jmr.824

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Citations (18)